钙调磷酸酶B蛋白(calcineurin B like protein, CBL)互作蛋白激酶(CBL-interacting protein kinase,CIPK)是一类Ser/Thr蛋白激酶,在植物生长发育和逆境胁迫响应过程中发挥重要作用。CIPK可自磷酸化或靶向磷酸化其他信号因子以响应生物...钙调磷酸酶B蛋白(calcineurin B like protein, CBL)互作蛋白激酶(CBL-interacting protein kinase,CIPK)是一类Ser/Thr蛋白激酶,在植物生长发育和逆境胁迫响应过程中发挥重要作用。CIPK可自磷酸化或靶向磷酸化其他信号因子以响应生物和非生物逆境胁迫,也可参与脱落酸(abscisic acid,ABA)、赤霉素(gibberellic acid, GA)、乙烯(ethylene, ETH)、水杨酸(salicylic acid, SA)等植物激素信号通路来调节植物生长发育过程。此外,CIPK通过与CBL蛋白特异性互作形成复合物对盐碱、干旱、极端低温及重金属等逆境胁迫做出应答反应。本文对植物CIPKs的发现、结构、分类、调控机制及其在逆境胁迫响应中的作用等方面研究成果进行综述,并对其未来研究方向进行展望,为农作物抗逆性遗传改良和生物育种提供了参考。展开更多
The complex of calcineurin B-like protein(CBL)and CBL-interacting protein kinase(CIPK)serves as key components in calcium-sensing,orchestrating various signals crucial for plant growth,development,and responses to bio...The complex of calcineurin B-like protein(CBL)and CBL-interacting protein kinase(CIPK)serves as key components in calcium-sensing,orchestrating various signals crucial for plant growth,development,and responses to biotic and abiotic stresses.However,the mechanism underlying the response of this module to cold stress and its role in flower development in wintersweet(Chimonanthus praecox)remains unclear.Through expression pattern analysis,calcium ion(Ca^(2+))concentration assays,correlation analysis,and linear regression analysis,we found that the[Ca^(2+)],along with CpCBL8 and CpCIPK9 expression levels in wintersweet flower buds(FBs),significantly decreased during the initial flowering stage when the chilling requirement reached 570 chill units(CU).Notably,there was a significant positive correlation between[Ca^(2+)]and CpCBL8 expression.Ca^(2+)increased the expression of Cp CBL8 and CpCIPK9 in FBs,causing a significant delay in the flowering of wintersweet.Furthermore,the function of CpCBL8 was studied using heterologous transformation.Overexpression of CpCBL8 significantly delayed flowering time and significantly reduced cold tolerance and altered the expression pattern of endogenous genes related to low-temperature stress and flower development in transgenic Arabidopsis thaliana.Additionally,transcriptome analysis of chilling-induced dormancy breaking and flower bud enlargement revealed that CpCBL8 and CpCIPK9 were negatively regulated by cold,and the expression pattern of endogenous genes related to flower development and cold stress in wintersweet were similar to that of in A.thaliana.Moreover,protein-protein interaction(PPI)analysis revealed that CpCBL8 and CpCIPK9 interacted in the plasma membrane and nucleus.On the basis of these findings,we speculated that the CpCBL8-CpCIPK9 module plays a crucial role in regulating responses to cold stress and flower development in wintersweet.This study elucidated molecular mechanisms through which the downregulation of the Ca^(2+)-induced CpCBL8-CpCIPK9 module results in dormancy breaking and enhances cold tolerance.This study provides valuable insights for the cultivation of new varieties of wintersweet with increased ornamental value and enhanced cold stress tolerance.展开更多
CIPK(CBL-interacting protein kinase)是近年来鉴定出的植物中特有的一类Ca2+传感器,与其互作的蛋白一起在植物特定的生长发育和应答胁迫过程中起重要作用。本研究从玉米中克隆到1个1338bp的ZmCIPK21基因,荧光定量PCR分析表明ZmCIPK21...CIPK(CBL-interacting protein kinase)是近年来鉴定出的植物中特有的一类Ca2+传感器,与其互作的蛋白一起在植物特定的生长发育和应答胁迫过程中起重要作用。本研究从玉米中克隆到1个1338bp的ZmCIPK21基因,荧光定量PCR分析表明ZmCIPK21与盐、ABA、高温等逆境胁迫相关;生物信息学分析表明ZmCIPK21基因组含有14个外显子和13个内含子,蛋白结构上具有CIPK所共有的N端激酶结构域和C端NAF保守结构域。与拟南芥CIPK家族进化分析结果显示ZmCIPK21与AtCIPK21具有较高同源性。展开更多
Ca2+是植物在不同发育阶段和响应多种复杂环境刺激的核心调控因子。CBL蛋白(Calcineurin B-likeproteins)和CIPK蛋白激酶(CBL-interacting protein kinases)通过相互作用解码特异的钙信号从而实现相应的生理功能。文章主要从CBL-CIPK信...Ca2+是植物在不同发育阶段和响应多种复杂环境刺激的核心调控因子。CBL蛋白(Calcineurin B-likeproteins)和CIPK蛋白激酶(CBL-interacting protein kinases)通过相互作用解码特异的钙信号从而实现相应的生理功能。文章主要从CBL-CIPK信号系统关键组分及相互作用,解码钙信号的机制,生理功能和调节机制等方面综述相关研究进展,并对未来的研究方向进行展望。展开更多
