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Optimal motion cueing algorithm for accelerating phase of manned spacecraft in human centrifuge 被引量:2
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作者 Ali MAHMOODI Isooda KAZEMI 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2020年第7期1991-2001,共11页
This paper presents a novel optimal Motion Cueing Algorithm(MCA)to control the rotations of a Human Centrifuge(HC)and achieve the best simulation of a Space Craft(SC)motion.Relations of the specific forces sensed by a... This paper presents a novel optimal Motion Cueing Algorithm(MCA)to control the rotations of a Human Centrifuge(HC)and achieve the best simulation of a Space Craft(SC)motion.Relations of the specific forces sensed by astronauts of the SC and the HC have been derived and linearized.A Linear Quadratic Regulator(LQR)controller is implemented for the problem which tends to minimize the error between the two sensed specific forces as well as control input in a cost function.It results in control inputs of the HC to generate its sensed specific force as close as possible to the one in the SC.The algorithm is implemented for both linearized and nonlinear portions of a US space shuttle mission trajectory as a verification using MATLAB.In longitudinal direction,the proposed MCA,works well when the acceleration is less than 2 g in which the tracking error does not exceed 12%.In lateral direction the tracking is much better even in nonlinear region since the error remains less than 7%for tilting up to 50°.Finally,the effect of weight matrixes in the LQR cost function on overall weight and power of the HC motion system is discussed. 展开更多
关键词 Accelerating phase Human centrifuge Manned spacecraft Optimal motion cueing Specific force
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A Novel Nonlinear Scaling Method for Optimal Motion Cueing Algorithm in Flight Simulator 被引量:2
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作者 ZHU Daoyang DUAN Shaoli +1 位作者 SHANG Jinqiu GUO Ping 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2020年第5期452-460,共9页
Motion cueing algorithm plays a key role in simulator motion reproduction and improves the realism of movement sensation by combining with the human vestibular system.It is well established that scaling&limiting s... Motion cueing algorithm plays a key role in simulator motion reproduction and improves the realism of movement sensation by combining with the human vestibular system.It is well established that scaling&limiting should be used to decrease the amplitude of the acceleration and angular velocity signals for making full use of limited workspace of motion platform.A novel nonlinear scaling method based on a third-order polynomial and back propagation(BP)neural networks for the motion cueing algorithm is proposed in this paper.The third-order polynomial method is applied to the low amplitude segment of the input signal to minimize the trigger delay of the sensation acceleration;in the high amplitude segment,the BP neural network is used to adaptively adjust the scaling factor of the input signal,to avoid washout displacement and angular displacement beyond the boundary of the workspace.The simulation experiment is verified in the longitudinal/pitch direction for flight simulator,and the result implies that the proposed method not only can overcome the problem of constant scaling parameter and improve motion platform workspace utilization,but also reduce the false cues during the motion simulation process. 展开更多
关键词 optimal motion cueing algorithm nonlinear scaling human vestibular system dynamic fidelity
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A Design of Nonlinear Scaling and Nonlinear Optimal Motion Cueing Algorithm for Pilot’s Station
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作者 ZHU Daoyang YAN Jun DUAN Shaoli 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2021年第6期513-520,共8页
