Objective: To observe the therapeutic e ffect of injection of "Chuan-Shen-Tong" Injectio (川参通注射液) at Huiyin (会阴 CV 1) on benign hyperplasia of prostate . Methods: 60 cases of benign hyperplasia of pr...Objective: To observe the therapeutic e ffect of injection of "Chuan-Shen-Tong" Injectio (川参通注射液) at Huiyin (会阴 CV 1) on benign hyperplasia of prostate . Methods: 60 cases of benign hyperplasia of prostate patients were evenly randomized into treatment group and control group. Those of treatment group were treated with injection o f "Chuan-Shen-Tong" Injectio (composed of Rhizoma Ligustici Chuanxiong, Rad ix Salviae Miltiorrhizae, etc. for relieving hyperplasis of prostate, 4 mL/acupoin t) into Huiyin (CV 1) and the other 60 cases of control group were treated by oral administration of Hytrin (1 mg/d in the 1 st week and increasing 1 mg/3 d, 4 mg at m ost). After 28 days’ treatment, their therapeutic effects were compared. Result s: After treatment, of each 60 cases in treatment and control groups, 11 (18.3%) and 11 (11.7%) were controlled in their symptoms, 25 (41.7%) and 16 (26.7%) had marked improvement, 18 (30.0%) and 17 (28.3%) had improvement, and 6 (10.0%) an d 20 (33.3%) failed, with the effective rate and the markedly effective rate bei ng 90.0% and 66.7% in the treatment group and 66.7% and 38.3% in the control gro up respectively. The therapeutic effect of treatment group was significantly sup erior to that of control group (P<0.01 and P< 0.05). Conclusion: The therapeutic effect of point-injection of "Chuan-Shen-Tong" for benign hyperplasia of p rostate is better than that of oral administration of Hytrin.展开更多
The CVI （coastal vulnerability index） was developed and used to assess the vulnerability of the coastline of the Kingdom of Bahrain main islands to future SLR （sea level rise）. A total of 717 km of the coastline w...The CVI （coastal vulnerability index） was developed and used to assess the vulnerability of the coastline of the Kingdom of Bahrain main islands to future SLR （sea level rise）. A total of 717 km of the coastline was evaluated. Six spatial factors acting on the coastal area： erosion/accretion patterns （shoreline change）, topography （elevation above mean sea level）, geology, geomorphology, slope, and mean sea level rise were incorporated and ranked to develop the CVI. This index was classified into four levels of vulnerability： low, moderate, high, and very high. Vulnerable hotspots are located along the central portions of the western and eastern coastlines. The vulnerability of these areas is mostly driven by their characteristically shallow coastal slopes, low elevations, and erosion-prone nature of the sandy soils presents, comprising about 54 km of the studied shoreline. Another 33 km of coastline were classified as highly vulnerable and located along the eastern coast. In addition, the western coast of the southern tip of the main island （Bahrain） was also classified as a highly vulnerable shoreline. Twenty-two km was classified as the moderate vulnerable. The remaining coastal areas were classified as low to moderately vulnerable comprising about 608 km of the total length of the coastline. Identifying those hotspots susceptible to SLR is essential for more effective coastal zone management and to help in reducing the impacts of SLR on both infrastructure and human beings.展开更多
OBJECTIVE: To investigate the effect of catgut implantation at acupoints on the expressions of γ-amino butyric acid B receptor(GABAB ) and metabotropic glutamate receptor 1(mGluR1) in the brain stem of rats with spas...OBJECTIVE: To investigate the effect of catgut implantation at acupoints on the expressions of γ-amino butyric acid B receptor(GABAB ) and metabotropic glutamate receptor 1(mGluR1) in the brain stem of rats with spasticity after stroke.METHODS: In total, 60 male Sprague-Dawley rats were randomly divided into three groups: a sham group(n=10), a model group(n=25) and a treatment group(n=25). The rats in both the model group and the treatment group were subjected to middle cerebral artery occlusion to establish a model of focal cerebral ischemia. Rats