As a traditional Chinese herbal medicine component,Corydalis yanhusuo is gradually attracting the attention of the skin care industry.In this study,Corydalis yanhusuo root extract dispersion(CTR50)was used as the rese...As a traditional Chinese herbal medicine component,Corydalis yanhusuo is gradually attracting the attention of the skin care industry.In this study,Corydalis yanhusuo root extract dispersion(CTR50)was used as the research object.The related activities of Corydalis yanhusuo root extract dispersion were studied by cytotoxicity test,in vitro and in vivo antioxidant test and cell scratch healing test.The results showed that the dispersion of Corydalis yanhusuo root extract had no cytotoxicity to HaCaT cells.When the concentration of Corydalis yanhusuo root extract dispersion was 5%,the scavenging rate of DPPH free radical was 35.05%,and the scavenging ability of hydroxyl radical was weak;CTR50 can significantly increase the activity of SOD in HaCaT cells,increase the content of antioxidant factor GSH in HaCaT cells induced by UVB,and improve the oxidative stability of skin;it significantly promoted the healing ability of HaCaT cells at the scratch site.It can be seen that the dispersion of Corydalis yanhusuo root extract has the potential to be used as a functional raw material for natural skin care products,which provides a theoretical basis for its development and application in the field of cosmetics.展开更多
A new protoberberine alkaloid, named 5,6-dihydro- 10-hydroxy-2,3,9-trimethoxy- 13-methyldibenzo[α,g]quinoliziniurn （1） was isolated from the 60% ethanol extract of the tubers of Corydalis yanhusuo W. T. Wang, toget...A new protoberberine alkaloid, named 5,6-dihydro- 10-hydroxy-2,3,9-trimethoxy- 13-methyldibenzo[α,g]quinoliziniurn （1） was isolated from the 60% ethanol extract of the tubers of Corydalis yanhusuo W. T. Wang, together with a new natural product, 13methylpalmatrubine （2）. Their structures were established by spectroscopic methods. 2009 Xin Sheng Yao. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved.展开更多
A new nitro tetrahydronprotoberberins alkaloid, 1-nitro-apocavidine was isolated from Corydalis saxicola Bunting. The structure was established by spectroscopic methods.
(+)/(-)-Yanhusuosines A(1) and B(2), two pairs of trace benzylisoquinoline-protoberberine atropoenantiomeric homodimers featuring an unprecedented 6/7/6/6/6/6 hexacyclic skeleton, were isolated from the tubers of Cory...(+)/(-)-Yanhusuosines A(1) and B(2), two pairs of trace benzylisoquinoline-protoberberine atropoenantiomeric homodimers featuring an unprecedented 6/7/6/6/6/6 hexacyclic skeleton, were isolated from the tubers of Corydalis yanhusuo. The structures of(+)/(-)-1 and(+)/(-)-2 were elucidated using spectroscopic and quantum-chemical calculation approaches.(+)/(-)-Yanhusuosines A(1) and B(2)represent a new class of alkaloid dimers biogenetically constructed by a molecule of benzylisoquinoline with a unit of protoberberine via an intermolecular [4 + 3] cycloaddition. Their plausible biosynthetic pathways are discussed, and compound 2 exerted moderate inhibitory activity of NO formation in LPS induced RAW264.7 macrophages.展开更多
Deciphering the metabolites of multiple components in herbal medicine has far-reaching significance for revealing pharmacodynamic ingredients.However,most chemical components of herbal medicine are secondary metabolit...Deciphering the metabolites of multiple components in herbal medicine has far-reaching significance for revealing pharmacodynamic ingredients.However,most chemical components of herbal medicine are secondary metabolites with low content whose in vivo metabolites are close to trace amounts,making it difficult to achieve comprehensive detection and identification.In this paper,an efficient strategy was proposed:herb-derived metabolites were predicted according to the structural characteristics and metabolic reactions of chemical constituents in Corydalis Rhizoma and chemical structure screening tables for metabolites were conducted.The fragmentation patterns were summarized from representative standards combining with specific cleavage behaviors to deduce structures of metabolites.Ion abundance plays an important role in compound identification,and high ion abundance can improve identification accuracy.The types of metabolites in different biological samples were very similar,but their ion abundance might be different.Therefore,for trace metabolites in biological samples,we used the following two methods to process:metabolites of high dose herbal extract were analyzed to characterize those of clinical dose herbal extracts in the same biological samples;cross-mapping of different biological samples was applied to identify trace metabolites based on the fact that a metabolite has different ion abundance in different biological samples.Compared with not using this strategy,44 more metabolites of clinical dose herbal extract were detected.This study improved the depth,breadth,and accuracy of current methods for herb-derived metabolites characterization.展开更多
Phytochemical investigation of the vinegar-prepared Corydalis yanhusuo led to the isolation of one aristolactam derivative,1,2,8,9-tetramethoxy-5-methyldibenzo[cd,f]indol-4-(5H)-one(1),and seven aporphine alkaloids,in...Phytochemical investigation of the vinegar-prepared Corydalis yanhusuo led to the isolation of one aristolactam derivative,1,2,8,9-tetramethoxy-5-methyldibenzo[cd,f]indol-4-(5H)-one(1),and seven aporphine alkaloids,including 2,9,10-trimethoxydibenz[de,g]quinolin-7-one(2),1-hydroxy-2,9,10-trimethoxy-7H-dibenzo(de,g)quinoline-7-one(3),oxoglaucine(4),N-methyloxoglaucine trifluoroacetate trifluoroacetate(5),corunine acetate(6),pontevedrine(7),and oxoglaucidaline trifluoroacetate(8).The structures of the isolated compounds were elucidated by extensive spectroscopic data analysis and comparison with the previous reports.Among them,compounds 1 and 2 were obtained as natural products for the first time,and their NMR data were unambiguously assigned.In addition,compound 1 exhibited moderate cytotoxic activity against HepG2 cells with an IC50 value of 16.0±6.4μM.展开更多
Chief ComponentsRhizoma Corydalis,Radix AngelicaeDahuricae.Rationale of the PrescriptionRhizoma Corydalis acts on both theblood and the qi systems to invigorate bloodcirculation,regulate the flow of qi and alle-viate ...Chief ComponentsRhizoma Corydalis,Radix AngelicaeDahuricae.Rationale of the PrescriptionRhizoma Corydalis acts on both theblood and the qi systems to invigorate bloodcirculation,regulate the flow of qi and alle-viate pain.It is thus effective in treating paindue to blood stasis and qi stagnation.展开更多
