The yeast Saccharomyces boulardii CNCM I-745 is one of the probiotics recommended for the prevention of antibiotic-associated diarrhea. Studies conducted in vivo and in vitro demonstrated that in the case of infectiou...The yeast Saccharomyces boulardii CNCM I-745 is one of the probiotics recommended for the prevention of antibiotic-associated diarrhea. Studies conducted in vivo and in vitro demonstrated that in the case of infectious diseases there are two potential sites of action of Saccharomyces boulardii CNCM I-745:(1)An action on enteropathogenic microorganisms(adhesion of bacteria and their elimination or an effect on their virulence factors: Toxins, lipopolysaccharide,etc.); and(2) a direct action on the intestinal mucosa(trophic effects, effects on epithelial reconstitution, anti-secretory effects, anti-inflammatory,immunomodulators). Oral administration of Saccharomyces boulardii CNCM I-745 to healthy subjects does not alter their microbiota. However, in the case of diseases associated with the use of antibiotics or chronic diarrhea, Saccharomyces boulardii CNCM I-745 can restore the intestinal microbiota faster. The interaction of Saccharomyces boulardii CNCM I-745 with the innate immune system have been recently demonstrated thus opening up a new therapeutic potential of this yeast in the case of diseases associated with intestinal infections but also other pathologies associated with dysbiosis such as inflammatory diseases.展开更多
Coffee mucilage(CM),a by-product of grain-processing,offers a sustainable and innovative alternative as a wall material for food-grade microencapsulation.Its potential in probiotic delivery remains underexplored,espec...Coffee mucilage(CM),a by-product of grain-processing,offers a sustainable and innovative alternative as a wall material for food-grade microencapsulation.Its potential in probiotic delivery remains underexplored,especially in combination with established carriers such as inulin(INU).This study aimed to develop microcapsules containing Saccharomyces boulardii CNCM I-745 using spray drying(SD),employing CM,INU,and a CM/INU blend as wall materials.Three formulations—SD-CM,SD-INU,and SD-CM/INU—were produced and assessed for encapsulation yield(EY);structural properties(Fourier transform infrared spectroscopy(FTIR)and Zeta(ζ)potential),morphological properties(scanning electron microscopy(SEM),particle size(PS)distribution,and polydispersity index(PI))and thermal properties(differential scanning calorimetry and thermogravimetric analysis(DSC/TGA))as well as and bioactive attributes(total phenolic content(TPC)expressed as milligrams of gallic acid equivalents per gram of sample(mg GAE/g)and antioxidant activity).FTIR analysis confirmed encapsulation through hydrogen bonding between probiotic amide groups and polysaccharide hydroxyl groups.SEM revealed that SD-INU microcapsules exhibited smooth,spherical surfaces with minimal agglomeration,whereas SD-CM and SD-CM/INU showed irregular morphologies.Mean PS were 2.75±0.92μm(SD-CM),2.27±0.67μm(SD-INU),and 2.51±0.99μm(SD-CM/INU),with PI indicating greater homogeneity in SD-INU.SDINU also demonstrated superior colloidal stability(-10.65±1.07 mV)compared to SD-CM(-9.39±0.11 mV).Notably,SD-CM/INU microcapsules exhibited the highest thermal stability(glass transition temperature,Tg=69.56℃).SD-CM retained the highest TPC of 1.236±0.037(mg GAE/g)and antioxidant activity(69.30±6.62%inhibiton of 2,2-diphenyl-1-picrylhydrazyl radical(DPPH^(•))),attributed to CM-derived bioactive compounds.Overall,CM and INU-based wall materials—particularly in combination—show promise for the development of thermally stable,bioactive-rich microcapsules of Saccharomyces boulardii,with potential applications in functional food systems.展开更多
文摘The yeast Saccharomyces boulardii CNCM I-745 is one of the probiotics recommended for the prevention of antibiotic-associated diarrhea. Studies conducted in vivo and in vitro demonstrated that in the case of infectious diseases there are two potential sites of action of Saccharomyces boulardii CNCM I-745:(1)An action on enteropathogenic microorganisms(adhesion of bacteria and their elimination or an effect on their virulence factors: Toxins, lipopolysaccharide,etc.); and(2) a direct action on the intestinal mucosa(trophic effects, effects on epithelial reconstitution, anti-secretory effects, anti-inflammatory,immunomodulators). Oral administration of Saccharomyces boulardii CNCM I-745 to healthy subjects does not alter their microbiota. However, in the case of diseases associated with the use of antibiotics or chronic diarrhea, Saccharomyces boulardii CNCM I-745 can restore the intestinal microbiota faster. The interaction of Saccharomyces boulardii CNCM I-745 with the innate immune system have been recently demonstrated thus opening up a new therapeutic potential of this yeast in the case of diseases associated with intestinal infections but also other pathologies associated with dysbiosis such as inflammatory diseases.
基金supported by FONDO NACIONAL DE FINANCIA-MIENTO PARA LA CIENCIA,LA TECNOLOGIA Y LA INNOVACIÓN FRANCISCO JOSE DE CALDAS[PROYECTO 109065].
文摘Coffee mucilage(CM),a by-product of grain-processing,offers a sustainable and innovative alternative as a wall material for food-grade microencapsulation.Its potential in probiotic delivery remains underexplored,especially in combination with established carriers such as inulin(INU).This study aimed to develop microcapsules containing Saccharomyces boulardii CNCM I-745 using spray drying(SD),employing CM,INU,and a CM/INU blend as wall materials.Three formulations—SD-CM,SD-INU,and SD-CM/INU—were produced and assessed for encapsulation yield(EY);structural properties(Fourier transform infrared spectroscopy(FTIR)and Zeta(ζ)potential),morphological properties(scanning electron microscopy(SEM),particle size(PS)distribution,and polydispersity index(PI))and thermal properties(differential scanning calorimetry and thermogravimetric analysis(DSC/TGA))as well as and bioactive attributes(total phenolic content(TPC)expressed as milligrams of gallic acid equivalents per gram of sample(mg GAE/g)and antioxidant activity).FTIR analysis confirmed encapsulation through hydrogen bonding between probiotic amide groups and polysaccharide hydroxyl groups.SEM revealed that SD-INU microcapsules exhibited smooth,spherical surfaces with minimal agglomeration,whereas SD-CM and SD-CM/INU showed irregular morphologies.Mean PS were 2.75±0.92μm(SD-CM),2.27±0.67μm(SD-INU),and 2.51±0.99μm(SD-CM/INU),with PI indicating greater homogeneity in SD-INU.SDINU also demonstrated superior colloidal stability(-10.65±1.07 mV)compared to SD-CM(-9.39±0.11 mV).Notably,SD-CM/INU microcapsules exhibited the highest thermal stability(glass transition temperature,Tg=69.56℃).SD-CM retained the highest TPC of 1.236±0.037(mg GAE/g)and antioxidant activity(69.30±6.62%inhibiton of 2,2-diphenyl-1-picrylhydrazyl radical(DPPH^(•))),attributed to CM-derived bioactive compounds.Overall,CM and INU-based wall materials—particularly in combination—show promise for the development of thermally stable,bioactive-rich microcapsules of Saccharomyces boulardii,with potential applications in functional food systems.
