Objective:To detect the CHRNA7 gene mutation and polymorphism in Southern Han Chinese patients with nocturnal frontal lobe epilepsy(NFLE).Methods:Blood samples were collected from 215 Southern Han Chinese patients wit...Objective:To detect the CHRNA7 gene mutation and polymorphism in Southern Han Chinese patients with nocturnal frontal lobe epilepsy(NFLE).Methods:Blood samples were collected from 215 Southern Han Chinese patients with NFLE and 200 healthy Southern Han Chinese control subjects.Genomic DNA was extracted,and CHRNA7 whole genome exons were amplified by the polymerase chain reaction and subjected to Sanger sequencing.Results:No CHRNA7 gene mutation was detected in all of the NFLE patients.However,five single nucleotide polymorphisms(SNPs)in sporadic cases were found,located in exons 5,6.and 7 of the CHRNA7 gene.Among them,c.690G>A and c.698A>G are known SNPs,while c.370G>A,c.654C>T,and c.497-498delTG were newly discovered SNPs.These SNPs were also found in some of the healthy controls.Conclusions:No CHRNA7 gene mutation was identified in Southern Han Chinese patients with NFLE.The CHRNA7 gene is probably not responsible for NFLE in this population.展开更多
Objective:To investigate the effect of Chinese herbal formula Ermiao Powder(二妙散,EMP)on the expression of cholinergic anti-inflammatory pathway in rats with rheumatoid arthritis(RA).Methods:Seventytwo rats were rand...Objective:To investigate the effect of Chinese herbal formula Ermiao Powder(二妙散,EMP)on the expression of cholinergic anti-inflammatory pathway in rats with rheumatoid arthritis(RA).Methods:Seventytwo rats were randomly divided into 6 groups according to body weight,including normal control group,collageninduced arthritis(CIA)group,three doses EMP groups,and methotrexate(MTX)group(n=12 per group).All of the rats except for those in the normal control group were given multipoint subcutaneous injection of bovine typeⅡcollagen to establish a CIA model.Three EMP groups received a high-(4.5 g/kg),medium-(3.0 g/kg),and low-(1.5 g/kg)doses of EMP by intragavage,respectively.MTX group was injected intraperitoneally MTX at 0.9 mg/kg once a week as the positive control.The administration was 3 consecutive weeks.Joint swelling,arthritis index,and body weight changes in different experimental groups of rats were tested.The joint damage was evaluated by masson staining.Quantitative real-time polymerase chain reaction,Western blot,and immunohistochemistry(IHC)were performed to evaluate the expression of CHRNA7,encodingα7 nicotinic acetylcholine receptor in the cholinergic anti-inflammatory pathway,in different tissues and their localization in the spleen and joints.Results:CHRNA7 expression levels were significantly higher in the joints and spleens of CIA group than those in normal control group(both P<0.05).Moreover,the CHRNA7 mRNA and protein levels in the spleen and joints of MTX and three doses of EMP groups were significantly lower than CIA group(all P<0.05).Compared with the MTX group,treatment with low-dose EMP resulted in significant reduction of CHRNA7 mRNA and protein expression levels(P<0.05 or P<0.01).IHC showed positive signals of CHRNA7 in the white pulp and red pulp of the spleens of rats;CHRNA7 was expressed on fibroblast-like synoviocytes,macrophages,and endothelial cells in the joints of rats,and the expression in the joints of low-dose EMP group was significantly lower than that in the CIA group(P<0.01).Conclusion:Cholinergic anti-inflammatory pathway was involved in the generation of the inflammatory reaction in CIA rats,and EMP exerted therapeutic effect on RA through cholinergic anti-inflammatory pathway.展开更多
基金supported by 2010 National Nature Science Foundation of China General Program(Program NO.8107I046)2012 Guangdong Provincial Science and Technology Program of China(Program NO.2012B032000009)2013 Guangdong Provincial Science and Technology Program of China(Program NO.2013B022000004)
文摘Objective:To detect the CHRNA7 gene mutation and polymorphism in Southern Han Chinese patients with nocturnal frontal lobe epilepsy(NFLE).Methods:Blood samples were collected from 215 Southern Han Chinese patients with NFLE and 200 healthy Southern Han Chinese control subjects.Genomic DNA was extracted,and CHRNA7 whole genome exons were amplified by the polymerase chain reaction and subjected to Sanger sequencing.Results:No CHRNA7 gene mutation was detected in all of the NFLE patients.However,five single nucleotide polymorphisms(SNPs)in sporadic cases were found,located in exons 5,6.and 7 of the CHRNA7 gene.Among them,c.690G>A and c.698A>G are known SNPs,while c.370G>A,c.654C>T,and c.497-498delTG were newly discovered SNPs.These SNPs were also found in some of the healthy controls.Conclusions:No CHRNA7 gene mutation was identified in Southern Han Chinese patients with NFLE.The CHRNA7 gene is probably not responsible for NFLE in this population.
基金Supported by the National Natural Science Foundation of China(No.81904034)the Basic Research Project of Shanxi Province(No.201801D121228)+1 种基金the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.201804001)the Research Project of Health Commission of Shanxi Province(No.2018001)。
文摘Objective:To investigate the effect of Chinese herbal formula Ermiao Powder(二妙散,EMP)on the expression of cholinergic anti-inflammatory pathway in rats with rheumatoid arthritis(RA).Methods:Seventytwo rats were randomly divided into 6 groups according to body weight,including normal control group,collageninduced arthritis(CIA)group,three doses EMP groups,and methotrexate(MTX)group(n=12 per group).All of the rats except for those in the normal control group were given multipoint subcutaneous injection of bovine typeⅡcollagen to establish a CIA model.Three EMP groups received a high-(4.5 g/kg),medium-(3.0 g/kg),and low-(1.5 g/kg)doses of EMP by intragavage,respectively.MTX group was injected intraperitoneally MTX at 0.9 mg/kg once a week as the positive control.The administration was 3 consecutive weeks.Joint swelling,arthritis index,and body weight changes in different experimental groups of rats were tested.The joint damage was evaluated by masson staining.Quantitative real-time polymerase chain reaction,Western blot,and immunohistochemistry(IHC)were performed to evaluate the expression of CHRNA7,encodingα7 nicotinic acetylcholine receptor in the cholinergic anti-inflammatory pathway,in different tissues and their localization in the spleen and joints.Results:CHRNA7 expression levels were significantly higher in the joints and spleens of CIA group than those in normal control group(both P<0.05).Moreover,the CHRNA7 mRNA and protein levels in the spleen and joints of MTX and three doses of EMP groups were significantly lower than CIA group(all P<0.05).Compared with the MTX group,treatment with low-dose EMP resulted in significant reduction of CHRNA7 mRNA and protein expression levels(P<0.05 or P<0.01).IHC showed positive signals of CHRNA7 in the white pulp and red pulp of the spleens of rats;CHRNA7 was expressed on fibroblast-like synoviocytes,macrophages,and endothelial cells in the joints of rats,and the expression in the joints of low-dose EMP group was significantly lower than that in the CIA group(P<0.01).Conclusion:Cholinergic anti-inflammatory pathway was involved in the generation of the inflammatory reaction in CIA rats,and EMP exerted therapeutic effect on RA through cholinergic anti-inflammatory pathway.