[Objectives] To establish the quality standard of Corydalis tomentella Franch. [Methods] The medicinal materials are identified from the perspectives of morphology,characters,microscopic characteristics and TLC of the...[Objectives] To establish the quality standard of Corydalis tomentella Franch. [Methods] The medicinal materials are identified from the perspectives of morphology,characters,microscopic characteristics and TLC of the plants. The contents of moisture,total ash and water extract are determined. The content of dehydrocavidine is determined by HPLC. The determination is performed on Agilent C18 column with mobile phase consisted of acetonitrile-phosphate buffer solution( containing 20 mmol/L monopotassium phosphate,10 mmol/L diethylamine,0. 1% phosphoric acid)( 28∶ 72,V/V) at the flow rate of 1. 0 m L/min. The detection wavelength is set at 347 nm,and the column temperature is 35℃. The sample size is 10 μL. [Results] The original plants are perennial herbs,and as produced into medicinal materials,they are hairy and shrink into balls with conical principal roots,and obvious cracks on the tubers and roots,curved and fragmented leaves,yellowish white flowers. The quasi-circular pollen grains contain square and columnar crystals,there are substantial nonglandular hairs,and also bordered pits,spiral and reticulate vessels,as well as wood fibers. TLC spots are clear and well-separated. The content of moisture is7. 5%-18. 5%,total ash 20. 5%-26. 2%,and water extract 29. 9%-46. 4%. The linear range of dehydrocavidine is 0. 040 08-2. 404 8 μg( r =0. 999 9); RSD of precision,stability and reproducibility tests is lower than 2. 0%. The recoveries are 95. 6%-102. 5%( RSD =2. 3%,n = 9). [Conclusions] The established standard can be used for quality evaluation of C. tomentella Franch..展开更多
[Objectives]To establish the quality standard of Tibetan medicine Corydalis dasyptera Maxim.[Methods]According to the research method of drug quality standard in the appendix of 2020 edition of Chinese Pharmacopoeia,8...[Objectives]To establish the quality standard of Tibetan medicine Corydalis dasyptera Maxim.[Methods]According to the research method of drug quality standard in the appendix of 2020 edition of Chinese Pharmacopoeia,8 batches of C.dasyptera Maxim.from different habitats were studied by character identification,microscopic identification and TLC identification.The content of water,total ash,acid-insoluble ash and alcohol-soluble extract was determined,and the content of protopine in medicinal materials was determined by high performance liquid chromatography(HPLC).[Results]The properties and microscopic characteristics of C.dasyptera Maxim.were determined.The TLC characteristic spots of the medicinal materials were clear,the degree of separation was good,and the specificity was strong.Both the test sample and the control sample showed the same yellow-green spots in the corresponding position.It was tentatively determined that the water content of C.dasyptera Maxim.should not exceed 14.0%,the total ash content should not exceed 14.0%,the acid-insoluble ash content should not exceed 3.0%,and the alcohol-soluble extract content should not be less than 18.0%.There was a good linear relationship between the concentration of protopine and the peak area in the range of 16.64-166.40μg·10-3(r=0.9996).The average recovery rate was 98.47%and the RSD was 1.21%(n=6).The content of protopine in 8 batches of C.dasyptera Maxim.was 0.023%-0.093%.[Conclusions]The established quality research method is simple,stable and reliable,and can be used for the quality control of C.dasyptera Maxim.展开更多
Corydalis bungeana Turcz.(CB)is a medicinal herb with significant medicinal value in traditional Chinese medicine.This paper reviews the progress of research on CB’s botany,quality control,phytochemistry,pharmacology...Corydalis bungeana Turcz.(CB)is a medicinal herb with significant medicinal value in traditional Chinese medicine.This paper reviews the progress of research on CB’s botany,quality control,phytochemistry,pharmacology and toxicity.The plant’s information was gathered from scientific databases such as PubMed,GeenMedical,Springer Link(https://link.springer.com),Chinese National Knowledge Infrastructure,Pharmacopoeia and Flora.Currently,137 phytochemicals have been identified and isolated from CB,including alkaloids,flavonoids,amino acids,terpenoids,coumarins and organic acids.In addition,many phytochemicals reported various antiinflammatory,antibacterial,antiviral,antitumor,analgesic,hepatoprotective,immunomodulatory,neuromodulatory,and lipid reduction activities.However,the study of its toxicity is still at the preliminary exploration stage and needs further intensive exploration.Herein,we provide an in-depth investigation of the progress of CB to elucidate the underlying mechanisms of activity of CB extracts and its major components,deliver valuable resources and information for further research and rational drug use,and explore the potential research directions and prospects of CB.展开更多
In order to better control common diseases of corn and soybean in Sanjiang plain,the in vitro antifungal activity of corydalis chloroform extract against several common fungal diseases of corn and soybeans was tested ...In order to better control common diseases of corn and soybean in Sanjiang plain,the in vitro antifungal activity of corydalis chloroform extract against several common fungal diseases of corn and soybeans was tested using mycelial growth rate method. The test result of mycelial growth rate showed that the inhibition rate of corydalis chloroform extract against Cercosporidium sofinum（ Hara） Liu ＆ Guo and Rhizoctonia solani Kuhn were all above 80%,and EC50 values were52. 98- 58. 98 mg /L. The test result of spore germination showed that the inhibition rates against spore germination of all pathogenic fungi were high,in which the inhibition rates against spore germination of C. sofinum and Bipolaris maydis reached 100%. Biological control test showed that the control effects against other diseases were below 60% except that against R. solani reaching 61. 46%.展开更多
