[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DN...[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=0.999.Nos terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.展开更多
Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace ...Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace of l1. contains a complemented subspace of l1 which is asymptotically isometric to l1. Let X be a separable Banach space such that X^＊ contains asymptotically isometric copies of lp （1 〈 p 〈∞）. Then there exists a quotient space of X which is asymptotically isometric to lq （1/p ＋ 1/q=1）. Complemented asymptotically isometric copies of co in K（X, Y） and W（X, Y） are discussed. Let X be a Gelfand-Phillips space. If X contains asymptotically isometric copies of co, it has to contain complemented asymptotically isometric copies of co.展开更多
Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest ...Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest and buying potentials resulted in a number of forgeries manufactured and introduced to the open antique market.The items such as early silver candlesticks dictate a very high price,for many high quality fakes show very good appearances and matching similarities with originals.Such copies are traditionally manufactured by casting using the original items as patterns.Small details and variances in design features,position and shape of hallmarks,including the final surface quality are usual features to distinguish the fakes from the originals.This paper presents results of a study conducted on several silver candlesticks,including two artefacts bearing features of those produced in the mid 18th century,one original Italian candelabrum from Fascist era,and small candlesticks made in the early 20th century.Also,the paper presents some interesting contemporary coins-replicas of many those produced in different countries.The coins were offered for sale by unscrupulous dealers via auctions and e-bays.Finally the main results and findings from this study are discussed from a manufacturing point of view,such as fabrication technology,surface quality and hallmarks,which will help the collectors,dealers and investors to detect and avoid forgeries.展开更多
目的通过对236例流产物进行低深度全基因组拷贝数变异检测(copy number variation sequencing,CNV-seq)及短串联重复序列(short tandem repeats,STR)连锁分析检测,探讨其在流产物染色体异常检测中的临床应用价值。方法采用CNV-seq和STR...目的通过对236例流产物进行低深度全基因组拷贝数变异检测(copy number variation sequencing,CNV-seq)及短串联重复序列(short tandem repeats,STR)连锁分析检测,探讨其在流产物染色体异常检测中的临床应用价值。方法采用CNV-seq和STR连锁分析对2021年3月-2024年8月于郑州大学第二附属医院产前诊断中心就诊的236例自然流产患者的流产物样本进行检测,确定具有临床意义的染色体异常并对其进行分析。结果6例流产物因严重母源污染退出研究,剩余230例流产物染色体异常的阳性率为62.61%(144/230),其中染色体非整倍体110例(69.44%),多倍体7例(4.86%),拷贝数变异27例(18.75%)。染色体非整倍体中以常染色体三体最为常见,其中16-三体最常见,其次是21-三体。高龄产妇染色体异常率显著高于低龄产妇,早期流产组染色体异常发生率显著高于晚期流产组,均以染色体非整倍体异常为主。结论染色体非整倍体是导致自然流产最常见的原因,孕妇的妊娠年龄与胎儿常染色体三体的发生密切相关。CNV-seq联合STR连锁分析对于流产物的染色体分析高效可靠,可作为流产物遗传学分析的常规方法。展开更多
基金Funded by Program of Technology Bureau of Harbin(2010RFQXN101)Sub-project of Transgenic Significant Specific Project(2008ZX08012-001)~~
文摘[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=0.999.Nos terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.
基金Supported by NSFC(10271060)NSFC(10171014) the Doctoral Programme Foundation of Institution of Higher Education(20010055013).
文摘Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace of l1. contains a complemented subspace of l1 which is asymptotically isometric to l1. Let X be a separable Banach space such that X^＊ contains asymptotically isometric copies of lp （1 〈 p 〈∞）. Then there exists a quotient space of X which is asymptotically isometric to lq （1/p ＋ 1/q=1）. Complemented asymptotically isometric copies of co in K（X, Y） and W（X, Y） are discussed. Let X be a Gelfand-Phillips space. If X contains asymptotically isometric copies of co, it has to contain complemented asymptotically isometric copies of co.
文摘Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest and buying potentials resulted in a number of forgeries manufactured and introduced to the open antique market.The items such as early silver candlesticks dictate a very high price,for many high quality fakes show very good appearances and matching similarities with originals.Such copies are traditionally manufactured by casting using the original items as patterns.Small details and variances in design features,position and shape of hallmarks,including the final surface quality are usual features to distinguish the fakes from the originals.This paper presents results of a study conducted on several silver candlesticks,including two artefacts bearing features of those produced in the mid 18th century,one original Italian candelabrum from Fascist era,and small candlesticks made in the early 20th century.Also,the paper presents some interesting contemporary coins-replicas of many those produced in different countries.The coins were offered for sale by unscrupulous dealers via auctions and e-bays.Finally the main results and findings from this study are discussed from a manufacturing point of view,such as fabrication technology,surface quality and hallmarks,which will help the collectors,dealers and investors to detect and avoid forgeries.
文摘目的通过对236例流产物进行低深度全基因组拷贝数变异检测(copy number variation sequencing,CNV-seq)及短串联重复序列(short tandem repeats,STR)连锁分析检测,探讨其在流产物染色体异常检测中的临床应用价值。方法采用CNV-seq和STR连锁分析对2021年3月-2024年8月于郑州大学第二附属医院产前诊断中心就诊的236例自然流产患者的流产物样本进行检测,确定具有临床意义的染色体异常并对其进行分析。结果6例流产物因严重母源污染退出研究,剩余230例流产物染色体异常的阳性率为62.61%(144/230),其中染色体非整倍体110例(69.44%),多倍体7例(4.86%),拷贝数变异27例(18.75%)。染色体非整倍体中以常染色体三体最为常见,其中16-三体最常见,其次是21-三体。高龄产妇染色体异常率显著高于低龄产妇,早期流产组染色体异常发生率显著高于晚期流产组,均以染色体非整倍体异常为主。结论染色体非整倍体是导致自然流产最常见的原因,孕妇的妊娠年龄与胎儿常染色体三体的发生密切相关。CNV-seq联合STR连锁分析对于流产物的染色体分析高效可靠,可作为流产物遗传学分析的常规方法。
