Background Baicalin and probiotic cocktails are promising feed additives with broad application prospects.While probiotic cocktails are known to enhance intestinal health,the potential synergistic impact of combining ...Background Baicalin and probiotic cocktails are promising feed additives with broad application prospects.While probiotic cocktails are known to enhance intestinal health,the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains largely unexplored.Therefore,this study aims to investigate the influence of the combined administration of baicalin and probiotic cocktails on the composition of ileal and cecal microbiota in broiler chickens to elucidate the underlying mechanisms responsible for the healthpromoting effects.Results A total of 3201-day-old male Arbor Acres broilers were divided into 4 groups,each with 8 replicates of 10 chicks per replicate.Over a period of 42 d,the birds were fed a basal diet or the same diet supplemented with 37.5 g/t baicalin(BC),1,000 g/t probiotic cocktails(PC),or a combination of both BC(37.5 g/t)and PC(1,000 g/t).The results demonstrated that BC+PC exhibited positive synergistic effects,enhancing intestinal morphology,immune function,and barrier function.This was evidenced by increased VH/CD ratio,sIgA levels,and upregulated expression of occludin and claudin-1(P<0.05).16S rRNA analysis indicated that PC potentiated the effects of BC,particularly in the ileum,where BC+PC significantly increased theα-diversity of the ileal microbiota,altered itsβ-diversity,and increased the relative abundance of Flavonifractor(P<0.05),a flavonoid-metabolizing bacterium.Furthermore,Flavonifractor positively correlated with chicken ileum crypt depth(P<0.05).While BC+PC had a limited effect on cecal microbiota structure,the PC group had a very similar microbial composition to BC+PC,suggesting that the effect of PC at the distal end of the gut overshadowed those of BC.Conclusions We demonstrated the synergistic enhancement of gut health regulation in broiler chickens by combining baicalin and probiotic cocktails.Probiotic cocktails enhanced the effects of baicalin and accelerated its metabolism in the ileum,thereby influencing the ileal microbiota structure.This study elucidates the interaction mechanism between probiotic cocktails and plant extract additives within the host microbiota.These findings provide compelling evidence for the future development of feed additive combinations.展开更多
[Objective]To investigate the effect of probiotic cocktails on growth performance,intestinal barrier function and antioxidant status in heat-stressed broilers.[Methods]One thousand 1-day-old broilers were randomly div...[Objective]To investigate the effect of probiotic cocktails on growth performance,intestinal barrier function and antioxidant status in heat-stressed broilers.[Methods]One thousand 1-day-old broilers were randomly divided into five groups:the thermoneutral(TN),heat stress(HS),heat stress with antibiotic(HS-AB),heat stress with probiotic cocktail 1(HS-P1),and heat stress with probiotic cocktail 2(HS-P2)groups.The broilers in TN and HS groups received a diet without supplements,and those in HS-AB,HS-P1 and HS-P2 groups were fed a diet supplemented with chlortetracycline(300 g/t)or probiotic cocktail 1 or 2(100 g/t),respectively.[Results]The results showed that heat stress reduced growth performance and increased inflammatory factors,NF-κB and Toll-like receptors in jejunal mucosa coinciding with higher expression of jejunal tight junction proteins,mucin and heat stress protein mRNA.[Conclusion]Dietary probiotic cocktails had protective effects against the heat stress-induced changes in the intestine,with increased serum antioxidant capacity and growth performance of broilers.The effect of these cocktails was similar to that of chlortetracycline.展开更多
Perinatal brain injury（PBI）is one of the most important causes of lifelong deficits in cognition,behavior,social interaction and motor skills,as well as epilepsy.PBI encompasses,but is not limited to,hypoxic-ischemi...Perinatal brain injury（PBI）is one of the most important causes of lifelong deficits in cognition,behavior,social interaction and motor skills,as well as epilepsy.PBI encompasses,but is not limited to,hypoxic-ischemic encephalopathy（HIE）,intraventricular hemorrhage,periventricular leukomalacia and encephalopathy of prematurity.展开更多
