Ischemic stroke is increasing worldwide,and stent intervention has gradually become one of the effective treatments.Owing to its good mechanical properties and biocompatibility,biodegradable Mg-Zn-Y-Nd alloy(ZE21B)has...Ischemic stroke is increasing worldwide,and stent intervention has gradually become one of the effective treatments.Owing to its good mechanical properties and biocompatibility,biodegradable Mg-Zn-Y-Nd alloy(ZE21B)has good application prospects in vascular stents.However,too fast degradation and delayed endothelialization are the bottleneck problem to limit its further application.In this study,the bio-inspired coating barnacle cement cp19k is constructed on ZE21B surface through electrostatic spraying to improve the corrosion resistance and pro-endothelialization ability.The coating was evaluated through electrochemistry,static immersion corrosion,in vitro blood experiments and cell experiments.Cp19k effectively improved the corrosion resistance of the substrate ZE21B,reduced the degradation rate,improved endothelial cell proliferation and migration capabilities,inhibited smooth muscle cells proliferation and regulated contractile phenotype,inhibited macrophage adhesion,regulated macrophage M2 phenotype,and reduced the expression of inflammatory factor of TNF-a and inhibited fibroplasia in vivo.Our data indicated that the barnacle cement cp19k coating had potential application on the surface modification of Mg alloy cerebrovascular stent.展开更多
SONY VAIO，一个充满活力、个性张扬的品牌，每隔一年便会推出全新的系列，且每次都让人有焕然一新的感觉。日前，SONY再次对VAIO产品线进行了重新定位和调整，推出了包括A、X、S和TR四个系列共十款机型，其中的A系列便是VAIO的新旗舰...SONY VAIO，一个充满活力、个性张扬的品牌，每隔一年便会推出全新的系列，且每次都让人有焕然一新的感觉。日前，SONY再次对VAIO产品线进行了重新定位和调整，推出了包括A、X、S和TR四个系列共十款机型，其中的A系列便是VAIO的新旗舰级产品。经过与SONY的积极联系，我们在第一时间试用和测试了SONY VAIO VGN—A11C国内行货版。展开更多
Ischemic stroke is on the rise worldwide,and stent intervention is gradually becoming one of the effective treatments.Biodegradable Mg-Zn-Y-Nd alloy(ZE21B)has good mechanical properties and biocompatibility,and has a ...Ischemic stroke is on the rise worldwide,and stent intervention is gradually becoming one of the effective treatments.Biodegradable Mg-Zn-Y-Nd alloy(ZE21B)has good mechanical properties and biocompatibility,and has a good application prospect in vascular scaffolds.However,it still suffers from poor corrosion resistance,insufficient endothelialization,and blood-brain barrier(BBB)remodeling.Previously,it was found that constructing a bionic coating of barnacle gum protein cp19k on the surface of ZE21B by electrostatic spraying could improve its corrosion resistance and pro-endothelialization ability.Therefore,we considered piggybacking sulfonated hyaluronic acid nanoparticles(NP@S-HA)on the coating,which inherited the excellent anticoagulant and anti-inflammatory properties of S-HA on the basis of its improved corrosion resistance.The coatings were evaluated by electrochemistry,static immersion corrosion,in vitro blood experiments,and cellular experiments.Collectively,the cp19k/NP@S-HA coating demonstrated an average corrosion resistance enhancement of approximately 40.6%relative to the uncoated ZE21B.Cp19k/NP@S-HA was able to effectively improve the proliferation and migration of endothelial cells,inhibit the proliferation and regulate the contractile phenotype of smooth muscle cells,inhibit macrophage(MA)adherence,regulate the M2 phenotype of MAs,reduce the expression of the inflammatory factor tumor necrosis factor-α(TNF-α),and inhibit fibroplasia.In summary,the composite coatings developed in this study provide an effective strategy for surface modification of magnesium alloys in cerebrovascular applications,which has a broad application prospect.展开更多
基金financially supported by the National Key Research and Development Program of China(No.2021YFC2400703)the Joint Fund of the National Natural Science Foundation of China(No.U2004164)+1 种基金Key Scientific and Technological Research Projects in Henan Province(Nos.232102311155 and 232102230106)Zhengzhou University Major Project Cultivation Special Project(No.125-32214076)。
文摘Ischemic stroke is increasing worldwide,and stent intervention has gradually become one of the effective treatments.Owing to its good mechanical properties and biocompatibility,biodegradable Mg-Zn-Y-Nd alloy(ZE21B)has good application prospects in vascular stents.However,too fast degradation and delayed endothelialization are the bottleneck problem to limit its further application.In this study,the bio-inspired coating barnacle cement cp19k is constructed on ZE21B surface through electrostatic spraying to improve the corrosion resistance and pro-endothelialization ability.The coating was evaluated through electrochemistry,static immersion corrosion,in vitro blood experiments and cell experiments.Cp19k effectively improved the corrosion resistance of the substrate ZE21B,reduced the degradation rate,improved endothelial cell proliferation and migration capabilities,inhibited smooth muscle cells proliferation and regulated contractile phenotype,inhibited macrophage adhesion,regulated macrophage M2 phenotype,and reduced the expression of inflammatory factor of TNF-a and inhibited fibroplasia in vivo.Our data indicated that the barnacle cement cp19k coating had potential application on the surface modification of Mg alloy cerebrovascular stent.
基金supported by the National Key Research and Development Program of China(2021YFC2400703)Zhengzhou University Major Project Cultivation Special Project(125-32214076)。
文摘Ischemic stroke is on the rise worldwide,and stent intervention is gradually becoming one of the effective treatments.Biodegradable Mg-Zn-Y-Nd alloy(ZE21B)has good mechanical properties and biocompatibility,and has a good application prospect in vascular scaffolds.However,it still suffers from poor corrosion resistance,insufficient endothelialization,and blood-brain barrier(BBB)remodeling.Previously,it was found that constructing a bionic coating of barnacle gum protein cp19k on the surface of ZE21B by electrostatic spraying could improve its corrosion resistance and pro-endothelialization ability.Therefore,we considered piggybacking sulfonated hyaluronic acid nanoparticles(NP@S-HA)on the coating,which inherited the excellent anticoagulant and anti-inflammatory properties of S-HA on the basis of its improved corrosion resistance.The coatings were evaluated by electrochemistry,static immersion corrosion,in vitro blood experiments,and cellular experiments.Collectively,the cp19k/NP@S-HA coating demonstrated an average corrosion resistance enhancement of approximately 40.6%relative to the uncoated ZE21B.Cp19k/NP@S-HA was able to effectively improve the proliferation and migration of endothelial cells,inhibit the proliferation and regulate the contractile phenotype of smooth muscle cells,inhibit macrophage(MA)adherence,regulate the M2 phenotype of MAs,reduce the expression of the inflammatory factor tumor necrosis factor-α(TNF-α),and inhibit fibroplasia.In summary,the composite coatings developed in this study provide an effective strategy for surface modification of magnesium alloys in cerebrovascular applications,which has a broad application prospect.
