Thermopsis turcica is an endemic species of wild legume in Türkiye. The plant produces flower pistils with unusual morphological properties. The aim of this study was to determine the factors controlling the inte...Thermopsis turcica is an endemic species of wild legume in Türkiye. The plant produces flower pistils with unusual morphological properties. The aim of this study was to determine the factors controlling the interesting feature of this plant. Particularly the controlling features of the flower meristem and the pistils that mutations in two genes and helps occurring the number of flower organs and can cause abnormalities, This study has attempted to explore the controlling feature of Thermopsis turcica, the young bud Arabidopsis CLV2, and CRC gene homologs were isolated and analyzed in different tissues. First, with total RNA isolated from the first thread degenerated primers synthesized cDNA expressions, second with a partial length cDNA cloning process made and the replicated set of analyses. The full-length cDNA of genes related to RACE analysis was carried out with the sequencing process. The real-time PCR method was applied for gene functional analyses with buds, sepals, petals and stamen and relative expression analysis of genes in the tissue of pistils. Full-length cDNA sequence for the gene CLV2 2557 bp, the encoded protein 716 amino acids, CRC 901 for full-length cDNA sequence of the gene—bp, the encoded protein 174 identified as amino acids. Alignment and phylogenetic analyses of CLV2 and CRC genes were found to be similar to other types of legumes in homologous sets. Real-time (RT-qPCR) PCR results relative to study courses with genes.展开更多
视盘,也称为电视唱片。粗浅地说,它是用唱片的形式记录图像信息和声音信息。当今世界上有三种视盘:CED视盘,它的特点是机械刻录,沟槽跟踪,电容针读取;VHD视盘,它的特点是激光刻录,无有沟槽,电容针读取;LVD视盘,它的特点是激光刻录,无有...视盘,也称为电视唱片。粗浅地说,它是用唱片的形式记录图像信息和声音信息。当今世界上有三种视盘:CED视盘,它的特点是机械刻录,沟槽跟踪,电容针读取;VHD视盘,它的特点是激光刻录,无有沟槽,电容针读取;LVD视盘,它的特点是激光刻录,无有沟槽,激光反射读取。 LVD视盘,就是激光视盘,它的英文全称是Laser Video Disc或Laser Vision(缩写为LV)。展开更多
Analysis and comparing with three existing and popularly used forcasting customer lifetime value (CLV) methods, which are the Dwyer method, customer event\|method and fitting method, and using performances of the exis...Analysis and comparing with three existing and popularly used forcasting customer lifetime value (CLV) methods, which are the Dwyer method, customer event\|method and fitting method, and using performances of the existent artificial neural network, we apply the Radial basis Function(RBF) network to forecast the CLV, the RBF network can approach the objective function partially. To every input/output pairs, it only needs adjust the weight a little and learn quickly which is very important to the forecast precision. Simulation and experimental results on the customers’ data of a company in Shaanxi Province reveal the effectiveness and feasibility of the RBF network.\;展开更多
Prolonged exposure to low temperatures during agricultural production often leads to fruit malformation in crops,significantly reducing market value.However,the underlying molecular mechanisms remain poorly understood...Prolonged exposure to low temperatures during agricultural production often leads to fruit malformation in crops,significantly reducing market value.However,the underlying molecular mechanisms remain poorly understood.In this study,we identify sugar transport protein 2(STP2)as a critical regulator of tomato fruit locule development under cold conditions.Low temperatures impair long-distance sucrose transport from leaves to shoot apices,resulting in reduced accumulation of glucose and arabinose.In response,STP2 expression is strongly upregulated in shoot apices,promoting glucose and arabinose transport.We found that the CLAVAT3-WUSCHEL(CLV3-WUS)regulatory module,which governs locule formation,relies on STP2-mediated sugar transport for CLV3 arabinosylation.Overexpression of STP2 promotes glucose and arabinose accumulation in shoot apices,enhances CLV3 arabinosylation and the WUS suppression,mitigating the multi-locular malformations induced by low temperatures.Conversely,disruption of STP2 function exacerbates locule number increases under low temperatures,which could not be rescued by exogenous sugar supplementation.Our findings reveal a key mechanism by which STP2-mediated sugar transport supports CLV3 arabinosylation to maintain fruit locule development under low temperatures,offering potential strategies to alleviate fruit malformations in winter crop cultivation.展开更多