Adoptive immune cell-based therapies have shown promise in cancer treatment,yet their efficacy against solid tumors is often limited by the immunosuppressive tumor microenvironment(TME).To overcome these barriers,we d...Adoptive immune cell-based therapies have shown promise in cancer treatment,yet their efficacy against solid tumors is often limited by the immunosuppressive tumor microenvironment(TME).To overcome these barriers,we design an innovative immune cell cocktail as a combinatorial biomaterial platform,which harnessing the complementary functions of neutrophils and natural killer(NK)cells derived from human pluripotent stem cells(hPSCs).Using CRISPR/Cas9,we introduce an anti-fluorescein isothiocyanate(FITC)chimeric antigen receptor(CAR)construct into the AAVS1 safe harbor locus of hPSCs,allowing for the differentiation of CAR-modified neutrophils and NK cells.These CAR neutrophils exhibit robust anti-tumor activity,forming immune synapses with tumor cells tagged via a bispecific adapter(FITC-folate),even in hypoxic TMEs,while CAR NK cells demonstrate antigen-specific cytotoxicity.Together,the cocktail biomaterial composed of CAR neutrophils and CAR NK cells creates a synergistic anti-tumor effect:having neutrophils enhance TME modulation,and NK cells provide targeted cytotoxicity.This biomaterial offers a scalable and off-the-shelf solution for producing CAR neutrophils and CAR NK cells,potentially reducing needs for high-dose exogenous cytokines and minimizing immune-related toxicities.Our findings suggest that hPSC-derived CAR neutrophils and CAR NK cells may form an effective immuno-cocktail biomaterial,offering a feasible strategy for advancing solid tumor immunotherapy through cellular synergy and TME adaptation.展开更多
L ocated in one of Shanghafs new designdriven hotels,The Sukhothai Shanghai,The ZUK Bar is one of the most ambitious hotel cocktail bars that we have come across.First of all,they ve recruited Vicenzo Pagliara,formerl...L ocated in one of Shanghafs new designdriven hotels,The Sukhothai Shanghai,The ZUK Bar is one of the most ambitious hotel cocktail bars that we have come across.First of all,they ve recruited Vicenzo Pagliara,formerly of Londons well-regarded The Connaught Bai;as their head of mixology.展开更多
If youve been to Bar No.3,you might experience a sense of deja vu at this hip addition to the Oha empire.The design firm turned food and beverage group has installed a bar at Dead Poet that resembles its predecessor B...If youve been to Bar No.3,you might experience a sense of deja vu at this hip addition to the Oha empire.The design firm turned food and beverage group has installed a bar at Dead Poet that resembles its predecessor Bar No.3 with the notable exception of a very fancy looking sound system on the well-stocked shelves.展开更多
基金funded by National Key R&D Program of China(2022YFD1300403,2021YFD1300404)China Agriculture Research System program(CARS-40,CARS-41-G11)Beijing Natural Science Foundation(6222036).
文摘Background Baicalin and probiotic cocktails are promising feed additives with broad application prospects.While probiotic cocktails are known to enhance intestinal health,the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains largely unexplored.Therefore,this study aims to investigate the influence of the combined administration of baicalin and probiotic cocktails on the composition of ileal and cecal microbiota in broiler chickens to elucidate the underlying mechanisms responsible for the healthpromoting effects.Results A total of 3201-day-old male Arbor Acres broilers were divided into 4 groups,each with 8 replicates of 10 chicks per replicate.Over a period of 42 d,the birds were fed a basal diet or the same diet supplemented with 37.5 g/t baicalin(BC),1,000 g/t probiotic cocktails(PC),or a combination of both BC(37.5 g/t)and PC(1,000 g/t).The results demonstrated that BC+PC exhibited positive synergistic effects,enhancing intestinal morphology,immune function,and barrier function.This was evidenced by increased VH/CD ratio,sIgA levels,and upregulated expression of occludin and claudin-1(P<0.05).16S rRNA analysis indicated that PC potentiated the effects of BC,particularly in the ileum,where BC+PC significantly increased theα-diversity of the ileal microbiota,altered itsβ-diversity,and increased the relative abundance of Flavonifractor(P<0.05),a flavonoid-metabolizing bacterium.Furthermore,Flavonifractor positively correlated with chicken ileum crypt depth(P<0.05).While BC+PC had a limited effect on cecal microbiota structure,the PC group had a very similar microbial composition to BC+PC,suggesting that the effect of PC at the distal end of the gut overshadowed those of BC.Conclusions We demonstrated the synergistic enhancement of gut health regulation in broiler chickens by combining baicalin and probiotic cocktails.Probiotic cocktails enhanced the effects of baicalin and accelerated its metabolism in the ileum,thereby influencing the ileal microbiota structure.This study elucidates the interaction mechanism between probiotic cocktails and plant extract additives within the host microbiota.These findings provide compelling evidence for the future development of feed additive combinations.