with limb-spasm met the inclusion criteria. Only the left carotid artery was isolated in sham group rats. Three days after modeling, the treatment group was subjected to catgut implantation at Dazhui(GV 14), Guanyuan(CV 4), and Zhongwan(CV 12). Neurological deficit symptoms were assessed with the Zea-Longa neurological deficit score. The Modified Ashworth Scale(MAS), and isolated muscle tone were used to evaluate spasticity before and after treatment. Immunohistochemistry was applied to determine the expression of GABAB and mGluR1 in the rat brain stem after treatment.RESULTS: After treatment, neural impairment symptoms had significantly improved in the treatment group when compared to the model group(P<0.05). Both MAS and isolated muscle tone in the treatment group were significantly decreased when compared with the model group(P<0.05),and were also lower than before treatment. GABAB expression was significantly higher and mGluR1 was lower in the treatment group when compared with the model group(P<0.01 and P<0.05, respectively).CONCLUSION: Catgut implantation at Dazhui(GV 14), Guanyuan(CV 4), and Zhongwan(CV 12), can relieve limb spasticity by increasing the expression of GABAB and reducing the expression of mGluR1 in the brain stem of rats after stroke.展开更多
Gastrointestinal(GI)cancers are a leading cause of cancer morbidity and mortality worldwide.Despite advances in treatment,cancer relapse remains a significant challenge,necessitating novel therapeutic strategies.In th...Gastrointestinal(GI)cancers are a leading cause of cancer morbidity and mortality worldwide.Despite advances in treatment,cancer relapse remains a significant challenge,necessitating novel therapeutic strategies.In this study,we engineered nanobody-based chimeric antigen receptor(CAR)natural killer(NK)cells targeting cadherin 17(CDH17)for the treatment of GI tumors.In addition,to enhance the efficacy of CAR-NK cells,we also incorporated CV1,a CD47–SIRPαaxis inhibitor,to evaluate the anti-tumor effect of this combination.We found that CDH17-CAR-NK cells effectively eliminated GI cancers cells in a CDH17-dependent manner.CDH17-CAR-NK cells also exhibit potent in vivo anti-tumor effects in cancer cell-derived xenograft and patient-derived xenograft mouse models.Additionally,the anti-tumor activity of CDH17-CAR-NK cells is synergistically enhanced by CD47–signal regulatory proteinα(SIRPα)axis inhibitor CV1,likely through augmented macrophages activation and an increase in M1-phenotype macrophages in the tumor microenvironment.Collectively,our findings suggest that CDH17-targeting CAR-NK cells are a promising strategy for GI cancers.The combination of CDH17-CAR-NK cells with CV1 emerges as a potential combinatorial approach to overcome the limitations of CAR-NK therapy.Further investigations are warranted to speed up the clinical translation of these findings.展开更多
Mulberry is rich in bioactive substances,which might undergo biotransformation through microbial fermentation,and therefore show great prospect in improving the bioavailability of these substances.We studied the diffe...Mulberry is rich in bioactive substances,which might undergo biotransformation through microbial fermentation,and therefore show great prospect in improving the bioavailability of these substances.We studied the differential metabolites in mulberry juices fermented by Pichia kudriavzevii S1 and Weissella confusa L1 alone or in combination.The sensory analysis using electronic nose and electronic tongue showed that fermentation caused higher response value of aromatic components,reduced bitterness and bitter aftertaste of the juices.The differential metabolites of juices identified by non-targeted metabolomics showed that the contents of total anthocyanins,total flavonoids and total polyphenols in juices fermented by W.confusa and P.kudriavzevii increased by 13.34%,43.75%and 9.40%,respectively.a total of 10 categories involving 111 kinds of key metabolites were identified in four different treatment groups.These metabolites were mostly involved in amino acid,flavonoid and anthocyanin metabolism pathways.This study might offer new pathways for improving the functional features of mulberry juices through microbial fermentation.展开更多
