Phylogenetic analysis and relative-rate test of 9 Clupeidae fishes were performed using the cytochrome b(cytb)gene sequence data.Based on Kimura-2-Parameter(K2P)distance,bootstrapped NJ tree(1000 replicates)was genera...Phylogenetic analysis and relative-rate test of 9 Clupeidae fishes were performed using the cytochrome b(cytb)gene sequence data.Based on Kimura-2-Parameter(K2P)distance,bootstrapped NJ tree(1000 replicates)was generated from these 9 cytb gene sequences of Clupeidae fishes.Three species of genus Sardinops are monophyletic group,2 species of genus Clupea are monophyletic group,and their bootstrap values are both 100%.Sardinella aurita and Sardinella maderensis are monophyletic group,their bootstrap value reached or exceed 50%.Spratttts spratttts which is a species of genus Sprattus,has a monophyletic origin with genus Clupea,their bootstrap value was 100%.The number of synonymous nucleotide substitution per synonymous site(dS)and the number of nonsynonymous nucleotide substitution per nonsynonymous site(dN)of cytb gene among Clupeidae fishes were calculated.The ratios of dN/dS were significantly less than l by Z-test,which suggest that purifying seiection may operate on the cytb gene for strong functional constraints.Relative-rate test which was based on Tajima's 1D and 2D tests suggested that molecular clock held within Clupeidae fishes,and the divergent time ranged from 0.12 to 13.45 million years within Clupeidae fishes.展开更多
文摘Phylogenetic analysis and relative-rate test of 9 Clupeidae fishes were performed using the cytochrome b(cytb)gene sequence data.Based on Kimura-2-Parameter(K2P)distance,bootstrapped NJ tree(1000 replicates)was generated from these 9 cytb gene sequences of Clupeidae fishes.Three species of genus Sardinops are monophyletic group,2 species of genus Clupea are monophyletic group,and their bootstrap values are both 100%.Sardinella aurita and Sardinella maderensis are monophyletic group,their bootstrap value reached or exceed 50%.Spratttts spratttts which is a species of genus Sprattus,has a monophyletic origin with genus Clupea,their bootstrap value was 100%.The number of synonymous nucleotide substitution per synonymous site(dS)and the number of nonsynonymous nucleotide substitution per nonsynonymous site(dN)of cytb gene among Clupeidae fishes were calculated.The ratios of dN/dS were significantly less than l by Z-test,which suggest that purifying seiection may operate on the cytb gene for strong functional constraints.Relative-rate test which was based on Tajima's 1D and 2D tests suggested that molecular clock held within Clupeidae fishes,and the divergent time ranged from 0.12 to 13.45 million years within Clupeidae fishes.
