A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T gr...A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0-8.0, at 28-30 ℃ and in the presence of NaCI concentrations from 0.0% to 0.5%. 16S rRNA gene sequence analysis indicated that strain 25-1m belongs to the genus Chryseobacterium within the family Flavo- bacteriaceae and is related most closely to C. camis G81m （96.4% similarity）, C. lathyri RBA2-6T （95.8% similarity）, and C. zeae JM1085T （95.8% similarity）. Its genomic DNA G＋C molar composition was 36.2%. The major cellular fatty acids were iso-C15：0 （44.0%）, iso-C17：0 3OH （19.8%） and C16：1 ω7c/16：1 ω6c （12.7%）. The only isoprenoid quinone was menaquinone 6 （MK-6）. The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1m and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T （ccmoc AB2015133T=DSM 100396T）.展开更多
Purpose: Since Chryseobacterium indologenes (C. indologenes) is a rare human pathogen, its clinical significance has not yet been fully established. C. indologenes contamination of medical devices involving fluids and...Purpose: Since Chryseobacterium indologenes (C. indologenes) is a rare human pathogen, its clinical significance has not yet been fully established. C. indologenes contamination of medical devices involving fluids and of surgical implants has led to an increasing number of serious infectious reported in recent years, mainly in patients in extremes ages and an immunocompromised state. In this study, we describe the clinical and microbiological characteristics of seven adult patients with C. indologenes bacteremia treated in a tertiary medical center in Israel over a six-year period. Methods: Adult patients hospitalized in Rabin Medical Center, Israel, with a blood culture positive for C. indologenes during the period 2009-2014 were identified retrospectively and their medical records were reviewed. Results: Seven episodes of C. indologenes bacteremia in seven patients were identified during the study period. Five patients were females;the mean age was 76.8 years (41 - 92). Serious underlying conditions were present in all patients. All patients but one, presented after a recent invasive healthcare related intervention. Two patients required mechanical ventilation. Two patients died. All but one isolates were susceptible to ciprofloxacin. Conclusions: Despite our limited number of cases, to the best of our knowledge, our study serves as the largest cohort of adult patients with C. indologenes bacteremia reported in recent years.展开更多
Objective:To investigate the clinical and epidemiological features and outcome of Chryseobacterium and Elizabethkingia spp.infections in children,together with antimicrobial susceptibilities.Methods:This retrospective...Objective:To investigate the clinical and epidemiological features and outcome of Chryseobacterium and Elizabethkingia spp.infections in children,together with antimicrobial susceptibilities.Methods:This retrospective study was conducted at a tertiary pediatric hospital in Turkey.All patients infected with Chryseobacterium/Elizabethkingia spp.among those presenting to Ankara City Hospital between March 2014 and March 2022 were included.Results:A total of 49 cases were included and 29 cases were identified as Elizabethkingia.The median age was 14(0.2-185.0)months.The majority(89.8%)of these patients had an underlying disease,including malignancy(42.9%).Bacteremia(46.9%)and central line-associated bloodstream infection(28.6%)were the most common infections.The thirty-day all-cause mortality rate was 12.2%.The most commonly used antibiotics were ciprofloxacin and trimethoprim-sulfamethoxazole(TMP-SMX).Forty-five(91.8%)isolates were susceptible to ciprofloxacin,44(91.6%)to TMP-SMX,and 21(87.5%)to levofloxacin.Conclusions:Chryseobacterium and Elizabethkingia spp.are emergent,nosocomial pathogens and the majority of cases were older than the neonatal period.They were mainly seen in patients with long hospital stays,indwelling devices,and those who have received antibiotics within the last month,especially carbapenems.In addition,they were associated with bloodstream infection and malignancy.The most commonly useful antibiotics according to the resistance patterns were ciprofloxacin and TMP-SMX.展开更多
