Background:“Qi deficiency”(a pathological state where the body’s vital energy(Qi)is insufficient or weakened,impairing physiological functions and diminishing the body’s ability to perform daily activities,defend ...Background:“Qi deficiency”(a pathological state where the body’s vital energy(Qi)is insufficient or weakened,impairing physiological functions and diminishing the body’s ability to perform daily activities,defend against illness,and maintain homeostasis)syndrome is considered a critical syndrome in traditional Chinese medicine(TCM)and is associated with poor prognosis in heart failure(HF).This study investigates the clinical,metabolic,and transcriptomic differences between heart failure patients with and without Qi deficiency syndrome.Methods:56 heart failure patients were evaluated using a Qi deficiency syndrome scale and divided into Qi deficiency syndrome(QD)and non-Qi deficiency(non-QD)groups based on the median score.Clinical characteristics,including baseline N-terminal pro-B-type natriuretic peptide(NT-proBNP),left ventricular ejection fraction(LVEF),total diuretic use during hospitalization,and 90-day rehospitalization rates,were compared between the groups.Differentially expressed genes(DEGs)and differential metabolites were identified,followed by enrichment analyses and validation using qPCR and Western blot in AC16 cardiomyocytes.Results:QD patients exhibited significantly higher NT-proBNP levels,lower LVEF,and increased 90-day rehospitalization rates.Metabolomic profiling revealed lipid metabolism disruptions,notably in linoleic acid and phospholipid pathways.Transcriptomic analysis highlighted 17 DEGs,including CISD2,a critical mitochondrial regulator,which was downregulated in QD patients.Correlation analysis identified significant associations between DEGs(e.g.,CISD2,BPGM)and lipid metabolites such as PC(16:0/P-16:0).Functional knockdown of CISD2 in AC16 cells led to upregulation of lipid oxidation enzymes ALOX15 and CYP1A2,linking CISD2 dysfunction to lipid metabolic dysregulation.Conclusion:Qi deficiency is associated with more severe heart failure symptoms,worse prognosis,and distinct metabolic and transcriptomic profiles,particularly in lipid metabolism.CISD2 emerges as a potential therapeutic target,offering new avenues for integrating molecular insights with TCM approaches to optimize HF management.展开更多
Multiple protective effects of curcumin in cases of spinal cord injuries（SCIs）：Curcumin[1,7-bis（4-hydroxy-3-methoxyphenyl）-1,6-heptadiene-3,5-dione]is a nonsteroidal,naturally occurring compound commonly utilized...Multiple protective effects of curcumin in cases of spinal cord injuries（SCIs）：Curcumin[1,7-bis（4-hydroxy-3-methoxyphenyl）-1,6-heptadiene-3,5-dione]is a nonsteroidal,naturally occurring compound commonly utilized as a dietary pigment as well as a spice in India.It is obtained from curcuma longa in.展开更多
CISD1,an outer mitochondrial membrane iron-sulfur cluster protein,regulates intracellular iron levels,oxidative stress,and mitochondrial dynamics,playing critical roles in cellular bioenergetics and redox homeostasis....CISD1,an outer mitochondrial membrane iron-sulfur cluster protein,regulates intracellular iron levels,oxidative stress,and mitochondrial dynamics,playing critical roles in cellular bioenergetics and redox homeostasis.Although CISD1 has been identified as a prognostic biomarker in specific cancers,its broader implications in tumorigenesis,cancer progression,and immunotherapy remain unclear.Given the heterogeneity of cancer and the need for robust biomarkers across cancers,this study conducts the first comprehensive pan-cancer analysis of CISD1 by evaluating its roles in cancer and treatment.We obtained and analyzed data from databases including TCGA,GTEx,THPA,GEPIA2.0,SangerBox,cBioPortal,TIMER2.0,CAMOIP,DAVID,SRPLOT,and TISIDB.Our findings reveal significant alterations in CISD1 expression at both transcriptional and translational levels,as well as gene mutations across multiple cancers,indicating its potential as a diagnostic biomarker and its involvement in cancer development and progression.CISD1 dysregulation is linked to poor clinical outcomes,as shown through its impact on patient prognosis.GO and KEGG analyses show that CISD1 plays critical roles in cellular bioenergetics.Notably,CISD1 expression is significantly correlated with tumor stemness indices,tumor mutation burden,microsatellite instability,and immune checkpoint proteins in multiple cancers,and altered CISD1 levels are also observed in patients responding to immunotherapy,further supporting its role not only in prognosis but also as a key predictor in immunotherapy responses and outcomes.Our findings demonstrate CISD1 as a reliable and promising diagnostic,prognostic,and immunotherapeutic biomarker for multiple cancers,emphasizing its crucial role in cancer biology and potential to guide personalized cancer therapies.展开更多
A novel method which makes full use of point groups including Abeian and nonAbelian groups is presented. The key point for this method is the definition of a kind of newsymmetry moecular orbitals (SMOs). Any group ele...A novel method which makes full use of point groups including Abeian and nonAbelian groups is presented. The key point for this method is the definition of a kind of newsymmetry moecular orbitals (SMOs). Any group element in a moecular point group willtransform one SMO to another equlvalent SMO and no mixture among SMCh exists. The unique feature will be kept for one or two - electron integrals as well as the Slater determinantsconstructed using SMCh. Consequently, the character integrals and character detendnants(CDs) can be selected according to some simple rules. Based on this method, a preliminarycharacter single and doubly excited single reference CI (CD - SR - CISD) program is developed, and pilot calculations are performed and compared with GAUSSIAN - 94.For thehigh symmetry point group models and linear molecules our CD - CISD program costs lessCPU - time than G94 - CISD and the storage demand of CD - CISD is also remarkably alleviated.展开更多
基金supported by the Sanming Project of Medicine in Shenzhen[SZZYSM202206001]National Natural Science Foundation of China[82004320 and 82374383]+3 种基金Natural Science Foundation of Guangdong Province of China[2022A1515011710 and 2022A1515010679]Shenzhen Science and Technology Innovation Committee[JCYJ20220530141407017 and JCYJ20240813153619026]2024 High-quality Development Research Project of Shenzhen Bao’an Public Hospital[YNXM2024078]and Shenzhen Bao’an Chinese Medicine Hospital Research Program[BAZYY20220702].