The molecular mechanism underlying Corydalis Yanhusuo’s therapeutic potential in prostate cancer(PCa)treatment was elucidated using network pharmacology and molecular docking.Nineteen active ingredients,399 drug targ...The molecular mechanism underlying Corydalis Yanhusuo’s therapeutic potential in prostate cancer(PCa)treatment was elucidated using network pharmacology and molecular docking.Nineteen active ingredients,399 drug targets,1790 disease targets and 143 intersection targets were identified.Ten core targets were screened from the protein-protein interaction network.Enrichment analysis revealed 133 GO terms and 114 KEGG pathways.Corydalis Yanhusuo may potentially treat prostate cancer through pathways such as the Rap1 signaling pathway,phospholipase D signaling pathway,Ras signaling pathway,VEGF signaling pathway and JAK-STAT signaling pathway.Significant differences in expression were observed for EGFR,PDGFRA,PIK3CA,PIK3CD,PIK3CG and PIK3R1.Molecular docking and dynamics simulation analysis showed low binding energy between active components and the six core genes of Corydalis Yanhusuo,indicating a favorable docking effect.This study shows that Corydalis Yanhusuo exhibits promise in prostate cancer treatment through a synergistic“multi-component-multi-target-multi-pathway”effect.展开更多
[Objectives]To investigate the protective effects of Siwu Xuanhu decoction on the liver of mice with acute liver injury induced by CCl 4.[Methods]A total of 48 ICR mice were randomly assigned to six groups:a blank con...[Objectives]To investigate the protective effects of Siwu Xuanhu decoction on the liver of mice with acute liver injury induced by CCl 4.[Methods]A total of 48 ICR mice were randomly assigned to six groups:a blank control group,a model group,a low dose group of Siwu Xuanhu decoction(100 mg/kg),a medium dose group of Siwu Xuanhu decoction(200 mg/kg),a high dose group of Siwu Xuanhu decoction(300 mg/kg),and a positive control group(silymarin 2 mg/kg).Each group comprised 8 mice.Each dosing group received the designated dose of the drug(10 mL/kg)via continuous gavage,while the blank group and the model group were administered an equivalent volume of normal saline for four weeks,three times per week.An acute liver injury model was established through the intraperitoneal administration of a 20%CCl 4 olive oil solution at a dosage of 2 mL/kg in all experimental groups,with the exception of the control group,which received an equivalent volume of the olive oil solution.After 24 h fasting with water intake,blood samples were collected from the ocular region.The blood samples were allowed to stand and subsequently subjected to centrifugation to isolate the upper layer of serum,and a diagnostic kit was employed to measure the levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT)and interleukin-6(IL-6),and the activity of superoxide dismutase(SOD)in the serum.[Results]In comparison to the control group,serum levels of AST and ALT were significantly elevated in the model group.Furthermore,when compared to the model group,treatment with Siwu Xuanhu decoction resulted in a reduction of serum levels of ALT,AST,and IL-6,while simultaneously increasing SOD activities.[Conclusions]Siwu Xuanhu decoction exhibits a protective effect against acute liver injury induced by CCl 4 in mice.展开更多
Since alkaloids are the major active constituents of Rhizoma corydalis(RC),a convenient and accurate analytical method is needed for their identification and characterization.Here we report a method to profile the alk...Since alkaloids are the major active constituents of Rhizoma corydalis(RC),a convenient and accurate analytical method is needed for their identification and characterization.Here we report a method to profile the alkaloids in RC based on liquid chromatography-tandem quadrupole time-of-flight mass spectrometry(LC–Q-TOF-MS/MS).A total of 16 alkaloids belonging to four different classes were identified by comparison with authentic standards.The fragmentation pathway of each class of alkaloid was clarified and their differences were elucidated.Furthermore,based on an analysis of fragmentation pathways and alkaloid profiling,a rapid and accurate method for the identification of unknown alkaloids in RC is proposed.The method could also be useful for the quality control of RC.展开更多
In hermaphroditic plants, female reproductive success often varies among different positions within an inflorescence. However, few studies have evaluated the relative importance of underlying causes such as pollen lim...In hermaphroditic plants, female reproductive success often varies among different positions within an inflorescence. However, few studies have evaluated the relative importance of underlying causes such as pollen limitation, resource limitation or architectural effect, and few have compared male allocation. During a 2-year investigation, we found that female reproductive success of an acropetally flowering species, Corydalis remota Fisch. ex Maxim. var. lineariloba Maxim. was significantly lower in the upper late developing flowers when compared with the lower early flowers. Supplementation with outcross pollen did not improve female reproductive success of the upper flowers, while removal of the lower developing fruits significantly increased female reproductive success of the upper flowers in both years, evidencing resource limitation of the upper flowers. Female production in upper flowers was greatly improved by simultaneous pollen supplementation of the upper flowers and removal of the lower fruits, suggesting that, when resources are abundant, pollen may limit the female reproductive success of the upper flowers. The less seed mass in the upper flowers didn＇t increase in all treatments due to architecture. In the upper flowers, ovule production was significantly lower and the pollen ： ovule ratio was significantly higher. These results suggest that male-biased sex allocation in the upper flowers may lead to increased male reproductive success, whereas the lower flowers have higher female reproductive success.展开更多
