Objective To evaluate the application of weak cation exchange (WCX) magnetic bead-based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in detecting differentially expressed...Objective To evaluate the application of weak cation exchange (WCX) magnetic bead-based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in detecting differentially expressed proteins in the urine of renal clear cell carcinoma (RCCC) and its value in the early diagnosis of RCCC.Methods Eleven newly diagnosed patients (10 males and 1 female, aged 46-78, mean 63 years) of renal clear cell carcinoma by biopsy and 10 healthy volunteers (all males, aged 25-32, mean 29.7 years) were enrolled in this study. Urine samples of the RCCC patients and healthy controls were collected in the morning.Weak cation exchange (WCX) bead-based MALDI-TOF MS technique was applied in detecting differential protein peaks in the urine of RCCC. ClinProTools2.2 software was utilized to determine the characteristic proteins in the urine of RCCC patients for the predictive model of RCCC.Results The technique identified 160 protein peaks in the urine that were different between RCCC patients and health controls; and among them, there was one peak (molecular weight of 2221.71 Da) with statistical significance (P=0.0304). With genetic algorithms and the support vector machine, we screened out 13 characteristic protein peaks for the predictive model.Conclusions The application of WCX magnetic bead-based MALDI-TOF MS in detecting differentiallyexpressed proteins in urine may have potential value for the early diagnosis of RCCC.展开更多
目的 评估全面质量管理(overall every control and clear,OEC)模式应用在医用耗材管理中的效果。方法 选取2023年7月—2024年6月天门市第一人民医院介入科的300份医用耗材管理数据进行研究。将2023年7—12月采用常规医用耗材管理模式的...目的 评估全面质量管理(overall every control and clear,OEC)模式应用在医用耗材管理中的效果。方法 选取2023年7月—2024年6月天门市第一人民医院介入科的300份医用耗材管理数据进行研究。将2023年7—12月采用常规医用耗材管理模式的150份医用耗材管理数据纳入对照组,将2024年1—6月采用OEC模式的150份医用耗材管理数据纳入观察组,2组管理人员相同。比较2组耗材管理时间、一次性计费正确率、出库完成率、不良事件发生率、医护人员满意度。结果 观察组医用耗材盘点时间、订单响应时间、验收上架时间、采购时间分别为(23.57±3.15)min、(5.09±1.03)h、(19.36±1.73)min、(14.32±1.60)min,均短于对照组的(39.33±6.89)min、(10.36±1.34)h、(33.33±6.68)min、(34.66±1.87)min,差异有统计学意义(P<0.001)。观察组医用耗材一次性计费正确率、出库完成率分别为98.00%、98.67%,均高于对照组的86.00%、86.67%,差异有统计学意义(P<0.001)。观察组医用耗材不良事件总发生率为1.33%,低于对照组8.00%,差异有统计学意义(P<0.05)。观察组医护人员对耗材管理的总满意度为100%,高于对照组的80.00%,差异有统计学意义(P<0.05)。结论 在医用耗材管理过程中采用OEC模式可以缩短耗材管理时间,提高耗材管理质量及效率,减少不良事件,医护人员满意度高。展开更多
文摘Objective To evaluate the application of weak cation exchange (WCX) magnetic bead-based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in detecting differentially expressed proteins in the urine of renal clear cell carcinoma (RCCC) and its value in the early diagnosis of RCCC.Methods Eleven newly diagnosed patients (10 males and 1 female, aged 46-78, mean 63 years) of renal clear cell carcinoma by biopsy and 10 healthy volunteers (all males, aged 25-32, mean 29.7 years) were enrolled in this study. Urine samples of the RCCC patients and healthy controls were collected in the morning.Weak cation exchange (WCX) bead-based MALDI-TOF MS technique was applied in detecting differential protein peaks in the urine of RCCC. ClinProTools2.2 software was utilized to determine the characteristic proteins in the urine of RCCC patients for the predictive model of RCCC.Results The technique identified 160 protein peaks in the urine that were different between RCCC patients and health controls; and among them, there was one peak (molecular weight of 2221.71 Da) with statistical significance (P=0.0304). With genetic algorithms and the support vector machine, we screened out 13 characteristic protein peaks for the predictive model.Conclusions The application of WCX magnetic bead-based MALDI-TOF MS in detecting differentiallyexpressed proteins in urine may have potential value for the early diagnosis of RCCC.
文摘目的 评估全面质量管理(overall every control and clear,OEC)模式应用在医用耗材管理中的效果。方法 选取2023年7月—2024年6月天门市第一人民医院介入科的300份医用耗材管理数据进行研究。将2023年7—12月采用常规医用耗材管理模式的150份医用耗材管理数据纳入对照组,将2024年1—6月采用OEC模式的150份医用耗材管理数据纳入观察组,2组管理人员相同。比较2组耗材管理时间、一次性计费正确率、出库完成率、不良事件发生率、医护人员满意度。结果 观察组医用耗材盘点时间、订单响应时间、验收上架时间、采购时间分别为(23.57±3.15)min、(5.09±1.03)h、(19.36±1.73)min、(14.32±1.60)min,均短于对照组的(39.33±6.89)min、(10.36±1.34)h、(33.33±6.68)min、(34.66±1.87)min,差异有统计学意义(P<0.001)。观察组医用耗材一次性计费正确率、出库完成率分别为98.00%、98.67%,均高于对照组的86.00%、86.67%,差异有统计学意义(P<0.001)。观察组医用耗材不良事件总发生率为1.33%,低于对照组8.00%,差异有统计学意义(P<0.05)。观察组医护人员对耗材管理的总满意度为100%,高于对照组的80.00%,差异有统计学意义(P<0.05)。结论 在医用耗材管理过程中采用OEC模式可以缩短耗材管理时间,提高耗材管理质量及效率,减少不良事件,医护人员满意度高。
