The development of sustainable sludge management systems requires looking at them with a new vision in which the concepts of SD(Sustainable Development)must integrate those of CE(Circular Economy),both concepts subjec...The development of sustainable sludge management systems requires looking at them with a new vision in which the concepts of SD(Sustainable Development)must integrate those of CE(Circular Economy),both concepts subject to the principles of TD(Thermodynamics),thus allowing the adoption of actions that are all the more effective the more complete the evaluation of the social dimension has been.This involves a new“Way of thinking”which sees the sludge system as the“Locomotive”of the entire wastewater/sludge treatment train and is developed through“Ways of acting”which includes both“Technical”actions to maximize recoveries of useful materials and/or or energy,and“Socio/Institutional”actions to overcome barriers linked to local cultures and traditions,also considering that the specific local context heavily influences the choices capable of satisfying the concepts of CE.It follows the need of issuing realistic and applicable regulations and overcoming social barriers,such as lack of infrastructure and/or qualified personnel,to achieve an effective integration of the concepts of CE with the more general ones of sustainability.展开更多
Active matter exhibits collective motions at various scales.Geometric confinement has been identified as an effective way to control and manipulate active fluids,with much attention given to external factors.However,t...Active matter exhibits collective motions at various scales.Geometric confinement has been identified as an effective way to control and manipulate active fluids,with much attention given to external factors.However,the impact of the inherent properties of active particles on collective motion under confined conditions remains elusive.Here,we use a highly tunable active nematics model to study active systems under confinement,focusing on the effect of the self-driven speed of active particles.We identify three distinct states characterized by unique particle and flow fields within confined active nematic systems,among which circular rotation emerges as a collective motion involving rotational movement in both particle and flow fields.The theoretical phase diagram shows that increasing the self-driven speed of active particles significantly enhances the region of the circular rotation state and improves its stability.Our results provide insights into the formation of high quality vortices in confined active nematic systems.展开更多
Circular RNAs(circRNAs)are a distinct type of nonlinear and noncoding RNAs endogenously expressed by pre-mRNA back-splicing and crucial in transcriptional and posttranscriptional regulation.CircRNAs can regulate cellu...Circular RNAs(circRNAs)are a distinct type of nonlinear and noncoding RNAs endogenously expressed by pre-mRNA back-splicing and crucial in transcriptional and posttranscriptional regulation.CircRNAs can regulate cellular and molecular pathways through various mechanisms,such as microRNA sponging.Numerous studies have indicated the regulatory roles of circRNAs in the osteogenic differentiation of stem cells(SCs)isolated from different sources.Dental tissue-derived mesenchymal SCs(MSCs)have received considerable attention in artificial bone engineering,in which SCs are used to manufacture functional bone tissues to repair bone defects.Recently,studies have reported the regulatory roles of circRNAs in the osteogenic differentiation of dental-derived MSCs,such as apical papillae,dental pulp,and dental follicle SCs.This review aimed to discuss the findings of studies evaluating the contribution of circRNAs to the osteogenic differentiation of dental-derived MSCs.展开更多
Owing to eccentricity and inclination, circularity of a cylindrical workpiece cannot be measured precisely by a circularity measuring machine when the workpiece has a small dimension(diameter ≤ 3 mm). In this paper, ...Owing to eccentricity and inclination, circularity of a cylindrical workpiece cannot be measured precisely by a circularity measuring machine when the workpiece has a small dimension(diameter ≤ 3 mm). In this paper, with the aim of solving this problem, circularity metrology of a small cylindrical workpiece using a segmenting scanning method is analyzed. The cross-sectional circle of the cylinder is segmented into several equivalent arcs for measurement by a two-dimensional coordinate measuring machine(profilometer). The circularity contour is obtained by stitching together arc contours obtained by data processing of the coordinates. Different segmenting patterns for coordinate scanning are considered. Measurement results are presented for three segmentation patterns, with 8, 10, and 12 equal segments, respectively.These results are evaluated in terms of the matching coefficient between neighboring arc contours on circumferential stitching, the Euclidean distance between neighboring arc contours on radial stitching, and the curvature of the arcs. From these evaluations, it is found that as the number of segments is increased, the matching coefficient increases from 0.14 to 0.50, the Euclidean distance decreases from 32 nm to 26 nm,and the curvature becomes close to the standard value.展开更多
