Background As the global population increases,the demand for protein sources is expected to increase,driving the demand for cell-based cultivated meat.This study aimed to enhance the productivity of cultivated meat th...Background As the global population increases,the demand for protein sources is expected to increase,driving the demand for cell-based cultivated meat.This study aimed to enhance the productivity of cultivated meat through optimization of the cell source and organization process.Results We engineered fibroblasts into myogenic cells via non-viral introduction of the MYOD1 gene,avoiding viral methods for safety.After confirming the stable derivation of myogenic cells,we combined knockout(KO)of MSTN,a negative regulator of myogenesis,with MYOD1-mediated myogenesis to improve cultivated meat production.Primary cells from MSTN KO cattle exhibited enhanced myogenic potential.Additionally,when tested in immortalized fibroblasts,myostatin treatment reduced MYOD1-induced myogenesis in two-dimensional cultures,while MSTN knockout increased it.To achieve muscle-like cell alignment,we employed digital light processing(DLP)-based three-dimensional(3D)bioprinting to organize cells into 3D groove-shaped hydrogels.These bioactive hydrogels supported stable cell proliferation and significantly improved muscle cell alignment.Upon differentiation into myotubes,the cells demonstrated an ordered alignment,particularly the MSTN KO cells,which showed highly efficient differentiation.Conclusions The integration of genetic modification and advanced DLP 3D bioprinting with groove-patterned hydrogels provides an effective strategy for producing high-quality,muscle-aligned cultivated meat.展开更多
Taxus cuspidata,a rare species of the Taxus genus,and its wild resources are under severe threat.The development of cultivated species has become an important strategy to replace wild species.The objective of this wor...Taxus cuspidata,a rare species of the Taxus genus,and its wild resources are under severe threat.The development of cultivated species has become an important strategy to replace wild species.The objective of this work was to elucidate the differences in secondary metabolite accumulation,particularly in the paclitaxel biosynthesis pathway,between wild and cultivated species.This study employed liquid chromatography-tandem mass spectrometry(LC-MS/MS)and RNA sequencing(RNA-Seq)technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T.cuspidata.The results showed that the content of paclitaxel in cultivated species was significantly higher than in wild species,reaching 1.67 times that of the latter(p 0.01).Additionally,the<content of key paclitaxel precursors,GGPP and 10-deacetylbaccatin III,in cultivated species was 1.94 times(p 0.05)<and 1.71 times(p 0.01)higher than in wild species,respectively.Transcriptomic analysis identified 2606 differentially<expressed genes(DEGs),among which key enzyme genes related to paclitaxel biosynthesis(such as DXS,DXR,GGPS,etc.)were generally upregulated in cultivated species.Multiple key enzyme genes in both the 2-C-methyl-D-erythritol 4-phosphate pathway(MEP)and paclitaxel biosynthesis pathways were significantly upregulated in cultivated species.Conversely,genes and metabolites related to sugar metabolism were found to be higher in content in wild species.These findings reveal the significant advantage of cultivated species in paclitaxel production capacity,providing new insights into the metabolic regulation mechanisms during yew domestication.This has important implications for optimizing paclitaxel biosynthesis and guiding future improvements in T.cuspidata cultivars.展开更多
The parasitic dodder(Cuscuta,Convolvulaceae)species have wide ranges of hosts.However,some plants,including the cultivated tomato(Solanum lycopersicum),have different degrees of resistance to Cuscuta.The cultivated to...The parasitic dodder(Cuscuta,Convolvulaceae)species have wide ranges of hosts.However,some plants,including the cultivated tomato(Solanum lycopersicum),have different degrees of resistance to Cuscuta.The cultivated tomato plants activate a strong hypersensitive response(HR)where Cuscuta haustoria penetrate stems of cultivated tomato,but the underlying mechanisms by which the cultivated tomato perceives Cuscuta and activates resistance remain unclear.In this study,we show that the phytohormones jasmonic acid(JA)and salicylic acid(SA)in cultivated tomato stems were highly induced by Cuscuta australis parasitization.Genetic analyses and experiments of supplementation of JA or SA indicated that the JA and SA pathway not only are both required for activation of HR against Cuscuta parasitization but also function in non-HR-based resistance.The Cuscuta Receptor 1(CuRe1),which is a leucine-rich repeat receptor-like protein,and suppressor of BAK1-interacting receptor kinase(SOBIR1)and SOBIR1-like,two adaptor kinases,are also important for HR-based and non-HR-based resistance.Importantly,we found that the JA and SA pathway both transcriptionally regulate CuRe1.However,in the cure1 mutants,JA and SA levels were still normally induced by C.australis parasitization.We propose a linear model that an unknown receptor perceives Cuscuta parasitization and thus triggers accumulation of JA and SA,which in turn induce the transcription of CuRe1,and CuRe1 and SOBIR1/SOBIR1-like thereby activate HR-based and non-HR-based resistance to Cuscuta.This study underscores the important roles of hormone signaling and resistance(R)genes in host plant-parasitic plant interactions.展开更多
A substantial body of research has been conducted with the objective of enhancing the understanding of Non-Timber Forest Products (NTFP) in Central Africa. A significant proportion of the studies focus on medicinal pl...A substantial body of research has been conducted with the objective of enhancing the understanding of Non-Timber Forest Products (NTFP) in Central Africa. A significant proportion of the studies focus on medicinal plants, in contrast to those that examine food products. Nevertheless, studies on edible wild mushrooms as NTFPs and cultivated mushrooms, on their economic potential and value chains in the Great Lakes Region, remain scarce and largely absent for some NTFPs. In light of the aforementioned considerations, the present study aims to examine the economic potential and value chain of the production sector of wild edible mushrooms (WEM) and cultivated mushrooms (CM) within the Virunga National Park (PNVi) and its surrounding areas in North Kivu, Democratic Republic of the Congo (DRC). To this end, a sample of 432 respondents was selected, distributed equitably among the actors of the Goma-Kitshanga-Mweso value chain, the Goma-Rutshuru chain, the Beni-Mutwanga chain, and the Beni-Mangina chain. A digital questionnaire consisting of open and closed questions was administered, and observations were carried out concurrently. The quantities of mushrooms sold were weighed and photographed. The results demonstrated that, when the eight-month fruiting period of wild and cultivated mushrooms was considered as a single year for all actors within the value chain, a picker could sell 6.1 kg at $2 and earn $12. A seller could sell 16 kg at $4 and earn $64, while a grower could sell 502 kg at $3 and earn $1506. Consequently, the CM grower generates a significantly higher profit than other participants in the value chain, despite the expenses he incurs. Ultimately, the domestication of WEM, highly prized by the local population of the Virunga National Park, has the potential to enhance their household incomes, as evidenced by the findings of this study.展开更多