Two dominant strains of bacteria Lb18-01 and Lb18-02 were isolated and purified from scabies,liver and intestine of diseased Peodiscus sinensis.By artificial infection test,the two strains were verified as pathogenic ...Two dominant strains of bacteria Lb18-01 and Lb18-02 were isolated and purified from scabies,liver and intestine of diseased Peodiscus sinensis.By artificial infection test,the two strains were verified as pathogenic strains with similar characters to that of natural infectious cases.The strains Lb18-01 and Lb18-02 showed strong pathogenicity to healthy P.sinensis in the artificial infection experiment,so they were the pathogenic strain of the disease.According to the morphology,physicochemical characteristics,16 S rDNA sequence analysis and phylogenetic tree clustering,the pathogenic strains Lb18-01 and Lb18-02 were identified as Proteus vulgaris and Chryseobacterium meningosepticum which were tolerant to 20 drugs such as penicillin,tetracycline and ampicillin.Histopathological observation on diseased P.sinensis showed the pathological symptoms of sepsis such as hemorrhage and congestion of liver,spleen and intestine,and glomerular disintegration.展开更多
[Objective] The aim was to explore the relationship between alage-lysing bacterium and Anabaena flosaquae so as to provide reference for the control of bloom. [Method] An algae-lysing bacterium strain named S7 was iso...[Objective] The aim was to explore the relationship between alage-lysing bacterium and Anabaena flosaquae so as to provide reference for the control of bloom. [Method] An algae-lysing bacterium strain named S7 was isolated from eu- trophic river. The lyric efficiency and performing mode of S7 strain to Anabaena flos- aquae was studied. Influence of different environmental factors and the relationship between S7 strain and Anabaena flosaquae was also studied, and then the bacteri- um strain was physiologically identified. [Result] More than 90% of Anabaena flos- aquae had been removed by 7 d when the volume ratio of medium to algae solu- tion was 30%, the pH was 9 and the temperature was 35 ℃. These results also showed that a mutual inhibit relationship existed between S7 strain and Anabaena flos-aquae. The S7 strain killed the algae by indirectly through certain lyric agents in absence of direct contact with the target but by secreting metabolites. Moreover, these lyric agents also had the thermostability. 16SrDNA sequence analysis showed that S7 strain belonged to Chryseobacterium sp. [Conclusion] The examined Poly-p proved that S7 strain is polyphosphate accumulating bacteria （PAOs） and has better lyric efficiency.展开更多
对患病中华鳖(Pelodiscus sinensis)进行病原分离、鉴定及药敏实验,从患病中华鳖皮肤、肝肾脾重要器官分离纯化病原菌,经理化特性测定及16S r RNA序列分析对其进行鉴定及人工感染试验,并利用K-B及二倍稀释法进行药敏特性分析。结果表明...对患病中华鳖(Pelodiscus sinensis)进行病原分离、鉴定及药敏实验,从患病中华鳖皮肤、肝肾脾重要器官分离纯化病原菌,经理化特性测定及16S r RNA序列分析对其进行鉴定及人工感染试验,并利用K-B及二倍稀释法进行药敏特性分析。结果表明分离株J22是为中华鳖腐皮病病原,其对中华鳖的LD50为3.30×104 CFU/g。J22株理化特性与产吲哚金黄杆菌(Chryseobacterium indologenes)一致,16S r RNA序列与产吲哚金黄杆菌同源性为99%,综合判定J22株是产吲哚金黄杆菌。分离株对新霉素、庆大霉素及阿莫西林等12种抗生素高度敏感,对氟苯尼考及多西环素等抗生素耐药;二氧化氯、漂白粉及高铁酸钾对分离株消毒效果较好。分离菌株J22是中华鳖病原菌,养殖时可选用庆大霉素、新霉素或者阿莫西林内服,配合使用二氧化氯、漂白粉及高铁酸钾等外用进行防控。展开更多
[目的]分离并鉴定棘胸蛙歪头病病原。[方法]从患歪头病棘胸蛙(Quasipaa spinosa)体内分离到致病菌,分别采用4种不同方式进行人工感染试验,通过形态观察、生理生化试验、16S r DNA测序等方法进行鉴定,并采用抑菌圈研究该菌株对12种抗生...[目的]分离并鉴定棘胸蛙歪头病病原。[方法]从患歪头病棘胸蛙(Quasipaa spinosa)体内分离到致病菌,分别采用4种不同方式进行人工感染试验,通过形态观察、生理生化试验、16S r DNA测序等方法进行鉴定,并采用抑菌圈研究该菌株对12种抗生素的敏感性。[结果]除口服方式外,肌肉注射、皮肤损伤浸泡、皮肤不损伤浸泡感染途径均能使棘胸蛙发病并导致死亡,且对蛙有较强的致病性。通过生理生化试验和16S r DNA测序等方法鉴定致病菌为脑膜炎败血金黄杆菌(Chryseobacterium meningosepticum)。药敏试验结果表明:该致病菌对万古霉素、头孢哌酮、庆大霉素和红霉素高度敏感,而对吡哌酸、链霉素、四环素、诺氟沙星、青霉素、新霉素、林可霉素、先锋霉素不敏感。[结论]棘胸蛙歪头病病原为脑膜炎败血金黄杆菌。展开更多
