A new isochromanone,cladosporinisochromanone(1),accompanied by 15 known compounds(2–16)were obtained from secondary metabolites produced by marine-derived fungus Cladosporium sp.DLT-5.NMR and HRESIMS spectra elucidat...A new isochromanone,cladosporinisochromanone(1),accompanied by 15 known compounds(2–16)were obtained from secondary metabolites produced by marine-derived fungus Cladosporium sp.DLT-5.NMR and HRESIMS spectra elucidation determined the planar structure of 1.Subsequent electronic circular dichroism(ECD)experiment assigned the absolute configuration of 1.Compounds 1,2,4–6,and 10 displayed different degrees of neuroprotective activities on human neuroblastoma cells SH-SY5Y.Five compounds(1,3–5,and 13)emerged resistance to protein tyrosine phosphatase 1B(PTP1B),further kinetic analysis and molecular docking study indicated that the most potent compound 13(IC50value of 10.74±0.61μmol/L)was found as a noncompetitive inhibitor for PTP1B.Surface plasmon resonance(SPR)and molecular docking studies also demonstrated the interaction between compound 12 and Niemann-Pick C1 Like 1(NPC1L1),which has been identified as significant therapeutic target for hypercholesteremia.In addition,compounds 3,6,and 14 showed attractive inhibitory activity against the phytopathogenic fungi:Colletotrichum capsici.Therefore,library of Cladosporium metabolites is enriched and new active uses of known compounds are explored.展开更多
A new nine-membered lactone,cladospolide E（1）,was isolated from a culture broth of a marine fungus Cladosporium sp.F14. The structure of compound 1 was determined on the basis of extensive spectroscopic analysis,inc...A new nine-membered lactone,cladospolide E（1）,was isolated from a culture broth of a marine fungus Cladosporium sp.F14. The structure of compound 1 was determined on the basis of extensive spectroscopic analysis,including 1D and 2D NMR data.展开更多
Cladosporium cladosporioides is an emerging pathogenic fungus that causes Cladosporium rot in postharvest table grapes(Vitis vinifera). However,studies investigating the infection process of C. cladosporioides are lac...Cladosporium cladosporioides is an emerging pathogenic fungus that causes Cladosporium rot in postharvest table grapes(Vitis vinifera). However,studies investigating the infection process of C. cladosporioides are lacking. Therefore, this study aimed to elucidate the infection process by investigating the influence of microclimatic parameters(temperature, wetness and fungal age) in C. cladosporioides pathogenesis, activities of grape defense-related enzymes and grape quality during the infection. C. cladosporioides effectively infects grapes by developing distinct colonies on the artificial wounds and berry surfaces, completing its life cycle within 48 h. The C. cladosporioides disease incidence optimally occurred at 20 ℃ and 25 ℃. Wetness played an influential role in the infectivity of C. cladosporioides and 7-day-old C. cladosporioides resulted in the most serious disease incidence of table grapes. As a result of infection, the quality of grapes was affected, including berry weight, p H, titratable acidity(TA), total soluble solids(TSS), and ascorbic acid level. This infection also induced defense-related enzymes, including polyphenoloxidase(PPO), peroxidase(POD), phenylalanine ammonialyase(PAL), and catalase(CAT), at certain times. The findings of this study demonstrated that Cladosporium rot development depended on the microclimatic parameters of grapes, significantly affected the grape quality and induced grape's defense-related enzymes.展开更多
Three main phytotoxic compounds including lunatoic acid A （1）, 5Z-7-oxozeaenol （2） and zeaenol （3） were isolated from the fermentation broth of Cladosporium oxysporum DH14, a fungus residing in the locust （Oxya...Three main phytotoxic compounds including lunatoic acid A （1）, 5Z-7-oxozeaenol （2） and zeaenol （3） were isolated from the fermentation broth of Cladosporium oxysporum DH14, a fungus residing in the locust （Oxya chinensis ） gut. Two additional derivative compounds, compound la and lb, were synthesized by methylation and chlorination of compound 1, respectively. The structures of such compounds were identified on the basis of spectroscopic analysis and by comparison of the corresponding data to those previously reported in the literature. Compounds 1-3 exhibited significantly phytotoxic