Neptunea cumingii is an important nutrition-rich economic species in China.Juveniles of N.cumingii suff er from high mortality at low temperatures,which has proved a limiting factor in raising seedlings in artifi cial...Neptunea cumingii is an important nutrition-rich economic species in China.Juveniles of N.cumingii suff er from high mortality at low temperatures,which has proved a limiting factor in raising seedlings in artifi cial habitats.Previous research has shown that N.cumingii displays aggregation behavior in response to adverse environmental changes.Therefore,we determined the eff ects of temperature,food,size of juvenile snails,substratum type,and density of juvenile snails on the aggregation behavior of N.cumingii.Results show that,at a low(4°C)or a high(22°C)temperature,juvenile snails adjusted to the inhospitable environment by exhibiting increased aggregation behavior.However,their aggregation behaviors diff ered at these two temperatures.There was no signifi cant diff erence in the aggregation rate,but the typical aggregation size was larger at 4°C than at 22°C.At 10°C or 16°C,aggregation behavior of juvenile snails reduced.Aggregation increased in the satiation treatment at 10°C and 16°C.Small-sized juveniles tended to have higher aggregation rates(92.22%)and a larger typical aggregation size.More juveniles were distributed in the bottom of shaded substrata.A larger typical aggregation size or higher density signifi cantly reduced the mortality of juvenile snails at a low temperature(4°C).These results broaden our understanding of gastropod aggregation behavior and can be used to develop and improve commercial breeding strategies and resource recovery for N.cumingii.展开更多
Plasma Membrane Calcium ATPase (PMCA) plays a critical role in transporting Ca<sup>2+</sup> out of the cytosol across the plasma membrane. Here, a full-length cDNA sequence of plasma membrane Ca<sup>...Plasma Membrane Calcium ATPase (PMCA) plays a critical role in transporting Ca<sup>2+</sup> out of the cytosol across the plasma membrane. Here, a full-length cDNA sequence of plasma membrane Ca<sup>2+</sup>-ATPase gene was isolated from the gill of <em>Hyriopsis cumingii</em> (HcPMCA) by using SMART RACE technique. The entire cDNA was 5230 bp, including a 417-bp 5'-UTR, a 3588-bp ORF and a 1225-bp 3'-UTR, encoding a 1195-amino acid protein, and no putative signal peptide was predicted. Compared with PMCA homologs from seawater mollusks, HcPMCA had high similarity with them in both sequence and structure. Tissue-specific expression analysis revealed that HcPMCA mRNA was detected in all the sampled tissues, but was prominently expressed in the gill and mantle. When exposed to a serie of increasing Ca<sup>2+</sup> that lasted for 7 days, the mRNA expression of HcPMCA in the mantle was slightly downregulated, but peaked at 60 mg/L. Moreover, the temporal expression of HcPMCA transcripts in the mantle after 60 mg/L Ca<sup>2+</sup> exposure was shown to be bell-shaped, which was slightly downregulated at 24 h, but upregulated from 24 h to 48 h post-treatment, peaking at 48 h. The result of present study provides useful information for further studies on function and regulation mechanism of HcPMCA gene.展开更多
基金Supported by the National Key R&D Program of China(No.2019YFD0900800)the fund earmarked for the Modern Agro-industry Technology Research System(No.CARS-49)+5 种基金the Major Scientifi c and Technological Innovation Project of Shandong Provincial Key Research and Development Program(No.2019JZZY020708)the Industry Leading Talents Project of Taishan Scholars(to ZHANG Tao),the Double-Hundred Blue Industry Leader Team of Yantai(to ZHANG Tao)the Construction Technology and Demonstration Application of Dandong Characteristic Beach Type Marine Ranch(No.KFJ-STS-QYZD-189)the Shandong Peninsula Coastal Area Ecological Simulation Test Project:Marine Ranch Resource Evaluation and Sustainable Utilization Model ResearchNSFC-Shandong Joint Fund for Marine Science Research Centers(No.U1406403)the Creative Team Project of the Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology(No.LMEES-CTSP-2018-1)。
文摘Neptunea cumingii is an important nutrition-rich economic species in China.Juveniles of N.cumingii suff er from high mortality at low temperatures,which has proved a limiting factor in raising seedlings in artifi cial habitats.Previous research has shown that N.cumingii displays aggregation behavior in response to adverse environmental changes.Therefore,we determined the eff ects of temperature,food,size of juvenile snails,substratum type,and density of juvenile snails on the aggregation behavior of N.cumingii.Results show that,at a low(4°C)or a high(22°C)temperature,juvenile snails adjusted to the inhospitable environment by exhibiting increased aggregation behavior.However,their aggregation behaviors diff ered at these two temperatures.There was no signifi cant diff erence in the aggregation rate,but the typical aggregation size was larger at 4°C than at 22°C.At 10°C or 16°C,aggregation behavior of juvenile snails reduced.Aggregation increased in the satiation treatment at 10°C and 16°C.Small-sized juveniles tended to have higher aggregation rates(92.22%)and a larger typical aggregation size.More juveniles were distributed in the bottom of shaded substrata.A larger typical aggregation size or higher density signifi cantly reduced the mortality of juvenile snails at a low temperature(4°C).These results broaden our understanding of gastropod aggregation behavior and can be used to develop and improve commercial breeding strategies and resource recovery for N.cumingii.
文摘Plasma Membrane Calcium ATPase (PMCA) plays a critical role in transporting Ca<sup>2+</sup> out of the cytosol across the plasma membrane. Here, a full-length cDNA sequence of plasma membrane Ca<sup>2+</sup>-ATPase gene was isolated from the gill of <em>Hyriopsis cumingii</em> (HcPMCA) by using SMART RACE technique. The entire cDNA was 5230 bp, including a 417-bp 5'-UTR, a 3588-bp ORF and a 1225-bp 3'-UTR, encoding a 1195-amino acid protein, and no putative signal peptide was predicted. Compared with PMCA homologs from seawater mollusks, HcPMCA had high similarity with them in both sequence and structure. Tissue-specific expression analysis revealed that HcPMCA mRNA was detected in all the sampled tissues, but was prominently expressed in the gill and mantle. When exposed to a serie of increasing Ca<sup>2+</sup> that lasted for 7 days, the mRNA expression of HcPMCA in the mantle was slightly downregulated, but peaked at 60 mg/L. Moreover, the temporal expression of HcPMCA transcripts in the mantle after 60 mg/L Ca<sup>2+</sup> exposure was shown to be bell-shaped, which was slightly downregulated at 24 h, but upregulated from 24 h to 48 h post-treatment, peaking at 48 h. The result of present study provides useful information for further studies on function and regulation mechanism of HcPMCA gene.