The genus Corydalis, with ca. 530 species, has long been considered taxonomically challenging because of its great variability. Previous molecular analyses,based on a few molecular markers and incomplete taxonomic sam...The genus Corydalis, with ca. 530 species, has long been considered taxonomically challenging because of its great variability. Previous molecular analyses,based on a few molecular markers and incomplete taxonomic sampling, were clearly inadequate to delimit sections and subgenera. We have performed phylogenetic analyses of Corydalis and related taxa,using 65 shared protein-coding plastid genes from313 accessions(including 280 samples of ca. 226species of Corydalis) and 152 universal low-copy nuclear genes from 296 accessions(including 271samples of Corydalis) covering all 42 previously recognized sections and five independent “series”.Phylogenetic trees were inferred using Bayesian Inference and Maximum Likelihood. Eight selected morphological characters were estimated using ancestral state reconstructions. Results include:(i) of the three subgenera of Corydalis, two are fully supported by both the plastid and nuclear data;the third,subg. Cremnocapnos, is weakly supported by plastid DNA only, whereas in the nuclear data the two included sections form successive outgroups to the rest of the genus;(ii) among all 42 sections and five“series”, 25 sections and one “series” are resolved as monophyletic in both data sets;(iii) the common ancestor of Corydalis is likely to be a perennial plant with a taproot, yellow flowers with a short saccate spur, linear fruits with recurved fruiting pedicels, and seeds with elaiosomes;(iv) we provide a new classification of Corydalis with four subgenera(of which subg. Bipapillatae is here newly described), 39 sections, 16 of which are consistent with the previous classification, 16 sections have been recircumscribed, one section has been reinstated and six new sections are established. Characters associated with lifespan, underground structures, floral spur, fruit and elaiosomes are important for the recognition of subgenera and sections. These new phylogenetic analyses combined with ancestral character reconstructions uncovered previously unrecognized relationships, and greatly improved our understanding of the evolution of the genus.展开更多
Objective To clarify the rationality and to provide reasonable usage references of processed Corydalis Rhizoma （CR） in clinic by comparing respectively pharmacological actions including analgesic, anti-inflammation,...Objective To clarify the rationality and to provide reasonable usage references of processed Corydalis Rhizoma （CR） in clinic by comparing respectively pharmacological actions including analgesic, anti-inflammation, antiplatelet aggregation and spasmolysis of Yuanhu Zhitong Tablet （YZT） and Yanhusuo Decoction （YD） constituted with raw CR （RCR） or processed CR （PCR）. Methods The hot-board, acetic-acid- induced twisting experiments and mouse auricular swelling model by injecting xylene in the abdomen of mouse were adopted to investigate the analgesic and anti-inflammatory effects of the four formulas on Kunming mice; The experiment of contractile activity on isolated rat intestine smooth muscle on Wistar rats was applied to observing the spasmolysis effect of the four formulas; The experiments of platelet aggregation induced by ADP or collagen on Wistar rats by turbidimetry method were used to observe the anti-platelet aggregation effect of the four formulas. Results Compared with RCR in two prescriptions, PCR displayed better analgesic effect （P 〈 0.05, 0.01）, while there was no difference on anti-inflammatory effect; Inhibition of the two prescriptions on smooth muscle spasm was presented well, and the prescription YD with PCR was remarkable （P 〈 0.05, 0.01）; Compared with the blank group, the two prescriptions showed outstanding effects on anti-platelet aggregation （P〈 0.01）, but no difference between RCR and PCR in two prescriptions. Conclusion PCR can improve the analgesic effect of prescription YZT and intensify the spasmolysis effect of prescription YD, which can be explained by the Chinese medicine processing regulation. Therefore, PCR is suggested to be chosen in YZT and YD used to cure pain symptoms.展开更多
Objective:Corydalis bungeana(CB)is a well-used medicinal herb in Mongolian folk medicine and has been traditionally applied as an antiobesity agent.However,the evidence-based pharmacological effects of CB and its spec...Objective:Corydalis bungeana(CB)is a well-used medicinal herb in Mongolian folk medicine and has been traditionally applied as an antiobesity agent.However,the evidence-based pharmacological effects of CB and its specific metabolic alterations in the obese model are not entirely understood.This study aimed to utilize untargeted metabolomic techniques to identify biomarkers and gain mechanistic insight into the serum metabolite alterations associated with weight loss and lipid metabolism in obese rats.Methods:A high-fat high-sugar(HFHS)diet was used to induce obese models in rats.CB extract was orally gavaged at 0.18,0.9 and 1.8 g/kg doses for six weeks,and feed intake,body weight,fat pad weight,and blood indexes were measured.Blood serum metabolites were evaluated by gas chromatography/quadrupole time-of-fight tandem mass spectrometry(GC-TOF/MS).Results:The results showed that compared with the obese group,the administration of CB extract caused significant decreases in body weight(P<0.05),feed intake,Lee’s index,and perirenal,mesenteric,epididymal fat weight.CB extract also reduced blood triglyceride and total cholesterol levels(P<0.05)of obese rats.Metabolomic findings showed that nine differential metabolites,including pyruvic acid,D-glucuronic acid,malic acid,dimethylglycine,oxoglutaric acid,pantothenic acid,sorbitol acid,fumaric acid and glucose 6-phosphate were identified under CB treatment and altered metabolic pathways such as TCA cycle,pantothenate and CoA biosynthesis,and glycolysis/gluconeogenesis.Conclusion:This study demonstrated weight loss and lipid lowering effects of CB on HFHS diet-induced obese rats and identified nine metabolites as potential biomarkers for evaluating the favorable therapeutic mechanism of CB via regulation of lipid and glucose metabolism.展开更多
文摘As a traditional Chinese herbal medicine component,Corydalis yanhusuo is gradually attracting the attention of the skin care industry.In this study,Corydalis yanhusuo root extract dispersion(CTR50)was used as the research object.The related activities of Corydalis yanhusuo root extract dispersion were studied by cytotoxicity test,in vitro and in vivo antioxidant test and cell scratch healing test.The results showed that the dispersion of Corydalis yanhusuo root extract had no cytotoxicity to HaCaT cells.When the concentration of Corydalis yanhusuo root extract dispersion was 5%,the scavenging rate of DPPH free radical was 35.05%,and the scavenging ability of hydroxyl radical was weak;CTR50 can significantly increase the activity of SOD in HaCaT cells,increase the content of antioxidant factor GSH in HaCaT cells induced by UVB,and improve the oxidative stability of skin;it significantly promoted the healing ability of HaCaT cells at the scratch site.It can be seen that the dispersion of Corydalis yanhusuo root extract has the potential to be used as a functional raw material for natural skin care products,which provides a theoretical basis for its development and application in the field of cosmetics.