On 18 December 2023,a MS 6.2 earthquake struck the Jishishan area in Northwest China,located at the border of the Qinghai-Tibet and Loess Plateau.The earthquake triggered shallow loess landslides,small rock failures,a...On 18 December 2023,a MS 6.2 earthquake struck the Jishishan area in Northwest China,located at the border of the Qinghai-Tibet and Loess Plateau.The earthquake triggered shallow loess landslides,small rock failures,and soil cracks,mainly along hilly gullies and cut slopes at the edges of terraced fields.A rare large-scale flowslide also occurred in irrigated farmland.These seismic landslides and collapses blocked roads,buried farmland,damaged houses,and resulted in many casualties.Field investigations revealed that these geological hazards were concentrated around cultivated land.Consequently,cultivated land was introduced as an engineering geological zoning factor into the seismic geological hazard risk assessment for Jishishan area.The Newmark cumulative displacement model was refined by incorporating lithological uncertainties via the Monte Carlo method.Comparative analysis of coseismic geohazards with and without considering cultivated land suggests that,in loess-covered areas with cultivation activities,the consideration of the disturbed characteristics of soils provides a more accurate probabilistic risk assessment of seismic geohazards.Human cultivation and irrigation activities affect the physical properties of surface soil,the terraced fields around earthquake prone areas have a risk of earthquake-induced geological hazards.This study may offer valuable insights for hazard prevention and mitigation in high fortification intensity loess covered areas.展开更多
Sustainable intensification of cultivated land use(SICLU) and large-scale operations(LSO) are widely acknowledged strategies for enhancing agricultural performance.However,the existing literature has faced challenges ...Sustainable intensification of cultivated land use(SICLU) and large-scale operations(LSO) are widely acknowledged strategies for enhancing agricultural performance.However,the existing literature has faced challenges in precisely defining SICLU and constructing comprehensive indicators,which has hindered the exploration of factors influencing LSO within the SICLU framework.To address this gap,we integrated self-efficacy theory into the design of an index framework for evaluating SICLU.We subsequently employed econometric models to analyze the significant factors that impact LSO.Our findings reveal that SICLU can be divided into four key dimensions:intensive management,efficient output,resource conservation,and ecological environment optimization.Furthermore,it is crucial to incorporate belief-based cognitive factors into the index system,as farmers’ understanding of fertilizer and pesticide application significantly influences their willingness to engage in LSO.Moreover,we identify grain market turnover as the most influential factor in promoting LSO,with single-factor contribution rates reaching 70.9% for cultivated land transfer willingness and 62.5% for the total planting areas.Interestingly,unlike irrigation and agricultural machinery inputs,increased labor inputs correspond to larger planting areas for farmers.This trend may be attributed to reduced labor availability because of rural labor migration,whereas the reduction in irrigation and agricultural input is contingent on innovations in production practices and the transfer of cultivated land management rights.Importantly,SICLU dynamically influences LSO,with each index related to SICLU having an optimal range that fosters LSO.These insights offer valuable guidance for policymakers,emphasizing farmers as their central focus,with the adjustment of input and output factors as a means to achieve LSO as the ultimate goal.In conclusion,we propose research avenues for further enriching the SICLU framework to ensure that it aligns with the specific characteristics of regional agricultural development.展开更多
[Objective]The aim of this study was to set up a high performance liquid chromatography for rapid determination of isoflavones from soybean and analyze the correlation between isofalvone content and protein or fat con...[Objective]The aim of this study was to set up a high performance liquid chromatography for rapid determination of isoflavones from soybean and analyze the correlation between isofalvone content and protein or fat content. [Method]The isoflavones were firstly extracted by 80% methanol and then hydrolyzed at 100 ℃. The chromatographic separation adopted a reversed-phase C18 analytical column with binary high-pressure gradient elution,while its analysis time was 25 min and column temperature was 40 ℃. The diode array detector was used for monitoring with wavelength of 260 nm. The correlation between isofalvone content and protein or fat content was analyzed by data processing system Origin 6.0. [Result]The high performance liquid chromatograph for determination of isoflavones from soybean was verified to be accurate and reliable by methodology. The isoflavones of 85 soybean lines cultivated in Jilin Province were determined,and the results primarily showed the characters and ranges of isoflavones from soybean lines cultivated in Jilin Province,while the isoflavone content of soybeans ranged from 2.29 to 4.89 mg/g,and the average content was 3.36 mg/g. The isoflavone content of 5 soybean lines exceeded 4 mg/g,while there was a remarkably negative correlation between isoflavone content and protein content,and there was no significant positive correlation between isoflavone content and fat content. [Conclusion]The isoflavone content of soybean lines cultivated in Jilin Province is higher,so it is feasible for breeding the soybean lines with high isoflavone content and fat contetnt.展开更多
At present,the quality protection and early warning of cultivated land in China are actually based on the increase and decrease of the quantity of cultivated land and the change of its quality.The early warning of cul...At present,the quality protection and early warning of cultivated land in China are actually based on the increase and decrease of the quantity of cultivated land and the change of its quality.The early warning of cultivated land quality based on this is easy to cause the problem of missing and wrong alarm.Based on the quality and productivity change of regional cultivated land,early warning on the regional cultivated land quality is conducted in this study respectively,and the results of the two early warning are compared.Moreover,a multi-indicator cultivated land quality early warning based on the quality change,productivity change and auxiliary indicators is proposed.The results show that:early warning of cultivated land quality based on quality and productivity change of cultivated land has certain limitations.Based on multi-indicator early warning,the number of counties with heavy,medium and light warnings in Henan Province is 52,8 and 11 respectively.The multi-indicator early warning result of cultivated land quality is more accurate,which can provide a basis for cultivated land protection and management assessment,and has great application value.展开更多
Northeast China as one of important agricultural production bases is an area under reclamation and returning cultivated land to forests or pastures. Therefore, it is of great practical significance in guaranteeing the...Northeast China as one of important agricultural production bases is an area under reclamation and returning cultivated land to forests or pastures. Therefore, it is of great practical significance in guaranteeing the sustainable development and national food security to study the spatial and temporal variation of cultivated land in Northeast China under future climate scenarios. In this study, based on data of land use, natural environment and social-economy, dynamics of land system（DLS） model was used to to simulate the spatial distribution and changing trends of cultivated land in the typical areas of reclamation and returning cultivated land to forest or pastures in Northeast China during 2010-2030 under land use planning scenario and representative concentration pathways（RCPs） scenarios quantitatively.The results showed that the area of cultivated land had an overall decreasing trend under the land use planning scenario, but the area of upland field increased slightly from 2000 to 2010 and then declined greatly, while the area of paddy field continuously declined from 2000 to 2030. Under the Asia-Pacific Integrated model（AIM）scenario, the total area of cultivated land had a tendency to increase considerably,with the upland field expanding more obviously and the paddy field declining slightly.In addition, the cultivated land showed a greater decreasing trend under the model for energy supply strategy alternatives and their general environmental impact（MESSAGE） scenario compared to the land use planning scenario. Moreover, analysis on the conversion between different land use types indicated that the reclamation and returning cultivated land to forests or pastures was likely to continue under future scenarios, but the frequency of occurrence could decrease as the time goes by. The conclusions can provide significant decision-making information for the rational agricultural planning and cultivated land protection in Northeast China to adapt to the climate change.展开更多
