研究了干酪乳杆菌Lactobacillus casei 393在小鼠肠道中定植能力及分布规律。利用羧基荧光素二乙酸琥珀酰亚胺酯分子探针对干酪乳杆菌Lactobacillus casei 393进行标记,以每只1010mL-1的量,口服途径喂给BALB/c鼠,分别于口服后的第1,3,5,...研究了干酪乳杆菌Lactobacillus casei 393在小鼠肠道中定植能力及分布规律。利用羧基荧光素二乙酸琥珀酰亚胺酯分子探针对干酪乳杆菌Lactobacillus casei 393进行标记,以每只1010mL-1的量,口服途径喂给BALB/c鼠,分别于口服后的第1,3,5,6 d取其十二指肠、空肠、回肠、结肠等肠段,通过流式细胞仪检测肠内的标记阳性菌。实验结果显示:经口服后第5 d,干酪乳杆菌Lactobacillus casei 393开始在BALB/c鼠肠道中定植,在十二指肠、空肠、回肠、结肠的定植率分别为28.70%,29.77%,15.63%,26.17%,第6 d标记的阳性菌出现明显的生长趋势,阳性检出率分别为71.77%,57.31%,65.44%,53.13%。研究表明干酪乳杆菌Lactobacillus casei 393具有良好的肠道定植能力。展开更多
目的应用荧光染料C FD A-SE(C arboxyfluorescein diacetate,succinim idyl ester)染色检测氧化苦参碱(O xym atrine,O M T)对刀豆蛋白A(C on A)刺激的小鼠淋巴细胞增殖的影响。方法利用C FD A-SE染色,流式细胞术检测小鼠T淋巴细胞在多...目的应用荧光染料C FD A-SE(C arboxyfluorescein diacetate,succinim idyl ester)染色检测氧化苦参碱(O xym atrine,O M T)对刀豆蛋白A(C on A)刺激的小鼠淋巴细胞增殖的影响。方法利用C FD A-SE染色,流式细胞术检测小鼠T淋巴细胞在多克隆刺激剂C on A和O M T的共同作同下荧光强度的变化,并应用相关软件分析O M T对小鼠淋巴细胞增殖的影响程度。结果培养48h,流式细胞仪检测的淋巴细胞C FSE荧光强度,对照组未出现子代峰,即淋巴细胞没有增殖。C on A组明显的出现3个峰,说明C on A刺激后48h细胞出现了明显的细胞增殖,与对照组相比荧光强度减半明显。在C on A+O M T各剂量组中,高剂量组即O M T500μg/m L组,原代峰显著高于C on A组,各子代峰显著低于C on A组,但与对照组比较差异无显著性,说明O M T明显抑制了淋巴细胞的增殖,中剂量组即O M T125μg/m L组和高剂量组结果一致,说明这一剂量的O M T仍然明显抑制小鼠淋巴细胞的增值,O M T低剂量组即O M T31μg/m L组和C on A组比较差异没有显著性,和对照组比较,各代细胞荧光强度差异有显著性,即这一剂量的O M T对淋巴细胞增殖抑制作用不明显。培养72h后,各组之间荧光强度变化情况与48h的情况基本一致。经C ELLQ uest软件拟合后得到各项增殖指标显示,O M T在500?展开更多
Objective To investigate the effects of low dose Oxymatrine(OMT)on mouse lymphocyte proliferation stimulated by Con A making use of fluorescence dyestuff CFDA-SE.Methods CFDA-SE staining and flow cytometry were used t...Objective To investigate the effects of low dose Oxymatrine(OMT)on mouse lymphocyte proliferation stimulated by Con A making use of fluorescence dyestuff CFDA-SE.Methods CFDA-SE staining and flow cytometry were used to detect the fluorescence intensity of lymphocytes after stimulated by polyclonal stimulators Con A and OMT.Then,related software was used to analyze the effects of OMT on mouse lymphocyte proliferation.Results After cultured for 48 h,CFSE fluorescence could be detected by cytometer,filial generation peaks did not appear in control group,which indicated that lymphocytes did not proliferate.Three peaks were obviously detected in Con A group which indicated that Lymphocytes divided after 48 h stimulated by Con A compared with the halving of the fluorescence intensity of control group.In groups with Con A and OMT treated,Primary generation peaks are all lower while filial generation peaks are significantly higher than groups with Con A treated only.This indicated OMT obviously promote lymphocyte proliferation.After cultured for 72 h,the fluorescence intensity changes between all groups are consistent with those of cultured for 48 h.Analyzed with CELLQuest software,it is shown that OMT could promote lymphocyte proliferation in 16,8,4 and 2μg/mL respectively.Conclusions 1)CFDA-SEdyeing and flow cytometer were both reliable tools to analyze lymphocyte proliferation;2)lower dosage of OMTcould promote the proliferation of lymphocyte as a immunopotentiator.展开更多
