本文旨在对3个影响猪达100 kg体重日龄(Age at 100 kg,AGE)和达100 kg体重平均日增重(Average Daily Gain at 100 kg,ADG)的候选基因进行生物信息学分析。运用生物信息学数据库及其软件,分别分析了肉碱O-乙酰转移酶(Carnitine O-acetylt...本文旨在对3个影响猪达100 kg体重日龄(Age at 100 kg,AGE)和达100 kg体重平均日增重(Average Daily Gain at 100 kg,ADG)的候选基因进行生物信息学分析。运用生物信息学数据库及其软件,分别分析了肉碱O-乙酰转移酶(Carnitine O-acetyltransferase,CRAT)、G蛋白偶联受体107(G Protein-Coupled Receptor 107,GPR107)和泛素特异性肽酶20(Ubiquitin Specific Peptidase 20,USP20)3个基因与其编码的产物。结果发现:CRAT基因位于猪的1号染色体,具有15个外显子、14个内含子,共编码626个氨基酸;USP20基因位于猪的1号染色体,具有26个外显子,25个内含子,共编码915个氨基酸;GPR107基因位于猪的1号染色体,具有19个外显子、18个内含子,共编码597个氨基酸;3个蛋白的二级结构主要组成均为无规则卷曲,且均表现出疏水特征,含有磷酸化位点,仅GPR107蛋白检测到信号肽和跨膜结构;猪CRAT、USP20、GPR107在进化过程中与其他哺乳动物之间均具有高度保守性以及序列同源性;CRAT与羟基类固醇17-β脱氢酶4(Hydroxysteroid 17-Beta Dehydrogenase 4,HSD17B4)、囊泡乙酰胆碱转运蛋白(Solute Carrier Family 18 Member A3,SLC18A3)、脂肪酶(Patatin Like Phospholipase Domain Containing 2,PNPLA2)等蛋白互作;USP20与STAM结合蛋白样1(STAM Binding Protein Like 1,STAMBPL1)、β2-肾上腺素能受体(Adrenoceptor Beta 2,ADRB2)、泛素特异性肽酶14(Ubiquitin Specific Peptidase 14,USP14)等蛋白互作;GPR107与LIN52(Lin-52 Homolog,LIN52)、P-选择素(Selectin P,SELP)、G蛋白偶联受体137C(G Protein-Coupled Receptor 137C,GPR137C)等蛋白互作;GO富集分析发现CRAT、USP20、GPR107主要参与脂肪酸的分解代谢、蛋白质稳定性和降解、配子生成过程;CRAT基因在肌肉组织中高表达,USP20基因在下丘脑中高表达,GPR107基因在血管和下丘脑中高表达。本文结果为进一步研究猪生长性状相关的功能基因提供了数据与理论参考。展开更多
The functional traits of soil fauna are closely related to ecosystem functions.The gut microbiota,which can reflect environmental changes,may be associated with functional traits.Therefore,in this study,collembolan(En...The functional traits of soil fauna are closely related to ecosystem functions.The gut microbiota,which can reflect environmental changes,may be associated with functional traits.Therefore,in this study,collembolan(Entomobrya proxima)was used to clarify the linkage response of specific gut taxa and traits under long-term urea exposure.A small amount of urea had positive effects on functional traits of E.proxima.Chao1 and Shannon indices of gut bacteria conditionally rare or abundant taxa(CRAT)gradually decreased under low and medium fertilizer,while increased under high fertilizer.Shannon index of abundant taxa(AT)showed a similar trend to that of CRAT except that the value of Shannon index was higher at high fertilizer than that of medium treatments.The structure and community assembly of CRAT changed significantly,and with the increase of urea addition amount,the dominant mechanism of community assembly changed from a deterministic process to a stochastic process.The niche width of AT and CRAT decreased.Relative abundance of some genera in AT and CRAT was closely related to functional traits.In conclusion,CRAT was more sensitive to urea than AT,had the potential to characterize functional traits of E.proxima,which will provide a basis for predicting the changes of soil animal traits and functions under the change of agricultural fertilizer strategy in the future.展开更多
