电视行业如今已经逐步迈入了高清时代,各大高校所培养出的相关专业人才也要求具备高清电视节目的相关知识与技术,这就需要在教学中既要有理论方面的学习,也要有实践方面的锻炼机会。就如何创新性地利用高校现有资源(Adobe Premiere Pro ...电视行业如今已经逐步迈入了高清时代,各大高校所培养出的相关专业人才也要求具备高清电视节目的相关知识与技术,这就需要在教学中既要有理论方面的学习,也要有实践方面的锻炼机会。就如何创新性地利用高校现有资源(Adobe Premiere Pro CS4非编软件及较低硬件配置的计算机)完成高清电视节目的非编剪辑进行了探讨。展开更多
The members of the fourth subgroup of R2R3-MYB(Sg4 members)are well-known inhibitors of phenylpropanoid and lignin synthesis pathways.The C2 domain is closely related to the transcriptional inhibitory activity of Sg4 ...The members of the fourth subgroup of R2R3-MYB(Sg4 members)are well-known inhibitors of phenylpropanoid and lignin synthesis pathways.The C2 domain is closely related to the transcriptional inhibitory activity of Sg4 members.Phosphorylation modification enhances the transcriptional inhibitory activity of Sg4 members.Here,we identified a phosphorylation site on the C2 domain of Cs MYB4a from tea plants(Camellia sinensis).A mitogen-activated protein kinase(MAPK),named Cs MPK3-2,phosphorylated this site on the C2 domain of Cs MYB4a.Further experiments revealed that phosphorylation of Cs MYB4a weakened its ability to inhibit the gene expression of PAL,C4H,and 4CL in the phenylpropanoid pathway and activated the expression of transcription factor YABBY5,maintaining the adaxial-abaxial polarity of the leaf.Knocking out Nt YAB5 in Cs MYB4a transgenic tobacco partially repaired the leaf wrinkling phenotype caused by Cs MYB4a.The C1 domain exhibited an activation function when the C2 domain of Cs MYB4a was phosphorylated by Cs MPK3-2,causing this reversal phenomenon.These results enrich our understanding of the regulatory diversity of Sg4 members.展开更多
文摘电视行业如今已经逐步迈入了高清时代,各大高校所培养出的相关专业人才也要求具备高清电视节目的相关知识与技术,这就需要在教学中既要有理论方面的学习,也要有实践方面的锻炼机会。就如何创新性地利用高校现有资源(Adobe Premiere Pro CS4非编软件及较低硬件配置的计算机)完成高清电视节目的非编剪辑进行了探讨。
基金supported by the joint funds of National Natural Science Foundation of China(Grant Nos.U21A20232,32372756,32072621)。
文摘The members of the fourth subgroup of R2R3-MYB(Sg4 members)are well-known inhibitors of phenylpropanoid and lignin synthesis pathways.The C2 domain is closely related to the transcriptional inhibitory activity of Sg4 members.Phosphorylation modification enhances the transcriptional inhibitory activity of Sg4 members.Here,we identified a phosphorylation site on the C2 domain of Cs MYB4a from tea plants(Camellia sinensis).A mitogen-activated protein kinase(MAPK),named Cs MPK3-2,phosphorylated this site on the C2 domain of Cs MYB4a.Further experiments revealed that phosphorylation of Cs MYB4a weakened its ability to inhibit the gene expression of PAL,C4H,and 4CL in the phenylpropanoid pathway and activated the expression of transcription factor YABBY5,maintaining the adaxial-abaxial polarity of the leaf.Knocking out Nt YAB5 in Cs MYB4a transgenic tobacco partially repaired the leaf wrinkling phenotype caused by Cs MYB4a.The C1 domain exhibited an activation function when the C2 domain of Cs MYB4a was phosphorylated by Cs MPK3-2,causing this reversal phenomenon.These results enrich our understanding of the regulatory diversity of Sg4 members.