Using gradually regression analysis to establish the driving force model of utilized change of cultivated land in Gonghe County, and using path analysis, correlation analysis, partial correlation analysis and system d...Using gradually regression analysis to establish the driving force model of utilized change of cultivated land in Gonghe County, and using path analysis, correlation analysis, partial correlation analysis and system dynamics method to inspect the effect of driving changing on cultivated land change under different change situations. Driving factors, action mechanism and process of utilized change of cultivated land were analyzed from the county territory scale level. At last, some corresponding policies and measures were put forward.展开更多
The first internal transcribed spacer(ITS1) of nuclear ribosomal DNA of three wild rice species and two subspecies of cultivated rice, which are distributed in China, was amplified using PCR technique and sequenced wi...The first internal transcribed spacer(ITS1) of nuclear ribosomal DNA of three wild rice species and two subspecies of cultivated rice, which are distributed in China, was amplified using PCR technique and sequenced with automated fluorescent sequencing. The sequences of ITS1 ranged from 193 bp to 218 bp in size and G/C content varied from 69.3% to 72.7%. In pairwise comparisons among the five taxa, sequence site divergence ranged from 1.5% to 10.6%. Phylogenetic analysis of ITS1 sequences using Wagner parsimony generated a single well resolved tree, which revealed that Oryza rufipogon was much more closely related to cultivated rice species than to the other two wild species. Oryza granulata was less closely related to either cultivated rice species or the other two wild species, and might be a unique and isolated taxon in the genus Oryza. The phylogenetic relationships of the three wild rice species and two cultivated rice subspecies inferred from ITS1 sequences is highly concordant with those based on the molecular evidence from isozyme, chloroplast DNA (cpDNA), mitochondrial DNA(mtDNA) and nuclear DNA (nDNA) of the genus Oryza .展开更多
[ Objective] The genetic diversity of the local cultivated aromatic rice and non-aromatic rice in Yunnan Province were compared to provide further genetic resources for breeding practice. [Method] Genetic diversity of...[ Objective] The genetic diversity of the local cultivated aromatic rice and non-aromatic rice in Yunnan Province were compared to provide further genetic resources for breeding practice. [Method] Genetic diversity of 10 aromatic rice and 45 non-aromatic rice were analyzed by 64 SSR primers covered on 12 rice chromosomes. [ Result] Per locus 5.44 and 7.98 alleles in average were detected, ranging from 2 to 12 and from 2 to 17 in aromatic and non-aromatic rice, respectively. Average genetic multiplicity index（Hs） was 0.46 and 0.67 respectively. The average polymorphism information content （PIC） was 0.43 and 0.58 in aromatic and non-aromatic rice respectively. [ Conclusion] The results indicated that genetic diversity was higher in non-aromatic rice than in aromatic rice.展开更多
Introduction The plants of genus Cistanche G. Beck, which belong to Orobanchaceae family, include about twenty species, and there are four species and one variety are distributed in the northwest part of China. The d...Introduction The plants of genus Cistanche G. Beck, which belong to Orobanchaceae family, include about twenty species, and there are four species and one variety are distributed in the northwest part of China. The dried fleshy stem of Cistanche genus plant known as ＇Roucongrong＇ in Chinese traditional medicine, has long been used for kidney deficiency, female infertility, morbid leucorrhea, and neurasthenia. Studies on active components and pharmacological activities show that it possesses significant activities in enhancing potency, anti-fatigue, immuno-modulability, etc, and phenylethanoid glycosides are the major active components. Due to excessive exploitation, its natural resources are facing with exhaustion. In order to improve this situation, the plant has been cultivated on a large scale in the northwest part of China. Up to now, the chemical investigations of the cultivated Cistartcbe salsa have not been undertaken. In this paper we report seven phenylethanoid glycosides from the cultivated Cistanche salsa： echinacoside （1）, cistanoside A （2）, acteoside （3）, isoacteoside （4）, 2＇-acetylacteoside （5）, tubuloside B （6）, and eutigoside A （7）. Among them, compound 7 isolated fronl family Orobanchaceae was reported previously, and compounds 1, 2, 3, 4, 5 and 6 from the cultivated Cistanche salsa are reported for the first time.展开更多
[Objective] To study the chemic component of rice cell wall among different varieties and at different rice growth stages.[Method] The contents of neutral detergent fiber（NDF）,acid detergent fiber（ADF） and acid de...[Objective] To study the chemic component of rice cell wall among different varieties and at different rice growth stages.[Method] The contents of neutral detergent fiber（NDF）,acid detergent fiber（ADF） and acid detergent lignin （ADL） of rice were analyzed by Van Soest Method.[Result] The contents of NDF,ADF and ADL varied from 55.63% to 71.70%,31.98% to 49.61% and 2.68% to 5.71% respectively,the highest content was gained in Dongxiang wide rice at dough stage,while the lowest content was obtained in rice heading stage of Xieqingzao B.In the same growth stage,the contents of NDF（except heading stage） and ADF were significantly（P0.05 or 0.01） difference among three varieties of rice,the highest content was Dongxiang wide rice,followed by Xiushui 110,and the lowest was Xieqingzao B.In the same variety rice,the content of NDF and ADF were significantly （P0.05 or 0.01） difference among three rice growth stages,the lowest contents was rice heading stage,followed by rice milk ripe stage,the highest was rice dough stage.[Conclusion] the NDF,ADF and other contents of indica rice were lower than that of ordinary wild rice and japonica rice,therefore,it owned a certain value in use of straw feed.If the indica rice could be harvest as earlier as possible,the rice straw together with silage fermentation and enzymatic hydrolysis could provide the effective diet for livestock.展开更多
Using the multi-temporal Landsat data and survey data of national resources, the authors studied the dynamics of cultivated land and landcover changes of typical ecological regions in China. The results of investigati...Using the multi-temporal Landsat data and survey data of national resources, the authors studied the dynamics of cultivated land and landcover changes of typical ecological regions in China. The results of investigation showed that the whole distribution of the cultivated land shifted to Northeast and Northwest China, and as a result, the ecological quality of cultivated land dropped down. The seacoast and cultivated land in the area of Yellow River Mouth expanded by an increasing rate of 0.73 kma-1, with a depositing rate of 2.1 kma-1. The desertification area of the dynamic of Horqin Sandy Land increased from 60.02% of the total land area in1970s to 64.82% in1980s but decreased to 54.90% in early 1990s. As to the change of North Tibet lakes, the water area of the Namu Lake decreased by 38.58 km2 from year 1970 to 1988, with a decreasing rate of 2.14 km2a-1.展开更多
Using path analysis, correlation analysis, partial correlation analysis and system dynamics method to study the driving force of cultivated land in Qinghai Lake Area, and using gradually regression analysis to establi...Using path analysis, correlation analysis, partial correlation analysis and system dynamics method to study the driving force of cultivated land in Qinghai Lake Area, and using gradually regression analysis to establish the driving force model of utilized change of cultivated land. Driving factors, action mechanism and process of utilized change of cultivated land were analyzed, and the differences during all factors were compared. The study provides some decision basis for sustainable utilization and management of land resources in Qinghai Lake Area.展开更多
