Calmodulin-like (CMLs) proteins are critical in calcium signaling and essential for plant growth, development, and stress responses. In many species, the CMLs families have been identified and described. However, the ...Calmodulin-like (CMLs) proteins are critical in calcium signaling and essential for plant growth, development, and stress responses. In many species, the CMLs families have been identified and described. However, the characterization and expression profiling of CMLs genes in tobacco is retrievable. In this study, a comprehensive whole-genome identification and analysis, and 75 NtCML genes were identified in tobacco, each containing two to four EF-hand domains. Most NtCML proteins exhibited conserved gene structures and motifs. Notably, most NtCML proteins were intron-less and distributed across 18 chromosomes. Two pairs of tandemly duplicated genes and seven pairs of segmentally duplicated genes were identified within the tobacco genome. Furthermore, 22 pairs of orthologous CMLs genes were discovered between Arabidopsis and tobacco. Cis-acting element analysis revealed that elements associated with hormones, stress responses, and plant growth and development were found in the promoter regions. Expression analysis indicated that some NtCML genes displayed tissue-specific expression patterns. Specifically, NtCML12, NtCML18, NtCML27, and NtCML28 showed significant upregulation during cold acclimation treatment. These results indicate that tobacco CMLs act as Ca^(2+) signal transducers, regulating plant growth and abiotic stress responses.展开更多
【目的】鉴定青海茄参(Mandragora chinghaiensis Kuang et A.M.Jl)CML基因家族成员,探究其对冷胁迫的响应。【方法】基于课题组的全长转录组数据,利用生物信息学技术对青海茄参CML基因家族的成员及其蛋白理化性质、系统发育过程、基因...【目的】鉴定青海茄参(Mandragora chinghaiensis Kuang et A.M.Jl)CML基因家族成员,探究其对冷胁迫的响应。【方法】基于课题组的全长转录组数据,利用生物信息学技术对青海茄参CML基因家族的成员及其蛋白理化性质、系统发育过程、基因结构等进行分析。【结果】在青海茄参中共鉴定出19个CML基因家族成员,亚细胞定位主要在细胞核,含有1~4个EF-hand结构域,二三级结构主要由α-螺旋组成;基序分析发现,McCMLs蛋白含有4个典型的EF手部基序;系统进化树分析显示,McCMLs蛋白被分为3个亚族。冷胁迫表达模式分析表明,McCML基因家族在冷胁迫下差异表达,其中McCML3、McCML7表达量显著上调。【结论】通过对McCML基因家族进行鉴定和分析,筛选出McCML3、McCML7作为青海茄参抗寒候选基因,为后期研究其抗寒分子机制提供理论基础。展开更多
Calcium(Ca^(2+)), a crucial second messenger in plants, is involved in diverse signaling pathways including biotic and abiotic stress responses. However, the functions of most calcium sensors including calcium-depende...Calcium(Ca^(2+)), a crucial second messenger in plants, is involved in diverse signaling pathways including biotic and abiotic stress responses. However, the functions of most calcium sensors including calcium-dependent protein kinases(CDPKs), calmodulins(Ca Ms), calmodulinlike proteins(CMLs), and calcineurin B-like proteins(CBLs) remain limited in plants, particularly in woody plants. Currently, a total of 83 CMLs and seven Ca Ms were discovered in the apple genome in this study. Functional domain analysis showed that these CMLs and Ca Ms contain a highly conserved EF-hand motif. q RT-PCR assays indicated that these CMLs were expressed ubiquitously in apple, including in the roots, stems,leaves, flowers, and fruits, and also possessed spatial specificity. Finally, most of these CMLs were induced by hormonal and abiotic stress, suggesting their potential roles in the regulation of growth, development, and the stress response in apple. In summary, our findings provide potential evidence that apple CMLs may be involved in abiotic stress and the regulation of plant growth and development.展开更多
Background: Herbal medicine is well-known among the ancient medical sciences. Healing properties have been observed in some species of Daphne plant. The effect of Daphne plant extract on the K562 cell line has been pr...Background: Herbal medicine is well-known among the ancient medical sciences. Healing properties have been observed in some species of Daphne plant. The effect of Daphne plant extract on the K562 cell line has been previously studied, and Gleevec is a well-known and effective medicine for the treatment of chronic myelogenous leukemia. Material and Methods: In this study, the simultaneous effects of using herbal medicine and a target therapy medicine on the K562 cell line were investigated. The presence of some species of Daphne in Iran motivated us to evaluate the cytotoxic effect of Daphne mucronata on human leukemia cancer cells. The antiproliferative activity of the dichloromethane extract of Daphne