基金supported by the Chengdu Giant Panda Breeding Research Foundation(No.CPF2010-06)the National Key Technology R&D Program of China(No.2012BAC01B06)
文摘A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0-8.0, at 28-30 ℃ and in the presence of NaCI concentrations from 0.0% to 0.5%. 16S rRNA gene sequence analysis indicated that strain 25-1m belongs to the genus Chryseobacterium within the family Flavo- bacteriaceae and is related most closely to C. camis G81m （96.4% similarity）, C. lathyri RBA2-6T （95.8% similarity）, and C. zeae JM1085T （95.8% similarity）. Its genomic DNA G＋C molar composition was 36.2%. The major cellular fatty acids were iso-C15：0 （44.0%）, iso-C17：0 3OH （19.8%） and C16：1 ω7c/16：1 ω6c （12.7%）. The only isoprenoid quinone was menaquinone 6 （MK-6）. The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1m and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T （ccmoc AB2015133T=DSM 100396T）.
文摘Purpose: Since Chryseobacterium indologenes (C. indologenes) is a rare human pathogen, its clinical significance has not yet been fully established. C. indologenes contamination of medical devices involving fluids and of surgical implants has led to an increasing number of serious infectious reported in recent years, mainly in patients in extremes ages and an immunocompromised state. In this study, we describe the clinical and microbiological characteristics of seven adult patients with C. indologenes bacteremia treated in a tertiary medical center in Israel over a six-year period. Methods: Adult patients hospitalized in Rabin Medical Center, Israel, with a blood culture positive for C. indologenes during the period 2009-2014 were identified retrospectively and their medical records were reviewed. Results: Seven episodes of C. indologenes bacteremia in seven patients were identified during the study period. Five patients were females;the mean age was 76.8 years (41 - 92). Serious underlying conditions were present in all patients. All patients but one, presented after a recent invasive healthcare related intervention. Two patients required mechanical ventilation. Two patients died. All but one isolates were susceptible to ciprofloxacin. Conclusions: Despite our limited number of cases, to the best of our knowledge, our study serves as the largest cohort of adult patients with C. indologenes bacteremia reported in recent years.
文摘Objective:To investigate the clinical and epidemiological features and outcome of Chryseobacterium and Elizabethkingia spp.infections in children,together with antimicrobial susceptibilities.Methods:This retrospective study was conducted at a tertiary pediatric hospital in Turkey.All patients infected with Chryseobacterium/Elizabethkingia spp.among those presenting to Ankara City Hospital between March 2014 and March 2022 were included.Results:A total of 49 cases were included and 29 cases were identified as Elizabethkingia.The median age was 14(0.2-185.0)months.The majority(89.8%)of these patients had an underlying disease,including malignancy(42.9%).Bacteremia(46.9%)and central line-associated bloodstream infection(28.6%)were the most common infections.The thirty-day all-cause mortality rate was 12.2%.The most commonly used antibiotics were ciprofloxacin and trimethoprim-sulfamethoxazole(TMP-SMX).Forty-five(91.8%)isolates were susceptible to ciprofloxacin,44(91.6%)to TMP-SMX,and 21(87.5%)to levofloxacin.Conclusions:Chryseobacterium and Elizabethkingia spp.are emergent,nosocomial pathogens and the majority of cases were older than the neonatal period.They were mainly seen in patients with long hospital stays,indwelling devices,and those who have received antibiotics within the last month,especially carbapenems.In addition,they were associated with bloodstream infection and malignancy.The most commonly useful antibiotics according to the resistance patterns were ciprofloxacin and TMP-SMX.
基金Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals(2018TP1027)Key R&D Projects of Hunan Province(2018NK2074).