[Objective] This paper aimed at exploring the countermeasures for cultivated land protection and food security assurance and providing references for the formulation of policies related to cultivated land and grain pr...[Objective] This paper aimed at exploring the countermeasures for cultivated land protection and food security assurance and providing references for the formulation of policies related to cultivated land and grain production of the whole province.[Methods] Data of population,cultivated land,grain and others in Hunan Province during 1989 to 2008 were collected by county（district）.The changes of cultivated land and grain production of the whole province were analyzed using Geographical Information System（GIS）.[Results] The population was increasing continuously,the total area of cultivated land was reducing as a whole in spite of increasing in one or two year in one or two county,the total grain production and per capita grain yield was fluctuated within a certain range in recent 20 years of the whole province.The reducing of cultivated land was mainly due to the industrialization and urbanization development.The unbalanced quantity and quality of cultivated land,various natural disasters,readjustment of industry structure and inner readjustment in the agriculture,variety improvement and improvement of cultivated technology mainly affected of the grain production.[Conclusions] The protection of the quantity and quality of cultivated land has to be strengthened to ensure food security.It is important to enhance the ability of resisting drought and flood disasters by improving the construction of agriculture establishment in order to insure the productivity of cultivated land,to increase farmer’s enthusiasm for growing grain with favourable food policy,to improve the per unit area yield of grain by variety improvement and to increase grain sown area by enlarging the double-cropping rice sown area.展开更多
Based upon ArCGIS,the vertical spatial transfer law of cultivated land in Yunnan was studied through referring to three periods of remote sensing data of Land-Use and Land-Cover Change（LUCC） in Yunnan in 1986,1995 a...Based upon ArCGIS,the vertical spatial transfer law of cultivated land in Yunnan was studied through referring to three periods of remote sensing data of Land-Use and Land-Cover Change（LUCC） in Yunnan in 1986,1995 and 2000,Digital Elevation Model（DEM） of Yunnan Province and LUCC data in Yunnan from 2000 to 2008.The main factors that promote vertical spatial transfer law of cultivated land were analyzed by using SPSS,and the influence of policies and government acts upon the change of cultivated land was clarified.The findings indicated that for Yunnan cultivated land there is a trend that in the vertical direction steep slope areas and gentle slope areas divert into mid-slope areas.The primary factor influencing the vertical spatial transfer of cultivated land was policies and government acts.Also,the gradient of ground,total population and number of agriculture employed persons were also main factors in influencing the change of cultivated land,whose influence was obviously below policies and government acts.展开更多
[Objective]The aim was to analyze genetic diversity of SSR markers in Hordeum vulgare L.in Qinghai Province and lay a foundation for screening and protecting some excellent H.vulgare cultivars.[Method]SSR markers were...[Objective]The aim was to analyze genetic diversity of SSR markers in Hordeum vulgare L.in Qinghai Province and lay a foundation for screening and protecting some excellent H.vulgare cultivars.[Method]SSR markers were used to evaluate the genetic diversity of 42 cultivated H.vulgare from Qinghai Province.[Result]42 H.vulgare showed polymorphism in 7 SSR markers locus.A total of 24 alleles were identified,and the number of alleles per locus ranged from 1 to 6,with an average of 3.0.According to SSR markers polymorphism,42 H.vulgare could be divided into 4 groups,namely I,II,III and IV.[Result]The study indicated that cultivated H.vulgare from Qinghai Province is rich in genetic diversity,which will provide reference for selecting parent of H.vulgare breeding.展开更多
The conflicts among food security, economic development and ecological protection are the “sticking point” of undeveloped southwestern mountainous areas of China. The objectives of this study are to identify appropr...The conflicts among food security, economic development and ecological protection are the “sticking point” of undeveloped southwestern mountainous areas of China. The objectives of this study are to identify appropriate inte- grated indicators influencing the classification and gradation of cultivated land quality in the southwestern mountainous area of China based on semi-structure interview, and to promote the monitoring of cultivated land quality in this region. Taking Bishan County of Chongqing as a study case, the integrated indicators involve the productivity, protection, ac- ceptability, and stability of cultivated land. The integrated indicators accord with the characteristics of land resources and human preference in southwestern mountainous area of China. In different agricultural zones, we emphasize different indicators, such as emphasizing productivity, stabilization and acceptability in low hilly and plain agricultural integrative zone (LHP-AIZ), protection, productivity and stability in low mountain and hill agro-forestry ecological zone (LMH-AEZ), and acceptability in plain outskirts integrative agricultural zone (PO-IAZ), respectively. The pronounced difference of classification and gradation of cultivated land, regardless of inter-region or intra-region, is observed, with the reducible rank from PO-IAZ, LHP-AIZ to LMH-AEZ. Research results accord with the characteristics of assets management and intensive utilization of cultivated land resources in the southwestern mountainous area of China. Semi-structure interview adequately presents the principal agent of farmers in agricultural land use and rural land market. This method is very effective and feasible to obtain data of the quality of cultivated land in the southwestern mountainous area of China.展开更多
基金financially supported by the Korea Institute of Planning and Evaluation for Technology in Food,Agriculture and Forestry(IPET-RS-2024–00402320)by the Meterials/Parts Technology Development Pro-gram(1415187291,Development of composite formulation with a sustained release(gene)for the treatment of companion animal sarcopenia)funded By the Ministry of Trade,Industry&Energy(MOTIE,Korea)。
文摘Background As the global population increases,the demand for protein sources is expected to increase,driving the demand for cell-based cultivated meat.This study aimed to enhance the productivity of cultivated meat through optimization of the cell source and organization process.Results We engineered fibroblasts into myogenic cells via non-viral introduction of the MYOD1 gene,avoiding viral methods for safety.After confirming the stable derivation of myogenic cells,we combined knockout(KO)of MSTN,a negative regulator of myogenesis,with MYOD1-mediated myogenesis to improve cultivated meat production.Primary cells from MSTN KO cattle exhibited enhanced myogenic potential.Additionally,when tested in immortalized fibroblasts,myostatin treatment reduced MYOD1-induced myogenesis in two-dimensional cultures,while MSTN knockout increased it.To achieve muscle-like cell alignment,we employed digital light processing(DLP)-based three-dimensional(3D)bioprinting to organize cells into 3D groove-shaped hydrogels.These bioactive hydrogels supported stable cell proliferation and significantly improved muscle cell alignment.Upon differentiation into myotubes,the cells demonstrated an ordered alignment,particularly the MSTN KO cells,which showed highly efficient differentiation.Conclusions The integration of genetic modification and advanced DLP 3D bioprinting with groove-patterned hydrogels provides an effective strategy for producing high-quality,muscle-aligned cultivated meat.
