The three-line hybrid rice system,which relies on cytoplasmic male sterility(CMS),faces challenges in breeding efficiency.To address these challenges,we developed CMS engineering breeding technology(CEBT).First,we con...The three-line hybrid rice system,which relies on cytoplasmic male sterility(CMS),faces challenges in breeding efficiency.To address these challenges,we developed CMS engineering breeding technology(CEBT).First,we constructed a complementation vector containing the fertility restoration module Double Rf4-Rf20,sorting marker gene DsRed2,and pollen lethal gene ZmAA1,then transformed this into a CMS line.We obtained reproductive lines without alterations to main agronomic traits,which can produce engineering sterile lines(ESL)and reproductive line seeds at a 1:1 ratio by self-crossing.CEBT converts the traditional three-line system into an efficient‘new two-line’hybrid framework:the reproductive line is improved while simultaneously improving the ESL,which significantly shortens the breeding process for three-line hybrid rice.展开更多
基金supported by grants from by the Biological Breeding-National Science and Technology Major Project(2023ZD04076)the Science and Technology Innovation Program of Hunan Province(2024RC1070)+2 种基金the Hunan Natural Science Foundation(2023JJ30442)Joint Funds of the National Natural Science Foundation of China(U24A20396)Special Funds for the Construction of Innovative Provinces in Hunan Province(2021NK1011)。
文摘The three-line hybrid rice system,which relies on cytoplasmic male sterility(CMS),faces challenges in breeding efficiency.To address these challenges,we developed CMS engineering breeding technology(CEBT).First,we constructed a complementation vector containing the fertility restoration module Double Rf4-Rf20,sorting marker gene DsRed2,and pollen lethal gene ZmAA1,then transformed this into a CMS line.We obtained reproductive lines without alterations to main agronomic traits,which can produce engineering sterile lines(ESL)and reproductive line seeds at a 1:1 ratio by self-crossing.CEBT converts the traditional three-line system into an efficient‘new two-line’hybrid framework:the reproductive line is improved while simultaneously improving the ESL,which significantly shortens the breeding process for three-line hybrid rice.