activities against the radicle growth of Amaranthus retroflexus L. with the 50% inhibitory concentrations （ICso values） of 4.51, 4.80 and 8.16 μg· mL^-1, respectively, which is comparable to that positive control 2,4-dichlorophenoxyacetic acid （IC50=1.95 μg· mL^-1）. Furthermore, the compound 1 showed selective phytotoxic activity with the inhibition rate of less than 22% against the crops of Brassica rapa L., Sorghum durra, Brassica campestris L,, Capsicum annucm and Raphanus safivus L. under the concentration of 100 μg· mL^-1 Both derivatives of compound 1 had moderate phytotoxic activity against the radicle growth of A. retroflexus L. The findings of our present study suggest that these compounds provide new promising candidates for the potential management strategies of weeds.展开更多
BACKGROUND Cladosporium and Corynespora cassiicola(C.cassiicola)infections rarely occur in humans.Mutations in human caspase recruitment domain protein 9(CARD9)are reported to be associated with fungal diseases.Pulmon...BACKGROUND Cladosporium and Corynespora cassiicola(C.cassiicola)infections rarely occur in humans.Mutations in human caspase recruitment domain protein 9(CARD9)are reported to be associated with fungal diseases.Pulmonary Cladosporium infection coexisting with subcutaneous C.cassiicola infection in a patient with a CARD9 mutation has not been reported in the literature.CASE SUMMARY A 68-year-old male patient was hospitalized for hypertrophic erythema and deep ulcers on the left upper extremity.He was diagnosed with pneumonia caused by Cladosporium,as identified through bronchoalveolar lavage fluid analysis,and deep dermatophytosis caused by C.cassiicola,as identified through morphological characteristics of the wound secretion culture.He underwent antifungal therapy(voriconazole)and recovered successfully.He carried two mutations in CARD9(chr9:139266425 and chr9:139262240)and was therefore susceptible to fungal infections.CONCLUSION This case study is the first to report the coexistence of pulmonary Cladosporium infection and subcutaneous C.cassiicola infection in a patient with CARD9 mutation.Our findings will be helpful in enriching the phenotypic spectrum of fungal infections underlying CARD9 deficiency.展开更多
基金Supported by the China Agriculture Research System of MOF and MARA(CARS-21)the Financial Fund of the Ministry of Agriculture and Rural Affairs,China(No.NFZX2021)the National Natural Science Foundation of China(No.81973568)。
文摘A new isochromanone,cladosporinisochromanone(1),accompanied by 15 known compounds(2–16)were obtained from secondary metabolites produced by marine-derived fungus Cladosporium sp.DLT-5.NMR and HRESIMS spectra elucidation determined the planar structure of 1.Subsequent electronic circular dichroism(ECD)experiment assigned the absolute configuration of 1.Compounds 1,2,4–6,and 10 displayed different degrees of neuroprotective activities on human neuroblastoma cells SH-SY5Y.Five compounds(1,3–5,and 13)emerged resistance to protein tyrosine phosphatase 1B(PTP1B),further kinetic analysis and molecular docking study indicated that the most potent compound 13(IC50value of 10.74±0.61μmol/L)was found as a noncompetitive inhibitor for PTP1B.Surface plasmon resonance(SPR)and molecular docking studies also demonstrated the interaction between compound 12 and Niemann-Pick C1 Like 1(NPC1L1),which has been identified as significant therapeutic target for hypercholesteremia.In addition,compounds 3,6,and 14 showed attractive inhibitory activity against the phytopathogenic fungi:Colletotrichum capsici.Therefore,library of Cladosporium metabolites is enriched and new active uses of known compounds are explored.
基金The authors are grateful to the Knowledge Innovation Program of Chinese Academy of Science(Nos.KZCX2-YW-216-1 and KSCX2-YW-G-073)the National Basic Research Program of China(No.2010CB833800-G)+1 种基金the National Natural Science Foundation of China and Research Grants Council of Hong Kong(NSFC/RGC) Program(No. 40910093)Knowledge Innovation Program of South China Sea Institute of Oceanology,Chinese Academy of Sciences(No.LYQY200703) for financial support
文摘A new nine-membered lactone,cladospolide E（1）,was isolated from a culture broth of a marine fungus Cladosporium sp.F14. The structure of compound 1 was determined on the basis of extensive spectroscopic analysis,including 1D and 2D NMR data.