文摘本文旨在对3个影响猪达100 kg体重日龄(Age at 100 kg,AGE)和达100 kg体重平均日增重(Average Daily Gain at 100 kg,ADG)的候选基因进行生物信息学分析。运用生物信息学数据库及其软件,分别分析了肉碱O-乙酰转移酶(Carnitine O-acetyltransferase,CRAT)、G蛋白偶联受体107(G Protein-Coupled Receptor 107,GPR107)和泛素特异性肽酶20(Ubiquitin Specific Peptidase 20,USP20)3个基因与其编码的产物。结果发现:CRAT基因位于猪的1号染色体,具有15个外显子、14个内含子,共编码626个氨基酸;USP20基因位于猪的1号染色体,具有26个外显子,25个内含子,共编码915个氨基酸;GPR107基因位于猪的1号染色体,具有19个外显子、18个内含子,共编码597个氨基酸;3个蛋白的二级结构主要组成均为无规则卷曲,且均表现出疏水特征,含有磷酸化位点,仅GPR107蛋白检测到信号肽和跨膜结构;猪CRAT、USP20、GPR107在进化过程中与其他哺乳动物之间均具有高度保守性以及序列同源性;CRAT与羟基类固醇17-β脱氢酶4(Hydroxysteroid 17-Beta Dehydrogenase 4,HSD17B4)、囊泡乙酰胆碱转运蛋白(Solute Carrier Family 18 Member A3,SLC18A3)、脂肪酶(Patatin Like Phospholipase Domain Containing 2,PNPLA2)等蛋白互作;USP20与STAM结合蛋白样1(STAM Binding Protein Like 1,STAMBPL1)、β2-肾上腺素能受体(Adrenoceptor Beta 2,ADRB2)、泛素特异性肽酶14(Ubiquitin Specific Peptidase 14,USP14)等蛋白互作;GPR107与LIN52(Lin-52 Homolog,LIN52)、P-选择素(Selectin P,SELP)、G蛋白偶联受体137C(G Protein-Coupled Receptor 137C,GPR137C)等蛋白互作;GO富集分析发现CRAT、USP20、GPR107主要参与脂肪酸的分解代谢、蛋白质稳定性和降解、配子生成过程;CRAT基因在肌肉组织中高表达,USP20基因在下丘脑中高表达,GPR107基因在血管和下丘脑中高表达。本文结果为进一步研究猪生长性状相关的功能基因提供了数据与理论参考。
基金funded by the Scientific and Technological Innovation Project of the Chinese Academy of Agricultural Sciences(Grant No.CAASZDRW202408).
文摘The functional traits of soil fauna are closely related to ecosystem functions.The gut microbiota,which can reflect environmental changes,may be associated with functional traits.Therefore,in this study,collembolan(Entomobrya proxima)was used to clarify the linkage response of specific gut taxa and traits under long-term urea exposure.A small amount of urea had positive effects on functional traits of E.proxima.Chao1 and Shannon indices of gut bacteria conditionally rare or abundant taxa(CRAT)gradually decreased under low and medium fertilizer,while increased under high fertilizer.Shannon index of abundant taxa(AT)showed a similar trend to that of CRAT except that the value of Shannon index was higher at high fertilizer than that of medium treatments.The structure and community assembly of CRAT changed significantly,and with the increase of urea addition amount,the dominant mechanism of community assembly changed from a deterministic process to a stochastic process.The niche width of AT and CRAT decreased.Relative abundance of some genera in AT and CRAT was closely related to functional traits.In conclusion,CRAT was more sensitive to urea than AT,had the potential to characterize functional traits of E.proxima,which will provide a basis for predicting the changes of soil animal traits and functions under the change of agricultural fertilizer strategy in the future.
