Nitrogen(N)is a key component in plants and their biological macromolecules,having a profound effect on developmental stages,such as germination,vegetative growth,and flowering.However,the mechanism of nitrogen-regula...Nitrogen(N)is a key component in plants and their biological macromolecules,having a profound effect on developmental stages,such as germination,vegetative growth,and flowering.However,the mechanism of nitrogen-regulated flowering time remains unclear.In this study,CmNLP7 was isolated from the chrysanthemum cultivar‘Jinba'and was characterized.CmNLP7 is a transcription factor localized in the nucleus but has no transcriptional activity.Tissue expression pattern analysis showed that CmNLP7 was mainly transcribed in leaves and roots.Knocking down CmNLP7 through the artificial-miRNA method in chrysanthemum resulted in early flowering under optimal nitrogen(ON)and low nitrogen(LN)conditions;whereas overexpression lines showed delayed flowering under LN conditions.Transcriptome sequencing analysis showed that the nitrate transporters NRT2.5,NPF3.1,and NPF4.6;SBP-like genes SPL7 and SPL12,and flowering integration factor FT were significantly up-regulated in the knockdown lines.Based on the KEGG pathway enrichment analysis,the differentially transcribed genes were enriched in phenylpropanoid biosynthesis and starch and sucrose metabolism pathways,which indicated their alleged function in nitrogen-regulated flowering and development in chrysanthemum.Furthermore CmPP6 as a homolog of the Arabidopsis phosphatase PP6,was verified as an interacting protein of CmNLP7 by yeast two-hybrid,BiFC,pull-down and Biacore in vitro and in vivo,and the knockdown line of CmPP6(amiR-CmPP6)flowered earlier compared to that of the wild-type chrysanthemum‘Jinba'.Collectively,these results demonstrated that CmPP6 interacts with CmNLP7 to regulate chrysanthemum flowering,and CmNLP7 could regulate flowering time in response to nitrogen,which lays a foundation for the regulation of flowering and molecular breeding of chrysanthemum through changes in nutrient signaling.展开更多
Relying on CMPP (China Mobile Peer to Peer) protocol,we propose and design SMS (Short Message Service) gateway interface for early warning plan based on real-time meteorological database application,in order to form t...Relying on CMPP (China Mobile Peer to Peer) protocol,we propose and design SMS (Short Message Service) gateway interface for early warning plan based on real-time meteorological database application,in order to form the meteorological mobile internet service system which is 'One point connect,service the whole province' for short.Accessing interface system to each city's SMG (Short Message Gateway) through standard protocol,we establish the information transmitting channel of short message platform and mobile SMG to realize the store-forward and flow control of short message.In addition,the stable and dependable communication connect of interface system and mobile SMG should be ensure,and the connect could be reconstructed while encountering any error,as well as committing short message would be stopped due to interruption of connect.展开更多
基金supported by grants from the National Natural Science Foundation of China(Grant No.31930100)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions。
文摘Nitrogen(N)is a key component in plants and their biological macromolecules,having a profound effect on developmental stages,such as germination,vegetative growth,and flowering.However,the mechanism of nitrogen-regulated flowering time remains unclear.In this study,CmNLP7 was isolated from the chrysanthemum cultivar‘Jinba'and was characterized.CmNLP7 is a transcription factor localized in the nucleus but has no transcriptional activity.Tissue expression pattern analysis showed that CmNLP7 was mainly transcribed in leaves and roots.Knocking down CmNLP7 through the artificial-miRNA method in chrysanthemum resulted in early flowering under optimal nitrogen(ON)and low nitrogen(LN)conditions;whereas overexpression lines showed delayed flowering under LN conditions.Transcriptome sequencing analysis showed that the nitrate transporters NRT2.5,NPF3.1,and NPF4.6;SBP-like genes SPL7 and SPL12,and flowering integration factor FT were significantly up-regulated in the knockdown lines.Based on the KEGG pathway enrichment analysis,the differentially transcribed genes were enriched in phenylpropanoid biosynthesis and starch and sucrose metabolism pathways,which indicated their alleged function in nitrogen-regulated flowering and development in chrysanthemum.Furthermore CmPP6 as a homolog of the Arabidopsis phosphatase PP6,was verified as an interacting protein of CmNLP7 by yeast two-hybrid,BiFC,pull-down and Biacore in vitro and in vivo,and the knockdown line of CmPP6(amiR-CmPP6)flowered earlier compared to that of the wild-type chrysanthemum‘Jinba'.Collectively,these results demonstrated that CmPP6 interacts with CmNLP7 to regulate chrysanthemum flowering,and CmNLP7 could regulate flowering time in response to nitrogen,which lays a foundation for the regulation of flowering and molecular breeding of chrysanthemum through changes in nutrient signaling.
文摘Relying on CMPP (China Mobile Peer to Peer) protocol,we propose and design SMS (Short Message Service) gateway interface for early warning plan based on real-time meteorological database application,in order to form the meteorological mobile internet service system which is 'One point connect,service the whole province' for short.Accessing interface system to each city's SMG (Short Message Gateway) through standard protocol,we establish the information transmitting channel of short message platform and mobile SMG to realize the store-forward and flow control of short message.In addition,the stable and dependable communication connect of interface system and mobile SMG should be ensure,and the connect could be reconstructed while encountering any error,as well as committing short message would be stopped due to interruption of connect.