基金supported by grants from the National Science Foundation of China to Yanwen Zhang(32272757,31972363)grants from Liaoning Provincial Department of Education Project to Dandan Wang(JYTMS20230698)grants from the Liaoning Provincial Science and Technology.Fund Project to Dandan Wang(2023JH2/101700200).
文摘Taxus cuspidata,a rare species of the Taxus genus,and its wild resources are under severe threat.The development of cultivated species has become an important strategy to replace wild species.The objective of this work was to elucidate the differences in secondary metabolite accumulation,particularly in the paclitaxel biosynthesis pathway,between wild and cultivated species.This study employed liquid chromatography-tandem mass spectrometry(LC-MS/MS)and RNA sequencing(RNA-Seq)technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T.cuspidata.The results showed that the content of paclitaxel in cultivated species was significantly higher than in wild species,reaching 1.67 times that of the latter(p 0.01).Additionally,the<content of key paclitaxel precursors,GGPP and 10-deacetylbaccatin III,in cultivated species was 1.94 times(p 0.05)<and 1.71 times(p 0.01)higher than in wild species,respectively.Transcriptomic analysis identified 2606 differentially<expressed genes(DEGs),among which key enzyme genes related to paclitaxel biosynthesis(such as DXS,DXR,GGPS,etc.)were generally upregulated in cultivated species.Multiple key enzyme genes in both the 2-C-methyl-D-erythritol 4-phosphate pathway(MEP)and paclitaxel biosynthesis pathways were significantly upregulated in cultivated species.Conversely,genes and metabolites related to sugar metabolism were found to be higher in content in wild species.These findings reveal the significant advantage of cultivated species in paclitaxel production capacity,providing new insights into the metabolic regulation mechanisms during yew domestication.This has important implications for optimizing paclitaxel biosynthesis and guiding future improvements in T.cuspidata cultivars.
基金supported by National Natural Science Foundation of China(32270314(GS))the Key Project of Applied Basic Research Program of Yunnan(202201AS070056(JW),202301AS070064(GS))Yunnan Revitalization Talent Support Program“Yunling Scholar”Project(JW),Chinese Academy of Sciences(CAS)Light of West China Program(GS),Yunnan Revitalization Talent Support Program“Young Talents”Project(XDYC-QNRC-2022-0001(GS)).
文摘The parasitic dodder(Cuscuta,Convolvulaceae)species have wide ranges of hosts.However,some plants,including the cultivated tomato(Solanum lycopersicum),have different degrees of resistance to Cuscuta.The cultivated tomato plants activate a strong hypersensitive response(HR)where Cuscuta haustoria penetrate stems of cultivated tomato,but the underlying mechanisms by which the cultivated tomato perceives Cuscuta and activates resistance remain unclear.In this study,we show that the phytohormones jasmonic acid(JA)and salicylic acid(SA)in cultivated tomato stems were highly induced by Cuscuta australis parasitization.Genetic analyses and experiments of supplementation of JA or SA indicated that the JA and SA pathway not only are both required for activation of HR against Cuscuta parasitization but also function in non-HR-based resistance.The Cuscuta Receptor 1(CuRe1),which is a leucine-rich repeat receptor-like protein,and suppressor of BAK1-interacting receptor kinase(SOBIR1)and SOBIR1-like,two adaptor kinases,are also important for HR-based and non-HR-based resistance.Importantly,we found that the JA and SA pathway both transcriptionally regulate CuRe1.However,in the cure1 mutants,JA and SA levels were still normally induced by C.australis parasitization.We propose a linear model that an unknown receptor perceives Cuscuta parasitization and thus triggers accumulation of JA and SA,which in turn induce the transcription of CuRe1,and CuRe1 and SOBIR1/SOBIR1-like thereby activate HR-based and non-HR-based resistance to Cuscuta.This study underscores the important roles of hormone signaling and resistance(R)genes in host plant-parasitic plant interactions.
