目的：评价Chrom ID ESBL产色培养基检测产超广谱β-内酰胺酶肠杆菌科菌株的可靠性．方法：共使用2组肠杆菌科菌株对平板进行评估，第1组细菌是我院2010-04/2013-03各病区和门诊临床分离送检的样本，共982株，第2组细菌是本地区历往年...目的：评价Chrom ID ESBL产色培养基检测产超广谱β-内酰胺酶肠杆菌科菌株的可靠性．方法：共使用2组肠杆菌科菌株对平板进行评估，第1组细菌是我院2010-04/2013-03各病区和门诊临床分离送检的样本，共982株，第2组细菌是本地区历往年分离培养，且确定产酶特性的肠杆菌科细菌20株．结果：Chrom ID ESBL显色平板可快速有效的筛查肠杆菌科细菌是否产ESBLs．结论：Chrom ID ESBL产色培养基是一种快速可靠的对产ESBLs的肠杆菌科菌株进行筛选的产色培养基．展开更多
The photonic characteristics of chromophore-containing metal-organic frameworks(MOFs)have led to extensive photophysical studies in an effort to capitalize on the potency of precisely controlled chromophore ensembles....The photonic characteristics of chromophore-containing metal-organic frameworks(MOFs)have led to extensive photophysical studies in an effort to capitalize on the potency of precisely controlled chromophore ensembles.Several examples have laid the foundation that demonstrates how photophysical properties of chromophores can be manipulated by tuning their communications(interactions)through integration within a MOF matrix.The main focus of this review is on harnessing the versatile MOF platform to accentuate the photophysical properties of integrated chromophores.In particular,this review will highlight chromophore dynamics that enhance,alter,or tune the photoluminescence response of single-and multi-chromophore-containing scaffolds,as well as alignment-guided anisotropic fluorescence.Building upon this groundwork,utilization of a hybrid crystalline motif can induce preferential orientation of chromophores resulting in enhanced communication and tailored behavior compared to randomly oriented emissive molecules.Moreover,frameworks that produce upconverted emission via sensitized triplet-triplet annihilation(sTTA),excited-state absorption(ESA),energy transfer upconversion(ETU),multi-photon absorption(MPA),or second-harmonic generation(SHG)can invoke dynamic control of material properties using photochromic linkers and will be discussed herein with a focus on the effects of chromophore alignment.Integration within a framework is a vehicle tofuse chromophores into solid-state platforms,opening an avenue for chromophore utilization in applications such as portable electronics that require solids or thin films.For those reasons,the design of chromophore-containing MOFs with desirable properties that rely on the alignment and communication of hundreds of chromophores within a single platform is a pressing demand for the development of futuristic technologies.展开更多
The mass-screening of gastric cancer has been carried out for years in Shanghai. A huge number of data and samples have been accumulated in this work. But there are still difficulties in the diagnosis of gastric cance...The mass-screening of gastric cancer has been carried out for years in Shanghai. A huge number of data and samples have been accumulated in this work. But there are still difficulties in the diagnosis of gastric cancer in early stage. The diagnosis of展开更多
Interaction between two phases exists whether in reversed phase thin-layer chromatography or normal phase thin-layer chromatography. But the strength of interaction between the two phases is different when chromatogra...Interaction between two phases exists whether in reversed phase thin-layer chromatography or normal phase thin-layer chromatography. But the strength of interaction between the two phases is different when chromatographic systems are changed. When the strength of interaction between the two phases is too small to展开更多
