目的 探析Xα1型胶原蛋白(collagen type X alpha 1,COL10A1)、微小RNA(microRNA,miRNA)-21、miRNA-320c表达水平对胃癌腹膜转移及预后的预测效能。方法 回顾性分析内蒙古医科大学附属肿瘤医院2023年7月至2024年9月收治的120例胃癌患者...目的 探析Xα1型胶原蛋白(collagen type X alpha 1,COL10A1)、微小RNA(microRNA,miRNA)-21、miRNA-320c表达水平对胃癌腹膜转移及预后的预测效能。方法 回顾性分析内蒙古医科大学附属肿瘤医院2023年7月至2024年9月收治的120例胃癌患者的临床资料。治疗后采用门诊及电话随访形式随访患者预后结局,并根据是否发生腹膜转移分为腹膜转移组和无腹膜转移组;根据预后结局分为不良组和良好组。采用酶联免疫法检测COL10A1;采用qRT-PCR检测miRNA-21、miRNA-320c。对比腹膜转移组、无腹膜转移组临床特征、血清COL10A1、miRNA-21、miRNA-320c;对比不同预后胃癌患者血清COL10A1、miRNA-21、miRNA-320c表达水平;采用Cox单因素及多因素对胃癌预后影响因素进行逐步回归分析;采用ROC分析血清COL10A1、miRNA-21、miRNA-320c对胃癌预后的预测效能。结果 120例胃癌患者中,腹膜转移31例,无腹膜转移89例。腹膜转移组TNM分期高于无腹膜转移组(P<0.05)。腹膜转移组血清COL10A1、miRNA-21、miRNA-320c均高于无腹膜转移组(P<0.05)。120例胃癌患者中,不良组42例,良好组78例。不良组血清COL10A1、miRNA-21、miRNA-320c均高于良好组(P<0.05)。Cox单因素显示,TNM分期、腹膜转移、淋巴结转移、COL10A1、miRNA-21、miRNA-320c与胃癌患者预后相关(均P<0.05);多因素显示,TNM分期、腹膜转移、COL10A1、miRNA-21、miRNA-320c是影响胃癌患者预后的独立危险因素(均P<0.05)。ROC曲线显示,血清COL10A1、miRNA-21、miRNA-320c联合预测胃癌预后结局的灵敏度及特异度分别为89.45%、92.34%,AUC达0.921,高于COL10A1(P=0.011)、miRNA-21(P=0.023)及miRNA-320c(P=0.015)单独诊断。结论 血清COL10A1、miRNA-21、miRNA-320c在胃癌腹膜转移及不良预后患者中呈高表达水平,且上述指标为胃癌患者不良预后危险因素,联合应用对预后结局有着较高预测效能。展开更多
This study aimed to elucidate the role of collagen type XI alpha 1(COL11A1)-positive cancer-associated fibroblasts(CAFs)in modifying the tumor microenvironment of colon cancer(CC)and facilitating immune evasion throug...This study aimed to elucidate the role of collagen type XI alpha 1(COL11A1)-positive cancer-associated fibroblasts(CAFs)in modifying the tumor microenvironment of colon cancer(CC)and facilitating immune evasion through interactions with myeloid-derived suppressor cells(MDSCs).Using single-cell transcriptomic sequencing,we analyzed the interplay between COL11A1-positive CAFs and MDSCs in the CC microenvironment,focusing on how COL11A1 impacts MDSC differentiation and activation.The results demonstrate that COL11A1 expression in fibroblasts significantly enhances matrix metalloproteinase(MMP)3 and MMP13 expression,leading to paracrine induction of MDSC differentiation and activation,which promotes immune evasion and tumor growth.Additionally,we observed that COL11A1 knockout(COL11A1KO)suppresses tumor growth and hinders immune evasion.These findings underscore the essential role of COL11A1-positive CAFs in establishing an immunosuppressive tumor microenvironment conducive to CC progression.By elucidating the molecular pathway through which COL11A1 influences MDSC activity,this research suggests new therapeutic avenues for targeting the tumor microenvironment in CC,particularly through modulating COL11A1 expression in CAFs.展开更多
CONSTANS(CO)and CONSTANS-LIKE(COL)transcription factors are known to regulate a series of cellular processes,including the transition from vegetative growth to flower development in plants.However,their role in regula...CONSTANS(CO)and CONSTANS-LIKE(COL)transcription factors are known to regulate a series of cellular processes,including the transition from vegetative growth to flower development in plants.However,their role in regulating the fruit chlorophyll content is poorly understood.In this study,SlCOL1,the tomato(Solanum lycopersicum)ortholog of Arabidopsis CONSTANS,was shown to play key roles in controlling fruit chlorophyll.The suppression of SlCOL1 expression led to a reduction in the chlorophyll content of immature green fruit,while the overexpression of SlCOL1 increased it.An analysis of protein-protein interactions indicated that SlCOL1 forms a complex with GOLDEN2-LIKE(GLK2),which promotes the stability of its protein.The overexpression of SlCOL1in the glk2 null mutation background of tomato failed to promote chlorophyll accumulation in the immature green fruit,which suggests that GLK2 is required for the function of SlCOL1 in regulating chlorophyll content.These results shed new light on the mechanisms used by COL1 and GLK2 to regulate fruit development and chlorophyll accumulation in tomato.展开更多
