Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （SA） and the correlation study of CENP-C expression with chromosome segregation...Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （SA） and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization （FISH） and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group （n=30） and control group （n--30） according to the results with FISH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight （51.06%） cases had observed the numerical chromosome abnormality, including 30positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group （P〈0.05）. Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid.展开更多
Recently the antichromosome antisera from several scleroderma patients have been found to recognize the pellicle of metaphase and anaphase chromosomes. In order to identify the pellicle components, we used these antic...Recently the antichromosome antisera from several scleroderma patients have been found to recognize the pellicle of metaphase and anaphase chromosomes. In order to identify the pellicle components, we used these antichromosome antisera to screen a human embryonic cDNA library. The sequences of the positive clones are identical to the cDNA gene sequence of CENP-C (centromere protein C), a human centromere autoantigen. This result suggusts that CENP-C is a component of the pellicle of human metaphase and anaphase chromosomes.展开更多
基金supported by the Natural Science Foundation of Shanxi province[2012021035-1]
文摘Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （SA） and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization （FISH） and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group （n=30） and control group （n--30） according to the results with FISH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight （51.06%） cases had observed the numerical chromosome abnormality, including 30positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group （P〈0.05）. Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid.
文摘Recently the antichromosome antisera from several scleroderma patients have been found to recognize the pellicle of metaphase and anaphase chromosomes. In order to identify the pellicle components, we used these antichromosome antisera to screen a human embryonic cDNA library. The sequences of the positive clones are identical to the cDNA gene sequence of CENP-C (centromere protein C), a human centromere autoantigen. This result suggusts that CENP-C is a component of the pellicle of human metaphase and anaphase chromosomes.