文摘A new protoberberine alkaloid, named 5,6-dihydro- 10-hydroxy-2,3,9-trimethoxy- 13-methyldibenzo[α,g]quinoliziniurn （1） was isolated from the 60% ethanol extract of the tubers of Corydalis yanhusuo W. T. Wang, together with a new natural product, 13methylpalmatrubine （2）. Their structures were established by spectroscopic methods. 2009 Xin Sheng Yao. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved.
基金This project was supported by the 863 Foundation of China(NO.2003AA2Z3507)Scientific Foundation ofShanghai China(NO.02DZ19147,01DJ19010).
文摘A new nitro tetrahydronprotoberberins alkaloid, 1-nitro-apocavidine was isolated from Corydalis saxicola Bunting. The structure was established by spectroscopic methods.
基金supported by the National Natural Science Foundation of China (No. 82073978)the Fundamental Research Funds for the Central Universities (No. 2022-JYB-JBZR-015)Beijing Natural Science Foundation (No. JQ18026)。
文摘(+)/(-)-Yanhusuosines A(1) and B(2), two pairs of trace benzylisoquinoline-protoberberine atropoenantiomeric homodimers featuring an unprecedented 6/7/6/6/6/6 hexacyclic skeleton, were isolated from the tubers of Corydalis yanhusuo. The structures of(+)/(-)-1 and(+)/(-)-2 were elucidated using spectroscopic and quantum-chemical calculation approaches.(+)/(-)-Yanhusuosines A(1) and B(2)represent a new class of alkaloid dimers biogenetically constructed by a molecule of benzylisoquinoline with a unit of protoberberine via an intermolecular [4 + 3] cycloaddition. Their plausible biosynthetic pathways are discussed, and compound 2 exerted moderate inhibitory activity of NO formation in LPS induced RAW264.7 macrophages.
文摘Deciphering the metabolites of multiple components in herbal medicine has far-reaching significance for revealing pharmacodynamic ingredients.However,most chemical components of herbal medicine are secondary metabolites with low content whose in vivo metabolites are close to trace amounts,making it difficult to achieve comprehensive detection and identification.In this paper,an efficient strategy was proposed:herb-derived metabolites were predicted according to the structural characteristics and metabolic reactions of chemical constituents in Corydalis Rhizoma and chemical structure screening tables for metabolites were conducted.The fragmentation patterns were summarized from representative standards combining with specific cleavage behaviors to deduce structures of metabolites.Ion abundance plays an important role in compound identification,and high ion abundance can improve identification accuracy.The types of metabolites in different biological samples were very similar,but their ion abundance might be different.Therefore,for trace metabolites in biological samples,we used the following two methods to process:metabolites of high dose herbal extract were analyzed to characterize those of clinical dose herbal extracts in the same biological samples;cross-mapping of different biological samples was applied to identify trace metabolites based on the fact that a metabolite has different ion abundance in different biological samples.Compared with not using this strategy,44 more metabolites of clinical dose herbal extract were detected.This study improved the depth,breadth,and accuracy of current methods for herb-derived metabolites characterization.
基金This work was financially supported by Beijing Natural Science Foundation(Grant No.JQ18026)the National Key R&D Program of China(Grant No.2017YFC1700400)+1 种基金the National Natural Science Foundation of China(Grant No.82073978)the Fundamental Research Funds for the Central Universities(Grant No.2021-BUCMXJKY007).
文摘Phytochemical investigation of the vinegar-prepared Corydalis yanhusuo led to the isolation of one aristolactam derivative,1,2,8,9-tetramethoxy-5-methyldibenzo[cd,f]indol-4-(5H)-one(1),and seven aporphine alkaloids,including 2,9,10-trimethoxydibenz[de,g]quinolin-7-one(2),1-hydroxy-2,9,10-trimethoxy-7H-dibenzo(de,g)quinoline-7-one(3),oxoglaucine(4),N-methyloxoglaucine trifluoroacetate trifluoroacetate(5),corunine acetate(6),pontevedrine(7),and oxoglaucidaline trifluoroacetate(8).The structures of the isolated compounds were elucidated by extensive spectroscopic data analysis and comparison with the previous reports.Among them,compounds 1 and 2 were obtained as natural products for the first time,and their NMR data were unambiguously assigned.In addition,compound 1 exhibited moderate cytotoxic activity against HepG2 cells with an IC50 value of 16.0±6.4μM.
文摘Chief ComponentsRhizoma Corydalis,Radix AngelicaeDahuricae.Rationale of the PrescriptionRhizoma Corydalis acts on both theblood and the qi systems to invigorate bloodcirculation,regulate the flow of qi and alle-viate pain.It is thus effective in treating paindue to blood stasis and qi stagnation.