BACKGROUND Myocardial ischemia/reperfusion(I/R)injury,which is associated with high morbidity and mortality,is a main cause of unexpected myocardial injury after acute myocardial infarction.However,the underlying mech...BACKGROUND Myocardial ischemia/reperfusion(I/R)injury,which is associated with high morbidity and mortality,is a main cause of unexpected myocardial injury after acute myocardial infarction.However,the underlying mechanism remains unclear.Circular RNAs(circRNAs),which are formed from protein-coding genes,can sequester microRNAs or proteins,modulate transcription and interfere with splicing.Authoritative studies suggest that circRNAs may play an important role in myocardial I/R injury.AIM To explore the role and mechanism of circRNAs in myocardial I/R injury.METHODS We constructed a myocardial I/R injury model using ligation of the left anterior descending coronary artery,and evaluated the success of the validated model using triphenyltetrazolium chloride and hematoxylin-eosin staining.Then,left ventricular samples from different groups were selected for mRNA-sequence,and differential gene screening was performed on the obtained results.The differentially obtained mRNAs were divided into up-regulated and down-regulated according to their expression levels,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional enrichment analysis were performed,respectively.Then,the obtained circRNA and microRNA(miRNA)were paired for analysis,and the binding sites of miRNA and mRNA were virtual screened.Finally,the obtained circRNA,miRNA and mRNA were constructed by ceRNA mutual most useful network.RESULTS We used an RNA sequencing array to investigate the expression signatures of circRNAs in myocardial I/R injury using three samples from the I/R group and three samples from the sham group.A total of 142 upregulated and 121 downregulated circRNAs were found to be differentially expressed(fold change≥2,P<0.05).GO and KEGG functional analyses of these circRNAs were performed.GO analysis revealed that these circRNAs were involved mainly in cellular and intracellular processes.KEGG analysis demonstrated that 6 of the top 20 pathways were correlated with cell apoptosis.Furthermore,a circRNA-miRNA coexpression network and ceRNA network based on these genes were constructed,revealing that mmu-circ-0001452,mmu-circ-0001637,and mmu-circ-0000870 might be key regulators of myocardial I/R injury.CONCLUSION This research provides new insights into the mechanism of myocardial I/R,which mmu-circ-0001452,mmu-circ-0001637,and mmu-circ-0000870 are expected to be new therapeutic targets for myocardial I/R injury.展开更多
With the increasing complexity of hotel selection,traditional decision-making models often struggle to account for uncertainty and interrelated criteria.Multi-criteria decision-making(MCDM)techniques,particularly thos...With the increasing complexity of hotel selection,traditional decision-making models often struggle to account for uncertainty and interrelated criteria.Multi-criteria decision-making(MCDM)techniques,particularly those based on fuzzy logic,provide a robust framework for handling such challenges.This paper presents a novel approach to MCDM within the framework of Circular Intuitionistic Fuzzy Sets(C-IFS)by combining three distinct methodologies:Weighted Aggregated Sum Product Assessment(WASPAS),an Alternative Ranking Order Method Accounting for Two-Step Normalization(AROMAN),and the CRITIC method(Criteria Importance Through Inter-criteria Correlation).To address the dynamic nature of traveler preferences in hotel selection,the study employs a comprehensive set of criteria encompassing aspects such as location proximity,amenities,pricing,customer reviews,environmental impact,safety,booking flexibility,and cultural experiences.The CRITIC method is used to determine the importance of each criterion by assessing intercriteria correlations.AROMAN is employed for the systematic evaluation of alternatives,considering their additive relationships and providing a weighted assessment.WASPAS further analyzes the results obtained from AROMAN,incorporating both positive and negative aspects for a comprehensive evaluation.The integration of C-IFS enhances the model’s ability to manage uncertainty and imprecision in the decision-making process.Through a case study,we demonstrate the effectiveness of this integrated approach,offering decision-makers valuable insights for selecting the most suitable hotel option in alignment with the diverse preferences of contemporary travelers.This research contributes to the evolving field of decision science by showcasing the practical applicability of these methodologies within a C-IFS framework for complex decision scenarios.展开更多