By using acupoint injection with antibiotics into Huiyin （CV 1） and the prostate,we treated 122 cases of chronic prostatitis.The report is now given as follows.1 Clinical Data 1.1 Diagnostic criteria The diagnostic ...By using acupoint injection with antibiotics into Huiyin （CV 1） and the prostate,we treated 122 cases of chronic prostatitis.The report is now given as follows.1 Clinical Data 1.1 Diagnostic criteria The diagnostic criteria were referred to the involved studies [1,2].（1） Urgent,展开更多
This study investigated the effect of weight percent gain （WPG） and compression set on the crystallity of compressed poplar wood. Tests were conducted to determine the mechanism of compressed wood set. The samples o...This study investigated the effect of weight percent gain （WPG） and compression set on the crystallity of compressed poplar wood. Tests were conducted to determine the mechanism of compressed wood set. The samples of 1-72 Poplar were treated with a PF resin of water-solubility and low molecular weight. Then the air-dried samples were hot-pressed to a certain thickness. Finally the specimens were obtained from the compressed samples and scanned with an X-ray diffractometer. Results show that before press the crystallity of the inner layer is lower than that of the outer layer, and at press the difference of the crystallity between the outer layer and the inner layer will slowly disappear and finally the crystallity of the inner layer exceeds that of the outer layer; with the increasing compression set the crystallity decreases, and there is a big decrease; the crystallity difference between two layers happens because of compression set gradient, WPG difference or the joint effect.展开更多
The variation of tensile strength parallel to grain in Chinese fir and 1-214 poplar wood from plantations was studied in this paper. Aaccording to the national standards The Testing Methods for Physical and Mechanical...The variation of tensile strength parallel to grain in Chinese fir and 1-214 poplar wood from plantations was studied in this paper. Aaccording to the national standards The Testing Methods for Physical and Mechanical Properties of Wood （GB 1927 to 1943 -- 1991）, the small clear wood samples were cut and tested, which represent the south and north of trees with heights of 1.3, 3.3, 5.3 and 7.3 m. The results showed： the tensile strength parallel to grain of the north wood was higher than that of south wood. ANOVA shows that the tensile strength parallel to the grain with height is of significant difference （at 0.05 level）.展开更多
文摘Objective: To observe the therapeutic e ffect of injection of "Chuan-Shen-Tong" Injectio (川参通注射液) at Huiyin (会阴 CV 1) on benign hyperplasia of prostate . Methods: 60 cases of benign hyperplasia of prostate patients were evenly randomized into treatment group and control group. Those of treatment group were treated with injection o f "Chuan-Shen-Tong" Injectio (composed of Rhizoma Ligustici Chuanxiong, Rad ix Salviae Miltiorrhizae, etc. for relieving hyperplasis of prostate, 4 mL/acupoin t) into Huiyin (CV 1) and the other 60 cases of control group were treated by oral administration of Hytrin (1 mg/d in the 1 st week and increasing 1 mg/3 d, 4 mg at m ost). After 28 days’ treatment, their therapeutic effects were compared. Result s: After treatment, of each 60 cases in treatment and control groups, 11 (18.3%) and 11 (11.7%) were controlled in their symptoms, 25 (41.7%) and 16 (26.7%) had marked improvement, 18 (30.0%) and 17 (28.3%) had improvement, and 6 (10.0%) an d 20 (33.3%) failed, with the effective rate and the markedly effective rate bei ng 90.0% and 66.7% in the treatment group and 66.7% and 38.3% in the control gro up respectively. The therapeutic effect of treatment group was significantly sup erior to that of control group (P<0.01 and P< 0.05). Conclusion: The therapeutic effect of point-injection of "Chuan-Shen-Tong" for benign hyperplasia of p rostate is better than that of oral administration of Hytrin.