文摘A substantial body of research has been conducted with the objective of enhancing the understanding of Non-Timber Forest Products (NTFP) in Central Africa. A significant proportion of the studies focus on medicinal plants, in contrast to those that examine food products. Nevertheless, studies on edible wild mushrooms as NTFPs and cultivated mushrooms, on their economic potential and value chains in the Great Lakes Region, remain scarce and largely absent for some NTFPs. In light of the aforementioned considerations, the present study aims to examine the economic potential and value chain of the production sector of wild edible mushrooms (WEM) and cultivated mushrooms (CM) within the Virunga National Park (PNVi) and its surrounding areas in North Kivu, Democratic Republic of the Congo (DRC). To this end, a sample of 432 respondents was selected, distributed equitably among the actors of the Goma-Kitshanga-Mweso value chain, the Goma-Rutshuru chain, the Beni-Mutwanga chain, and the Beni-Mangina chain. A digital questionnaire consisting of open and closed questions was administered, and observations were carried out concurrently. The quantities of mushrooms sold were weighed and photographed. The results demonstrated that, when the eight-month fruiting period of wild and cultivated mushrooms was considered as a single year for all actors within the value chain, a picker could sell 6.1 kg at $2 and earn $12. A seller could sell 16 kg at $4 and earn $64, while a grower could sell 502 kg at $3 and earn $1506. Consequently, the CM grower generates a significantly higher profit than other participants in the value chain, despite the expenses he incurs. Ultimately, the domestication of WEM, highly prized by the local population of the Virunga National Park, has the potential to enhance their household incomes, as evidenced by the findings of this study.
基金funded by the Basic Scientific Research Fund,Science and Technology Innovation Base of Lanzhou,Institute of Earthquake Forecasting,China Earthquake Administration(Grants No.2020IESLZ03 and 2015IESLZ05)the National Key R&D program of China(Grant No.2017YFC1500906)
文摘On 18 December 2023,a MS 6.2 earthquake struck the Jishishan area in Northwest China,located at the border of the Qinghai-Tibet and Loess Plateau.The earthquake triggered shallow loess landslides,small rock failures,and soil cracks,mainly along hilly gullies and cut slopes at the edges of terraced fields.A rare large-scale flowslide also occurred in irrigated farmland.These seismic landslides and collapses blocked roads,buried farmland,damaged houses,and resulted in many casualties.Field investigations revealed that these geological hazards were concentrated around cultivated land.Consequently,cultivated land was introduced as an engineering geological zoning factor into the seismic geological hazard risk assessment for Jishishan area.The Newmark cumulative displacement model was refined by incorporating lithological uncertainties via the Monte Carlo method.Comparative analysis of coseismic geohazards with and without considering cultivated land suggests that,in loess-covered areas with cultivation activities,the consideration of the disturbed characteristics of soils provides a more accurate probabilistic risk assessment of seismic geohazards.Human cultivation and irrigation activities affect the physical properties of surface soil,the terraced fields around earthquake prone areas have a risk of earthquake-induced geological hazards.This study may offer valuable insights for hazard prevention and mitigation in high fortification intensity loess covered areas.
基金Under the auspices of National Natural Science Foundation of China(No.42071226,41671176)Taishan Scholars Youth Expert Support Plan of Shandong Province(No.TSQN202306183)。
文摘Sustainable intensification of cultivated land use(SICLU) and large-scale operations(LSO) are widely acknowledged strategies for enhancing agricultural performance.However,the existing literature has faced challenges in precisely defining SICLU and constructing comprehensive indicators,which has hindered the exploration of factors influencing LSO within the SICLU framework.To address this gap,we integrated self-efficacy theory into the design of an index framework for evaluating SICLU.We subsequently employed econometric models to analyze the significant factors that impact LSO.Our findings reveal that SICLU can be divided into four key dimensions:intensive management,efficient output,resource conservation,and ecological environment optimization.Furthermore,it is crucial to incorporate belief-based cognitive factors into the index system,as farmers’ understanding of fertilizer and pesticide application significantly influences their willingness to engage in LSO.Moreover,we identify grain market turnover as the most influential factor in promoting LSO,with single-factor contribution rates reaching 70.9% for cultivated land transfer willingness and 62.5% for the total planting areas.Interestingly,unlike irrigation and agricultural machinery inputs,increased labor inputs correspond to larger planting areas for farmers.This trend may be attributed to reduced labor availability because of rural labor migration,whereas the reduction in irrigation and agricultural input is contingent on innovations in production practices and the transfer of cultivated land management rights.Importantly,SICLU dynamically influences LSO,with each index related to SICLU having an optimal range that fosters LSO.These insights offer valuable guidance for policymakers,emphasizing farmers as their central focus,with the adjustment of input and output factors as a means to achieve LSO as the ultimate goal.In conclusion,we propose research avenues for further enriching the SICLU framework to ensure that it aligns with the specific characteristics of regional agricultural development.
文摘[Objective]The aim of this study was to set up a high performance liquid chromatography for rapid determination of isoflavones from soybean and analyze the correlation between isofalvone content and protein or fat content. [Method]The isoflavones were firstly extracted by 80% methanol and then hydrolyzed at 100 ℃. The chromatographic separation adopted a reversed-phase C18 analytical column with binary high-pressure gradient elution,while its analysis time was 25 min and column temperature was 40 ℃. The diode array detector was used for monitoring with wavelength of 260 nm. The correlation between isofalvone content and protein or fat content was analyzed by data processing system Origin 6.0. [Result]The high performance liquid chromatograph for determination of isoflavones from soybean was verified to be accurate and reliable by methodology. The isoflavones of 85 soybean lines cultivated in Jilin Province were determined,and the results primarily showed the characters and ranges of isoflavones from soybean lines cultivated in Jilin Province,while the isoflavone content of soybeans ranged from 2.29 to 4.89 mg/g,and the average content was 3.36 mg/g. The isoflavone content of 5 soybean lines exceeded 4 mg/g,while there was a remarkably negative correlation between isoflavone content and protein content,and there was no significant positive correlation between isoflavone content and fat content. [Conclusion]The isoflavone content of soybean lines cultivated in Jilin Province is higher,so it is feasible for breeding the soybean lines with high isoflavone content and fat contetnt.
基金Supported by Henan Youth Science Foundation(212300410168)Basic Scientific Research Project of Henan Academy of Sciences(220601065)。
文摘At present,the quality protection and early warning of cultivated land in China are actually based on the increase and decrease of the quantity of cultivated land and the change of its quality.The early warning of cultivated land quality based on this is easy to cause the problem of missing and wrong alarm.Based on the quality and productivity change of regional cultivated land,early warning on the regional cultivated land quality is conducted in this study respectively,and the results of the two early warning are compared.Moreover,a multi-indicator cultivated land quality early warning based on the quality change,productivity change and auxiliary indicators is proposed.The results show that:early warning of cultivated land quality based on quality and productivity change of cultivated land has certain limitations.Based on multi-indicator early warning,the number of counties with heavy,medium and light warnings in Henan Province is 52,8 and 11 respectively.The multi-indicator early warning result of cultivated land quality is more accurate,which can provide a basis for cultivated land protection and management assessment,and has great application value.