Colorectal cancer(CRC)is a common and deadly malignancy worldwide,causing high morbidity and mortality rates.Chemoresistance continues to be the major barrier to the effective treatment of CRC.Circular RNA(circRNA),a ...Colorectal cancer(CRC)is a common and deadly malignancy worldwide,causing high morbidity and mortality rates.Chemoresistance continues to be the major barrier to the effective treatment of CRC.Circular RNA(circRNA),a recently identified class of non-coding RNA,has become an emerging focus in CRC research due to its abundant presence and stability in extracellular vesicles(EVs).The circRNA of EVs(EVs-circRNA)is closely associated with CRC progression and plays a pivotal role in chemoresistance mechanisms.This paper investigates the circRNA mechanism underlying different chemoresistance scenarios in CRC and explores the dual role of EVs-circRNA in mediating chemotherapy resistance.Furthermore,EVs-circRNA,a non-invasive biomarker for liquid biopsies,holds significant promise in clinical applications,ranging from early CRC diagnosis to monitoring disease progression and assessing prognosis.These studies provide a new perspective for uncovering CRC pathogenesis and lay the foundation for precision therapy and personalized treatment strategies for CRC.In the future,therapeutic strategies targeting EVs-circRNAs are anticipated to revolutionize CRC treatment,leading to improved therapeutic outcomes and enhanced quality of life for patients.展开更多
In this paper,a type of reinforcing structure for composite shell with single and through hole is presented.The experimental tests for the composite shells without hole,with single hole and reinforced structure,with t...In this paper,a type of reinforcing structure for composite shell with single and through hole is presented.The experimental tests for the composite shells without hole,with single hole and reinforced structure,with through hole and reinforced structure subjected to hydrostatic pressure were carried out by the designed experimental test system.The mechanical responses of the composite shells under hydrostatic pressure are obtained by the high-speed camera and strain measurement.The results show that the entire deformation process of the shell can be divided into three:uniform compression,"buckling mode formation"and buckling.The"buckling mode formation"process is captured and reported for the first time.For the composite shell with single hole,the proposed reinforcing structure has a significant reinforcement effect on the shell and the buckling capacity of the shell is not weaker than the complete composite shell.For the composite shell with through hole,sealing effect can be achieved by the proposed reinforcing structure,but the buckling capacity of the shell after reinforcement can only reach 77%of the original buckling capacity.展开更多
BACKGROUND Extramural venous invasion(EMVI)is a critical prognostic factor in gastric cancer(GC);however,its detection and underlying molecular mechanisms remain underexplored.AIM To investigate the relationship betwe...BACKGROUND Extramural venous invasion(EMVI)is a critical prognostic factor in gastric cancer(GC);however,its detection and underlying molecular mechanisms remain underexplored.AIM To investigate the relationship between EMVI and expression of the circular RNA hsa_circ_0097977 in orthotopic GC mouse models.METHODS A retrospective analysis was conducted in addition to a preclinical animal study,involving 13 GC patients and 24 orthotopic GC mouse models,respectively.EMVI was assessed using axial T2-weighted fat suppression sequences on a 9.4T magnetic resonance imaging(MRI)with histopathological confirmation as the gold standard for EMVI.The impact of hsa_circ_0097977 on EMVI and GC cell function was evaluated.Statistical analyses comprised consistency,area under the curve analysis,correlation,χ^(2)/Fisher exact,and Mann-Whitney U/t-tests,with significance set at P<0.05.RESULTS EMVI was accurately detected using 9.4T MRI in orthotopic mouse models with an area under the curve of 0.843(sensitivity 78.6%,specificity 90.0%).MRI detected EMVI was the only imaging factor associated with distant metastasis(P=0.04).Furthermore,knockdown of hsa_circ_0097977 was the only factor associated with EMVI(P=0.043,0.038)and led to reduced invasion and increased apoptosis in GC cells.CONCLUSION EMVI,a risk factor for distant metastasis in GC,is detectable by 9.4T MRI and regulated by hsa_circ_0097977,making it a potential therapeutic target.展开更多
