目的探寻雪卡毒素研究进展,了解国内外研究现状及其发展趋势。方法基于Web of Science(WoS)核心合集数据库和中国知网(CNKI)雪卡毒素研究文献,通过CiteSpace 6.3.1软件绘制可视化科学知识图谱进行文献计量分析。结果国内外每年对雪卡毒...目的探寻雪卡毒素研究进展,了解国内外研究现状及其发展趋势。方法基于Web of Science(WoS)核心合集数据库和中国知网(CNKI)雪卡毒素研究文献,通过CiteSpace 6.3.1软件绘制可视化科学知识图谱进行文献计量分析。结果国内外每年对雪卡毒素的研究数量总体呈来回波动趋势。国外主要科研团队来自日本、美国等高校和科研院所,澳大利亚和法国等国家起到重要的链接作用;国内主要研究机构是深圳市疾病预防控制中心、广东工业大学等研究机构。国内外研究者多为小组研究,国内小组间合作较少。随着雪卡毒素研究工作的开展,其研究热点随之变化,甲藻、雪卡毒素中毒、基因调控等是今后的研究热点。结论该研究为了解雪卡毒素研究现状及发展趋势提供了参考依据。展开更多
Ohjective To review the clinical features and laboratory investigations of ciguatera patients in Hong Kong between 2004 and 2007 in order to show the timely sampling of implicated fish from ciguatera victims and appli...Ohjective To review the clinical features and laboratory investigations of ciguatera patients in Hong Kong between 2004 and 2007 in order to show the timely sampling of implicated fish from ciguatera victims and application of validated mouse bioassay for confirming suspected clinical cases of ciguatera. Methods Diagnosis of the ciguatera victims was based on history of coral fish consumption and clinical presentations stated in official guidelines for clinical diagnosis of ciguatera fish poisoning in Hong Kong. Food remnants of coral fish samples were collected swiftly from ciguatera victims between 2004 and 2007 for ciguatoxins （CTXs） analysis. Results Major clinical symptoms in ciguatera patients included gastrointestinal and neurological effects including limb numbness and diarrhoea, which developed at 0.5 to 15 hours after consumption of fish. In most cases, neurological symptoms were more common than gastrointestinal symptoms. A broad range of attack rate （10%-100%） was observed in each ciguatera outbreak. Validated mouse bioassay on ether extracts of the food remnant samples confirmed that all were CTXs-positive （〈0.5 - 4.3 MU/20 mg ether extract） and directly linked to the corresponding ciguatera cases. Conclusion Consistency between clinical and laboratory analysis for ciguatera poisoning illustrates the application of laboratory mouse bioassay in a timely fashion for confirming ciguatera poisoning cases and implementing effective public health measures. With further improvement in laboratory techniques, features of ciguatera fish poisoning cases can be better defined, Further studies are needed to determine the risk of each class of CTXs （Pacific-, Indian- and Caribbean-CTXs） in Hong Kong.展开更多
文摘目的探寻雪卡毒素研究进展,了解国内外研究现状及其发展趋势。方法基于Web of Science(WoS)核心合集数据库和中国知网(CNKI)雪卡毒素研究文献,通过CiteSpace 6.3.1软件绘制可视化科学知识图谱进行文献计量分析。结果国内外每年对雪卡毒素的研究数量总体呈来回波动趋势。国外主要科研团队来自日本、美国等高校和科研院所,澳大利亚和法国等国家起到重要的链接作用;国内主要研究机构是深圳市疾病预防控制中心、广东工业大学等研究机构。国内外研究者多为小组研究,国内小组间合作较少。随着雪卡毒素研究工作的开展,其研究热点随之变化,甲藻、雪卡毒素中毒、基因调控等是今后的研究热点。结论该研究为了解雪卡毒素研究现状及发展趋势提供了参考依据。
基金科技部973项目2013CB956503号+7 种基金海洋公益性项目201005006-01号国家自然科学基金项目41106090号中央高校基本科研业务费专项资金21612401号U.S.Centers for Disease Control and PreventionU01 EH000421号
文摘Ohjective To review the clinical features and laboratory investigations of ciguatera patients in Hong Kong between 2004 and 2007 in order to show the timely sampling of implicated fish from ciguatera victims and application of validated mouse bioassay for confirming suspected clinical cases of ciguatera. Methods Diagnosis of the ciguatera victims was based on history of coral fish consumption and clinical presentations stated in official guidelines for clinical diagnosis of ciguatera fish poisoning in Hong Kong. Food remnants of coral fish samples were collected swiftly from ciguatera victims between 2004 and 2007 for ciguatoxins （CTXs） analysis. Results Major clinical symptoms in ciguatera patients included gastrointestinal and neurological effects including limb numbness and diarrhoea, which developed at 0.5 to 15 hours after consumption of fish. In most cases, neurological symptoms were more common than gastrointestinal symptoms. A broad range of attack rate （10%-100%） was observed in each ciguatera outbreak. Validated mouse bioassay on ether extracts of the food remnant samples confirmed that all were CTXs-positive （〈0.5 - 4.3 MU/20 mg ether extract） and directly linked to the corresponding ciguatera cases. Conclusion Consistency between clinical and laboratory analysis for ciguatera poisoning illustrates the application of laboratory mouse bioassay in a timely fashion for confirming ciguatera poisoning cases and implementing effective public health measures. With further improvement in laboratory techniques, features of ciguatera fish poisoning cases can be better defined, Further studies are needed to determine the risk of each class of CTXs （Pacific-, Indian- and Caribbean-CTXs） in Hong Kong.