文摘Background:“Qi deficiency”(a pathological state where the body’s vital energy(Qi)is insufficient or weakened,impairing physiological functions and diminishing the body’s ability to perform daily activities,defend against illness,and maintain homeostasis)syndrome is considered a critical syndrome in traditional Chinese medicine(TCM)and is associated with poor prognosis in heart failure(HF).This study investigates the clinical,metabolic,and transcriptomic differences between heart failure patients with and without Qi deficiency syndrome.Methods:56 heart failure patients were evaluated using a Qi deficiency syndrome scale and divided into Qi deficiency syndrome(QD)and non-Qi deficiency(non-QD)groups based on the median score.Clinical characteristics,including baseline N-terminal pro-B-type natriuretic peptide(NT-proBNP),left ventricular ejection fraction(LVEF),total diuretic use during hospitalization,and 90-day rehospitalization rates,were compared between the groups.Differentially expressed genes(DEGs)and differential metabolites were identified,followed by enrichment analyses and validation using qPCR and Western blot in AC16 cardiomyocytes.Results:QD patients exhibited significantly higher NT-proBNP levels,lower LVEF,and increased 90-day rehospitalization rates.Metabolomic profiling revealed lipid metabolism disruptions,notably in linoleic acid and phospholipid pathways.Transcriptomic analysis highlighted 17 DEGs,including CISD2,a critical mitochondrial regulator,which was downregulated in QD patients.Correlation analysis identified significant associations between DEGs(e.g.,CISD2,BPGM)and lipid metabolites such as PC(16:0/P-16:0).Functional knockdown of CISD2 in AC16 cells led to upregulation of lipid oxidation enzymes ALOX15 and CYP1A2,linking CISD2 dysfunction to lipid metabolic dysregulation.Conclusion:Qi deficiency is associated with more severe heart failure symptoms,worse prognosis,and distinct metabolic and transcriptomic profiles,particularly in lipid metabolism.CISD2 emerges as a potential therapeutic target,offering new avenues for integrating molecular insights with TCM approaches to optimize HF management.
基金supported by grants from the Taipei Institute of Pathology,Taiwan(TIP10102A)the Taipei City Hospital,Taiwan(TPCH-102-061 and TPCH-104-043)the Department of Health,Taipei City Government(10401-62-038)
文摘Multiple protective effects of curcumin in cases of spinal cord injuries（SCIs）：Curcumin[1,7-bis（4-hydroxy-3-methoxyphenyl）-1,6-heptadiene-3,5-dione]is a nonsteroidal,naturally occurring compound commonly utilized as a dietary pigment as well as a spice in India.It is obtained from curcuma longa in.
文摘CISD1,an outer mitochondrial membrane iron-sulfur cluster protein,regulates intracellular iron levels,oxidative stress,and mitochondrial dynamics,playing critical roles in cellular bioenergetics and redox homeostasis.Although CISD1 has been identified as a prognostic biomarker in specific cancers,its broader implications in tumorigenesis,cancer progression,and immunotherapy remain unclear.Given the heterogeneity of cancer and the need for robust biomarkers across cancers,this study conducts the first comprehensive pan-cancer analysis of CISD1 by evaluating its roles in cancer and treatment.We obtained and analyzed data from databases including TCGA,GTEx,THPA,GEPIA2.0,SangerBox,cBioPortal,TIMER2.0,CAMOIP,DAVID,SRPLOT,and TISIDB.Our findings reveal significant alterations in CISD1 expression at both transcriptional and translational levels,as well as gene mutations across multiple cancers,indicating its potential as a diagnostic biomarker and its involvement in cancer development and progression.CISD1 dysregulation is linked to poor clinical outcomes,as shown through its impact on patient prognosis.GO and KEGG analyses show that CISD1 plays critical roles in cellular bioenergetics.Notably,CISD1 expression is significantly correlated with tumor stemness indices,tumor mutation burden,microsatellite instability,and immune checkpoint proteins in multiple cancers,and altered CISD1 levels are also observed in patients responding to immunotherapy,further supporting its role not only in prognosis but also as a key predictor in immunotherapy responses and outcomes.Our findings demonstrate CISD1 as a reliable and promising diagnostic,prognostic,and immunotherapeutic biomarker for multiple cancers,emphasizing its crucial role in cancer biology and potential to guide personalized cancer therapies.
文摘A novel method which makes full use of point groups including Abeian and nonAbelian groups is presented. The key point for this method is the definition of a kind of newsymmetry moecular orbitals (SMOs). Any group element in a moecular point group willtransform one SMO to another equlvalent SMO and no mixture among SMCh exists. The unique feature will be kept for one or two - electron integrals as well as the Slater determinantsconstructed using SMCh. Consequently, the character integrals and character detendnants(CDs) can be selected according to some simple rules. Based on this method, a preliminarycharacter single and doubly excited single reference CI (CD - SR - CISD) program is developed, and pilot calculations are performed and compared with GAUSSIAN - 94.For thehigh symmetry point group models and linear molecules our CD - CISD program costs lessCPU - time than G94 - CISD and the storage demand of CD - CISD is also remarkably alleviated.