文摘研究了干酪乳杆菌Lactobacillus casei 393在小鼠肠道中定植能力及分布规律。利用羧基荧光素二乙酸琥珀酰亚胺酯分子探针对干酪乳杆菌Lactobacillus casei 393进行标记,以每只1010mL-1的量,口服途径喂给BALB/c鼠,分别于口服后的第1,3,5,6 d取其十二指肠、空肠、回肠、结肠等肠段,通过流式细胞仪检测肠内的标记阳性菌。实验结果显示:经口服后第5 d,干酪乳杆菌Lactobacillus casei 393开始在BALB/c鼠肠道中定植,在十二指肠、空肠、回肠、结肠的定植率分别为28.70%,29.77%,15.63%,26.17%,第6 d标记的阳性菌出现明显的生长趋势,阳性检出率分别为71.77%,57.31%,65.44%,53.13%。研究表明干酪乳杆菌Lactobacillus casei 393具有良好的肠道定植能力。
文摘目的应用荧光染料C FD A-SE(C arboxyfluorescein diacetate,succinim idyl ester)染色检测氧化苦参碱(O xym atrine,O M T)对刀豆蛋白A(C on A)刺激的小鼠淋巴细胞增殖的影响。方法利用C FD A-SE染色,流式细胞术检测小鼠T淋巴细胞在多克隆刺激剂C on A和O M T的共同作同下荧光强度的变化,并应用相关软件分析O M T对小鼠淋巴细胞增殖的影响程度。结果培养48h,流式细胞仪检测的淋巴细胞C FSE荧光强度,对照组未出现子代峰,即淋巴细胞没有增殖。C on A组明显的出现3个峰,说明C on A刺激后48h细胞出现了明显的细胞增殖,与对照组相比荧光强度减半明显。在C on A+O M T各剂量组中,高剂量组即O M T500μg/m L组,原代峰显著高于C on A组,各子代峰显著低于C on A组,但与对照组比较差异无显著性,说明O M T明显抑制了淋巴细胞的增殖,中剂量组即O M T125μg/m L组和高剂量组结果一致,说明这一剂量的O M T仍然明显抑制小鼠淋巴细胞的增值,O M T低剂量组即O M T31μg/m L组和C on A组比较差异没有显著性,和对照组比较,各代细胞荧光强度差异有显著性,即这一剂量的O M T对淋巴细胞增殖抑制作用不明显。培养72h后,各组之间荧光强度变化情况与48h的情况基本一致。经C ELLQ uest软件拟合后得到各项增殖指标显示,O M T在500?
文摘Objective To investigate the effects of low dose Oxymatrine(OMT)on mouse lymphocyte proliferation stimulated by Con A making use of fluorescence dyestuff CFDA-SE.Methods CFDA-SE staining and flow cytometry were used to detect the fluorescence intensity of lymphocytes after stimulated by polyclonal stimulators Con A and OMT.Then,related software was used to analyze the effects of OMT on mouse lymphocyte proliferation.Results After cultured for 48 h,CFSE fluorescence could be detected by cytometer,filial generation peaks did not appear in control group,which indicated that lymphocytes did not proliferate.Three peaks were obviously detected in Con A group which indicated that Lymphocytes divided after 48 h stimulated by Con A compared with the halving of the fluorescence intensity of control group.In groups with Con A and OMT treated,Primary generation peaks are all lower while filial generation peaks are significantly higher than groups with Con A treated only.This indicated OMT obviously promote lymphocyte proliferation.After cultured for 72 h,the fluorescence intensity changes between all groups are consistent with those of cultured for 48 h.Analyzed with CELLQuest software,it is shown that OMT could promote lymphocyte proliferation in 16,8,4 and 2μg/mL respectively.Conclusions 1)CFDA-SEdyeing and flow cytometer were both reliable tools to analyze lymphocyte proliferation;2)lower dosage of OMTcould promote the proliferation of lymphocyte as a immunopotentiator.