Dermatofibrosarcoma protuberans(DFSP)is a rare cutaneous intermediate-grade soft tissue tumor characterized by COL1A1::PDGFB fusion in most cases.This fusion drives tumorigenesis and forms the basis for imatinib treat...Dermatofibrosarcoma protuberans(DFSP)is a rare cutaneous intermediate-grade soft tissue tumor characterized by COL1A1::PDGFB fusion in most cases.This fusion drives tumorigenesis and forms the basis for imatinib treatment,which acts by blocking platelet-derived growth factor receptor-beta kinase activity.Apart from this canonical fusion,there is an expanding spectrum of rare fusions,including COL6A3::PDGFD,EMILIN::PDGFD,TNC::PDGFD,etc.,through mole-cular profiling.These atypical rearrangements may be encountered in morpho-logically classic DFSP,unusual anatomic sites,or diagnostically challenging variants such as fibrosarcomatous DFSP.Their recognition is clinically relevant,as they may influence tumor biology,response to targeted therapy,and eligibility for clinical trials.This newly documented DFSP involving the lacrimal sac was initially misdiagnosed as a solitary fibrous tumor,emphasizing the diagnostic pitfalls in anatomically constrained regions and the importance of integrated diagnosis combining histology,immunohistochemistry,and molecular testing.In this editorial commentary,we briefly highlight the ever-growing genomic land-scape of DFSP,report rare fusions and their biological implications,and examine the role of expanded molecular diagnostics in refining diagnosis,guiding therapy,and informing prognosis.Incorporating comprehensive fusion analysis into routine workup may be critical for accurate classification,especially in unusual presentations where reliance on morphology alone risks misdiagnosis.展开更多
文摘目的 探析Xα1型胶原蛋白(collagen type X alpha 1,COL10A1)、微小RNA(microRNA,miRNA)-21、miRNA-320c表达水平对胃癌腹膜转移及预后的预测效能。方法 回顾性分析内蒙古医科大学附属肿瘤医院2023年7月至2024年9月收治的120例胃癌患者的临床资料。治疗后采用门诊及电话随访形式随访患者预后结局,并根据是否发生腹膜转移分为腹膜转移组和无腹膜转移组;根据预后结局分为不良组和良好组。采用酶联免疫法检测COL10A1;采用qRT-PCR检测miRNA-21、miRNA-320c。对比腹膜转移组、无腹膜转移组临床特征、血清COL10A1、miRNA-21、miRNA-320c;对比不同预后胃癌患者血清COL10A1、miRNA-21、miRNA-320c表达水平;采用Cox单因素及多因素对胃癌预后影响因素进行逐步回归分析;采用ROC分析血清COL10A1、miRNA-21、miRNA-320c对胃癌预后的预测效能。结果 120例胃癌患者中,腹膜转移31例,无腹膜转移89例。腹膜转移组TNM分期高于无腹膜转移组(P<0.05)。腹膜转移组血清COL10A1、miRNA-21、miRNA-320c均高于无腹膜转移组(P<0.05)。120例胃癌患者中,不良组42例,良好组78例。不良组血清COL10A1、miRNA-21、miRNA-320c均高于良好组(P<0.05)。Cox单因素显示,TNM分期、腹膜转移、淋巴结转移、COL10A1、miRNA-21、miRNA-320c与胃癌患者预后相关(均P<0.05);多因素显示,TNM分期、腹膜转移、COL10A1、miRNA-21、miRNA-320c是影响胃癌患者预后的独立危险因素(均P<0.05)。ROC曲线显示,血清COL10A1、miRNA-21、miRNA-320c联合预测胃癌预后结局的灵敏度及特异度分别为89.45%、92.34%,AUC达0.921,高于COL10A1(P=0.011)、miRNA-21(P=0.023)及miRNA-320c(P=0.015)单独诊断。结论 血清COL10A1、miRNA-21、miRNA-320c在胃癌腹膜转移及不良预后患者中呈高表达水平,且上述指标为胃癌患者不良预后危险因素,联合应用对预后结局有着较高预测效能。
