[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DN...[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=0.999.Nos terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.展开更多
Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace ...Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace of l1. contains a complemented subspace of l1 which is asymptotically isometric to l1. Let X be a separable Banach space such that X^＊ contains asymptotically isometric copies of lp （1 〈 p 〈∞）. Then there exists a quotient space of X which is asymptotically isometric to lq （1/p ＋ 1/q=1）. Complemented asymptotically isometric copies of co in K（X, Y） and W（X, Y） are discussed. Let X be a Gelfand-Phillips space. If X contains asymptotically isometric copies of co, it has to contain complemented asymptotically isometric copies of co.展开更多
Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest ...Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest and buying potentials resulted in a number of forgeries manufactured and introduced to the open antique market.The items such as early silver candlesticks dictate a very high price,for many high quality fakes show very good appearances and matching similarities with originals.Such copies are traditionally manufactured by casting using the original items as patterns.Small details and variances in design features,position and shape of hallmarks,including the final surface quality are usual features to distinguish the fakes from the originals.This paper presents results of a study conducted on several silver candlesticks,including two artefacts bearing features of those produced in the mid 18th century,one original Italian candelabrum from Fascist era,and small candlesticks made in the early 20th century.Also,the paper presents some interesting contemporary coins-replicas of many those produced in different countries.The coins were offered for sale by unscrupulous dealers via auctions and e-bays.Finally the main results and findings from this study are discussed from a manufacturing point of view,such as fabrication technology,surface quality and hallmarks,which will help the collectors,dealers and investors to detect and avoid forgeries.展开更多
Let n≥2 be a natural number,1≤p≤∞and X a Banach space.We prove that if X^(*)containsλ-uniformly copies of l^(k)^(p),then:P(^(n)X) contains cKλ^(n)-uniformly copies of■.in the case p^(*)>n(ii)P(^(n)X) contain...Let n≥2 be a natural number,1≤p≤∞and X a Banach space.We prove that if X^(*)containsλ-uniformly copies of l^(k)^(p),then:P(^(n)X) contains cKλ^(n)-uniformly copies of■.in the case p^(*)>n(ii)P(^(n)X) containsλ^(n)-uniformly copies of l^(k)_(∞)in the case p^(*)≤n.This complete a result of S.Dineen’s from 1995.展开更多
Via an insufficient coat protein complex I(COPI)retrieval signal,the majority of SARSCo V-2 spike(S)is resident in host early secretory organelles and a tiny amount is leaked out in cell surface.Only surface-exposed S...Via an insufficient coat protein complex I(COPI)retrieval signal,the majority of SARSCo V-2 spike(S)is resident in host early secretory organelles and a tiny amount is leaked out in cell surface.Only surface-exposed S can be recognized by B cell receptor(BCR)or anti-S therapeutic monoclonal antibodies(m Abs)that is the trigger step for B cell activation after S m RNA vaccination or infected cell clearance by S m Abs.Now,a drug strategy to promote S host surface exposure is absent.Here,we first combined structural and biochemical analysis to characterize S COPI sorting signals.A potent S COPI sorting inhibitor was then invented,evidently capable of promoting S surface exposure and facilitating infected cell clearance by S antibody-dependent cellular cytotoxicity(ADCC).Importantly,with the inhibitor as a probe,we revealed Omicron BA.1 S is less cell surface exposed than prototypes because of a constellation of S folding mutations,possibly corresponding to its ER chaperone association.Our findings not only suggest COPI is a druggable target against COVID-19,but also highlight SARS-Co V-2 evolution mechanism driven by S folding and trafficking mutations.展开更多
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of upper and lower motor neurons in the brainstem and spinal cord,leading to muscle weakness,para...Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of upper and lower motor neurons in the brainstem and spinal cord,leading to muscle weakness,paralysis,and respiratory failure (Morgan and Orrell,2016).展开更多
基金Funded by Program of Technology Bureau of Harbin(2010RFQXN101)Sub-project of Transgenic Significant Specific Project(2008ZX08012-001)~~
文摘[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=0.999.Nos terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.
基金Supported by NSFC(10271060)NSFC(10171014) the Doctoral Programme Foundation of Institution of Higher Education(20010055013).
文摘Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace of l1. contains a complemented subspace of l1 which is asymptotically isometric to l1. Let X be a separable Banach space such that X^＊ contains asymptotically isometric copies of lp （1 〈 p 〈∞）. Then there exists a quotient space of X which is asymptotically isometric to lq （1/p ＋ 1/q=1）. Complemented asymptotically isometric copies of co in K（X, Y） and W（X, Y） are discussed. Let X be a Gelfand-Phillips space. If X contains asymptotically isometric copies of co, it has to contain complemented asymptotically isometric copies of co.
文摘Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest and buying potentials resulted in a number of forgeries manufactured and introduced to the open antique market.The items such as early silver candlesticks dictate a very high price,for many high quality fakes show very good appearances and matching similarities with originals.Such copies are traditionally manufactured by casting using the original items as patterns.Small details and variances in design features,position and shape of hallmarks,including the final surface quality are usual features to distinguish the fakes from the originals.This paper presents results of a study conducted on several silver candlesticks,including two artefacts bearing features of those produced in the mid 18th century,one original Italian candelabrum from Fascist era,and small candlesticks made in the early 20th century.Also,the paper presents some interesting contemporary coins-replicas of many those produced in different countries.The coins were offered for sale by unscrupulous dealers via auctions and e-bays.Finally the main results and findings from this study are discussed from a manufacturing point of view,such as fabrication technology,surface quality and hallmarks,which will help the collectors,dealers and investors to detect and avoid forgeries.
文摘Let n≥2 be a natural number,1≤p≤∞and X a Banach space.We prove that if X^(*)containsλ-uniformly copies of l^(k)^(p),then:P(^(n)X) contains cKλ^(n)-uniformly copies of■.in the case p^(*)>n(ii)P(^(n)X) containsλ^(n)-uniformly copies of l^(k)_(∞)in the case p^(*)≤n.This complete a result of S.Dineen’s from 1995.
基金supported by Startup fund program at Beijing University of Chinese Medicine(BUCM)(90011451310011,China)to Wenfu Mathe emergency fund against COVID-19 program at BUCM(1000061223476,China)to Wenfu Mathe innovation team and talents cultivation program of national administration of traditional Chinese medicine(ZYYCXTD-C202006,China)to Wenfu Ma。
文摘Via an insufficient coat protein complex I(COPI)retrieval signal,the majority of SARSCo V-2 spike(S)is resident in host early secretory organelles and a tiny amount is leaked out in cell surface.Only surface-exposed S can be recognized by B cell receptor(BCR)or anti-S therapeutic monoclonal antibodies(m Abs)that is the trigger step for B cell activation after S m RNA vaccination or infected cell clearance by S m Abs.Now,a drug strategy to promote S host surface exposure is absent.Here,we first combined structural and biochemical analysis to characterize S COPI sorting signals.A potent S COPI sorting inhibitor was then invented,evidently capable of promoting S surface exposure and facilitating infected cell clearance by S antibody-dependent cellular cytotoxicity(ADCC).Importantly,with the inhibitor as a probe,we revealed Omicron BA.1 S is less cell surface exposed than prototypes because of a constellation of S folding mutations,possibly corresponding to its ER chaperone association.Our findings not only suggest COPI is a druggable target against COVID-19,but also highlight SARS-Co V-2 evolution mechanism driven by S folding and trafficking mutations.
文摘Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of upper and lower motor neurons in the brainstem and spinal cord,leading to muscle weakness,paralysis,and respiratory failure (Morgan and Orrell,2016).