文摘The CVI （coastal vulnerability index） was developed and used to assess the vulnerability of the coastline of the Kingdom of Bahrain main islands to future SLR （sea level rise）. A total of 717 km of the coastline was evaluated. Six spatial factors acting on the coastal area： erosion/accretion patterns （shoreline change）, topography （elevation above mean sea level）, geology, geomorphology, slope, and mean sea level rise were incorporated and ranked to develop the CVI. This index was classified into four levels of vulnerability： low, moderate, high, and very high. Vulnerable hotspots are located along the central portions of the western and eastern coastlines. The vulnerability of these areas is mostly driven by their characteristically shallow coastal slopes, low elevations, and erosion-prone nature of the sandy soils presents, comprising about 54 km of the studied shoreline. Another 33 km of coastline were classified as highly vulnerable and located along the eastern coast. In addition, the western coast of the southern tip of the main island （Bahrain） was also classified as a highly vulnerable shoreline. Twenty-two km was classified as the moderate vulnerable. The remaining coastal areas were classified as low to moderately vulnerable comprising about 608 km of the total length of the coastline. Identifying those hotspots susceptible to SLR is essential for more effective coastal zone management and to help in reducing the impacts of SLR on both infrastructure and human beings.
基金Supported by Henan University of Traditional Chinese Medcine Innovation Team Projet(No.2011XCXTD05)
文摘OBJECTIVE: To investigate the effect of catgut implantation at acupoints on the expressions of γ-amino butyric acid B receptor(GABAB ) and metabotropic glutamate receptor 1(mGluR1) in the brain stem of rats with spasticity after stroke.METHODS: In total, 60 male Sprague-Dawley rats were randomly divided into three groups: a sham group(n=10), a model group(n=25) and a treatment group(n=25). The rats in both the model group and the treatment group were subjected to middle cerebral artery occlusion to establish a model of focal cerebral ischemia. Rats with limb-spasm met the inclusion criteria. Only the left carotid artery was isolated in sham group rats. Three days after modeling, the treatment group was subjected to catgut implantation at Dazhui(GV 14), Guanyuan(CV 4), and Zhongwan(CV 12). Neurological deficit symptoms were assessed with the Zea-Longa neurological deficit score. The Modified Ashworth Scale(MAS), and isolated muscle tone were used to evaluate spasticity before and after treatment. Immunohistochemistry was applied to determine the expression of GABAB and mGluR1 in the rat brain stem after treatment.RESULTS: After treatment, neural impairment symptoms had significantly improved in the treatment group when compared to the model group(P<0.05). Both MAS and isolated muscle tone in the treatment group were significantly decreased when compared with the model group(P<0.05),and were also lower than before treatment. GABAB expression was significantly higher and mGluR1 was lower in the treatment group when compared with the model group(P<0.01 and P<0.05, respectively).CONCLUSION: Catgut implantation at Dazhui(GV 14), Guanyuan(CV 4), and Zhongwan(CV 12), can relieve limb spasticity by increasing the expression of GABAB and reducing the expression of mGluR1 in the brain stem of rats after stroke.
基金funded by the National Key Research and Development Program of China(2022YFC2303600)the Key Special Project of“Strategic Science and Technology Innovation Cooperation”from National Key R&D Program(2023YFE0204500,China)+11 种基金Shenzhen Science and Technology Innovation Committee(RCBS20210706092213007,China)the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2023D003,CI2021B014,and CI2023D008,China)the CACMS Innovation Fund(CI2023E002,CI2021A05101,and CI2021A05104,China)the Science and Technology Foundation of Shenzhen(JCYJ20210324115800001,JCYJ20210324113608023,and JCYJ20200109120205924,China)the Science and Technology Foundation of Shenzhen(Shenzhen Clinical Medical Research Center for Geriatric Diseases)the Shenzhen Medical Research Fund(B2302051,China)the Shenzhen Science and Technology Innovation Committee(SZSTI)(RCYX20221008092950121,China)the National Natural Science Foundation of China(82373775,32101219,82103140,and 82304518)the Natural Science Foundation of Top Talent of SZTU(GDRC202125,China)the HUILAN Public Welfare,the Shenzhen Science and Technology Innovation Commission(JCYJ20200109120205924,China)Shenzhen Key Medical Discipline Construction Fund(SZXK046,China)Guangdong Basic and Applied Basic Research Foundation(2021A1515012164,2022A1515110745,and 2021A1515111188,China)International Science and Technology Cooperation for Shenzhen Technology Innovation Plan(GJHZ20200731095411034,China).