BACKGROUND As a non-coding RNA molecule,circular RNAs(circRNAs)have significant specificity,and existing data suggest a close relationship between them and the prognosis of patients with gastric cancer(GC).However,thi...BACKGROUND As a non-coding RNA molecule,circular RNAs(circRNAs)have significant specificity,and existing data suggest a close relationship between them and the prognosis of patients with gastric cancer(GC).However,this mechanism has no evidence yet.This article explores the functions of hsa_circRNA_102415 in the malignant behavior and potential downstream signaling of GC cells.The chosen approach is loss of signal and functional gain.AIM To investigate and analyze the relationship between hsa_circRNA_102415 and GC and explore its specific role.Results provide reference for other researchers to develop targeted treatment plans.METHODS The gene expression omnibus(GEO)database can be used to obtain the microarray dataset GSE83521.Data were analyzed using the GEO2R tool to identify differences in circRNAs between normal and GC samples.Quantitative real-time polymerase chain reaction was used to detect differentially expressed genes in GC tissue samples and adjacent cancer tissue samples.GC cells were transfected with small interfering-hsa_circRNA_104415 and plasmid DNA(pcDNA)-hsa_ircRNA_102415.Multiple detection methods,such as Transwell and cell counting kit 8,were used to evaluate cellular physiological activities,including cell invasion and proliferation.The relationship between Wnt family members 2B,microRNA(miR)-4529-5p,etc.,including argonaute 2-RNA immunoprecipitation and luciferase reporter genes was analyzed.Rescue experiments were conducted to analyze and explore the relationship between the malignant behavior of GC cells and hsa_circRNA_102415.RESULTS GEO2R analysis confirmed that hsa_circRNA_102415 had significantly higher expression levels in disease tissues.hsa_circRNA_102415 and miR-4529-5p showed a negative correlation in disease cells,suggesting that hsa_circRNA_102415 upregulated WNT2B expression in GC cells as a competing endogenous RNA for miR-4529-5p.miR-4529-5p mimic or small interfering-WNT2B reversed the effects of pcDNA-hsa_circRNA_102415 or miR-4529-5p inhibitor on cell malignant functions.CONCLUSION miR-4529-5p was used to successfully activate the potential of WNT2B,clarify the role of hsa_circRNA_102415 in GC cells,and provide reference for other researchers to develop targeted treatment plans.展开更多
Background:Sunitinib resistance is a major challenge in advanced renal cell carcinoma(RCC).Clinically,elucidating the underlying mechanisms and developing practical countermeasures for sunitinib resistance in RCC is d...Background:Sunitinib resistance is a major challenge in advanced renal cell carcinoma(RCC).Clinically,elucidating the underlying mechanisms and developing practical countermeasures for sunitinib resistance in RCC is desirable.In previous studies,we found that circAGAP1 expression was significantly upregulated in clear cell RCC(ccRCC)and was strongly associated with poor prognosis.However,the role of circAGAP1 in sunitinib resistance in ccRCC remains unclear.Methods:We used public databases for bioinformatics analysis to identify the binding targets of circAGAP1.Additionally,the effects of circAGAP1 on the proliferation,clonogenesis,apoptosis,and migration of ccRCC cells were analyzed using quantitative real-time PCR,cell counting kit-8 assays,migration and apoptosis assays,and colony formation assays.Furthermore,RNA immunoprecipitation,dual-luciferase reporter,and fluorescence in situ hybridization assays were used to explore the molecular mechanism.Results:In this study,circAGAP1 exhibited higher expression in sunitinib-sensitive ccRCC cells and inhibited the clonogenesis,proliferation,and migration of ccRCC cells after sunitinib treatment.Mechanical studies revealed that circAGAP1 regulated the expression of sunitinib target platelet-derived growth factor receptor by acting as a microRNA sponge that suppresses miR-149-5p,miR-455-5p,and miR-15a-5p simultaneously.Overexpression of these three miRNAs reversed circAGAP1-mediated sunitinib sensitivity in ccRCC.Conclusions:In summary,our findings indicate that circAGAP1 may serve as a promising biomarker to predict sunitinib sensibility and a therapeutic target in ccRCC.展开更多
文摘The development of sustainable sludge management systems requires looking at them with a new vision in which the concepts of SD(Sustainable Development)must integrate those of CE(Circular Economy),both concepts subject to the principles of TD(Thermodynamics),thus allowing the adoption of actions that are all the more effective the more complete the evaluation of the social dimension has been.This involves a new“Way of thinking”which sees the sludge system as the“Locomotive”of the entire wastewater/sludge treatment train and is developed through“Ways of acting”which includes both“Technical”actions to maximize recoveries of useful materials and/or or energy,and“Socio/Institutional”actions to overcome barriers linked to local cultures and traditions,also considering that the specific local context heavily influences the choices capable of satisfying the concepts of CE.It follows the need of issuing realistic and applicable regulations and overcoming social barriers,such as lack of infrastructure and/or qualified personnel,to achieve an effective integration of the concepts of CE with the more general ones of sustainability.
