Chromatin modifications,including histone acetylation,play essential roles in regulating flowering.The CBP/p300 family HISTONE ACETYLTRANSFERASE 1(HAC1),which mediates histone acetylation,promotes the process of flora...Chromatin modifications,including histone acetylation,play essential roles in regulating flowering.The CBP/p300 family HISTONE ACETYLTRANSFERASE 1(HAC1),which mediates histone acetylation,promotes the process of floral transition;however,the precise mechanism remains largely unclear.Specifically,how HAC1 is involved in the flowering regulatory network and which genes are the direct targets of HAC1 during flowering regulation are still unknown.In this study,we elucidate the critical function of HAC1 in promoting flowering via exerting active epigenetic markers at two key floral integrators,FLOWERING LOCUS T(FT)and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1(SOC1),thereby regulating their expression to trigger the flowering process.We show that HAC1 physically interacts with CONSTANS(CO)in vivo and in vitro.Chromatin immunoprecipitation results indicate that HAC1 directly binds to the FT and SOC1 loci.Loss of HAC1 impairs CO-mediated transcriptional activation of FT and SOC1 in promoting flowering.Moreover,CO mutation leads to the decreased enrichment of HAC1 at FT and SOC1,indicating that CO recruits HAC1 to FT and SOC1.Finally,HAC1,as well as CO,is required for the elevated histone acetylation level at FT and SOC1.Taken together,our finding reveals that HAC1-mediated histone acetylation boots flowering via a CO-dependent activation of FT and SOC1.展开更多
为研究CONSTANS-LIKE 7(COL7)在调控植物开花方面的功能,以定量PCR,GUS染色等方法,研究光及生物钟对COL7表达的影响,以及COL7对拟南芥开花的影响.实验结果显示:光及生物钟参与调控COL7的表达;过表达COL7在长日照条件下抑制CONSTANS(CO)...为研究CONSTANS-LIKE 7(COL7)在调控植物开花方面的功能,以定量PCR,GUS染色等方法,研究光及生物钟对COL7表达的影响,以及COL7对拟南芥开花的影响.实验结果显示:光及生物钟参与调控COL7的表达;过表达COL7在长日照条件下抑制CONSTANS(CO)以及FLOWERING LOCUS T(FT)的表达,进而抑制拟南芥开花;col7突变体不论是在长日照下还是短日照下都没有明显的开花表型,说明COL7在调控拟南芥开化方面可能与其家族基因中的其它成员存在功能冗余.展开更多
基金supported by the National Natural Science Foundation of China to Z.L.(32300474)and to C.L.(32470346 and 32270362)the Guangdong Basic and Applied Basic Research Foundation to C.L.(2024A1515010612)the Fundamental Research Funds for the Central Universities to X.S.(24qnpy078).
文摘Chromatin modifications,including histone acetylation,play essential roles in regulating flowering.The CBP/p300 family HISTONE ACETYLTRANSFERASE 1(HAC1),which mediates histone acetylation,promotes the process of floral transition;however,the precise mechanism remains largely unclear.Specifically,how HAC1 is involved in the flowering regulatory network and which genes are the direct targets of HAC1 during flowering regulation are still unknown.In this study,we elucidate the critical function of HAC1 in promoting flowering via exerting active epigenetic markers at two key floral integrators,FLOWERING LOCUS T(FT)and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1(SOC1),thereby regulating their expression to trigger the flowering process.We show that HAC1 physically interacts with CONSTANS(CO)in vivo and in vitro.Chromatin immunoprecipitation results indicate that HAC1 directly binds to the FT and SOC1 loci.Loss of HAC1 impairs CO-mediated transcriptional activation of FT and SOC1 in promoting flowering.Moreover,CO mutation leads to the decreased enrichment of HAC1 at FT and SOC1,indicating that CO recruits HAC1 to FT and SOC1.Finally,HAC1,as well as CO,is required for the elevated histone acetylation level at FT and SOC1.Taken together,our finding reveals that HAC1-mediated histone acetylation boots flowering via a CO-dependent activation of FT and SOC1.
文摘为研究CONSTANS-LIKE 7(COL7)在调控植物开花方面的功能,以定量PCR,GUS染色等方法,研究光及生物钟对COL7表达的影响,以及COL7对拟南芥开花的影响.实验结果显示:光及生物钟参与调控COL7的表达;过表达COL7在长日照条件下抑制CONSTANS(CO)以及FLOWERING LOCUS T(FT)的表达,进而抑制拟南芥开花;col7突变体不论是在长日照下还是短日照下都没有明显的开花表型,说明COL7在调控拟南芥开化方面可能与其家族基因中的其它成员存在功能冗余.
