Objective To detect HPV16 E6, E7 genes and cell-type specific enhancer （CTSE） of long control region （LCR） in breast carcinoma （BC）. Methods HPV16 E6 ,E7 genes and CTSE were detected in 40 BCs and 20 normal brea...Objective To detect HPV16 E6, E7 genes and cell-type specific enhancer （CTSE） of long control region （LCR） in breast carcinoma （BC）. Methods HPV16 E6 ,E7 genes and CTSE were detected in 40 BCs and 20 normal breast tissue （NBT） using polymerase chain reaction （PCR）. Results The positive rates of HPV16 E6 , E7genes and CTSE were 60% （ 24/40 ） , 55% （ 22/40 ） and 67. 5% （ 27/40 ） respectively in BCs, whereas only 5% （1/20）, 5% （1/20） and 15% （3/20） in NBTs （ P 〈 O. 05）. There exited significant correlation between E6 gene and CTSE in BCs （ P 〈0. 05） , as well as E7 gene and CTSE. The infection of HPV16 E6, E7 and CTSE had no statistic relationship with pathological features. Conclusion There were HPV16 E6 , E7 genes and CTSE together in BCs and CTSE may play an important role in pathogenesis of BC.展开更多
基金National Nature and Science Foundation, China (30500600)
文摘Objective To detect HPV16 E6, E7 genes and cell-type specific enhancer （CTSE） of long control region （LCR） in breast carcinoma （BC）. Methods HPV16 E6 ,E7 genes and CTSE were detected in 40 BCs and 20 normal breast tissue （NBT） using polymerase chain reaction （PCR）. Results The positive rates of HPV16 E6 , E7genes and CTSE were 60% （ 24/40 ） , 55% （ 22/40 ） and 67. 5% （ 27/40 ） respectively in BCs, whereas only 5% （1/20）, 5% （1/20） and 15% （3/20） in NBTs （ P 〈 O. 05）. There exited significant correlation between E6 gene and CTSE in BCs （ P 〈0. 05） , as well as E7 gene and CTSE. The infection of HPV16 E6, E7 and CTSE had no statistic relationship with pathological features. Conclusion There were HPV16 E6 , E7 genes and CTSE together in BCs and CTSE may play an important role in pathogenesis of BC.