文摘钙调磷酸酶B蛋白(calcineurin B like protein, CBL)互作蛋白激酶(CBL-interacting protein kinase,CIPK)是一类Ser/Thr蛋白激酶,在植物生长发育和逆境胁迫响应过程中发挥重要作用。CIPK可自磷酸化或靶向磷酸化其他信号因子以响应生物和非生物逆境胁迫,也可参与脱落酸(abscisic acid,ABA)、赤霉素(gibberellic acid, GA)、乙烯(ethylene, ETH)、水杨酸(salicylic acid, SA)等植物激素信号通路来调节植物生长发育过程。此外,CIPK通过与CBL蛋白特异性互作形成复合物对盐碱、干旱、极端低温及重金属等逆境胁迫做出应答反应。本文对植物CIPKs的发现、结构、分类、调控机制及其在逆境胁迫响应中的作用等方面研究成果进行综述,并对其未来研究方向进行展望,为农作物抗逆性遗传改良和生物育种提供了参考。
基金funded by the Natural Science Foundation of Chongqing(CSTB2023NSCQ-MSX0236)Fundamental Research Funds for the Central Universities(SWU-XDJH202308)Earmarked Funds for the China Agriculture Research System(CARS-26)。
文摘The complex of calcineurin B-like protein(CBL)and CBL-interacting protein kinase(CIPK)serves as key components in calcium-sensing,orchestrating various signals crucial for plant growth,development,and responses to biotic and abiotic stresses.However,the mechanism underlying the response of this module to cold stress and its role in flower development in wintersweet(Chimonanthus praecox)remains unclear.Through expression pattern analysis,calcium ion(Ca^(2+))concentration assays,correlation analysis,and linear regression analysis,we found that the[Ca^(2+)],along with CpCBL8 and CpCIPK9 expression levels in wintersweet flower buds(FBs),significantly decreased during the initial flowering stage when the chilling requirement reached 570 chill units(CU).Notably,there was a significant positive correlation between[Ca^(2+)]and CpCBL8 expression.Ca^(2+)increased the expression of Cp CBL8 and CpCIPK9 in FBs,causing a significant delay in the flowering of wintersweet.Furthermore,the function of CpCBL8 was studied using heterologous transformation.Overexpression of CpCBL8 significantly delayed flowering time and significantly reduced cold tolerance and altered the expression pattern of endogenous genes related to low-temperature stress and flower development in transgenic Arabidopsis thaliana.Additionally,transcriptome analysis of chilling-induced dormancy breaking and flower bud enlargement revealed that CpCBL8 and CpCIPK9 were negatively regulated by cold,and the expression pattern of endogenous genes related to flower development and cold stress in wintersweet were similar to that of in A.thaliana.Moreover,protein-protein interaction(PPI)analysis revealed that CpCBL8 and CpCIPK9 interacted in the plasma membrane and nucleus.On the basis of these findings,we speculated that the CpCBL8-CpCIPK9 module plays a crucial role in regulating responses to cold stress and flower development in wintersweet.This study elucidated molecular mechanisms through which the downregulation of the Ca^(2+)-induced CpCBL8-CpCIPK9 module results in dormancy breaking and enhances cold tolerance.This study provides valuable insights for the cultivation of new varieties of wintersweet with increased ornamental value and enhanced cold stress tolerance.
文摘大多数信号转导过程都伴随着细胞内钙离子浓度变化,CBL/CIPK信号系统是近几年在高等植物中发现的一类依赖于钙离子的信号系统,包括感知钙离子浓度变化的CBL蛋白(calcineurin B-like proteins)和与之互作的CIPK蛋白(CBL-interacting protein kinase)。研究表明CBL/CIPK信号系统在植物对逆境应答过程中起重要作用。在介绍CBL和CIPK的结构和特征基础上对不同植物CBL/CIPK信号系统在逆境应答中的研究现状况进行了综述,以期为基因工程改良作物抗逆育种提供新的思路和种质资源。
文摘CIPK(CBL-interacting protein kinase)是近年来鉴定出的植物中特有的一类Ca2+传感器,与其互作的蛋白一起在植物特定的生长发育和应答胁迫过程中起重要作用。本研究从玉米中克隆到1个1338bp的ZmCIPK21基因,荧光定量PCR分析表明ZmCIPK21与盐、ABA、高温等逆境胁迫相关;生物信息学分析表明ZmCIPK21基因组含有14个外显子和13个内含子,蛋白结构上具有CIPK所共有的N端激酶结构域和C端NAF保守结构域。与拟南芥CIPK家族进化分析结果显示ZmCIPK21与AtCIPK21具有较高同源性。
文摘Ca2+是植物在不同发育阶段和响应多种复杂环境刺激的核心调控因子。CBL蛋白(Calcineurin B-likeproteins)和CIPK蛋白激酶(CBL-interacting protein kinases)通过相互作用解码特异的钙信号从而实现相应的生理功能。文章主要从CBL-CIPK信号系统关键组分及相互作用,解码钙信号的机制,生理功能和调节机制等方面综述相关研究进展,并对未来的研究方向进行展望。