Motion cueing algorithms(MCA)are often applied in the motion simulators.In this paper,a nonlinear optimal MCA,taking into account translational and rotational motions of a simulator within its physical limitation,is d... Motion cueing algorithms(MCA)are often applied in the motion simulators.In this paper,a nonlinear optimal MCA,taking into account translational and rotational motions of a simulator within its physical limitation,is designed for the motion platform aiming to minimize human’s perception error in order to provide a high degree of fidelity.Indeed,the movement sensation center of most MCA is placed at the center of the upper platform,which may cause a certain error.Pilot’s station should be paid full attention to in the MCA.Apart from this,the scaling and limiting module plays an important role in optimizing the motion platform workspace and reducing false cues during motion reproduction.It should be used along within the washout filter to decrease the amplitude of the translational and rotational motion signals uniformly across all frequencies through the MCA.A nonlinear scaling method is designed to accurately duplicate motions with high realistic behavior and use the platform more efficiently without violating its physical limitations.The simulation experiment is verified in the longitudinal/pitch direction for motion simulator.The result implies that the proposed method can not only overcome the problem of the workspace limitations in the simulator motion reproduction and improve the realism of movement sensation,but also reduce the false cues to improve dynamic fidelity during the motion simulation process. 展开更多
关键词 nonlinear optimal motion cueing algorithm(MCA) nonlinear scaling and limiting pilot’s station dynamic fidelity
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Development of Cueing Algorithm Based on “Closed-Loop” Control for Flight Simulator Motion System 被引量:3
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作者 ZHU Daoyang DUAN Shaoli FANG Da 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2019年第5期376-382,共7页
The classical washout algorithm had fixed gains and manually constructed filters, so that it led to poor adaptability. Furthermore, it lost the sustained acceleration cues of high-and mid-frequency in cross-over(tilt-... The classical washout algorithm had fixed gains and manually constructed filters, so that it led to poor adaptability. Furthermore, it lost the sustained acceleration cues of high-and mid-frequency in cross-over(tilt-coordination) channel, and the acceleration of cross-over frequency was also limited by angular velocity limiter, so the false cues in flight simulation process were clearly perceived by pilots. The paper studied the characteristics of the classical washout algorithm and flight simulator motion platform, tried to redesign the source of cross-over acceleration channel and translation acceleration channel, and transferred the part of cross-over acceleration that was unsimulated sustained acceleration to translation acceleration channel. Comparisons were mainly made between classical washout algorithm and revised algorithm in a longitudinal/pitch direction. The evaluation was based on the implementation of human vestibular perception system. The results demonstrated that the revised algorithm could significantly reduce the phase lag, and improved the spikes tracking performance. Furthermore, sensory angular velocity and the error of sensory acceleration were strictly controlled within the threshold of human perception system, and the displacement was a little broader than the classical washout algorithm. Therefore, it was proved that the new algorithm could diminish the filters parameters and heighten the self-adaptability for the washout algorithm. In addition, the magnitude of false cues was remarkably reduced during flight simulator, and the workspace utilization of the motion platform was developed by "closed-loop" control system. 展开更多