文摘Thermopsis turcica is an endemic species of wild legume in Türkiye. The plant produces flower pistils with unusual morphological properties. The aim of this study was to determine the factors controlling the interesting feature of this plant. Particularly the controlling features of the flower meristem and the pistils that mutations in two genes and helps occurring the number of flower organs and can cause abnormalities, This study has attempted to explore the controlling feature of Thermopsis turcica, the young bud Arabidopsis CLV2, and CRC gene homologs were isolated and analyzed in different tissues. First, with total RNA isolated from the first thread degenerated primers synthesized cDNA expressions, second with a partial length cDNA cloning process made and the replicated set of analyses. The full-length cDNA of genes related to RACE analysis was carried out with the sequencing process. The real-time PCR method was applied for gene functional analyses with buds, sepals, petals and stamen and relative expression analysis of genes in the tissue of pistils. Full-length cDNA sequence for the gene CLV2 2557 bp, the encoded protein 716 amino acids, CRC 901 for full-length cDNA sequence of the gene—bp, the encoded protein 174 identified as amino acids. Alignment and phylogenetic analyses of CLV2 and CRC genes were found to be similar to other types of legumes in homologous sets. Real-time (RT-qPCR) PCR results relative to study courses with genes.
文摘视盘,也称为电视唱片。粗浅地说,它是用唱片的形式记录图像信息和声音信息。当今世界上有三种视盘:CED视盘,它的特点是机械刻录,沟槽跟踪,电容针读取;VHD视盘,它的特点是激光刻录,无有沟槽,电容针读取;LVD视盘,它的特点是激光刻录,无有沟槽,激光反射读取。 LVD视盘,就是激光视盘,它的英文全称是Laser Video Disc或Laser Vision(缩写为LV)。
文摘Analysis and comparing with three existing and popularly used forcasting customer lifetime value (CLV) methods, which are the Dwyer method, customer event\|method and fitting method, and using performances of the existent artificial neural network, we apply the Radial basis Function(RBF) network to forecast the CLV, the RBF network can approach the objective function partially. To every input/output pairs, it only needs adjust the weight a little and learn quickly which is very important to the forecast precision. Simulation and experimental results on the customers’ data of a company in Shaanxi Province reveal the effectiveness and feasibility of the RBF network.\;
基金supported by the Key Research and Development Program of China(2023YFD2000600)the National Natural Science Foundation of China(32430092)the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(SN-ZJU-SIAS-0011).
文摘Prolonged exposure to low temperatures during agricultural production often leads to fruit malformation in crops,significantly reducing market value.However,the underlying molecular mechanisms remain poorly understood.In this study,we identify sugar transport protein 2(STP2)as a critical regulator of tomato fruit locule development under cold conditions.Low temperatures impair long-distance sucrose transport from leaves to shoot apices,resulting in reduced accumulation of glucose and arabinose.In response,STP2 expression is strongly upregulated in shoot apices,promoting glucose and arabinose transport.We found that the CLAVAT3-WUSCHEL(CLV3-WUS)regulatory module,which governs locule formation,relies on STP2-mediated sugar transport for CLV3 arabinosylation.Overexpression of STP2 promotes glucose and arabinose accumulation in shoot apices,enhances CLV3 arabinosylation and the WUS suppression,mitigating the multi-locular malformations induced by low temperatures.Conversely,disruption of STP2 function exacerbates locule number increases under low temperatures,which could not be rescued by exogenous sugar supplementation.Our findings reveal a key mechanism by which STP2-mediated sugar transport supports CLV3 arabinosylation to maintain fruit locule development under low temperatures,offering potential strategies to alleviate fruit malformations in winter crop cultivation.