基金the financial support received from National Natural Science Foundation of China (Grant Nos. 31950410556, 31901743, and 31971795)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (Grant No. PAPD-2018-87)。
文摘Cladosporium cladosporioides is an emerging pathogenic fungus that causes Cladosporium rot in postharvest table grapes(Vitis vinifera). However,studies investigating the infection process of C. cladosporioides are lacking. Therefore, this study aimed to elucidate the infection process by investigating the influence of microclimatic parameters(temperature, wetness and fungal age) in C. cladosporioides pathogenesis, activities of grape defense-related enzymes and grape quality during the infection. C. cladosporioides effectively infects grapes by developing distinct colonies on the artificial wounds and berry surfaces, completing its life cycle within 48 h. The C. cladosporioides disease incidence optimally occurred at 20 ℃ and 25 ℃. Wetness played an influential role in the infectivity of C. cladosporioides and 7-day-old C. cladosporioides resulted in the most serious disease incidence of table grapes. As a result of infection, the quality of grapes was affected, including berry weight, p H, titratable acidity(TA), total soluble solids(TSS), and ascorbic acid level. This infection also induced defense-related enzymes, including polyphenoloxidase(PPO), peroxidase(POD), phenylalanine ammonialyase(PAL), and catalase(CAT), at certain times. The findings of this study demonstrated that Cladosporium rot development depended on the microclimatic parameters of grapes, significantly affected the grape quality and induced grape's defense-related enzymes.
基金the National Natural Science Foundation of China (NSFC) (21002092 and 21272215)the Open Project of State Key Laboratory of Pharmaceutical Biotechnology in Nanjing University, China (KF-GN-201411)
文摘Three main phytotoxic compounds including lunatoic acid A （1）, 5Z-7-oxozeaenol （2） and zeaenol （3） were isolated from the fermentation broth of Cladosporium oxysporum DH14, a fungus residing in the locust （Oxya chinensis ） gut. Two additional derivative compounds, compound la and lb, were synthesized by methylation and chlorination of compound 1, respectively. The structures of such compounds were identified on the basis of spectroscopic analysis and by comparison of the corresponding data to those previously reported in the literature. Compounds 1-3 exhibited significantly phytotoxic activities against the radicle growth of Amaranthus retroflexus L. with the 50% inhibitory concentrations （ICso values） of 4.51, 4.80 and 8.16 μg· mL^-1, respectively, which is comparable to that positive control 2,4-dichlorophenoxyacetic acid （IC50=1.95 μg· mL^-1）. Furthermore, the compound 1 showed selective phytotoxic activity with the inhibition rate of less than 22% against the crops of Brassica rapa L., Sorghum durra, Brassica campestris L,, Capsicum annucm and Raphanus safivus L. under the concentration of 100 μg· mL^-1 Both derivatives of compound 1 had moderate phytotoxic activity against the radicle growth of A. retroflexus L. The findings of our present study suggest that these compounds provide new promising candidates for the potential management strategies of weeds.
文摘BACKGROUND Cladosporium and Corynespora cassiicola(C.cassiicola)infections rarely occur in humans.Mutations in human caspase recruitment domain protein 9(CARD9)are reported to be associated with fungal diseases.Pulmonary Cladosporium infection coexisting with subcutaneous C.cassiicola infection in a patient with a CARD9 mutation has not been reported in the literature.CASE SUMMARY A 68-year-old male patient was hospitalized for hypertrophic erythema and deep ulcers on the left upper extremity.He was diagnosed with pneumonia caused by Cladosporium,as identified through bronchoalveolar lavage fluid analysis,and deep dermatophytosis caused by C.cassiicola,as identified through morphological characteristics of the wound secretion culture.He underwent antifungal therapy(voriconazole)and recovered successfully.He carried two mutations in CARD9(chr9:139266425 and chr9:139262240)and was therefore susceptible to fungal infections.CONCLUSION This case study is the first to report the coexistence of pulmonary Cladosporium infection and subcutaneous C.cassiicola infection in a patient with CARD9 mutation.Our findings will be helpful in enriching the phenotypic spectrum of fungal infections underlying CARD9 deficiency.
