目的:探讨趋化因子CXCL4在结直肠癌组织中的临床意义。方法:160例结直肠癌患者根据国际抗癌联盟(UICC)的TNM分期分为Ⅰ、Ⅱ、Ⅲ、Ⅳ期,采用RT-PCR方法定量检测CXCL4 m RNA的表达,应用免疫组织化学法和Western blot检测结直肠癌组织及癌...目的:探讨趋化因子CXCL4在结直肠癌组织中的临床意义。方法:160例结直肠癌患者根据国际抗癌联盟(UICC)的TNM分期分为Ⅰ、Ⅱ、Ⅲ、Ⅳ期,采用RT-PCR方法定量检测CXCL4 m RNA的表达,应用免疫组织化学法和Western blot检测结直肠癌组织及癌旁正常组织CXCL4蛋白表达水平,探讨CXCL4的表达与结直肠病理因素之间的关系。结果:CXCL4 mRNA在结直肠癌组织中的平均相对表达量低于癌旁正常组织,差异具有统计学意义(P<0.05)。CXCL4蛋白在结直肠癌组织中的阳性表达率为86.2%(138/160)。在结直肠组织中,CXCL4的表达与肿瘤大小、分化程度、淋巴结转移、TNM分期相关(P<0.05)。结论:趋化因子CXCL4与结直肠癌的一些临床病理因素密切相关,可能成为评估结直肠癌预后的生物学指标之一。展开更多
Our previous study found that plate factor-4 variant (CXCL4L1) was downregulated in the serum of patients with prostate cancer (PCa). The aim of the present study was to investigate the prognostic value of CXCL4L1 in ...Our previous study found that plate factor-4 variant (CXCL4L1) was downregulated in the serum of patients with prostate cancer (PCa). The aim of the present study was to investigate the prognostic value of CXCL4L1 in PCa. In total, 213 PCa patients treated with radical prostatectomy were enrolled and peripheral blood samples of all patients were collected. Expression of serum CXCL4L1 in patients with different tumor stages and grades were measured by enzyme-linked immunosorbent assay (ELISA). The Kaplan-Meier method was applied to estimate the progression to castration-resistant prostate cancer (CRPC), metastasis, biochemical recurrenee (BCR)-free survival, and overall survival (OS). Prog no stic factors for BCR-free survival and OS were determi ned by univariate and multivariate analyses using the Cox proportional hazards regression model. The expression of CXCL4L1 was significantly lower in PCa patie nts with adva need pathological tumor stage, high-grade Gleason score, and metastasis. Moreover, down regulation of CXCL4L1 not only strongly correlated with aggressive clinicopathological features, but also predicted tumor progress!on and unfavorable outcomes. Finally, multivariate Cox regression analyses identified CXCL4L1 as an independent prognostic factor for both BCR-free survival (hazard ratio [HR]: 2.03, 95% confidence interval [Cl]: 1.26-3.27;P = 0.004) and OS (HR: 2.26, 95% Cl: 1.07-4.79;P= 0.033). In con elusion, our results in dicate that CXCL4L1 might serve as a no vel and promising prog no stic biomarker for patients with PCa and potential therapeutic target in the future.展开更多
Background:Sex is an independent risk factor for systemic sclerosis(SSc).Chemokine ligand 4(CXCL4)participates in SSc via multiple mechanisms.In the present study,we investigated the role of CXCL4 in sex-specific lung...Background:Sex is an independent risk factor for systemic sclerosis(SSc).Chemokine ligand 4(CXCL4)participates in SSc via multiple mechanisms.In the present study,we investigated the role of CXCL4 in sex-specific lung injury in the bleomycin-induced systemic sclerosis(BLM-SSc)mouse model.Methods:Thirty-two 6-week-old CXCL4^(-/-)C57BL/6J and wild-type(WT)mice were divided into CXCL4^(-/-)male,CXCL4^(-/-)female,wild-type male(WT male),and wild-type female(WT female)groups(n=8 each).BLM-SSc mice were established by subcutaneous injections of 100μL(2 mg/mL of bleomycin,once every other day)for 4 weeks.Thereafter,all mice were sacrificed and the extracted lung tissues were stained with hematoxylin and eosin and Masson's trichrome to evaluate the degree of inflammation and fibrosis.Enzyme-linked immunosorbent assay was used to measure the levels of tumor necrosis factor-αand interleukin-6 in the lung tissue homogenate.In addition,immunofluorescence double staining detected the coexpression of S100 calcium-binding protein A8 and matrix metalloproteinase 7.Two-way analysis of variance was used to examine the independent effects of sex,CXCL4,and their interactions.Results:Compared to CXCL4^(-/-)mice,WT mice had significantly higher infiltration of macrophages and neutrophils,inflammation scores,and fibrosis scores(all p<0.05).Specifically,the degree of macrophage infiltration and fibrosis scores were higher in male mice than those in female mice(all p<0.05).In CXCL4^(-/-)mice,fibrosis scores were higher in males than those in females(p<0.05);however,no significant difference was observed in inflammation scores between the two groups(p>0.05).Compared with that of the CXCL4^(-/-)mice,the levels of tumor necrosis factor-αand interleukin-6 in the lung tissue homogenate were increased in the WT mice with higher levels in the male group.No significant sex-based differences were observed in CXCL4^(-/-)mice(p>0.05).Immunofluorescence double staining revealed a significantly higher number of positive doublestained cells in the WT male than the WT female group;however,no such cells were observed in CXCL4^(-/-)groups.Conclusion:CXCL4 may participate in the pathogenesis of sex-based differential lung injury in the BLM-SSc model.展开更多
文摘目的:探讨趋化因子CXCL4在结直肠癌组织中的临床意义。方法:160例结直肠癌患者根据国际抗癌联盟(UICC)的TNM分期分为Ⅰ、Ⅱ、Ⅲ、Ⅳ期,采用RT-PCR方法定量检测CXCL4 m RNA的表达,应用免疫组织化学法和Western blot检测结直肠癌组织及癌旁正常组织CXCL4蛋白表达水平,探讨CXCL4的表达与结直肠病理因素之间的关系。结果:CXCL4 mRNA在结直肠癌组织中的平均相对表达量低于癌旁正常组织,差异具有统计学意义(P<0.05)。CXCL4蛋白在结直肠癌组织中的阳性表达率为86.2%(138/160)。在结直肠组织中,CXCL4的表达与肿瘤大小、分化程度、淋巴结转移、TNM分期相关(P<0.05)。结论:趋化因子CXCL4与结直肠癌的一些临床病理因素密切相关,可能成为评估结直肠癌预后的生物学指标之一。
基金the National Natural Science Foundation of China (No. 81600258 and 81802540)the Natural Science Foundation of Liaoning Province (No. 20180550985)the Shenyang Science and Technology Program (No. F15-199-1-47).