文摘[Objective]To investigate the effect of probiotic cocktails on growth performance,intestinal barrier function and antioxidant status in heat-stressed broilers.[Methods]One thousand 1-day-old broilers were randomly divided into five groups:the thermoneutral(TN),heat stress(HS),heat stress with antibiotic(HS-AB),heat stress with probiotic cocktail 1(HS-P1),and heat stress with probiotic cocktail 2(HS-P2)groups.The broilers in TN and HS groups received a diet without supplements,and those in HS-AB,HS-P1 and HS-P2 groups were fed a diet supplemented with chlortetracycline(300 g/t)or probiotic cocktail 1 or 2(100 g/t),respectively.[Results]The results showed that heat stress reduced growth performance and increased inflammatory factors,NF-κB and Toll-like receptors in jejunal mucosa coinciding with higher expression of jejunal tight junction proteins,mucin and heat stress protein mRNA.[Conclusion]Dietary probiotic cocktails had protective effects against the heat stress-induced changes in the intestine,with increased serum antioxidant capacity and growth performance of broilers.The effect of these cocktails was similar to that of chlortetracycline.
文摘Perinatal brain injury（PBI）is one of the most important causes of lifelong deficits in cognition,behavior,social interaction and motor skills,as well as epilepsy.PBI encompasses,but is not limited to,hypoxic-ischemic encephalopathy（HIE）,intraventricular hemorrhage,periventricular leukomalacia and encephalopathy of prematurity.
基金supported by start-up package funding from The Hong Kong Polytechnic University(Y.C.)and the Shenzhen Science and Technology Program(No.JCYJ20241202130520027).
文摘Adoptive immune cell-based therapies have shown promise in cancer treatment,yet their efficacy against solid tumors is often limited by the immunosuppressive tumor microenvironment(TME).To overcome these barriers,we design an innovative immune cell cocktail as a combinatorial biomaterial platform,which harnessing the complementary functions of neutrophils and natural killer(NK)cells derived from human pluripotent stem cells(hPSCs).Using CRISPR/Cas9,we introduce an anti-fluorescein isothiocyanate(FITC)chimeric antigen receptor(CAR)construct into the AAVS1 safe harbor locus of hPSCs,allowing for the differentiation of CAR-modified neutrophils and NK cells.These CAR neutrophils exhibit robust anti-tumor activity,forming immune synapses with tumor cells tagged via a bispecific adapter(FITC-folate),even in hypoxic TMEs,while CAR NK cells demonstrate antigen-specific cytotoxicity.Together,the cocktail biomaterial composed of CAR neutrophils and CAR NK cells creates a synergistic anti-tumor effect:having neutrophils enhance TME modulation,and NK cells provide targeted cytotoxicity.This biomaterial offers a scalable and off-the-shelf solution for producing CAR neutrophils and CAR NK cells,potentially reducing needs for high-dose exogenous cytokines and minimizing immune-related toxicities.Our findings suggest that hPSC-derived CAR neutrophils and CAR NK cells may form an effective immuno-cocktail biomaterial,offering a feasible strategy for advancing solid tumor immunotherapy through cellular synergy and TME adaptation.
文摘L ocated in one of Shanghafs new designdriven hotels,The Sukhothai Shanghai,The ZUK Bar is one of the most ambitious hotel cocktail bars that we have come across.First of all,they ve recruited Vicenzo Pagliara,formerly of Londons well-regarded The Connaught Bai;as their head of mixology.
文摘If youve been to Bar No.3,you might experience a sense of deja vu at this hip addition to the Oha empire.The design firm turned food and beverage group has installed a bar at Dead Poet that resembles its predecessor Bar No.3 with the notable exception of a very fancy looking sound system on the well-stocked shelves.