基金Supported by National Key Science and Technology Project(2014ZX09304307-2)
文摘[Objectives] To establish the quality standard of Corydalis tomentella Franch. [Methods] The medicinal materials are identified from the perspectives of morphology,characters,microscopic characteristics and TLC of the plants. The contents of moisture,total ash and water extract are determined. The content of dehydrocavidine is determined by HPLC. The determination is performed on Agilent C18 column with mobile phase consisted of acetonitrile-phosphate buffer solution( containing 20 mmol/L monopotassium phosphate,10 mmol/L diethylamine,0. 1% phosphoric acid)( 28∶ 72,V/V) at the flow rate of 1. 0 m L/min. The detection wavelength is set at 347 nm,and the column temperature is 35℃. The sample size is 10 μL. [Results] The original plants are perennial herbs,and as produced into medicinal materials,they are hairy and shrink into balls with conical principal roots,and obvious cracks on the tubers and roots,curved and fragmented leaves,yellowish white flowers. The quasi-circular pollen grains contain square and columnar crystals,there are substantial nonglandular hairs,and also bordered pits,spiral and reticulate vessels,as well as wood fibers. TLC spots are clear and well-separated. The content of moisture is7. 5%-18. 5%,total ash 20. 5%-26. 2%,and water extract 29. 9%-46. 4%. The linear range of dehydrocavidine is 0. 040 08-2. 404 8 μg( r =0. 999 9); RSD of precision,stability and reproducibility tests is lower than 2. 0%. The recoveries are 95. 6%-102. 5%( RSD =2. 3%,n = 9). [Conclusions] The established standard can be used for quality evaluation of C. tomentella Franch..
基金Chunhui Project—Identification of the Source of Tibetan Medicine C.dasyptera Maxim.
文摘[Objectives]To establish the quality standard of Tibetan medicine Corydalis dasyptera Maxim.[Methods]According to the research method of drug quality standard in the appendix of 2020 edition of Chinese Pharmacopoeia,8 batches of C.dasyptera Maxim.from different habitats were studied by character identification,microscopic identification and TLC identification.The content of water,total ash,acid-insoluble ash and alcohol-soluble extract was determined,and the content of protopine in medicinal materials was determined by high performance liquid chromatography(HPLC).[Results]The properties and microscopic characteristics of C.dasyptera Maxim.were determined.The TLC characteristic spots of the medicinal materials were clear,the degree of separation was good,and the specificity was strong.Both the test sample and the control sample showed the same yellow-green spots in the corresponding position.It was tentatively determined that the water content of C.dasyptera Maxim.should not exceed 14.0%,the total ash content should not exceed 14.0%,the acid-insoluble ash content should not exceed 3.0%,and the alcohol-soluble extract content should not be less than 18.0%.There was a good linear relationship between the concentration of protopine and the peak area in the range of 16.64-166.40μg·10-3(r=0.9996).The average recovery rate was 98.47%and the RSD was 1.21%(n=6).The content of protopine in 8 batches of C.dasyptera Maxim.was 0.023%-0.093%.[Conclusions]The established quality research method is simple,stable and reliable,and can be used for the quality control of C.dasyptera Maxim.
基金This research was funded by the National Natural Science Foundation of China(82260773)National Key R&D Program:Intergovernmental Cooperation in International Science and Technology Innovation(2022YFE0119300)+1 种基金Central Government Guided Local Scientific and Technological Development Project(2021ZY0015)Science and Technology Young Talents Development Project of Inner Mongolia Autonomous Region(NJYT22048).
文摘Corydalis bungeana Turcz.(CB)is a medicinal herb with significant medicinal value in traditional Chinese medicine.This paper reviews the progress of research on CB’s botany,quality control,phytochemistry,pharmacology and toxicity.The plant’s information was gathered from scientific databases such as PubMed,GeenMedical,Springer Link(https://link.springer.com),Chinese National Knowledge Infrastructure,Pharmacopoeia and Flora.Currently,137 phytochemicals have been identified and isolated from CB,including alkaloids,flavonoids,amino acids,terpenoids,coumarins and organic acids.In addition,many phytochemicals reported various antiinflammatory,antibacterial,antiviral,antitumor,analgesic,hepatoprotective,immunomodulatory,neuromodulatory,and lipid reduction activities.However,the study of its toxicity is still at the preliminary exploration stage and needs further intensive exploration.Herein,we provide an in-depth investigation of the progress of CB to elucidate the underlying mechanisms of activity of CB extracts and its major components,deliver valuable resources and information for further research and rational drug use,and explore the potential research directions and prospects of CB.
基金Supported by Agricultural Science and Technology Innovation Project of Hei-longjiang Province"Inhibitory Effect of Corydalis Chloroform Extract againstSeveral Pathogenic Fungi"(2012QN007)
文摘In order to better control common diseases of corn and soybean in Sanjiang plain,the in vitro antifungal activity of corydalis chloroform extract against several common fungal diseases of corn and soybeans was tested using mycelial growth rate method. The test result of mycelial growth rate showed that the inhibition rate of corydalis chloroform extract against Cercosporidium sofinum（ Hara） Liu ＆ Guo and Rhizoctonia solani Kuhn were all above 80%,and EC50 values were52. 98- 58. 98 mg /L. The test result of spore germination showed that the inhibition rates against spore germination of all pathogenic fungi were high,in which the inhibition rates against spore germination of C. sofinum and Bipolaris maydis reached 100%. Biological control test showed that the control effects against other diseases were below 60% except that against R. solani reaching 61. 46%.
基金supported by local special projects in major health of Hubei Provincial Science and Technology Department(2022BCE054)key scientific research projects of Hubei Polytechnic University(23xjz08A)Hubei Polytechnic University·Huangshi Daye Lake high-tech Zone University Science Park Joint Open Fund Project(23xjz04AK).