Background: Colonization with methicillin-resistant Staphylococcus aureus(MRSA) poses a hygiene risk that does not spare field hospitals or military medical field camps during military deployments. Diagnostic options ...Background: Colonization with methicillin-resistant Staphylococcus aureus(MRSA) poses a hygiene risk that does not spare field hospitals or military medical field camps during military deployments. Diagnostic options for unambiguously identifying MRSA isolates are usually scarce in military environments. In this study, we assessed the stepwise application of two different selective agars for the specific identification of MRSA in screening analyses.Methods: Nasal swabs from 1,541 volunteers were subjected to thioglycollate broth enrichment and subsequently screened on CHROMagar MRSA selective agar for the identification of MRSA. The MRSA identity of suspiciouslooking colonies was confirmed afterwards or excluded by another selective agar, chrom ID MRSA. All isolates from the selective agars with MRSA-specific colony morphology were identified by biochemical methods and mass spectrometry.Results: The initial CHROMagar MRSA screening identified suspicious colonies in 36 out of 1541 samples. A total of 25 of these 36 isolates showed MRSA-like growth on chrom ID agar. Out of these 25 isolates, 24 were confirmed as MRSA, while one isolate was identified as Staphylococcus kloosii. From the 11 strains that did not show suspicious growth on chrom ID agar, 3 were methicillin-sensitive Staphylococcus aureus(MSSA, with one instance of cocolonization with Corynebacterium spp.), 2 were confirmed as MRSA(with 1 instance of co-colonization with MSSA), 2 were lost during passaging and could not be re-cultured, one could not be identified by the applied approaches, and the remaining 3 strains were identified as Staphylococcus saprophyticus, Staphylococcus hominis(co-colonized with Macrococcus caseolyticus) and Staphylococcus cohnii, respectively.Conclusion: The application of the selective agar CHROMagar MRSA alone proved to be too non-specific to allow for a reliable diagnosis of the presence of MRSA. The combined use of two selective agars in a stepwise approach reduced this non-specificity with an acceptably low loss of sensitivity. Accordingly, such a stepwise screening approach might be an option for resource-restricted military medical field camps.展开更多
中药首乌及其复方具有降血脂、抗动脉硬化、保护肝脏、抗菌、提高免疫功能、抗衰老等作用。近年来,对它的抗肿瘤作用颇有研究。但对其诱变性和抗诱变作用研究,尚未见报道。本实验以复方首乌片为样品,应用小鼠骨髓细胞染色体畸变试验(chr...中药首乌及其复方具有降血脂、抗动脉硬化、保护肝脏、抗菌、提高免疫功能、抗衰老等作用。近年来,对它的抗肿瘤作用颇有研究。但对其诱变性和抗诱变作用研究,尚未见报道。本实验以复方首乌片为样品,应用小鼠骨髓细胞染色体畸变试验(chrom-some aberration;CA)和骨髓多染红细胞微核试验(micronucleus of polychromaticerythrocyte;MNPCE)二项遗传指标,对该药进行诱变性和抗诱变性研究。展开更多
文摘目的：评价Chrom ID ESBL产色培养基检测产超广谱β-内酰胺酶肠杆菌科菌株的可靠性．方法：共使用2组肠杆菌科菌株对平板进行评估，第1组细菌是我院2010-04/2013-03各病区和门诊临床分离送检的样本，共982株，第2组细菌是本地区历往年分离培养，且确定产酶特性的肠杆菌科细菌20株．结果：Chrom ID ESBL显色平板可快速有效的筛查肠杆菌科细菌是否产ESBLs．结论：Chrom ID ESBL产色培养基是一种快速可靠的对产ESBLs的肠杆菌科菌株进行筛选的产色培养基．
基金the NSF CAREER Award(DM R-1553634)a Cottrell Scholar Award from the Research Corporation for Science Advancement.N.B.S.also acknowledges support from the Sloan Research Fellowship provided by the Alfred P.Sloan Foundation and the Dreyfus Teaching-Scholar Award supported by the Dreyflis Foundation.
