Aiming to meet the demand of the country＇ s nuclear demonstration project on the CAP1400 nuclear power plant, Baosteel uses the roll-bonding technology and develops the SA-533 Type B CL. 1 ＋ SA-240 Type 304L high-st...Aiming to meet the demand of the country＇ s nuclear demonstration project on the CAP1400 nuclear power plant, Baosteel uses the roll-bonding technology and develops the SA-533 Type B CL. 1 ＋ SA-240 Type 304L high-strength and high-toughness clad steel plate with a shear strength of over 310 MPa for the nuclear power plant＇ s safety injection tank. The properties of the quenched and tempered and the simulated post-weld heat treatment states are systematically studied herein through a comprehensive inspection and evaluation of the composition,microstructure,and properties of the clad steel plate. The results show that the bonding interface has high shear strength and that the base metal has high strength and good toughness at low temperatures. Hence, the performance fully meets the technical requirements of the CAP1400 nuclear power plant＇ s safety injection tank in the country＇ s nuclear demonstration project. The roll-bonded clad steel plate can be used to manufacture the safety injection tank of the CAP1400 nuclear power plant.展开更多
文摘目的探讨琥珀酸脱氢酶复合物亚单位A(SDHA)对小鼠肝细胞BNL CL.2细胞增殖、细胞周期和凋亡的影响。方法用慢病毒载体介导的sh RNA敲低BNL CL.2细胞中sdha基因的表达。流式细胞仪检测慢病毒的感染效率;实时荧光定量PCR和Western蛋白印迹法分别检测sdha m RNA和SDHA蛋白表达水平;细胞计数法检测细胞增殖;流式细胞术检测细胞凋亡和细胞周期。结果无相关sh RNA对照组和sdha sh RNA组慢病毒的感染效率均>80%。与无相关sh RNA对照组相比,感染sdha sh RNA慢病毒载体的BNL CL.2细胞sdha m RNA水平降低20倍左右(P<0.01),蛋白表达水平降低10倍左右(P<0.01);细胞增殖速度减慢,为无相关sh RNA对照组的70%左右(P<0.05);细胞周期发生改变,G0/G1期细胞百分率是无相关sh RNA对照组的1.17倍(P<0.01),G2/M期细胞百分率为1.37倍(P<0.01),S期细胞百分率为73.8%(P<0.01);但细胞凋亡率无明显差异。结论降低SDHA表达对小鼠肝细胞BNL CL.2细胞增殖有明显的抑制作用,其抑制作用与细胞周期阻滞相关,与细胞凋亡无明显关系。
文摘Aiming to meet the demand of the country＇ s nuclear demonstration project on the CAP1400 nuclear power plant, Baosteel uses the roll-bonding technology and develops the SA-533 Type B CL. 1 ＋ SA-240 Type 304L high-strength and high-toughness clad steel plate with a shear strength of over 310 MPa for the nuclear power plant＇ s safety injection tank. The properties of the quenched and tempered and the simulated post-weld heat treatment states are systematically studied herein through a comprehensive inspection and evaluation of the composition,microstructure,and properties of the clad steel plate. The results show that the bonding interface has high shear strength and that the base metal has high strength and good toughness at low temperatures. Hence, the performance fully meets the technical requirements of the CAP1400 nuclear power plant＇ s safety injection tank in the country＇ s nuclear demonstration project. The roll-bonded clad steel plate can be used to manufacture the safety injection tank of the CAP1400 nuclear power plant.