文摘The molecular mechanism underlying Corydalis Yanhusuo’s therapeutic potential in prostate cancer(PCa)treatment was elucidated using network pharmacology and molecular docking.Nineteen active ingredients,399 drug targets,1790 disease targets and 143 intersection targets were identified.Ten core targets were screened from the protein-protein interaction network.Enrichment analysis revealed 133 GO terms and 114 KEGG pathways.Corydalis Yanhusuo may potentially treat prostate cancer through pathways such as the Rap1 signaling pathway,phospholipase D signaling pathway,Ras signaling pathway,VEGF signaling pathway and JAK-STAT signaling pathway.Significant differences in expression were observed for EGFR,PDGFRA,PIK3CA,PIK3CD,PIK3CG and PIK3R1.Molecular docking and dynamics simulation analysis showed low binding energy between active components and the six core genes of Corydalis Yanhusuo,indicating a favorable docking effect.This study shows that Corydalis Yanhusuo exhibits promise in prostate cancer treatment through a synergistic“multi-component-multi-target-multi-pathway”effect.
基金Supported by Undergraduate Innovation and Entrepreneurship Training Program of Guangxi Zhuang Autonomous Region(S202310599089)Open Fund Project of Key Laboratory of Guangxi Universities on National Medicine in Youjiang River Basin(yykf2024-04).
文摘[Objectives]To investigate the protective effects of Siwu Xuanhu decoction on the liver of mice with acute liver injury induced by CCl 4.[Methods]A total of 48 ICR mice were randomly assigned to six groups:a blank control group,a model group,a low dose group of Siwu Xuanhu decoction(100 mg/kg),a medium dose group of Siwu Xuanhu decoction(200 mg/kg),a high dose group of Siwu Xuanhu decoction(300 mg/kg),and a positive control group(silymarin 2 mg/kg).Each group comprised 8 mice.Each dosing group received the designated dose of the drug(10 mL/kg)via continuous gavage,while the blank group and the model group were administered an equivalent volume of normal saline for four weeks,three times per week.An acute liver injury model was established through the intraperitoneal administration of a 20%CCl 4 olive oil solution at a dosage of 2 mL/kg in all experimental groups,with the exception of the control group,which received an equivalent volume of the olive oil solution.After 24 h fasting with water intake,blood samples were collected from the ocular region.The blood samples were allowed to stand and subsequently subjected to centrifugation to isolate the upper layer of serum,and a diagnostic kit was employed to measure the levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT)and interleukin-6(IL-6),and the activity of superoxide dismutase(SOD)in the serum.[Results]In comparison to the control group,serum levels of AST and ALT were significantly elevated in the model group.Furthermore,when compared to the model group,treatment with Siwu Xuanhu decoction resulted in a reduction of serum levels of ALT,AST,and IL-6,while simultaneously increasing SOD activities.[Conclusions]Siwu Xuanhu decoction exhibits a protective effect against acute liver injury induced by CCl 4 in mice.
基金The work was funded by the National Natural Science Foundation of China(Nos.81202950 and 81102795)National Key Project of the Scientific and Technical Supporting Programs of China(No.2012ZX10004301-608).
文摘Since alkaloids are the major active constituents of Rhizoma corydalis(RC),a convenient and accurate analytical method is needed for their identification and characterization.Here we report a method to profile the alkaloids in RC based on liquid chromatography-tandem quadrupole time-of-flight mass spectrometry(LC–Q-TOF-MS/MS).A total of 16 alkaloids belonging to four different classes were identified by comparison with authentic standards.The fragmentation pathway of each class of alkaloid was clarified and their differences were elucidated.Furthermore,based on an analysis of fragmentation pathways and alkaloid profiling,a rapid and accurate method for the identification of unknown alkaloids in RC is proposed.The method could also be useful for the quality control of RC.
基金Supported by the National Natural Science Foundation of China (30430160).
文摘In hermaphroditic plants, female reproductive success often varies among different positions within an inflorescence. However, few studies have evaluated the relative importance of underlying causes such as pollen limitation, resource limitation or architectural effect, and few have compared male allocation. During a 2-year investigation, we found that female reproductive success of an acropetally flowering species, Corydalis remota Fisch. ex Maxim. var. lineariloba Maxim. was significantly lower in the upper late developing flowers when compared with the lower early flowers. Supplementation with outcross pollen did not improve female reproductive success of the upper flowers, while removal of the lower developing fruits significantly increased female reproductive success of the upper flowers in both years, evidencing resource limitation of the upper flowers. Female production in upper flowers was greatly improved by simultaneous pollen supplementation of the upper flowers and removal of the lower fruits, suggesting that, when resources are abundant, pollen may limit the female reproductive success of the upper flowers. The less seed mass in the upper flowers didn＇t increase in all treatments due to architecture. In the upper flowers, ovule production was significantly lower and the pollen ： ovule ratio was significantly higher. These results suggest that male-biased sex allocation in the upper flowers may lead to increased male reproductive success, whereas the lower flowers have higher female reproductive success.