基金supported by the National Key Research and Development Program of China under Grant No.2022YFA1405000Innovation Program for Quantum Science and Technology under Grant No.2024ZD0300101the National Natural Science Foundation of China under Grant Nos.12274212,12174184,12347102。
文摘Active matter exhibits collective motions at various scales.Geometric confinement has been identified as an effective way to control and manipulate active fluids,with much attention given to external factors.However,the impact of the inherent properties of active particles on collective motion under confined conditions remains elusive.Here,we use a highly tunable active nematics model to study active systems under confinement,focusing on the effect of the self-driven speed of active particles.We identify three distinct states characterized by unique particle and flow fields within confined active nematic systems,among which circular rotation emerges as a collective motion involving rotational movement in both particle and flow fields.The theoretical phase diagram shows that increasing the self-driven speed of active particles significantly enhances the region of the circular rotation state and improves its stability.Our results provide insights into the formation of high quality vortices in confined active nematic systems.
文摘Circular RNAs(circRNAs)are a distinct type of nonlinear and noncoding RNAs endogenously expressed by pre-mRNA back-splicing and crucial in transcriptional and posttranscriptional regulation.CircRNAs can regulate cellular and molecular pathways through various mechanisms,such as microRNA sponging.Numerous studies have indicated the regulatory roles of circRNAs in the osteogenic differentiation of stem cells(SCs)isolated from different sources.Dental tissue-derived mesenchymal SCs(MSCs)have received considerable attention in artificial bone engineering,in which SCs are used to manufacture functional bone tissues to repair bone defects.Recently,studies have reported the regulatory roles of circRNAs in the osteogenic differentiation of dental-derived MSCs,such as apical papillae,dental pulp,and dental follicle SCs.This review aimed to discuss the findings of studies evaluating the contribution of circRNAs to the osteogenic differentiation of dental-derived MSCs.
基金supported by the National Defense Basic Scientific Research Program of China(Grant No.JCKY2019427D002).
文摘Owing to eccentricity and inclination, circularity of a cylindrical workpiece cannot be measured precisely by a circularity measuring machine when the workpiece has a small dimension(diameter ≤ 3 mm). In this paper, with the aim of solving this problem, circularity metrology of a small cylindrical workpiece using a segmenting scanning method is analyzed. The cross-sectional circle of the cylinder is segmented into several equivalent arcs for measurement by a two-dimensional coordinate measuring machine(profilometer). The circularity contour is obtained by stitching together arc contours obtained by data processing of the coordinates. Different segmenting patterns for coordinate scanning are considered. Measurement results are presented for three segmentation patterns, with 8, 10, and 12 equal segments, respectively.These results are evaluated in terms of the matching coefficient between neighboring arc contours on circumferential stitching, the Euclidean distance between neighboring arc contours on radial stitching, and the curvature of the arcs. From these evaluations, it is found that as the number of segments is increased, the matching coefficient increases from 0.14 to 0.50, the Euclidean distance decreases from 32 nm to 26 nm,and the curvature becomes close to the standard value.