基金Supported by the Major Research Project of National Natural Science Foundation Committee(91325302)China Postdoctoral Foundation(2014M560110)Hebei Social Science Foundation(HB15GL087)~~
文摘Northeast China as one of important agricultural production bases is an area under reclamation and returning cultivated land to forests or pastures. Therefore, it is of great practical significance in guaranteeing the sustainable development and national food security to study the spatial and temporal variation of cultivated land in Northeast China under future climate scenarios. In this study, based on data of land use, natural environment and social-economy, dynamics of land system（DLS） model was used to to simulate the spatial distribution and changing trends of cultivated land in the typical areas of reclamation and returning cultivated land to forest or pastures in Northeast China during 2010-2030 under land use planning scenario and representative concentration pathways（RCPs） scenarios quantitatively.The results showed that the area of cultivated land had an overall decreasing trend under the land use planning scenario, but the area of upland field increased slightly from 2000 to 2010 and then declined greatly, while the area of paddy field continuously declined from 2000 to 2030. Under the Asia-Pacific Integrated model（AIM）scenario, the total area of cultivated land had a tendency to increase considerably,with the upland field expanding more obviously and the paddy field declining slightly.In addition, the cultivated land showed a greater decreasing trend under the model for energy supply strategy alternatives and their general environmental impact（MESSAGE） scenario compared to the land use planning scenario. Moreover, analysis on the conversion between different land use types indicated that the reclamation and returning cultivated land to forests or pastures was likely to continue under future scenarios, but the frequency of occurrence could decrease as the time goes by. The conclusions can provide significant decision-making information for the rational agricultural planning and cultivated land protection in Northeast China to adapt to the climate change.
基金Supported by the National Social Science Fund(06XMZ014)~~
文摘Using gradually regression analysis to establish the driving force model of utilized change of cultivated land in Gonghe County, and using path analysis, correlation analysis, partial correlation analysis and system dynamics method to inspect the effect of driving changing on cultivated land change under different change situations. Driving factors, action mechanism and process of utilized change of cultivated land were analyzed from the county territory scale level. At last, some corresponding policies and measures were put forward.
基金The project supported by the Grant from Presidentof Chinese Academy of Sciences
文摘The first internal transcribed spacer(ITS1) of nuclear ribosomal DNA of three wild rice species and two subspecies of cultivated rice, which are distributed in China, was amplified using PCR technique and sequenced with automated fluorescent sequencing. The sequences of ITS1 ranged from 193 bp to 218 bp in size and G/C content varied from 69.3% to 72.7%. In pairwise comparisons among the five taxa, sequence site divergence ranged from 1.5% to 10.6%. Phylogenetic analysis of ITS1 sequences using Wagner parsimony generated a single well resolved tree, which revealed that Oryza rufipogon was much more closely related to cultivated rice species than to the other two wild species. Oryza granulata was less closely related to either cultivated rice species or the other two wild species, and might be a unique and isolated taxon in the genus Oryza. The phylogenetic relationships of the three wild rice species and two cultivated rice subspecies inferred from ITS1 sequences is highly concordant with those based on the molecular evidence from isozyme, chloroplast DNA (cpDNA), mitochondrial DNA(mtDNA) and nuclear DNA (nDNA) of the genus Oryza .
基金Supported by the General Programs of the National Natural ScienceFoundation of China(30460019)Key Technologies R&D Program ofYunnan Province(2006NG34,2008C004Z)Cooperative Program Between Yunnan Academy of Agricultural Sciences (YAAS) and RDA of Korea~~
文摘[ Objective] The genetic diversity of the local cultivated aromatic rice and non-aromatic rice in Yunnan Province were compared to provide further genetic resources for breeding practice. [Method] Genetic diversity of 10 aromatic rice and 45 non-aromatic rice were analyzed by 64 SSR primers covered on 12 rice chromosomes. [ Result] Per locus 5.44 and 7.98 alleles in average were detected, ranging from 2 to 12 and from 2 to 17 in aromatic and non-aromatic rice, respectively. Average genetic multiplicity index（Hs） was 0.46 and 0.67 respectively. The average polymorphism information content （PIC） was 0.43 and 0.58 in aromatic and non-aromatic rice respectively. [ Conclusion] The results indicated that genetic diversity was higher in non-aromatic rice than in aromatic rice.
文摘Introduction The plants of genus Cistanche G. Beck, which belong to Orobanchaceae family, include about twenty species, and there are four species and one variety are distributed in the northwest part of China. The dried fleshy stem of Cistanche genus plant known as ＇Roucongrong＇ in Chinese traditional medicine, has long been used for kidney deficiency, female infertility, morbid leucorrhea, and neurasthenia. Studies on active components and pharmacological activities show that it possesses significant activities in enhancing potency, anti-fatigue, immuno-modulability, etc, and phenylethanoid glycosides are the major active components. Due to excessive exploitation, its natural resources are facing with exhaustion. In order to improve this situation, the plant has been cultivated on a large scale in the northwest part of China. Up to now, the chemical investigations of the cultivated Cistartcbe salsa have not been undertaken. In this paper we report seven phenylethanoid glycosides from the cultivated Cistanche salsa： echinacoside （1）, cistanoside A （2）, acteoside （3）, isoacteoside （4）, 2＇-acetylacteoside （5）, tubuloside B （6）, and eutigoside A （7）. Among them, compound 7 isolated fronl family Orobanchaceae was reported previously, and compounds 1, 2, 3, 4, 5 and 6 from the cultivated Cistanche salsa are reported for the first time.
基金Supported by the Early Special Program for the National "973" Key Basic Researches (2002CCC00800)~~
文摘[Objective] To study the chemic component of rice cell wall among different varieties and at different rice growth stages.[Method] The contents of neutral detergent fiber（NDF）,acid detergent fiber（ADF） and acid detergent lignin （ADL） of rice were analyzed by Van Soest Method.[Result] The contents of NDF,ADF and ADL varied from 55.63% to 71.70%,31.98% to 49.61% and 2.68% to 5.71% respectively,the highest content was gained in Dongxiang wide rice at dough stage,while the lowest content was obtained in rice heading stage of Xieqingzao B.In the same growth stage,the contents of NDF（except heading stage） and ADF were significantly（P0.05 or 0.01） difference among three varieties of rice,the highest content was Dongxiang wide rice,followed by Xiushui 110,and the lowest was Xieqingzao B.In the same variety rice,the content of NDF and ADF were significantly （P0.05 or 0.01） difference among three rice growth stages,the lowest contents was rice heading stage,followed by rice milk ripe stage,the highest was rice dough stage.[Conclusion] the NDF,ADF and other contents of indica rice were lower than that of ordinary wild rice and japonica rice,therefore,it owned a certain value in use of straw feed.If the indica rice could be harvest as earlier as possible,the rice straw together with silage fermentation and enzymatic hydrolysis could provide the effective diet for livestock.