关键词 classical WASHOUT ALGORITHM human VESTIBULAR system "closed-loop" control false CUES
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Model Predictive Control based Motion Cueing Algorithm for Driving Simulator
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作者 Ayesha Hameed Ali Soltani Sharif Abadi Andrzej Ordys 《Journal of Systems Science and Systems Engineering》 SCIE EI CSCD 2024年第5期607-626,共20页
Thanks to the emerging integration of algorithms and simulators, recent Driving Simulators (DS) find enormous potential in applications like advanced driver-assistance devices, analysis of driver’s behaviours, resear... Thanks to the emerging integration of algorithms and simulators, recent Driving Simulators (DS) find enormous potential in applications like advanced driver-assistance devices, analysis of driver’s behaviours, research and development of new vehicles and even for entertainment purposes. Driving simulators have been developed to reduce the cost of field studies, allow more flexible control over circumstances and measurements, and safely present hazardous conditions. The major challenge in a driving simulator is to reproduce realistic motions within hardware constraints. Motion Cueing Algorithm (MCA) guarantees a realistic motion perception in the simulator. However, the complex nature of the human perception system makes MCA implementation challenging. The present research aims to improve the performance of driving simulators by proposing and implementing the MCA algorithm as a control problem. The approach is realized using an actual vehicle model integrated with a detailed model of the human vestibular system, which accurately reproduces the driver’s perception. These perception motion signals are compared with simulated ones. A 2-DOF stabilized platform model is used to test the results from the two proposed control strategies, Proportional Integrator and Derivative (PID) and Model Predictive Control (MPC). 展开更多
关键词 Driving simulator motion cueing algorithm control theory model predictive control PID
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鄱阳湖洪泛湿地植被碳利用率时空格局演变及其对干旱的响应
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作者 袁灿煜 李相虎 +4 位作者 张丹 蔺亚玲 谭志强 叶许春 孙童 《水资源与水工程学报》 北大核心 2025年第3期89-98,共10页
为阐明湿地生态系统的碳循环过程、精确评估区域碳收支状况以及生态系统服务功能的稳定性,基于多源遥感影像融合生成的长时序归一化植被指数(NDVI)数据集,并结合CASA(Carnegie-Ames-Stanford approach)模型和光能利用率集成(LUE)模型解... 为阐明湿地生态系统的碳循环过程、精确评估区域碳收支状况以及生态系统服务功能的稳定性,基于多源遥感影像融合生成的长时序归一化植被指数(NDVI)数据集,并结合CASA(Carnegie-Ames-Stanford approach)模型和光能利用率集成(LUE)模型解析了1986-2020年间鄱阳湖洪泛湿地植被碳利用率(CUE)的时空格局演变及其对干旱的响应特征。结果表明:在过去35 a间,鄱阳湖洪泛湿地植被碳利用率平均降低了44.08%,且碳利用率减小的区域面积覆盖了全湖区的63.99%;植被碳利用率的空间分别呈现出西南湖区>东部湖区>北部湖区的趋势,而在季节变化中表现为夏季>秋季>春季>冬季;芦荻、蒌蒿群落及林地的碳利用率整体保持在较高水平,平均为0.35~0.44;相较于正常年份,干旱年份中鄱阳湖洪泛湿地有高达69.11%的区域其植被碳利用率出现下降,尤其是在夏季,所有湿地植被类型的碳利用率均有所减少,其中苔草和蓼子草-虉草群落的平均降幅为0.16;春季和冬季期间,多数湿地植被类型的碳利用率在干旱年份略有增加,特别是蓼子草-虉草群落和林地表现得最为明显。 展开更多
关键词 碳利用率(CUE) 时空格局 干旱效应 遥感数据融合 CASA模型 光能利用率集成模型 洪泛湿地 鄱阳湖
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Biomaterial‑based mechanical regulation facilitates scarless wound healing with functional skin appendage regeneration 被引量:1
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作者 Ying-Ying Li Shuai-Fei Ji +2 位作者 Xiao-Bing Fu Yu-Feng Jiang Xiao-Yan Sun 《Military Medical Research》 2025年第1期96-120,共25页
Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages,ultimately impairing its normal physiological function.A... Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages,ultimately impairing its normal physiological function.Accumulating evidence underscores the potential of targeted modulation of mechanical cues to enhance skin regeneration,promoting scarless repair by influencing the extracellular microenvironment and driving the phenotypic transitions.The field of skin repair and skin appendage regeneration has witnessed remarkable advancements in the utilization of biomaterials with distinct physical properties.However,a comprehensive understanding of the underlying mechanisms remains somewhat elusive,limiting the broader application of these innovations.In this review,we present two promising biomaterial-based mechanical approaches aimed at bolstering the regenerative capacity of compromised skin.The first approach involves leveraging biomaterials with specific biophysical properties to create an optimal scarless environment that supports cellular activities essential for regeneration.The second approach centers on harnessing mechanical forces exerted by biomaterials to enhance cellular plasticity,facilitating efficient cellular reprogramming and,consequently,promoting the regeneration of skin appendages.In summary,the manipulation of mechanical cues using biomaterial-based strategies holds significant promise as a supplementary approach for achieving scarless wound healing,coupled with the restoration of multiple skin appendage functions. 展开更多
关键词 SCARLESS Wound healing Biomaterials Mechanical cues Skin appendages
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Effect of external and internal cues on core muscle activation during the Sahrmann five-level core stability test
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作者 Skyla Stifter Jessie McCaffrey +2 位作者 Tyler Nichols Ayse Ozcan Edeer Justine Ward 《World Journal of Methodology》 2025年第1期95-104,共10页
BACKGROUND Pain in the back or pelvis or fear of back pain may affect the timing or cocontraction of the core muscles.In both static and dynamic movements,the Sahrmann core stability test provides an assessment of cor... BACKGROUND Pain in the back or pelvis or fear of back pain may affect the timing or cocontraction of the core muscles.In both static and dynamic movements,the Sahrmann core stability test provides an assessment of core muscle activation and a person's ability to stabilize the lumbopelvic complex.Preparatory cues and images can be used to increase the activation of these muscles.To attain optimal movement patterns,it will be necessary to determine what cueing will give the most effective results for core stability.AIM To investigate the effects of external and internal cues on core muscle activation during the Sahrmann five-level core stability test.METHODS Total 68 participants(21.83±3.47 years)were randomly allocated to an external(n=35)or internal cue group(n=33).Participants performed the Sahrmann fivelevel core stability test without a cue as baseline and the five-level stability exercises with an internal or external cue.External cue group received a pressure biofeedback unit(PBU),and the internal cue group received an audio cue.A Delsys Trigno^(TM)surface electromyography unit was used for muscle activation from the rectus abdominis,external oblique,and transverse abdominis/internal oblique muscles.RESULTS Linear mixed effects model analysis showed that cueing had a significant effect on core muscle activation(P=0.001);however,there was no significant difference between cue types(internal or external)(P=0.130).CONCLUSION Both external and internal cueing have significant effects on core muscle activation during the Sahrmann five-level core stability test and the PBU does not create higher muscle activation than internal cueing. 展开更多
关键词 External cue Internal cue Muscle activation Core muscles Lumbopelvic stability Pressure biofeedback unit
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Robust human motion prediction via integration of spatial and temporal cues
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作者 ZHANG Shaobo LIU Sheng +1 位作者 GAO Fei FENG Yuan 《Optoelectronics Letters》 2025年第8期499-506,共8页