文摘This study aimed to elucidate the role of collagen type XI alpha 1(COL11A1)-positive cancer-associated fibroblasts(CAFs)in modifying the tumor microenvironment of colon cancer(CC)and facilitating immune evasion through interactions with myeloid-derived suppressor cells(MDSCs).Using single-cell transcriptomic sequencing,we analyzed the interplay between COL11A1-positive CAFs and MDSCs in the CC microenvironment,focusing on how COL11A1 impacts MDSC differentiation and activation.The results demonstrate that COL11A1 expression in fibroblasts significantly enhances matrix metalloproteinase(MMP)3 and MMP13 expression,leading to paracrine induction of MDSC differentiation and activation,which promotes immune evasion and tumor growth.Additionally,we observed that COL11A1 knockout(COL11A1KO)suppresses tumor growth and hinders immune evasion.These findings underscore the essential role of COL11A1-positive CAFs in establishing an immunosuppressive tumor microenvironment conducive to CC progression.By elucidating the molecular pathway through which COL11A1 influences MDSC activity,this research suggests new therapeutic avenues for targeting the tumor microenvironment in CC,particularly through modulating COL11A1 expression in CAFs.
基金supported by grants from the National Natural Science Foundation of China(32360766,32072595 and 32202512)the Earmarked Fund for CARS(CARS-23-A13)。
文摘CONSTANS(CO)and CONSTANS-LIKE(COL)transcription factors are known to regulate a series of cellular processes,including the transition from vegetative growth to flower development in plants.However,their role in regulating the fruit chlorophyll content is poorly understood.In this study,SlCOL1,the tomato(Solanum lycopersicum)ortholog of Arabidopsis CONSTANS,was shown to play key roles in controlling fruit chlorophyll.The suppression of SlCOL1 expression led to a reduction in the chlorophyll content of immature green fruit,while the overexpression of SlCOL1 increased it.An analysis of protein-protein interactions indicated that SlCOL1 forms a complex with GOLDEN2-LIKE(GLK2),which promotes the stability of its protein.The overexpression of SlCOL1in the glk2 null mutation background of tomato failed to promote chlorophyll accumulation in the immature green fruit,which suggests that GLK2 is required for the function of SlCOL1 in regulating chlorophyll content.These results shed new light on the mechanisms used by COL1 and GLK2 to regulate fruit development and chlorophyll accumulation in tomato.
文摘Dermatofibrosarcoma protuberans(DFSP)is a rare cutaneous intermediate-grade soft tissue tumor characterized by COL1A1::PDGFB fusion in most cases.This fusion drives tumorigenesis and forms the basis for imatinib treatment,which acts by blocking platelet-derived growth factor receptor-beta kinase activity.Apart from this canonical fusion,there is an expanding spectrum of rare fusions,including COL6A3::PDGFD,EMILIN::PDGFD,TNC::PDGFD,etc.,through mole-cular profiling.These atypical rearrangements may be encountered in morpho-logically classic DFSP,unusual anatomic sites,or diagnostically challenging variants such as fibrosarcomatous DFSP.Their recognition is clinically relevant,as they may influence tumor biology,response to targeted therapy,and eligibility for clinical trials.This newly documented DFSP involving the lacrimal sac was initially misdiagnosed as a solitary fibrous tumor,emphasizing the diagnostic pitfalls in anatomically constrained regions and the importance of integrated diagnosis combining histology,immunohistochemistry,and molecular testing.In this editorial commentary,we briefly highlight the ever-growing genomic land-scape of DFSP,report rare fusions and their biological implications,and examine the role of expanded molecular diagnostics in refining diagnosis,guiding therapy,and informing prognosis.Incorporating comprehensive fusion analysis into routine workup may be critical for accurate classification,especially in unusual presentations where reliance on morphology alone risks misdiagnosis.