mucronate (Thymelaeaceae), a new anticancer medicinal plant, was evaluated. Cell viability was quantitated by MTT assay. Apoptotic and necrotic changes in the cell membrane were examined using flow cytometry. Changes in Bax and Bcl2 gene expression were investigated using real-time PCR. The MIC and the IC50 of the crude extract were calculated, and the MIC and IC50 of the Daphne extract in combination of imatinib were tested in the K-562 cell line. Results: K-562 cells responded to the extract treatments in a dose-dependent manner, and the increase in the expression of Bcl2 and decrease in the expression of the Bax gene intensified with increasing extract concentration. Flow cytometry revealed that most of the cells underwent necrosis. Conclusion: Daphne extract effectively decreased the viability of the K562 cell line. The necrotic effect of the Daphne extract was evaluated, and an increase in the gene expression of Bcl2 was observed in cells exposed to the Daphne extract. The combination of Daphne extracts with imatinib enhances the cytotoxic effect of imatinib.展开更多
Once nearly fatal, chronic myeloid leukemia (CML) has become a well-managed chronic disease for most patients thanks to tyrosine kinase inhibitors (TKIs). The improvement in treatment response with TKIs has introduced...Once nearly fatal, chronic myeloid leukemia (CML) has become a well-managed chronic disease for most patients thanks to tyrosine kinase inhibitors (TKIs). The improvement in treatment response with TKIs has introduced a new challenge in managing CML, as with most chronic diseases: therapeutic adherence. This study was conducted to evaluate the determinants of therapeutic adherence among patients. It was a descriptive and analytical cross-sectional study conducted from March 1, 2024, to June 1, 2024, in the hematology department of the CHU Campus. Data were collected using a questionnaire. Treatment adherence was assessed using the 8-item Morisky Medication Adherence Scale. The study included 63 patients. The mean age was 45.15 ± 15.95 years (range: 9 - 81 years), and the sex ratio was 1.73. Nineteen patients (30.17%) were highly adherent, 29 (46.03%) were moderately adherent, and 15 (23.80%) were poorly adherent. Factors increasing the likelihood of high adherence included older age, family support, and satisfaction with the information provided by the physician about CML. Patients who experienced side effects were more likely to be non-adherent compared to those who did not have side effects. Proximity to the hospital, educational and socioeconomic status, medication formulation, and dosage were not significantly associated with low adherence. Ultimately, our study revealed low therapeutic adherence among our patients, and this suggests two main areas for improvement to enhance treatment adherence: a focus on therapeutic education with clear and understandable information, and optimal management of side effects.展开更多
基金College of Agronomy and Biotechnology,Southwest University(4412200577).
文摘Calmodulin-like (CMLs) proteins are critical in calcium signaling and essential for plant growth, development, and stress responses. In many species, the CMLs families have been identified and described. However, the characterization and expression profiling of CMLs genes in tobacco is retrievable. In this study, a comprehensive whole-genome identification and analysis, and 75 NtCML genes were identified in tobacco, each containing two to four EF-hand domains. Most NtCML proteins exhibited conserved gene structures and motifs. Notably, most NtCML proteins were intron-less and distributed across 18 chromosomes. Two pairs of tandemly duplicated genes and seven pairs of segmentally duplicated genes were identified within the tobacco genome. Furthermore, 22 pairs of orthologous CMLs genes were discovered between Arabidopsis and tobacco. Cis-acting element analysis revealed that elements associated with hormones, stress responses, and plant growth and development were found in the promoter regions. Expression analysis indicated that some NtCML genes displayed tissue-specific expression patterns. Specifically, NtCML12, NtCML18, NtCML27, and NtCML28 showed significant upregulation during cold acclimation treatment. These results indicate that tobacco CMLs act as Ca^(2+) signal transducers, regulating plant growth and abiotic stress responses.