Research on human motion prediction has made significant progress due to its importance in the development of various artificial intelligence applications.However,effectively capturing spatio-temporal features for smo... Research on human motion prediction has made significant progress due to its importance in the development of various artificial intelligence applications.However,effectively capturing spatio-temporal features for smoother and more precise human motion prediction remains a challenge.To address these issues,a robust human motion prediction method via integration of spatial and temporal cues(RISTC)has been proposed.This method captures sufficient spatio-temporal correlation of the observable sequence of human poses by utilizing the spatio-temporal mixed feature extractor(MFE).In multi-layer MFEs,the channel-graph united attention blocks extract the augmented spatial features of the human poses in the channel and spatial dimension.Additionally,multi-scale temporal blocks have been designed to effectively capture complicated and highly dynamic temporal information.Our experiments on the Human3.6M and Carnegie Mellon University motion capture(CMU Mocap)datasets show that the proposed network yields higher prediction accuracy than the state-of-the-art methods. 展开更多
关键词 human p integration spatial temporal cues ristc human motion prediction temporal cues mixed feature extractor spatial cues artificial intelligence spatio temporal correlation
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The relationship between proxy decision-making content and cues by families of patients with malignant brain tumor: A descriptive qualitative study
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作者 Runa Tokunaga Fumiyo Ishikawa 《International Journal of Nursing Sciences》 2025年第2期169-175,共7页
Objectives This study aimed to clarify the relationship between the content of proxy decision-making made by families of patients with malignant brain tumors regarding treatment policies and daily care and the cues le... Objectives This study aimed to clarify the relationship between the content of proxy decision-making made by families of patients with malignant brain tumors regarding treatment policies and daily care and the cues leading to those decisions.Methods Semi-structured personal interviews were used to collect data.Seven family members of patients with malignant brain tumors were selected to participate in the study by purposive sampling method from June to August 2022 in the Patient Family Association of Japan.Responses were content analyzed to explore the relationship between the content of decisions regarding“treatment policies”and“daily care”and the cues influencing those decisions.Semi-structured interviews were analyzed by using thematic analysis.Results The contents of proxy decisions regarding“treatment policies”included implementation,interruption,and termination of initial treatments,free medical treatments,use of respirators,and end-of-life sedation and included six cues:treatment policies suggested by the primary physician,information and knowledge about the disease and treatment obtained by the family from limited resources,perceived life threat from symptom worsening,words and reactions from the patient regarding treatment,patient’s personality and way of life inferred from their treatment preferences,family’s thoughts and values hoping for better treatment for the patient.Decisions for“daily care”included meal content and methods,excretion,mobility,maintaining cleanliness,rehabilitation,continuation or resignation from work,treatment settings(outpatient or inpatient),and ways to spend time outside and included seven cues:words and thoughts from the patient about their way of life,patient’s reactions and life history inferred from their preferred way of living,things the patient can do to maintain daily life and roles,awareness of the increasing inability to do things in daily life,family’s underlying thoughts and values about how to spend the remaining time,approval from family members regarding the care setting,advice from medical professionals on living at home.Conclusions For“treatment policies,”guidelines from medical professionals were a key cue,while for“daily care,”the small signs from the patients in their daily lives served as cues for proxy decision-making.This may be due to the lack of information available to families and the limited time available for discussion with the patient.Families of patients with malignant brain tumors repeatedly use multiple cues to make proxy decision-making under high uncertainty.Therefore,nurses supporting proxy decision-making should assess the family’s situation and provide cues that facilitate informed and confident decisions. 展开更多