文摘Two dominant strains of bacteria Lb18-01 and Lb18-02 were isolated and purified from scabies,liver and intestine of diseased Peodiscus sinensis.By artificial infection test,the two strains were verified as pathogenic strains with similar characters to that of natural infectious cases.The strains Lb18-01 and Lb18-02 showed strong pathogenicity to healthy P.sinensis in the artificial infection experiment,so they were the pathogenic strain of the disease.According to the morphology,physicochemical characteristics,16 S rDNA sequence analysis and phylogenetic tree clustering,the pathogenic strains Lb18-01 and Lb18-02 were identified as Proteus vulgaris and Chryseobacterium meningosepticum which were tolerant to 20 drugs such as penicillin,tetracycline and ampicillin.Histopathological observation on diseased P.sinensis showed the pathological symptoms of sepsis such as hemorrhage and congestion of liver,spleen and intestine,and glomerular disintegration.
基金Supported by Ecological Environment Construction and Protection Demonstration Project of Three Gorges~~
文摘[Objective] The aim was to explore the relationship between alage-lysing bacterium and Anabaena flosaquae so as to provide reference for the control of bloom. [Method] An algae-lysing bacterium strain named S7 was isolated from eu- trophic river. The lyric efficiency and performing mode of S7 strain to Anabaena flos- aquae was studied. Influence of different environmental factors and the relationship between S7 strain and Anabaena flosaquae was also studied, and then the bacteri- um strain was physiologically identified. [Result] More than 90% of Anabaena flos- aquae had been removed by 7 d when the volume ratio of medium to algae solu- tion was 30%, the pH was 9 and the temperature was 35 ℃. These results also showed that a mutual inhibit relationship existed between S7 strain and Anabaena flos-aquae. The S7 strain killed the algae by indirectly through certain lyric agents in absence of direct contact with the target but by secreting metabolites. Moreover, these lyric agents also had the thermostability. 16SrDNA sequence analysis showed that S7 strain belonged to Chryseobacterium sp. [Conclusion] The examined Poly-p proved that S7 strain is polyphosphate accumulating bacteria （PAOs） and has better lyric efficiency.
文摘对患病中华鳖(Pelodiscus sinensis)进行病原分离、鉴定及药敏实验,从患病中华鳖皮肤、肝肾脾重要器官分离纯化病原菌,经理化特性测定及16S r RNA序列分析对其进行鉴定及人工感染试验,并利用K-B及二倍稀释法进行药敏特性分析。结果表明分离株J22是为中华鳖腐皮病病原,其对中华鳖的LD50为3.30×104 CFU/g。J22株理化特性与产吲哚金黄杆菌(Chryseobacterium indologenes)一致,16S r RNA序列与产吲哚金黄杆菌同源性为99%,综合判定J22株是产吲哚金黄杆菌。分离株对新霉素、庆大霉素及阿莫西林等12种抗生素高度敏感,对氟苯尼考及多西环素等抗生素耐药;二氧化氯、漂白粉及高铁酸钾对分离株消毒效果较好。分离菌株J22是中华鳖病原菌,养殖时可选用庆大霉素、新霉素或者阿莫西林内服,配合使用二氧化氯、漂白粉及高铁酸钾等外用进行防控。
文摘[目的]分离并鉴定棘胸蛙歪头病病原。[方法]从患歪头病棘胸蛙(Quasipaa spinosa)体内分离到致病菌,分别采用4种不同方式进行人工感染试验,通过形态观察、生理生化试验、16S r DNA测序等方法进行鉴定,并采用抑菌圈研究该菌株对12种抗生素的敏感性。[结果]除口服方式外,肌肉注射、皮肤损伤浸泡、皮肤不损伤浸泡感染途径均能使棘胸蛙发病并导致死亡,且对蛙有较强的致病性。通过生理生化试验和16S r DNA测序等方法鉴定致病菌为脑膜炎败血金黄杆菌(Chryseobacterium meningosepticum)。药敏试验结果表明:该致病菌对万古霉素、头孢哌酮、庆大霉素和红霉素高度敏感,而对吡哌酸、链霉素、四环素、诺氟沙星、青霉素、新霉素、林可霉素、先锋霉素不敏感。[结论]棘胸蛙歪头病病原为脑膜炎败血金黄杆菌。
