Fresh semen has a very limited life span after it leaves the gonad. The relative research shows that the epididymal plasma provides a special environment to spermatozoa for survival. In this study, the osmolarity and ...Fresh semen has a very limited life span after it leaves the gonad. The relative research shows that the epididymal plasma provides a special environment to spermatozoa for survival. In this study, the osmolarity and protein concentration of caput and caudal bull epididymis were analyzed. Bovine caudal epididymal sperm were incubated in different protein and sorbitol combinations of CEP-2 diluents at 4 ℃ for 120 h and sperm viability was assessed. Under this condition, sperm viability was greater than 60% after 5 d. The osmolarity of caput and caudal epididymis were （287.0 ± 13.7） and （310.8 ± 17.0 ） mOsm, respectively. The protein concentration of caput and caudal epididymis were （ 37.43 ± 12.55 ） and （50.58±11.08） mg/mg, respectively. This may have an application in the animal production industry.展开更多
文摘Fresh semen has a very limited life span after it leaves the gonad. The relative research shows that the epididymal plasma provides a special environment to spermatozoa for survival. In this study, the osmolarity and protein concentration of caput and caudal bull epididymis were analyzed. Bovine caudal epididymal sperm were incubated in different protein and sorbitol combinations of CEP-2 diluents at 4 ℃ for 120 h and sperm viability was assessed. Under this condition, sperm viability was greater than 60% after 5 d. The osmolarity of caput and caudal epididymis were （287.0 ± 13.7） and （310.8 ± 17.0 ） mOsm, respectively. The protein concentration of caput and caudal epididymis were （ 37.43 ± 12.55 ） and （50.58±11.08） mg/mg, respectively. This may have an application in the animal production industry.
