Arthropod melanization is a crucial defense mechanism mediated by a complex cascade of CLIP domain serine proteases(CLIPs).In this study,it was confirmed that microRNA-11903a(miR-11903a)targets Aedes-CLIPB9(AeCLIPB9)b...Arthropod melanization is a crucial defense mechanism mediated by a complex cascade of CLIP domain serine proteases(CLIPs).In this study,it was confirmed that microRNA-11903a(miR-11903a)targets Aedes-CLIPB9(AeCLIPB9)by bioinformatics prediction and dual-luciferase reporter assays.Following intrathoracic injection of miR-11903a agomir and antagomir,Real-time quantitative polymerase chain reaction confirmed that AeCLIPB9 is negatively regulated by miR-11903a.Spatiotemporal expression analysis revealed that miR-11903a is most abundant in 4th instar larvae,followed by pupae and adults,and highly expressed in the wings,head,and midgut of female adults.Following pathogen infection,AeCLIPB9 and miR-11903a exhibited opposite expression trends,indicating their potential roles in mosquito innate immunity.To further investigate the relationship between AeCLIPB9 and miR-11903a,double-strand CLIPB9 was synthesized and RNA interference was performed.Seven-d survival assays revealed that both AeCLIPB9 and miR-11903a were crucial immune factors in fighting pathogens.Finally,longevity assays demonstrated that miR-11903a influenced mosquito lifespan.展开更多
BACKGROUND Secure transluminal closure remains a fundamental barrier to endoscopic surgery.It has been reported that through-the-scope clips were used to secure the incision of the gallbladder during natural orifice t...BACKGROUND Secure transluminal closure remains a fundamental barrier to endoscopic surgery.It has been reported that through-the-scope clips were used to secure the incision of the gallbladder during natural orifice transluminal endoscopic cholecystolithotomy and were left in the body post-operation.The over-the-scope clip(OTSC)is favored for its rapid deployment and strong anchoring capabilities.Nevertheless,OTSCs are difficult to remove once implanted.The Senscure Biotechnology in China has developed a detachable over-the-scope clip(D-OTSC)for this purpose.Here,we utilized the D-OTSC to successfully close a full-thickness sigmoid defect exceeding 1 cm in diameter.Subsequently,the clip was completely removed postoperatively,yielding favorable clinical outcomes.CASE SUMMARY We present the case of a 51-year-old female patient who underwent natural orifice transluminal endoscopic cholecystolithotomy.The sigmoid incision was closed using a D-OTSC.Postoperative recovery was uneventful,with no abdominal infection or bleeding.The D-OTSC was subsequently removed via enteroscopy in the outpatient department one month later.CONCLUSION The utilization of D-OTSC presents a viable option for closing colonic mucosal incisions ranging from 1 cm to 2 cm.展开更多
当前,全球科技创新呈现高速发展和高度融合的态势。准确识别出颠覆性技术主题以推动全面创新已成为科学技术发展和经济增长的关键动力。然而,传统的颠覆性技术主题识别方法主要依赖于单一模态数据,存在一定的局限性。本文基于CLIP(contr...当前,全球科技创新呈现高速发展和高度融合的态势。准确识别出颠覆性技术主题以推动全面创新已成为科学技术发展和经济增长的关键动力。然而,传统的颠覆性技术主题识别方法主要依赖于单一模态数据,存在一定的局限性。本文基于CLIP(contrastive language-image pre-training)和LDAGV(linear discriminant analysis&global vectors for word representation)模型构建新闻文本与图像特征融合向量,通过k-means聚类迭代并结合3个颠覆性技术主题指标进行筛选,实现了多模态信息的融合以及主题的精准识别。以新能源领域为例,验证了该模型在颠覆性技术主题识别方面的可行性和有效性。与其他单一模态模型相比,多模态信息融合模型在颠覆性技术主题识别方面更具优势。展开更多
基金supported by the Major Science and Technology Plan of Hainan Province(ZDKJ2021035)the National Natural Science Foundation of China(U22A20363)the Innovative Research Projects for Postgraduates in Hainan Province(Qhyb2022-41).
文摘Arthropod melanization is a crucial defense mechanism mediated by a complex cascade of CLIP domain serine proteases(CLIPs).In this study,it was confirmed that microRNA-11903a(miR-11903a)targets Aedes-CLIPB9(AeCLIPB9)by bioinformatics prediction and dual-luciferase reporter assays.Following intrathoracic injection of miR-11903a agomir and antagomir,Real-time quantitative polymerase chain reaction confirmed that AeCLIPB9 is negatively regulated by miR-11903a.Spatiotemporal expression analysis revealed that miR-11903a is most abundant in 4th instar larvae,followed by pupae and adults,and highly expressed in the wings,head,and midgut of female adults.Following pathogen infection,AeCLIPB9 and miR-11903a exhibited opposite expression trends,indicating their potential roles in mosquito innate immunity.To further investigate the relationship between AeCLIPB9 and miR-11903a,double-strand CLIPB9 was synthesized and RNA interference was performed.Seven-d survival assays revealed that both AeCLIPB9 and miR-11903a were crucial immune factors in fighting pathogens.Finally,longevity assays demonstrated that miR-11903a influenced mosquito lifespan.
基金Supported by Natural Science Foundation of Fujian Province,China,No.2021J01545.
文摘BACKGROUND Secure transluminal closure remains a fundamental barrier to endoscopic surgery.It has been reported that through-the-scope clips were used to secure the incision of the gallbladder during natural orifice transluminal endoscopic cholecystolithotomy and were left in the body post-operation.The over-the-scope clip(OTSC)is favored for its rapid deployment and strong anchoring capabilities.Nevertheless,OTSCs are difficult to remove once implanted.The Senscure Biotechnology in China has developed a detachable over-the-scope clip(D-OTSC)for this purpose.Here,we utilized the D-OTSC to successfully close a full-thickness sigmoid defect exceeding 1 cm in diameter.Subsequently,the clip was completely removed postoperatively,yielding favorable clinical outcomes.CASE SUMMARY We present the case of a 51-year-old female patient who underwent natural orifice transluminal endoscopic cholecystolithotomy.The sigmoid incision was closed using a D-OTSC.Postoperative recovery was uneventful,with no abdominal infection or bleeding.The D-OTSC was subsequently removed via enteroscopy in the outpatient department one month later.CONCLUSION The utilization of D-OTSC presents a viable option for closing colonic mucosal incisions ranging from 1 cm to 2 cm.
文摘当前,全球科技创新呈现高速发展和高度融合的态势。准确识别出颠覆性技术主题以推动全面创新已成为科学技术发展和经济增长的关键动力。然而,传统的颠覆性技术主题识别方法主要依赖于单一模态数据,存在一定的局限性。本文基于CLIP(contrastive language-image pre-training)和LDAGV(linear discriminant analysis&global vectors for word representation)模型构建新闻文本与图像特征融合向量,通过k-means聚类迭代并结合3个颠覆性技术主题指标进行筛选,实现了多模态信息的融合以及主题的精准识别。以新能源领域为例,验证了该模型在颠覆性技术主题识别方面的可行性和有效性。与其他单一模态模型相比,多模态信息融合模型在颠覆性技术主题识别方面更具优势。
