A new GC/MS method for detection and identification of 19 anabolic steroids in human urine is presented.The procedure involves adsorption and isolation on a macroporous XAD-2 resin,enzymatic hydrolysis,alkaline extrac...A new GC/MS method for detection and identification of 19 anabolic steroids in human urine is presented.The procedure involves adsorption and isolation on a macroporous XAD-2 resin,enzymatic hydrolysis,alkaline extraction,derivatization,GC separation and MS detec- tion.Gas chromatographic-mass spectrometric data illustrate artifacts arising from enzymatic hydrolysis of steroid glucuronides and the structural characterization of their metabolites. Using this method,metabolic studies of these steroids in human urine were made after their ingestion by normal and healthy male volunteers.This method was proven to be suitable for large-scale routine analysis of anabolic steroids and was used successfully in passing the doping control test held by the Medical Commission of the International Olympic Committee.展开更多
Preliminary phytochemical study of methanol extracts from Vernonanthura patens’s leaves located in Marcabelí (El Oro province), Ecuador. The methodology consisted of chromatographic column separations with incre...Preliminary phytochemical study of methanol extracts from Vernonanthura patens’s leaves located in Marcabelí (El Oro province), Ecuador. The methodology consisted of chromatographic column separations with increasing polarity solvents and the analysis of fractions by Gas Chromatography-Mass Spectrometry coupled system (GC-MS). The structure of 53 compounds was proposed. Analyzed V. patens species showed the presence of terpene and aliphatic hydrocarbons, free fatty acids and their methyl and ethyl esters, oxygen sesqui, triterpenoids and sugars. For ecuadorian species, there are no records of chemical studies.展开更多
文摘A new GC/MS method for detection and identification of 19 anabolic steroids in human urine is presented.The procedure involves adsorption and isolation on a macroporous XAD-2 resin,enzymatic hydrolysis,alkaline extraction,derivatization,GC separation and MS detec- tion.Gas chromatographic-mass spectrometric data illustrate artifacts arising from enzymatic hydrolysis of steroid glucuronides and the structural characterization of their metabolites. Using this method,metabolic studies of these steroids in human urine were made after their ingestion by normal and healthy male volunteers.This method was proven to be suitable for large-scale routine analysis of anabolic steroids and was used successfully in passing the doping control test held by the Medical Commission of the International Olympic Committee.
文摘Preliminary phytochemical study of methanol extracts from Vernonanthura patens’s leaves located in Marcabelí (El Oro province), Ecuador. The methodology consisted of chromatographic column separations with increasing polarity solvents and the analysis of fractions by Gas Chromatography-Mass Spectrometry coupled system (GC-MS). The structure of 53 compounds was proposed. Analyzed V. patens species showed the presence of terpene and aliphatic hydrocarbons, free fatty acids and their methyl and ethyl esters, oxygen sesqui, triterpenoids and sugars. For ecuadorian species, there are no records of chemical studies.
