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Investigation on the Sterility Changeover of Male Sterility Line CMS7311 in Heading Chinese Cabbage 被引量:12
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作者 张鲁刚 郝东方 《Acta Botanica Sinica》 CSCD 2001年第11期1123-1128,共6页
Sterility changeover induced by low temperature in male sterility line CMS7311 of heading Chinese cabbage ( Brassica campestris L. ssp. penkinsis) was investigated under constant temperature, day/night alternating tem... Sterility changeover induced by low temperature in male sterility line CMS7311 of heading Chinese cabbage ( Brassica campestris L. ssp. penkinsis) was investigated under constant temperature, day/night alternating temperature in light-growth-incubator and outdoor natural day/night alternating temperature respectively. It showed that the sterility of CMS7311 has significant change with temperature. The constant temperature of 6 - 12 degreesC was suitable to induce the changeover of sterility (CGS) of CMS7311 ; the shortest treatment time for CGS induction at this temperature was 3 d. The significant value of sterility changeover of CMS7311 occurred at the constant temperature of 6 - 9 degreesC after treatment for 6 - 9 d. The intensity of CGS was stronger under constant temperature than under alternate temperature, but the shortest treatment time inducing CGS has no difference between them. When treated for 9 d under the day/night alternating temperature of 11/7 degreesC, its sterility could also turn to normal fertility (near to sterile grade I or zero). In normal condition, the interval from the end of temperature treatment to the beginning of CGS induction was 10 - 16 d. In the condition of natural day/night alternating temperature, the lower limit of temperature for effective CGS induction was at the daily mean temperature ( DMT) of 3. 5 - 6. 8 degreesC, and the shortest treatment time which could induce the sterility changeover was also longer than that under constant temperature, being 11d at DMT of 1.5 - 3.1 degreesC. In addition, for the apical dominance, the flower bud on the first lateral branch showed the CGS 8 - 10 d later than the flower bud on the main stem under the same temperature with the same days of treatment. The intensity of CGS of CMS7311 on the first lateral branch was also weaker than the flower bud on the main stem. 展开更多
关键词 heading Chinese cabbage cms7311 changeover of sterility (CGS) temperature sensitive
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大白菜胞质雄性不育系育性相关线粒体DNA片断的克隆及*序列分析 被引量:7
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作者 张战凤 张鲁刚 +2 位作者 王绮 惠麦侠 张明科 《西北农业学报》 CAS CSCD 北大核心 2006年第3期112-115,共4页
利用PCR技术从大白菜(Brassica campestrisL.ssp.Penkinsis)新型胞质雄性不育材料CMS7311的线粒体基因组中扩增出与细胞质雄性不育相关的片断,并克隆测序。序列分析表明,该片断和报道的Poli-ma-orf224序列完全相同,没有发生变异,说明CMS... 利用PCR技术从大白菜(Brassica campestrisL.ssp.Penkinsis)新型胞质雄性不育材料CMS7311的线粒体基因组中扩增出与细胞质雄性不育相关的片断,并克隆测序。序列分析表明,该片断和报道的Poli-ma-orf224序列完全相同,没有发生变异,说明CMS7311的不育机理与PolimaCMS相似,也反映了细胞质雄性不育变异的保守性。 展开更多
关键词 大白菜 cms7311 细胞质雄性不育(CMS) 线粒体DNA(mtDNA) orf224
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橙色大白菜遗传转化体系的初步建立 被引量:2
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作者 范爱丽 张鲁刚 《长江蔬菜》 2012年第18期10-14,共5页
以06J28橙色大白菜子叶段为外植体,通过根癌农杆菌介导CMS7311-orf224基因,探讨了潮霉素、头孢霉素、预培养时间、农杆菌浓度、感菌时间和共培养时间等因素对橙色大白菜遗传转化的影响。试验结果表明,子叶段预培养2~3 d后,在OD600值为0.... 以06J28橙色大白菜子叶段为外植体,通过根癌农杆菌介导CMS7311-orf224基因,探讨了潮霉素、头孢霉素、预培养时间、农杆菌浓度、感菌时间和共培养时间等因素对橙色大白菜遗传转化的影响。试验结果表明,子叶段预培养2~3 d后,在OD600值为0.3~0.5的农杆菌EHA-105菌液中侵染5 min,再共培养2~3 d,在培养基中加入5 mg/LHyg(抗性筛选)和500 mg/L Cef(脱菌)可得到转化植株。试验初步建立了橙色大白菜遗传转化体系,为大白菜种质资源创新奠定了基础。 展开更多
关键词 橙色大白菜 cms7311-orf224基因 遗传转化
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