文摘The photonic characteristics of chromophore-containing metal-organic frameworks(MOFs)have led to extensive photophysical studies in an effort to capitalize on the potency of precisely controlled chromophore ensembles.Several examples have laid the foundation that demonstrates how photophysical properties of chromophores can be manipulated by tuning their communications(interactions)through integration within a MOF matrix.The main focus of this review is on harnessing the versatile MOF platform to accentuate the photophysical properties of integrated chromophores.In particular,this review will highlight chromophore dynamics that enhance,alter,or tune the photoluminescence response of single-and multi-chromophore-containing scaffolds,as well as alignment-guided anisotropic fluorescence.Building upon this groundwork,utilization of a hybrid crystalline motif can induce preferential orientation of chromophores resulting in enhanced communication and tailored behavior compared to randomly oriented emissive molecules.Moreover,frameworks that produce upconverted emission via sensitized triplet-triplet annihilation(sTTA),excited-state absorption(ESA),energy transfer upconversion(ETU),multi-photon absorption(MPA),or second-harmonic generation(SHG)can invoke dynamic control of material properties using photochromic linkers and will be discussed herein with a focus on the effects of chromophore alignment.Integration within a framework is a vehicle tofuse chromophores into solid-state platforms,opening an avenue for chromophore utilization in applications such as portable electronics that require solids or thin films.For those reasons,the design of chromophore-containing MOFs with desirable properties that rely on the alignment and communication of hundreds of chromophores within a single platform is a pressing demand for the development of futuristic technologies.
文摘The mass-screening of gastric cancer has been carried out for years in Shanghai. A huge number of data and samples have been accumulated in this work. But there are still difficulties in the diagnosis of gastric cancer in early stage. The diagnosis of
文摘Interaction between two phases exists whether in reversed phase thin-layer chromatography or normal phase thin-layer chromatography. But the strength of interaction between the two phases is different when chromatographic systems are changed. When the strength of interaction between the two phases is too small to
文摘Background: Colonization with methicillin-resistant Staphylococcus aureus(MRSA) poses a hygiene risk that does not spare field hospitals or military medical field camps during military deployments. Diagnostic options for unambiguously identifying MRSA isolates are usually scarce in military environments. In this study, we assessed the stepwise application of two different selective agars for the specific identification of MRSA in screening analyses.Methods: Nasal swabs from 1,541 volunteers were subjected to thioglycollate broth enrichment and subsequently screened on CHROMagar MRSA selective agar for the identification of MRSA. The MRSA identity of suspiciouslooking colonies was confirmed afterwards or excluded by another selective agar, chrom ID MRSA. All isolates from the selective agars with MRSA-specific colony morphology were identified by biochemical methods and mass spectrometry.Results: The initial CHROMagar MRSA screening identified suspicious colonies in 36 out of 1541 samples. A total of 25 of these 36 isolates showed MRSA-like growth on chrom ID agar. Out of these 25 isolates, 24 were confirmed as MRSA, while one isolate was identified as Staphylococcus kloosii. From the 11 strains that did not show suspicious growth on chrom ID agar, 3 were methicillin-sensitive Staphylococcus aureus(MSSA, with one instance of cocolonization with Corynebacterium spp.), 2 were confirmed as MRSA(with 1 instance of co-colonization with MSSA), 2 were lost during passaging and could not be re-cultured, one could not be identified by the applied approaches, and the remaining 3 strains were identified as Staphylococcus saprophyticus, Staphylococcus hominis(co-colonized with Macrococcus caseolyticus) and Staphylococcus cohnii, respectively.Conclusion: The application of the selective agar CHROMagar MRSA alone proved to be too non-specific to allow for a reliable diagnosis of the presence of MRSA. The combined use of two selective agars in a stepwise approach reduced this non-specificity with an acceptably low loss of sensitivity. Accordingly, such a stepwise screening approach might be an option for resource-restricted military medical field camps.
文摘中药首乌及其复方具有降血脂、抗动脉硬化、保护肝脏、抗菌、提高免疫功能、抗衰老等作用。近年来,对它的抗肿瘤作用颇有研究。但对其诱变性和抗诱变作用研究,尚未见报道。本实验以复方首乌片为样品,应用小鼠骨髓细胞染色体畸变试验(chrom-some aberration;CA)和骨髓多染红细胞微核试验(micronucleus of polychromaticerythrocyte;MNPCE)二项遗传指标,对该药进行诱变性和抗诱变性研究。