基金supported by grants from the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (2019QZKK0502)the Key R&D Program of Yunnan Province (202103AF140005)+6 种基金the National Natural Science Foundation of China (32170215 and 32100187)the National Natural Science Foundation of China-Yunnan Joint Fund to Support Key Projects (U1802232)Strategic Priority Research Program of Chinese Academy of Sciences(XDA20050203)International Partnership Program of Chinese Academy of Sciences (151853KYSB20180009)Yunnan Young&Elite Talents Project (YNWR-QNBJ-2019-033)Ten Thousand Talents Program of Yunnan Province (202005AB160005)Chinese Academy of Sciences “Light of West China” Program。
文摘The genus Corydalis, with ca. 530 species, has long been considered taxonomically challenging because of its great variability. Previous molecular analyses,based on a few molecular markers and incomplete taxonomic sampling, were clearly inadequate to delimit sections and subgenera. We have performed phylogenetic analyses of Corydalis and related taxa,using 65 shared protein-coding plastid genes from313 accessions(including 280 samples of ca. 226species of Corydalis) and 152 universal low-copy nuclear genes from 296 accessions(including 271samples of Corydalis) covering all 42 previously recognized sections and five independent “series”.Phylogenetic trees were inferred using Bayesian Inference and Maximum Likelihood. Eight selected morphological characters were estimated using ancestral state reconstructions. Results include:(i) of the three subgenera of Corydalis, two are fully supported by both the plastid and nuclear data;the third,subg. Cremnocapnos, is weakly supported by plastid DNA only, whereas in the nuclear data the two included sections form successive outgroups to the rest of the genus;(ii) among all 42 sections and five“series”, 25 sections and one “series” are resolved as monophyletic in both data sets;(iii) the common ancestor of Corydalis is likely to be a perennial plant with a taproot, yellow flowers with a short saccate spur, linear fruits with recurved fruiting pedicels, and seeds with elaiosomes;(iv) we provide a new classification of Corydalis with four subgenera(of which subg. Bipapillatae is here newly described), 39 sections, 16 of which are consistent with the previous classification, 16 sections have been recircumscribed, one section has been reinstated and six new sections are established. Characters associated with lifespan, underground structures, floral spur, fruit and elaiosomes are important for the recognition of subgenera and sections. These new phylogenetic analyses combined with ancestral character reconstructions uncovered previously unrecognized relationships, and greatly improved our understanding of the evolution of the genus.
基金National Basic Research Program of China(81073053)The State Administration of Traditional Chinese Medicine Scientific Research(20110700709)
文摘Objective To clarify the rationality and to provide reasonable usage references of processed Corydalis Rhizoma （CR） in clinic by comparing respectively pharmacological actions including analgesic, anti-inflammation, antiplatelet aggregation and spasmolysis of Yuanhu Zhitong Tablet （YZT） and Yanhusuo Decoction （YD） constituted with raw CR （RCR） or processed CR （PCR）. Methods The hot-board, acetic-acid- induced twisting experiments and mouse auricular swelling model by injecting xylene in the abdomen of mouse were adopted to investigate the analgesic and anti-inflammatory effects of the four formulas on Kunming mice; The experiment of contractile activity on isolated rat intestine smooth muscle on Wistar rats was applied to observing the spasmolysis effect of the four formulas; The experiments of platelet aggregation induced by ADP or collagen on Wistar rats by turbidimetry method were used to observe the anti-platelet aggregation effect of the four formulas. Results Compared with RCR in two prescriptions, PCR displayed better analgesic effect （P 〈 0.05, 0.01）, while there was no difference on anti-inflammatory effect; Inhibition of the two prescriptions on smooth muscle spasm was presented well, and the prescription YD with PCR was remarkable （P 〈 0.05, 0.01）; Compared with the blank group, the two prescriptions showed outstanding effects on anti-platelet aggregation （P〈 0.01）, but no difference between RCR and PCR in two prescriptions. Conclusion PCR can improve the analgesic effect of prescription YZT and intensify the spasmolysis effect of prescription YD, which can be explained by the Chinese medicine processing regulation. Therefore, PCR is suggested to be chosen in YZT and YD used to cure pain symptoms.
基金supported by China National Natural Science Foundation Project (No.81803845)Inner Mongolia Autonomous Region Natural Science Foundation Project (No.2018MS08040)+2 种基金"Mongolian Medicine Food and Drug Source Protection and Utilization Innovation Team" Construction Project (No.190301)Mongolian Medicine Standardization Research International Cooperation Scienceand Technology Innovation project(No.MDKBZH2018009)Open Project of Key Laboratory of Mongolian Medicine Research and Development project of Ministry of Education (No.MDK2018056)
文摘Objective:Corydalis bungeana(CB)is a well-used medicinal herb in Mongolian folk medicine and has been traditionally applied as an antiobesity agent.However,the evidence-based pharmacological effects of CB and its specific metabolic alterations in the obese model are not entirely understood.This study aimed to utilize untargeted metabolomic techniques to identify biomarkers and gain mechanistic insight into the serum metabolite alterations associated with weight loss and lipid metabolism in obese rats.Methods:A high-fat high-sugar(HFHS)diet was used to induce obese models in rats.CB extract was orally gavaged at 0.18,0.9 and 1.8 g/kg doses for six weeks,and feed intake,body weight,fat pad weight,and blood indexes were measured.Blood serum metabolites were evaluated by gas chromatography/quadrupole time-of-fight tandem mass spectrometry(GC-TOF/MS).Results:The results showed that compared with the obese group,the administration of CB extract caused significant decreases in body weight(P<0.05),feed intake,Lee’s index,and perirenal,mesenteric,epididymal fat weight.CB extract also reduced blood triglyceride and total cholesterol levels(P<0.05)of obese rats.Metabolomic findings showed that nine differential metabolites,including pyruvic acid,D-glucuronic acid,malic acid,dimethylglycine,oxoglutaric acid,pantothenic acid,sorbitol acid,fumaric acid and glucose 6-phosphate were identified under CB treatment and altered metabolic pathways such as TCA cycle,pantothenate and CoA biosynthesis,and glycolysis/gluconeogenesis.Conclusion:This study demonstrated weight loss and lipid lowering effects of CB on HFHS diet-induced obese rats and identified nine metabolites as potential biomarkers for evaluating the favorable therapeutic mechanism of CB via regulation of lipid and glucose metabolism.