文摘【目的】鉴定青海茄参(Mandragora chinghaiensis Kuang et A.M.Jl)CML基因家族成员,探究其对冷胁迫的响应。【方法】基于课题组的全长转录组数据,利用生物信息学技术对青海茄参CML基因家族的成员及其蛋白理化性质、系统发育过程、基因结构等进行分析。【结果】在青海茄参中共鉴定出19个CML基因家族成员,亚细胞定位主要在细胞核,含有1~4个EF-hand结构域,二三级结构主要由α-螺旋组成;基序分析发现,McCMLs蛋白含有4个典型的EF手部基序;系统进化树分析显示,McCMLs蛋白被分为3个亚族。冷胁迫表达模式分析表明,McCML基因家族在冷胁迫下差异表达,其中McCML3、McCML7表达量显著上调。【结论】通过对McCML基因家族进行鉴定和分析,筛选出McCML3、McCML7作为青海茄参抗寒候选基因,为后期研究其抗寒分子机制提供理论基础。
基金grants from NSFC(31601728,31272142)Shandong Province(ZR2016CQ13,SDAIT-06-03)+1 种基金Young Scientists Funds of Shandong Agricultural University(564024)Youth Science and Technology Innovation Fund of Shandong Agricultural University(24024)
文摘Calcium(Ca^(2+)), a crucial second messenger in plants, is involved in diverse signaling pathways including biotic and abiotic stress responses. However, the functions of most calcium sensors including calcium-dependent protein kinases(CDPKs), calmodulins(Ca Ms), calmodulinlike proteins(CMLs), and calcineurin B-like proteins(CBLs) remain limited in plants, particularly in woody plants. Currently, a total of 83 CMLs and seven Ca Ms were discovered in the apple genome in this study. Functional domain analysis showed that these CMLs and Ca Ms contain a highly conserved EF-hand motif. q RT-PCR assays indicated that these CMLs were expressed ubiquitously in apple, including in the roots, stems,leaves, flowers, and fruits, and also possessed spatial specificity. Finally, most of these CMLs were induced by hormonal and abiotic stress, suggesting their potential roles in the regulation of growth, development, and the stress response in apple. In summary, our findings provide potential evidence that apple CMLs may be involved in abiotic stress and the regulation of plant growth and development.
文摘Background: Herbal medicine is well-known among the ancient medical sciences. Healing properties have been observed in some species of Daphne plant. The effect of Daphne plant extract on the K562 cell line has been previously studied, and Gleevec is a well-known and effective medicine for the treatment of chronic myelogenous leukemia. Material and Methods: In this study, the simultaneous effects of using herbal medicine and a target therapy medicine on the K562 cell line were investigated. The presence of some species of Daphne in Iran motivated us to evaluate the cytotoxic effect of Daphne mucronata on human leukemia cancer cells. The antiproliferative activity of the dichloromethane extract of Daphne mucronate (Thymelaeaceae), a new anticancer medicinal plant, was evaluated. Cell viability was quantitated by MTT assay. Apoptotic and necrotic changes in the cell membrane were examined using flow cytometry. Changes in Bax and Bcl2 gene expression were investigated using real-time PCR. The MIC and the IC50 of the crude extract were calculated, and the MIC and IC50 of the Daphne extract in combination of imatinib were tested in the K-562 cell line. Results: K-562 cells responded to the extract treatments in a dose-dependent manner, and the increase in the expression of Bcl2 and decrease in the expression of the Bax gene intensified with increasing extract concentration. Flow cytometry revealed that most of the cells underwent necrosis. Conclusion: Daphne extract effectively decreased the viability of the K562 cell line. The necrotic effect of the Daphne extract was evaluated, and an increase in the gene expression of Bcl2 was observed in cells exposed to the Daphne extract. The combination of Daphne extracts with imatinib enhances the cytotoxic effect of imatinib.
文摘Once nearly fatal, chronic myeloid leukemia (CML) has become a well-managed chronic disease for most patients thanks to tyrosine kinase inhibitors (TKIs). The improvement in treatment response with TKIs has introduced a new challenge in managing CML, as with most chronic diseases: therapeutic adherence. This study was conducted to evaluate the determinants of therapeutic adherence among patients. It was a descriptive and analytical cross-sectional study conducted from March 1, 2024, to June 1, 2024, in the hematology department of the CHU Campus. Data were collected using a questionnaire. Treatment adherence was assessed using the 8-item Morisky Medication Adherence Scale. The study included 63 patients. The mean age was 45.15 ± 15.95 years (range: 9 - 81 years), and the sex ratio was 1.73. Nineteen patients (30.17%) were highly adherent, 29 (46.03%) were moderately adherent, and 15 (23.80%) were poorly adherent. Factors increasing the likelihood of high adherence included older age, family support, and satisfaction with the information provided by the physician about CML. Patients who experienced side effects were more likely to be non-adherent compared to those who did not have side effects. Proximity to the hospital, educational and socioeconomic status, medication formulation, and dosage were not significantly associated with low adherence. Ultimately, our study revealed low therapeutic adherence among our patients, and this suggests two main areas for improvement to enhance treatment adherence: a focus on therapeutic education with clear and understandable information, and optimal management of side effects.
