Blood samples have traditionally been used as the main source of DNA for genetic analysis. How-ever, this source can be difficult in terms of collection, transportation, and long-term storage. In this study, we invest...Blood samples have traditionally been used as the main source of DNA for genetic analysis. How-ever, this source can be difficult in terms of collection, transportation, and long-term storage. In this study, we investigated whether human nail clippings could be used as a source of DNA for SNP genotyping, null-allele detection, and whole-genome amplification. From extracted nail DNA, we achieved amplicons up to a length of ~400 bp and >96% concordance for SNP genotyping and 100% concordance for null-allele detection compared to DNA derived from matched blood sam-ples. For whole-genome amplification, OmniPlex performed better than Multiple Displacement Amplification with a success rate of 89.3% and 76.8% for SNP genotyping and null-allele detection, respectively. Concordance was ~98% for both methods. When combined with OmniPlex whole-genome amplification, human nail clippings could potentially be used as an alternative to whole blood as a less invasive and more convenient source of DNA for genotyping studies.展开更多
Introduction Extensive Reading, as the name indicates, is a guide for students into the vast sea of knowledge. Reading demonstrates to students that there is no end in learning knowledge. However, reading and language...Introduction Extensive Reading, as the name indicates, is a guide for students into the vast sea of knowledge. Reading demonstrates to students that there is no end in learning knowledge. However, reading and language learning based on textbooks is far from enough because the knowledge from them is too narrow, the vocabulary is too limited and the contents are not current enough in these times of information exchange. Throughout my teaching experiments, I have found that newspaper clippings can become a very good way to bring more benefits to students’ learning than I expected. It can broaden the students’ horizons,展开更多
文摘Blood samples have traditionally been used as the main source of DNA for genetic analysis. How-ever, this source can be difficult in terms of collection, transportation, and long-term storage. In this study, we investigated whether human nail clippings could be used as a source of DNA for SNP genotyping, null-allele detection, and whole-genome amplification. From extracted nail DNA, we achieved amplicons up to a length of ~400 bp and >96% concordance for SNP genotyping and 100% concordance for null-allele detection compared to DNA derived from matched blood sam-ples. For whole-genome amplification, OmniPlex performed better than Multiple Displacement Amplification with a success rate of 89.3% and 76.8% for SNP genotyping and null-allele detection, respectively. Concordance was ~98% for both methods. When combined with OmniPlex whole-genome amplification, human nail clippings could potentially be used as an alternative to whole blood as a less invasive and more convenient source of DNA for genotyping studies.
文摘Introduction Extensive Reading, as the name indicates, is a guide for students into the vast sea of knowledge. Reading demonstrates to students that there is no end in learning knowledge. However, reading and language learning based on textbooks is far from enough because the knowledge from them is too narrow, the vocabulary is too limited and the contents are not current enough in these times of information exchange. Throughout my teaching experiments, I have found that newspaper clippings can become a very good way to bring more benefits to students’ learning than I expected. It can broaden the students’ horizons,
