Background:Previously,a chronic social defeat stress(CSDS)model has been widely-adopted for assessing depressive-like behaviors in animals.However,there is still room for improvement in the CSDS model to safeguard stu...Background:Previously,a chronic social defeat stress(CSDS)model has been widely-adopted for assessing depressive-like behaviors in animals.However,there is still room for improvement in the CSDS model to safeguard study accuracy and the wel-fare of lab rodents.Our study team developed a novel,standardized apparatus to induce CSDS in rodents and assessed the model's practical adaptability.Methods:An innovative CSDS cage apparatus and water bottle was designed.To evaluate the effectiveness of the newly developed tools,a variety of animal models,including the tail suspension test(TST),sucrose preference test,forced swimming test(FST),novelty-suppressed feeding test,female urine sniffing test,and open field test(OFT),were adopted to assess depressive-like behaviors in mice.Fluoxetine treat-ment was also administered to observe the reversal effect,as part of the validation.Results:The CSDS cage apparatus resulted in the manifestation of depressive-like behaviors in the model mice.Significant reductions in sucrose preference and urine sniffing time were observed,while the OFT revealed decreased central zone total distance,residence time,and frequency of entry.Moreover,increased immobility was found in the FST and TST.Fluoxetine treatment was found to successfully reverse the modeling effect.Conclusion:The CSDS cage apparatus was validated for enhanced usability and ad-dressed the previous challenges of water bottle leakage and lab rodent welfare is-sues.The consistent results from multiple behavioral tests also supported real-world application of the apparatus,offering researchers a promising alternative to conven-tional rodent cages.展开更多
A high-precision CFD/CSD(Computational Fluid Dynamics/Computational Structural Dynamics)coupling method is developed to study the aeroelastic behavior and design the vibration reduction strategy of NTBT(New Type Blade...A high-precision CFD/CSD(Computational Fluid Dynamics/Computational Structural Dynamics)coupling method is developed to study the aeroelastic behavior and design the vibration reduction strategy of NTBT(New Type Blade-Tip)rotor with TEF(Trailing Edge Flap)technology in forward flight.In the aspect of CSD method,the closed-form dynamical governing equation is modified using Hamilton’s principle to consider the influence of the movable TEF,in which the NTBT geometric nonlinearity is considered through coordinate transformation by virtue of finite element method.In the aspect of CFD method,a moving-embedded grid method for rotor blades is developed to account for the dynamic deflection of TEF,in which the grid deformation is achieved through algebraic transformations,and a high-precision unsteady CFD method with 5th-order TENO(Targeted Essentially Non-Oscillatory)scheme is introduced to effectively simulate the rotor flowfield.Considering the computational efficiency,the loosely-coupling strategy is introduced to build up the CFD/CSD method.The validity of the coupling method is verified by comparing the computed aerodynamic loads,frequency spectrum,and structural loads with the referential or the experimental results of the typical model rotors.Based on that,the frequency,phase,and amplitude-sweeping parametric analyses of TEF are conducted thoroughly to reveal the influence mechanisms on the aeroelastic characteristics of NTBT rotor.Furthermore,an optimal control strategy is proposed to suppress the vibration intensity of hub loads,showing that the active vibration reduction method can effectively suppress the rotor hub vibratory intensity by over 40%in typical forward flight conditions.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:82204958 and 82305065Natural Science Foundation of Shandong Province,Grant/Award Number:ZR2020ZD17+2 种基金Traditional Chinese Medicine Emotional Disease and Brain Steady State Regulation Innovation Team,Grant/Award Number:2023KJ191Medical and Health Science and Technology Development Plan Project of Shandong Province,Grant/Award Number:202105010467Traditional Chinese Medicine Science and Technology Project of Shandong Province,Grant/Award Number:Q-2022059。
文摘Background:Previously,a chronic social defeat stress(CSDS)model has been widely-adopted for assessing depressive-like behaviors in animals.However,there is still room for improvement in the CSDS model to safeguard study accuracy and the wel-fare of lab rodents.Our study team developed a novel,standardized apparatus to induce CSDS in rodents and assessed the model's practical adaptability.Methods:An innovative CSDS cage apparatus and water bottle was designed.To evaluate the effectiveness of the newly developed tools,a variety of animal models,including the tail suspension test(TST),sucrose preference test,forced swimming test(FST),novelty-suppressed feeding test,female urine sniffing test,and open field test(OFT),were adopted to assess depressive-like behaviors in mice.Fluoxetine treat-ment was also administered to observe the reversal effect,as part of the validation.Results:The CSDS cage apparatus resulted in the manifestation of depressive-like behaviors in the model mice.Significant reductions in sucrose preference and urine sniffing time were observed,while the OFT revealed decreased central zone total distance,residence time,and frequency of entry.Moreover,increased immobility was found in the FST and TST.Fluoxetine treatment was found to successfully reverse the modeling effect.Conclusion:The CSDS cage apparatus was validated for enhanced usability and ad-dressed the previous challenges of water bottle leakage and lab rodent welfare is-sues.The consistent results from multiple behavioral tests also supported real-world application of the apparatus,offering researchers a promising alternative to conven-tional rodent cages.
基金supported by the National Natural Science Foundation of China(Nos.12102186,12472237)the Young Elite Scientists Sponsorship Program by CAST,China(No.2022QNRC001)+1 种基金the National Key Laboratory Foundation of China(No.61422202201)the Aeronautical Science Foundation of China(No.2024Z010052002)。
文摘A high-precision CFD/CSD(Computational Fluid Dynamics/Computational Structural Dynamics)coupling method is developed to study the aeroelastic behavior and design the vibration reduction strategy of NTBT(New Type Blade-Tip)rotor with TEF(Trailing Edge Flap)technology in forward flight.In the aspect of CSD method,the closed-form dynamical governing equation is modified using Hamilton’s principle to consider the influence of the movable TEF,in which the NTBT geometric nonlinearity is considered through coordinate transformation by virtue of finite element method.In the aspect of CFD method,a moving-embedded grid method for rotor blades is developed to account for the dynamic deflection of TEF,in which the grid deformation is achieved through algebraic transformations,and a high-precision unsteady CFD method with 5th-order TENO(Targeted Essentially Non-Oscillatory)scheme is introduced to effectively simulate the rotor flowfield.Considering the computational efficiency,the loosely-coupling strategy is introduced to build up the CFD/CSD method.The validity of the coupling method is verified by comparing the computed aerodynamic loads,frequency spectrum,and structural loads with the referential or the experimental results of the typical model rotors.Based on that,the frequency,phase,and amplitude-sweeping parametric analyses of TEF are conducted thoroughly to reveal the influence mechanisms on the aeroelastic characteristics of NTBT rotor.Furthermore,an optimal control strategy is proposed to suppress the vibration intensity of hub loads,showing that the active vibration reduction method can effectively suppress the rotor hub vibratory intensity by over 40%in typical forward flight conditions.