关键词 Brain neoplasms FAMILY Nurses CUES Proxy decision-making
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Antipredator decisions of male Trinidadian guppies(Poecilia reticulata) depend on social cues from females
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作者 Alix J.P.Brusseau Laurence E.A.Feyten +3 位作者 Adam L.Crane Indar W.Ramnarine Maud C.O.Ferrari Grant E.Brown 《Current Zoology》 2025年第2期205-211,共7页
Many prey species rely on publicly available personal and social information regarding local predation threats to assess risks and make contextappropriate behavioral decisions.However,in sexually dimorphic species,mal... Many prey species rely on publicly available personal and social information regarding local predation threats to assess risks and make contextappropriate behavioral decisions.However,in sexually dimorphic species,males and females are expected to differ in the perceived costs and/orbenefts associated with predator avoidance decisions.Recent studies suggest that male Trinidadian guppies(Poecilia reticulata)show reducedor absent responses to acute personal information cues,placing them at greater risk of predation relative to females.Our goal here was totest the hypothesis that adult(reproductively active)male guppies rely on social information to limit potential costs associated with their lack ofresponse to risky personal cues.Adult male guppies were exposed to personal chemosensory cues(either conspecifc alarm cues(AC),a novelodor,or a water control)in the presence of a shoal of three females inside a holding container that allowed the transmission of visual but notchemical cues.At the same time,we exposed females to either risk from AC or no risk,resulting in the display of a range of female behavior,from calm to alarmed,available as social information for males.Alarmed females caused male fright activity to increase and male interest infemales to decrease,regardless of the personal cue treatment.These results indicate that male guppies rely more on female information regarding predation risk than their own personal information,probably to balance trade-offs between reproduction and predator avoidance. 展开更多
关键词 alarm cues information sources predation risk sex differences trade-off
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Reflections on dengue vaccination:Parallels,challenges,and lessons from Indonesia and the Philippines experiences
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作者 Rowalt Alibudbud 《Asian Pacific Journal of Tropical Medicine》 2025年第11期521-524,共4页
To the Editor:Salam et al.found that more than half(54.2%)of Indonesian parents surveyed were willing to pay for a dengue vaccination[1].Using the Health Belief Model,the study identified four constructs that signific... To the Editor:Salam et al.found that more than half(54.2%)of Indonesian parents surveyed were willing to pay for a dengue vaccination[1].Using the Health Belief Model,the study identified four constructs that significantly influenced willingness to pay,including Perceived Susceptibility,Perceived Severity,Perceived Benefits,and Cues to Action[1].Interestingly,Perceived Barriers,including cost and potential side effects,did not show a significant effect[1]. 展开更多
关键词 health belief modelthe Willingness pay Health belief model Dengue vaccination cues action interestinglyperceived perceived susceptibilityperceived Perceived susceptibility dengue vaccination using
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Spatio-Temporal Analysis of Vegetation Cover in Char Fasson and Galachipa Upazila of Bangladesh (1994-2024) Using Landsat Imagery