基金Supported by Zhejiang Provincial Natural Science Foundation of China,No.LQ23H020004The Medical and Health Research Project of Zhejiang province,No.2024KY983Basic Medical Health Technology Project of Wenzhou Science and Technology Bureau,No.Y20210818 and No.Y20210140.
文摘BACKGROUND Myocardial ischemia/reperfusion(I/R)injury,which is associated with high morbidity and mortality,is a main cause of unexpected myocardial injury after acute myocardial infarction.However,the underlying mechanism remains unclear.Circular RNAs(circRNAs),which are formed from protein-coding genes,can sequester microRNAs or proteins,modulate transcription and interfere with splicing.Authoritative studies suggest that circRNAs may play an important role in myocardial I/R injury.AIM To explore the role and mechanism of circRNAs in myocardial I/R injury.METHODS We constructed a myocardial I/R injury model using ligation of the left anterior descending coronary artery,and evaluated the success of the validated model using triphenyltetrazolium chloride and hematoxylin-eosin staining.Then,left ventricular samples from different groups were selected for mRNA-sequence,and differential gene screening was performed on the obtained results.The differentially obtained mRNAs were divided into up-regulated and down-regulated according to their expression levels,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional enrichment analysis were performed,respectively.Then,the obtained circRNA and microRNA(miRNA)were paired for analysis,and the binding sites of miRNA and mRNA were virtual screened.Finally,the obtained circRNA,miRNA and mRNA were constructed by ceRNA mutual most useful network.RESULTS We used an RNA sequencing array to investigate the expression signatures of circRNAs in myocardial I/R injury using three samples from the I/R group and three samples from the sham group.A total of 142 upregulated and 121 downregulated circRNAs were found to be differentially expressed(fold change≥2,P<0.05).GO and KEGG functional analyses of these circRNAs were performed.GO analysis revealed that these circRNAs were involved mainly in cellular and intracellular processes.KEGG analysis demonstrated that 6 of the top 20 pathways were correlated with cell apoptosis.Furthermore,a circRNA-miRNA coexpression network and ceRNA network based on these genes were constructed,revealing that mmu-circ-0001452,mmu-circ-0001637,and mmu-circ-0000870 might be key regulators of myocardial I/R injury.CONCLUSION This research provides new insights into the mechanism of myocardial I/R,which mmu-circ-0001452,mmu-circ-0001637,and mmu-circ-0000870 are expected to be new therapeutic targets for myocardial I/R injury.
基金supported by the Princess Nourah bint Abdulrahman University Researchers Supporting Project Number(PNURSP2025R259)Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia+1 种基金supported by the Researchers Supporting Project Number(UM-DSR-IG-2023-07)Almaarefa University,Riyadh,Saudi Arabia.
文摘With the increasing complexity of hotel selection,traditional decision-making models often struggle to account for uncertainty and interrelated criteria.Multi-criteria decision-making(MCDM)techniques,particularly those based on fuzzy logic,provide a robust framework for handling such challenges.This paper presents a novel approach to MCDM within the framework of Circular Intuitionistic Fuzzy Sets(C-IFS)by combining three distinct methodologies:Weighted Aggregated Sum Product Assessment(WASPAS),an Alternative Ranking Order Method Accounting for Two-Step Normalization(AROMAN),and the CRITIC method(Criteria Importance Through Inter-criteria Correlation).To address the dynamic nature of traveler preferences in hotel selection,the study employs a comprehensive set of criteria encompassing aspects such as location proximity,amenities,pricing,customer reviews,environmental impact,safety,booking flexibility,and cultural experiences.The CRITIC method is used to determine the importance of each criterion by assessing intercriteria correlations.AROMAN is employed for the systematic evaluation of alternatives,considering their additive relationships and providing a weighted assessment.WASPAS further analyzes the results obtained from AROMAN,incorporating both positive and negative aspects for a comprehensive evaluation.The integration of C-IFS enhances the model’s ability to manage uncertainty and imprecision in the decision-making process.Through a case study,we demonstrate the effectiveness of this integrated approach,offering decision-makers valuable insights for selecting the most suitable hotel option in alignment with the diverse preferences of contemporary travelers.This research contributes to the evolving field of decision science by showcasing the practical applicability of these methodologies within a C-IFS framework for complex decision scenarios.