文摘Our previous study found that plate factor-4 variant (CXCL4L1) was downregulated in the serum of patients with prostate cancer (PCa). The aim of the present study was to investigate the prognostic value of CXCL4L1 in PCa. In total, 213 PCa patients treated with radical prostatectomy were enrolled and peripheral blood samples of all patients were collected. Expression of serum CXCL4L1 in patients with different tumor stages and grades were measured by enzyme-linked immunosorbent assay (ELISA). The Kaplan-Meier method was applied to estimate the progression to castration-resistant prostate cancer (CRPC), metastasis, biochemical recurrenee (BCR)-free survival, and overall survival (OS). Prog no stic factors for BCR-free survival and OS were determi ned by univariate and multivariate analyses using the Cox proportional hazards regression model. The expression of CXCL4L1 was significantly lower in PCa patie nts with adva need pathological tumor stage, high-grade Gleason score, and metastasis. Moreover, down regulation of CXCL4L1 not only strongly correlated with aggressive clinicopathological features, but also predicted tumor progress!on and unfavorable outcomes. Finally, multivariate Cox regression analyses identified CXCL4L1 as an independent prognostic factor for both BCR-free survival (hazard ratio [HR]: 2.03, 95% confidence interval [Cl]: 1.26-3.27;P = 0.004) and OS (HR: 2.26, 95% Cl: 1.07-4.79;P= 0.033). In con elusion, our results in dicate that CXCL4L1 might serve as a no vel and promising prog no stic biomarker for patients with PCa and potential therapeutic target in the future.
基金Nanjing Medical University Science and Technology Development Fund,Grant/Award Number:NMUB20220211Huai'an Natural Science Research Program,Grant/Award Number:HAP202308。
文摘Background:Sex is an independent risk factor for systemic sclerosis(SSc).Chemokine ligand 4(CXCL4)participates in SSc via multiple mechanisms.In the present study,we investigated the role of CXCL4 in sex-specific lung injury in the bleomycin-induced systemic sclerosis(BLM-SSc)mouse model.Methods:Thirty-two 6-week-old CXCL4^(-/-)C57BL/6J and wild-type(WT)mice were divided into CXCL4^(-/-)male,CXCL4^(-/-)female,wild-type male(WT male),and wild-type female(WT female)groups(n=8 each).BLM-SSc mice were established by subcutaneous injections of 100μL(2 mg/mL of bleomycin,once every other day)for 4 weeks.Thereafter,all mice were sacrificed and the extracted lung tissues were stained with hematoxylin and eosin and Masson's trichrome to evaluate the degree of inflammation and fibrosis.Enzyme-linked immunosorbent assay was used to measure the levels of tumor necrosis factor-αand interleukin-6 in the lung tissue homogenate.In addition,immunofluorescence double staining detected the coexpression of S100 calcium-binding protein A8 and matrix metalloproteinase 7.Two-way analysis of variance was used to examine the independent effects of sex,CXCL4,and their interactions.Results:Compared to CXCL4^(-/-)mice,WT mice had significantly higher infiltration of macrophages and neutrophils,inflammation scores,and fibrosis scores(all p<0.05).Specifically,the degree of macrophage infiltration and fibrosis scores were higher in male mice than those in female mice(all p<0.05).In CXCL4^(-/-)mice,fibrosis scores were higher in males than those in females(p<0.05);however,no significant difference was observed in inflammation scores between the two groups(p>0.05).Compared with that of the CXCL4^(-/-)mice,the levels of tumor necrosis factor-αand interleukin-6 in the lung tissue homogenate were increased in the WT mice with higher levels in the male group.No significant sex-based differences were observed in CXCL4^(-/-)mice(p>0.05).Immunofluorescence double staining revealed a significantly higher number of positive doublestained cells in the WT male than the WT female group;however,no such cells were observed in CXCL4^(-/-)groups.Conclusion:CXCL4 may participate in the pathogenesis of sex-based differential lung injury in the BLM-SSc model.