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作者 Khaled Jubair Shabab MD. Mahmudul Hasan Shahed +2 位作者 Maria Binta Malek MD. Ashraful Habib Md Shahedur Rashid 《Journal of Geographic Information System》 2025年第1期66-79,共14页
This research aims to analyse the spatio-temporal changes of vegetation cover in coastal regions of Char Fasson and Galachipa Upazila, Bangladesh for a period of 30 years (1994-2024) based on Landsat satellite imagery... This research aims to analyse the spatio-temporal changes of vegetation cover in coastal regions of Char Fasson and Galachipa Upazila, Bangladesh for a period of 30 years (1994-2024) based on Landsat satellite imagery and NDVI. Through the evaluation of NDVI this paper classifies vegetation as no water/bare vegetation, slightly densed vegetation, moderately densed vegetation, and highly densed vegetation. The findings reveal significant fluctuations in vegetation cover: from 1994 to 2004, there has been an increase in vegetation density implying that afforestation has created more moderate and highly densed vegetation out of density vegetation. However, between 2004 and 2014, vegetation cover decreased because some cyclones, like Sidr and Aila, affected the coastal forest of Bangladesh. Other attempts to afforestation supported improved coverage from vegetation between 2014 and 2024. These findings provide clear evidence of the sustainable benefits of coastal afforestation in the reduction of coastal erosion and storm surges that affect vegetation and coasts. Knowledge gained in this research is highly useful to the environmental planners on recommendations for sustainable land uses and preservation to build up ecological stability in Bangladesh weak coastal areas. 展开更多
关键词 NDVI (Normalized Difference Vegetation Index) Remote Sensing in Vegetation Monitoring Delta Cue Technique Coastal Management Cyclone Impact on Vegetation
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CUE结构域蛋白2对高糖诱导的肾小管上皮细胞损伤的影响研究 被引量:9
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作者 张鹤 周蕾 +1 位作者 许静 房辉 《中国糖尿病杂志》 CAS CSCD 北大核心 2020年第10期774-778,共5页
目的探讨CUE结构域蛋白2(CUEDC2)对高糖诱导的肾小管上皮细胞损伤的影响。方法肾小管上皮细胞HK-2分为高糖处理组(HG)、转染对照载体后给予高糖处理组(NC+HG)、转染过表达CUEDC2载体后给予高糖处理组(CUEDC2+HG)及对照组(Con)。流式细... 目的探讨CUE结构域蛋白2(CUEDC2)对高糖诱导的肾小管上皮细胞损伤的影响。方法肾小管上皮细胞HK-2分为高糖处理组(HG)、转染对照载体后给予高糖处理组(NC+HG)、转染过表达CUEDC2载体后给予高糖处理组(CUEDC2+HG)及对照组(Con)。流式细胞仪测定细胞凋亡;试剂盒检测细胞中丙二醛(MDA)、超氧化物歧化酶(SOD)水平和培养液中TNF-α、IL-8水平。结果与Con组比较,HG组细胞凋亡率、MDA、TNF-α及IL-8升高[(6.23±0.65)%vs(18.56±3.14)%,(0.06±0.01)vs(0.15±0.02)nmol/mg,(29.54±3.56)vs(56.10±6.30)mg/L,(10.42±1.23)vs(19.59±1.68)mg/L;P<0.05],SOD活性降低[(36.20±2.78)vs(20.17±1.45)U/mg,P<0.05];与NC+HG、HG组比较,CUEDC2+HG组细胞凋亡率、MDA、TNF-α及IL-8降低[(17.14±2.98)%vs(18.56±3.14)%vs(11.47±1.20)%,(0.14±0.01)vs(0.15±0.02)vs(0.10±0.02)nmol/mg,(55.49±5.97)vs(56.10±6.30)vs(41.20±3.75)mg/L,(19.59±1.68)vs(20.12±2.35)vs(14.65±1.42)mg/L,P<0.05],SOD活性升高[(20.17±1.45)vs(19.64±1.37)vs(25.96±3.26)U/mg,P<0.05]。结论过表达CUEDC2可减少高糖诱导的肾小管上皮细胞炎症因子释放,降低细胞氧化应激,减少细胞凋亡,保护高糖诱导损伤的肾小管上皮细胞。 展开更多
关键词 CUE结构域蛋白2 高糖 肾小管上皮细胞 细胞凋亡 氧化应激
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CUEDC2通过上皮-间质转化促进人乳腺癌MCF-7细胞侵袭转移 被引量:6
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作者 李彦姝 汤丽娜 +2 位作者 张红艳 高云凌 李丰 《现代肿瘤医学》 CAS 2016年第11期1681-1683,共3页
目的:探讨CUE结构域2(CUE domain-containing 2,CUEDC2)蛋白对人乳腺肿瘤细胞侵袭转移的作用机制。方法:人乳腺肿瘤细胞MCF-7转染的Myc-CUEDC2真核表达载体,提取转染细胞的总蛋白和总RNA进行蛋白免疫印迹和实时定量PCR检测上皮-间质转化... 目的:探讨CUE结构域2(CUE domain-containing 2,CUEDC2)蛋白对人乳腺肿瘤细胞侵袭转移的作用机制。方法:人乳腺肿瘤细胞MCF-7转染的Myc-CUEDC2真核表达载体,提取转染细胞的总蛋白和总RNA进行蛋白免疫印迹和实时定量PCR检测上皮-间质转化(EMT)标志物上皮-钙黏蛋白(E-cadherin)和转录因子SLUG的表达。结果:过表达CUEDC2蛋白能够下调E-cadherin蛋白和基因的表达,而上调转录因子SLUG基因和蛋白的表达。结论:CUEDC2可能参与调节乳腺肿瘤细胞发生上皮-间质转化,促进乳腺肿瘤侵袭转移的发生,CUEDC2可能是人乳腺肿瘤侵袭转移的治疗靶点。 展开更多
关键词 乳腺肿瘤 CUE结构域2 上皮-间质转化 侵袭转移
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CUEDC2在浆液性卵巢癌组织中的表达及其与临床指标的相关性分析 被引量:3
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作者 胡凌云 宁静 +2 位作者 李爱玲 李亚里 游艳琴 《解放军医学杂志》 CAS CSCD 北大核心 2020年第12期1265-1269,共5页