基金Supported by Open Project of National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,No.NCRCOP2023007.
文摘Colorectal cancer(CRC)is a common and deadly malignancy worldwide,causing high morbidity and mortality rates.Chemoresistance continues to be the major barrier to the effective treatment of CRC.Circular RNA(circRNA),a recently identified class of non-coding RNA,has become an emerging focus in CRC research due to its abundant presence and stability in extracellular vesicles(EVs).The circRNA of EVs(EVs-circRNA)is closely associated with CRC progression and plays a pivotal role in chemoresistance mechanisms.This paper investigates the circRNA mechanism underlying different chemoresistance scenarios in CRC and explores the dual role of EVs-circRNA in mediating chemotherapy resistance.Furthermore,EVs-circRNA,a non-invasive biomarker for liquid biopsies,holds significant promise in clinical applications,ranging from early CRC diagnosis to monitoring disease progression and assessing prognosis.These studies provide a new perspective for uncovering CRC pathogenesis and lay the foundation for precision therapy and personalized treatment strategies for CRC.In the future,therapeutic strategies targeting EVs-circRNAs are anticipated to revolutionize CRC treatment,leading to improved therapeutic outcomes and enhanced quality of life for patients.
基金supported by the Ningbo Major Research and Development Plan Project(Grant No.2024Z135)the Natural Science Basic Research Program of Shaanxi Province(Grant No.2024JC-YBMS-322)+1 种基金China Postdoctoral Science Foundation(Grant No.2020M673492)National Natural Science Foundation of China(Grant No.51909219)。
文摘In this paper,a type of reinforcing structure for composite shell with single and through hole is presented.The experimental tests for the composite shells without hole,with single hole and reinforced structure,with through hole and reinforced structure subjected to hydrostatic pressure were carried out by the designed experimental test system.The mechanical responses of the composite shells under hydrostatic pressure are obtained by the high-speed camera and strain measurement.The results show that the entire deformation process of the shell can be divided into three:uniform compression,"buckling mode formation"and buckling.The"buckling mode formation"process is captured and reported for the first time.For the composite shell with single hole,the proposed reinforcing structure has a significant reinforcement effect on the shell and the buckling capacity of the shell is not weaker than the complete composite shell.For the composite shell with through hole,sealing effect can be achieved by the proposed reinforcing structure,but the buckling capacity of the shell after reinforcement can only reach 77%of the original buckling capacity.
基金Supported by The National Natural Science Foundation of China,No.81901819Peking University People’s Hospital Scientific Research Development Funds,No.RDX2024-08Beijing Natural Science Foundation,No.7232187.
文摘BACKGROUND Extramural venous invasion(EMVI)is a critical prognostic factor in gastric cancer(GC);however,its detection and underlying molecular mechanisms remain underexplored.AIM To investigate the relationship between EMVI and expression of the circular RNA hsa_circ_0097977 in orthotopic GC mouse models.METHODS A retrospective analysis was conducted in addition to a preclinical animal study,involving 13 GC patients and 24 orthotopic GC mouse models,respectively.EMVI was assessed using axial T2-weighted fat suppression sequences on a 9.4T magnetic resonance imaging(MRI)with histopathological confirmation as the gold standard for EMVI.The impact of hsa_circ_0097977 on EMVI and GC cell function was evaluated.Statistical analyses comprised consistency,area under the curve analysis,correlation,χ^(2)/Fisher exact,and Mann-Whitney U/t-tests,with significance set at P<0.05.RESULTS EMVI was accurately detected using 9.4T MRI in orthotopic mouse models with an area under the curve of 0.843(sensitivity 78.6%,specificity 90.0%).MRI detected EMVI was the only imaging factor associated with distant metastasis(P=0.04).Furthermore,knockdown of hsa_circ_0097977 was the only factor associated with EMVI(P=0.043,0.038)and led to reduced invasion and increased apoptosis in GC cells.CONCLUSION EMVI,a risk factor for distant metastasis in GC,is detectable by 9.4T MRI and regulated by hsa_circ_0097977,making it a potential therapeutic target.