基金National Natural Sci-ence Foundation of China (Grant No. 39900084) and KZCX1-10-07.
文摘Using the multi-temporal Landsat data and survey data of national resources, the authors studied the dynamics of cultivated land and landcover changes of typical ecological regions in China. The results of investigation showed that the whole distribution of the cultivated land shifted to Northeast and Northwest China, and as a result, the ecological quality of cultivated land dropped down. The seacoast and cultivated land in the area of Yellow River Mouth expanded by an increasing rate of 0.73 kma-1, with a depositing rate of 2.1 kma-1. The desertification area of the dynamic of Horqin Sandy Land increased from 60.02% of the total land area in1970s to 64.82% in1980s but decreased to 54.90% in early 1990s. As to the change of North Tibet lakes, the water area of the Namu Lake decreased by 38.58 km2 from year 1970 to 1988, with a decreasing rate of 2.14 km2a-1.
基金Supported by The Regional Sustainable Development of the Qing-TibetPlateau(2004)~~
文摘Using path analysis, correlation analysis, partial correlation analysis and system dynamics method to study the driving force of cultivated land in Qinghai Lake Area, and using gradually regression analysis to establish the driving force model of utilized change of cultivated land. Driving factors, action mechanism and process of utilized change of cultivated land were analyzed, and the differences during all factors were compared. The study provides some decision basis for sustainable utilization and management of land resources in Qinghai Lake Area.
基金Supported by National Key Basic Research Program of China(2007CB407203)Project of Teaching Research and Transformation in Hunan Agricultural University(B2010111)~~
文摘[Objective] This paper aimed at exploring the countermeasures for cultivated land protection and food security assurance and providing references for the formulation of policies related to cultivated land and grain production of the whole province.[Methods] Data of population,cultivated land,grain and others in Hunan Province during 1989 to 2008 were collected by county（district）.The changes of cultivated land and grain production of the whole province were analyzed using Geographical Information System（GIS）.[Results] The population was increasing continuously,the total area of cultivated land was reducing as a whole in spite of increasing in one or two year in one or two county,the total grain production and per capita grain yield was fluctuated within a certain range in recent 20 years of the whole province.The reducing of cultivated land was mainly due to the industrialization and urbanization development.The unbalanced quantity and quality of cultivated land,various natural disasters,readjustment of industry structure and inner readjustment in the agriculture,variety improvement and improvement of cultivated technology mainly affected of the grain production.[Conclusions] The protection of the quantity and quality of cultivated land has to be strengthened to ensure food security.It is important to enhance the ability of resisting drought and flood disasters by improving the construction of agriculture establishment in order to insure the productivity of cultivated land,to increase farmer’s enthusiasm for growing grain with favourable food policy,to improve the per unit area yield of grain by variety improvement and to increase grain sown area by enlarging the double-cropping rice sown area.
基金Supported by National Natural and Scientific Foundation Project(40761009)Yunnan Province Applied Fundamental Research Project(2007D081M)Assisted by the Project of Yunnan Province Department of Education(06Y045A)~~
文摘Based upon ArCGIS,the vertical spatial transfer law of cultivated land in Yunnan was studied through referring to three periods of remote sensing data of Land-Use and Land-Cover Change（LUCC） in Yunnan in 1986,1995 and 2000,Digital Elevation Model（DEM） of Yunnan Province and LUCC data in Yunnan from 2000 to 2008.The main factors that promote vertical spatial transfer law of cultivated land were analyzed by using SPSS,and the influence of policies and government acts upon the change of cultivated land was clarified.The findings indicated that for Yunnan cultivated land there is a trend that in the vertical direction steep slope areas and gentle slope areas divert into mid-slope areas.The primary factor influencing the vertical spatial transfer of cultivated land was policies and government acts.Also,the gradient of ground,total population and number of agriculture employed persons were also main factors in influencing the change of cultivated land,whose influence was obviously below policies and government acts.
基金Supported by National Science and Technology Support Projects(2007BAD64B01)~~
文摘[Objective]The aim was to analyze genetic diversity of SSR markers in Hordeum vulgare L.in Qinghai Province and lay a foundation for screening and protecting some excellent H.vulgare cultivars.[Method]SSR markers were used to evaluate the genetic diversity of 42 cultivated H.vulgare from Qinghai Province.[Result]42 H.vulgare showed polymorphism in 7 SSR markers locus.A total of 24 alleles were identified,and the number of alleles per locus ranged from 1 to 6,with an average of 3.0.According to SSR markers polymorphism,42 H.vulgare could be divided into 4 groups,namely I,II,III and IV.[Result]The study indicated that cultivated H.vulgare from Qinghai Province is rich in genetic diversity,which will provide reference for selecting parent of H.vulgare breeding.
基金Under the auspices of Key Project of Science and Technology of the Ministry of Education (No. 03111)IncubationFund Project of Science and Technology Committee of Chongqing (No. 017079)
文摘The conflicts among food security, economic development and ecological protection are the “sticking point” of undeveloped southwestern mountainous areas of China. The objectives of this study are to identify appropriate inte- grated indicators influencing the classification and gradation of cultivated land quality in the southwestern mountainous area of China based on semi-structure interview, and to promote the monitoring of cultivated land quality in this region. Taking Bishan County of Chongqing as a study case, the integrated indicators involve the productivity, protection, ac- ceptability, and stability of cultivated land. The integrated indicators accord with the characteristics of land resources and human preference in southwestern mountainous area of China. In different agricultural zones, we emphasize different indicators, such as emphasizing productivity, stabilization and acceptability in low hilly and plain agricultural integrative zone (LHP-AIZ), protection, productivity and stability in low mountain and hill agro-forestry ecological zone (LMH-AEZ), and acceptability in plain outskirts integrative agricultural zone (PO-IAZ), respectively. The pronounced difference of classification and gradation of cultivated land, regardless of inter-region or intra-region, is observed, with the reducible rank from PO-IAZ, LHP-AIZ to LMH-AEZ. Research results accord with the characteristics of assets management and intensive utilization of cultivated land resources in the southwestern mountainous area of China. Semi-structure interview adequately presents the principal agent of farmers in agricultural land use and rural land market. This method is very effective and feasible to obtain data of the quality of cultivated land in the southwestern mountainous area of China.