文摘Gastrointestinal(GI)cancers are a leading cause of cancer morbidity and mortality worldwide.Despite advances in treatment,cancer relapse remains a significant challenge,necessitating novel therapeutic strategies.In this study,we engineered nanobody-based chimeric antigen receptor(CAR)natural killer(NK)cells targeting cadherin 17(CDH17)for the treatment of GI tumors.In addition,to enhance the efficacy of CAR-NK cells,we also incorporated CV1,a CD47–SIRPαaxis inhibitor,to evaluate the anti-tumor effect of this combination.We found that CDH17-CAR-NK cells effectively eliminated GI cancers cells in a CDH17-dependent manner.CDH17-CAR-NK cells also exhibit potent in vivo anti-tumor effects in cancer cell-derived xenograft and patient-derived xenograft mouse models.Additionally,the anti-tumor activity of CDH17-CAR-NK cells is synergistically enhanced by CD47–signal regulatory proteinα(SIRPα)axis inhibitor CV1,likely through augmented macrophages activation and an increase in M1-phenotype macrophages in the tumor microenvironment.Collectively,our findings suggest that CDH17-targeting CAR-NK cells are a promising strategy for GI cancers.The combination of CDH17-CAR-NK cells with CV1 emerges as a potential combinatorial approach to overcome the limitations of CAR-NK therapy.Further investigations are warranted to speed up the clinical translation of these findings.
基金supported by the Natural Science Foundation of Heilongjiang Province(LH2021c075),Key Laboratory of Functional inorganic Material chemistry(Heilongjiang University),Ministry of Education.
文摘Mulberry is rich in bioactive substances,which might undergo biotransformation through microbial fermentation,and therefore show great prospect in improving the bioavailability of these substances.We studied the differential metabolites in mulberry juices fermented by Pichia kudriavzevii S1 and Weissella confusa L1 alone or in combination.The sensory analysis using electronic nose and electronic tongue showed that fermentation caused higher response value of aromatic components,reduced bitterness and bitter aftertaste of the juices.The differential metabolites of juices identified by non-targeted metabolomics showed that the contents of total anthocyanins,total flavonoids and total polyphenols in juices fermented by W.confusa and P.kudriavzevii increased by 13.34%,43.75%and 9.40%,respectively.a total of 10 categories involving 111 kinds of key metabolites were identified in four different treatment groups.These metabolites were mostly involved in amino acid,flavonoid and anthocyanin metabolism pathways.This study might offer new pathways for improving the functional features of mulberry juices through microbial fermentation.
文摘By using acupoint injection with antibiotics into Huiyin （CV 1） and the prostate,we treated 122 cases of chronic prostatitis.The report is now given as follows.1 Clinical Data 1.1 Diagnostic criteria The diagnostic criteria were referred to the involved studies [1,2].（1） Urgent,
文摘This study investigated the effect of weight percent gain （WPG） and compression set on the crystallity of compressed poplar wood. Tests were conducted to determine the mechanism of compressed wood set. The samples of 1-72 Poplar were treated with a PF resin of water-solubility and low molecular weight. Then the air-dried samples were hot-pressed to a certain thickness. Finally the specimens were obtained from the compressed samples and scanned with an X-ray diffractometer. Results show that before press the crystallity of the inner layer is lower than that of the outer layer, and at press the difference of the crystallity between the outer layer and the inner layer will slowly disappear and finally the crystallity of the inner layer exceeds that of the outer layer; with the increasing compression set the crystallity decreases, and there is a big decrease; the crystallity difference between two layers happens because of compression set gradient, WPG difference or the joint effect.
基金This work was supported by State Science and Technology Infrastructure Platform Projects (2004DEA70900).
文摘The variation of tensile strength parallel to grain in Chinese fir and 1-214 poplar wood from plantations was studied in this paper. Aaccording to the national standards The Testing Methods for Physical and Mechanical Properties of Wood （GB 1927 to 1943 -- 1991）, the small clear wood samples were cut and tested, which represent the south and north of trees with heights of 1.3, 3.3, 5.3 and 7.3 m. The results showed： the tensile strength parallel to grain of the north wood was higher than that of south wood. ANOVA shows that the tensile strength parallel to the grain with height is of significant difference （at 0.05 level）.