文摘BACKGROUND As a non-coding RNA molecule,circular RNAs(circRNAs)have significant specificity,and existing data suggest a close relationship between them and the prognosis of patients with gastric cancer(GC).However,this mechanism has no evidence yet.This article explores the functions of hsa_circRNA_102415 in the malignant behavior and potential downstream signaling of GC cells.The chosen approach is loss of signal and functional gain.AIM To investigate and analyze the relationship between hsa_circRNA_102415 and GC and explore its specific role.Results provide reference for other researchers to develop targeted treatment plans.METHODS The gene expression omnibus(GEO)database can be used to obtain the microarray dataset GSE83521.Data were analyzed using the GEO2R tool to identify differences in circRNAs between normal and GC samples.Quantitative real-time polymerase chain reaction was used to detect differentially expressed genes in GC tissue samples and adjacent cancer tissue samples.GC cells were transfected with small interfering-hsa_circRNA_104415 and plasmid DNA(pcDNA)-hsa_ircRNA_102415.Multiple detection methods,such as Transwell and cell counting kit 8,were used to evaluate cellular physiological activities,including cell invasion and proliferation.The relationship between Wnt family members 2B,microRNA(miR)-4529-5p,etc.,including argonaute 2-RNA immunoprecipitation and luciferase reporter genes was analyzed.Rescue experiments were conducted to analyze and explore the relationship between the malignant behavior of GC cells and hsa_circRNA_102415.RESULTS GEO2R analysis confirmed that hsa_circRNA_102415 had significantly higher expression levels in disease tissues.hsa_circRNA_102415 and miR-4529-5p showed a negative correlation in disease cells,suggesting that hsa_circRNA_102415 upregulated WNT2B expression in GC cells as a competing endogenous RNA for miR-4529-5p.miR-4529-5p mimic or small interfering-WNT2B reversed the effects of pcDNA-hsa_circRNA_102415 or miR-4529-5p inhibitor on cell malignant functions.CONCLUSION miR-4529-5p was used to successfully activate the potential of WNT2B,clarify the role of hsa_circRNA_102415 in GC cells,and provide reference for other researchers to develop targeted treatment plans.
基金supported by Shanghai Natural Science Foundation(Grant Number 22ZR1456600)Science and Technology Innovation Action Plan in Shanghai on Experimental Animal Research(Grant Number 22140903600)+1 种基金Clinical Research and Cultivation Project of Tongji Hospital in Shanghai(Grant Number ITJ(QN)2201)the National Natural Science Foundation of China(Nos.82227807,81830059)。
文摘Background:Sunitinib resistance is a major challenge in advanced renal cell carcinoma(RCC).Clinically,elucidating the underlying mechanisms and developing practical countermeasures for sunitinib resistance in RCC is desirable.In previous studies,we found that circAGAP1 expression was significantly upregulated in clear cell RCC(ccRCC)and was strongly associated with poor prognosis.However,the role of circAGAP1 in sunitinib resistance in ccRCC remains unclear.Methods:We used public databases for bioinformatics analysis to identify the binding targets of circAGAP1.Additionally,the effects of circAGAP1 on the proliferation,clonogenesis,apoptosis,and migration of ccRCC cells were analyzed using quantitative real-time PCR,cell counting kit-8 assays,migration and apoptosis assays,and colony formation assays.Furthermore,RNA immunoprecipitation,dual-luciferase reporter,and fluorescence in situ hybridization assays were used to explore the molecular mechanism.Results:In this study,circAGAP1 exhibited higher expression in sunitinib-sensitive ccRCC cells and inhibited the clonogenesis,proliferation,and migration of ccRCC cells after sunitinib treatment.Mechanical studies revealed that circAGAP1 regulated the expression of sunitinib target platelet-derived growth factor receptor by acting as a microRNA sponge that suppresses miR-149-5p,miR-455-5p,and miR-15a-5p simultaneously.Overexpression of these three miRNAs reversed circAGAP1-mediated sunitinib sensitivity in ccRCC.Conclusions:In summary,our findings indicate that circAGAP1 may serve as a promising biomarker to predict sunitinib sensibility and a therapeutic target in ccRCC.