目的观察CUE结构域蛋白2(CUEDC2)在浆液性卵巢癌组织中的表达,并分析其与相关临床指标的关系。方法采用免疫组化法检测75例浆液性卵巢癌组织及20例未发生转移的输卵管伞端组织中CUEDC2蛋白的表达情况,并分析CUEDC2阳性表达与雌激素受体(... 目的观察CUE结构域蛋白2(CUEDC2)在浆液性卵巢癌组织中的表达,并分析其与相关临床指标的关系。方法采用免疫组化法检测75例浆液性卵巢癌组织及20例未发生转移的输卵管伞端组织中CUEDC2蛋白的表达情况,并分析CUEDC2阳性表达与雌激素受体(ER)、孕激素受体(PR)表达的相关性。结果75例浆液性卵巢癌患者中,FIGO分期:Ⅰ期患者的CUEDC2评分为1.35±0.60,Ⅱ期为1.54±0.57,Ⅲ期为1.78±0.71,Ⅳ期为2.13±0.27,各组间比较差异均有统计学意义(P=0.04),CUEDC2评分随FIGO分期的增加而升高,即CUEDC2的表达量逐级递增。早期患者的CUEDC2评分明显低于晚期患者(P=0.04)。按病理分级统计,低、中、高分化3组间评分差异无统计学意义。CUEDC2表达与卵巢癌患者的年龄、病理分级、有无淋巴结转移无关,与手术分期有关(P=0.014)。CUEDC2的表达与ER、PR的表达均无相关性(r=–0.0785、r=0.0111)。CUEDC2阳性患者的中位无病生存期(DFS)为32.6个月,中位总生存期(OS)为54.3个月,而阴性患者的中位DFS为51.9个月,中位OS为63.5个月,CUEDC2阳性组及阴性组的OS无明显差异(P>0.05),而CUEDC2阳性组的DFS短于阴性组(P<0.05)。结论CUEDC2在浆液性卵巢癌组织中呈高表达,且与患者的肿瘤分期、无病生存期相关,与ER、PR表达无相关性。 展开更多
关键词 CUE结构域蛋白2 卵巢肿瘤 免疫组织化学
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CUE结构域2表达与结直肠癌淋巴结转移的关系探讨 被引量:4
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作者 胡时栋 胡子龙 +9 位作者 邹贵军 吴有军 邢晓伟 李宇轩 张凯 何长征 王迪 杨宇 王玉锋 杜晓辉 《解放军医学院学报》 CAS 2017年第5期399-401,共3页
目的探讨CUE结构域2(CUE domain-containing 2,CUEDC2)表达与结直肠癌淋巴结转移的关系。方法收集2015年12月-2016年5月在我院住院手术的153例结直肠癌患者的临床资料与癌组织蜡块,使用免疫组化方法分析CUEDC2的表达,对CUEDC2与结直肠... 目的探讨CUE结构域2(CUE domain-containing 2,CUEDC2)表达与结直肠癌淋巴结转移的关系。方法收集2015年12月-2016年5月在我院住院手术的153例结直肠癌患者的临床资料与癌组织蜡块,使用免疫组化方法分析CUEDC2的表达,对CUEDC2与结直肠癌淋巴结转移的关系进行单因素及多因素分析。结果结直肠癌淋巴结转移患者CUEDC2免疫组化评分明显高于无淋巴结转移患者(7.41±1.46 vs 4.61±1.27,P<0.05)。多元Logistic回归分析结果表明,高CUEDC2免疫组化评分是结直肠癌发生淋巴结转移的独立危险因素。结论 CUEDC2免疫组化评分≥8分与结直肠癌淋巴结转移相关,CUEDC2可能对结直肠癌淋巴结转移起到促进作用。 展开更多
关键词 CUE结构域2 结直肠癌 淋巴结转移
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同期发情技术在安格斯牛繁殖中的应用实验研究 被引量:7
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作者 张晓红 辛博成 +3 位作者 陈俐静 郑小风 刘英玉 姚刚 《草食家畜》 2018年第5期7-10,共4页
为了提高博乐某繁育场安格斯母牛受胎率,繁育场采用国内较先进的阴道埋栓技术[1]及用孕激素(戈那瑞林(GnRH))与前列腺激素(氯前列醇钠(PG))结合处理的同期发情技术[2-3],历时两个月分两批对984头母牛进行同期发情处理。其中阴道埋栓分... 为了提高博乐某繁育场安格斯母牛受胎率,繁育场采用国内较先进的阴道埋栓技术[1]及用孕激素(戈那瑞林(GnRH))与前列腺激素(氯前列醇钠(PG))结合处理的同期发情技术[2-3],历时两个月分两批对984头母牛进行同期发情处理。其中阴道埋栓分为两种[4],第一种埋栓(CIDR)144头,第二种埋栓(CUEMATE)埋栓175头,合计319头。在二月初,由专业技术人员利用B超技术对984头牛进行孕检,其中怀孕母牛366头,怀孕率达到38%,达到预期标准。通过这次同期发情处理,使得该场母牛受胎率提高了不少,节约了成本,并且为了更科学的使用阴道栓,对比了CIDR与CUE-MATE在使用相同同期发情方案的受胎率[5]。 展开更多
关键词 安格斯牛 同期发情 戈那瑞林(GnRH) 氯前列醇钠(PG) 牛用孕酮阴道栓(CIDR、CUE—MATE)
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子宫内膜癌组织中SOCS-1、CUEDC2的表达变化及意义 被引量:3
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作者 戴如星 高彩云 +2 位作者 杨俊 郑雪群 明芳 《山东医药》 CAS 2021年第7期65-68,共4页
目的探讨子宫内膜癌组织中细胞因子信号传导抑制因子(SOCS1)、CUE结构域蛋白2(CUEDC2)的表达变化及意义。方法选择80例子宫内膜癌患者的临床资料,应用免疫组化法检测癌组织及癌旁组织中SOCS1及CUEDC2表达。统计学分析癌组织中SOCS1、CUE... 目的探讨子宫内膜癌组织中细胞因子信号传导抑制因子(SOCS1)、CUE结构域蛋白2(CUEDC2)的表达变化及意义。方法选择80例子宫内膜癌患者的临床资料,应用免疫组化法检测癌组织及癌旁组织中SOCS1及CUEDC2表达。统计学分析癌组织中SOCS1、CUEDC2表达与患者临床病理参数关系。Spearman秩相关分析SOCS1与CUEDC2表达的相关性。结果与癌旁组织相比,子宫内膜癌组织中SOCS1表达阳性率降低,而CUEDC2表达阳性率升高(P<0.05)。子宫内膜癌组织中SOCS1、CUEDC2表达均与肿瘤分期、淋巴结转移相关(P均<0.05),而与年龄、肿瘤直径、肌层浸润及分化程度无关(P均>0.05)。子宫内膜癌组织中SOCS1与CUEDC2的表达呈负相关(r=-0.598,P<0.05)。结论子宫内膜癌组织中SOCS1表达下调,CUEDC2表达上调,二者表达与肿瘤分期和淋巴结转移有关;检测二者表达有助于子宫内膜癌病情判断。 展开更多
关键词 子宫内膜癌 细胞因子信号传导抑制因子 CUE结构域蛋白2
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鼻咽癌CNE2与放疗抗拒株CNE2R细胞的放化疗敏感性及其CUEDC2表达量比较 被引量:2
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作者 许玲玉 程金建 +1 位作者 朱玲 杨华 《广西医学》 CAS 2017年第3期376-381,共6页
目的比较鼻咽癌CNE2细胞与其放疗抗拒株CNE2R细胞的放化疗敏感性及两者CUE结构域2(CUEDC2)蛋白表达量的差异。方法裸鼠成瘤法比较CNE2与CNE2R细胞的体内生长情况;四甲基偶氮唑蓝(MTT)法检测CNE2及CNE2R细胞对顺铂、多西紫杉醇的敏感性;... 目的比较鼻咽癌CNE2细胞与其放疗抗拒株CNE2R细胞的放化疗敏感性及两者CUE结构域2(CUEDC2)蛋白表达量的差异。方法裸鼠成瘤法比较CNE2与CNE2R细胞的体内生长情况;四甲基偶氮唑蓝(MTT)法检测CNE2及CNE2R细胞对顺铂、多西紫杉醇的敏感性;流式细胞术检测细胞周期及细胞凋亡;实时聚合酶链反应(RT-PCR)检测CUEDC2的相对mRNA水平、蛋白质免疫印迹法检测CUEDC2蛋白的表达量。结果 CNE2生长速度快于CNE2R,对多西紫杉醇更敏感(P<0.05)。0 Gy、4 Gy、8 Gy照射剂量下,CNE2R的G2/M期阻滞较CNE2明显,但12 Gy照射剂量下,CNE2R的G2/M期阻滞不如CNE2明显(均P<0.05)。在8 Gy、12 Gy照射剂量下,CNE2的凋亡率高于CNE2R(P<0.05)。CNE2R细胞CUEDC2蛋白相对mRNA扩增水平和蛋白表达量均高于CNE2(P<0.05)。结论放疗抗拒株细胞CNE2R的放化疗敏感性较其亲代细胞CNE2低,CUEDC2蛋白的表达量较CNE2的多。 展开更多
关键词 鼻咽癌 放化疗敏感性 CUE结构域2蛋白 流式细胞术
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