Objective： To search for a biomarker for colorectal cancer. Methods： The MSP, SSCP and deletion tests with serum have been taken simultaneously in 100 cases of colorectal cancer and 2 groups of controls, as well as ...Objective： To search for a biomarker for colorectal cancer. Methods： The MSP, SSCP and deletion tests with serum have been taken simultaneously in 100 cases of colorectal cancer and 2 groups of controls, as well as the specimens of 26 cancer tissues and 22 paracancerous tissues and 29 cases of benign disease tissues for a contrast. Results： The aberrant methylation rate of P16 in the serum was 69.00%, deletion rate 4.00% and suspicious point mutation rate 15.00% in colorectal cancer patients. The data of cancer tissues were the same as those of the serum, but in paracancerous tissue those were significantly lower. In 10 cases, sequencing analysis revealed that there were 3 cases of missense, one case of frameshift and one case of nonsense. Among them, four cases had P16 protein deletion. As a tumor marker, the sensitivity of combined use of three methods was 88.00%, specificity 96.87% and accuracy 90.15%. The combined use of MSP and SSCP could obtain the same results. Conclusion： The content of DNA in serum is minimal, but it reflects the tumor burden of patients. The 10^-3 fragments of DNA could be detected in the serum by MSP. It can be used in the clinical diagnosis or popular investigation, and long-term postoperative follow-up.展开更多
Objective To study on the CDKN2/P16gene in prim ary osteosarcoma.Method By using molecular biological methods that inclued genome DNA extraction from p araffined tissue and PCR-SSCP analy sis technique,we studied alte...Objective To study on the CDKN2/P16gene in prim ary osteosarcoma.Method By using molecular biological methods that inclued genome DNA extraction from p araffined tissue and PCR-SSCP analy sis technique,we studied alternations of CDKN2/P16gene in 25primary osteosarcomas.Results(1)The deletions frequency in differen tiation degree of osteosarcomas was①bone brood cell,16.7%;②cartilage brood cell,12.5%;③Fiber brood cell:20%,(P >0.05).(2)The deletion frequency in male patie nts was 17.6%,female pa-tients 12.5%,(P >0.05).(3)In early metastatic osteosarcomas t he deletion rate was 33.3%,which was significantly higher than that of th e control group with the rate of 10.5%(P <0.05).(4)The deletion rate was 16%and the muta tions were not found.Conclusion(1)The deletion rate was 16%and the mutatio ns were not found.This suggests that the deletions of CDKN2/P16gene were closely related to the genesis of primary osteosarcoma and that the main type of the alternation of CDKN2/P16gene was deletion.(2)In early metastatic osteosaarcomas the deletion rate was 33.3%,which was significantly higher than that of the c ontrol group with the rate of 10.5%.T his indicates to great extend that the deletions of CDKN2/P16gene were closely related to the metastat ic ability.(3)The deletions frequency had no signi ficant relationship with differentiation degree of oste osarcomas,so was with the sex of the p atient.展开更多
抑癌基因C D K N2/p16在结直肠癌等多种肿瘤中缺失、点突变及甲基化,导致肿瘤的发生发展。甲基化是其失活的主要机制。应用甲基化特异PC R,检测恶性肿瘤患者外周血中的甲基化改变,在早期诊断、监测术后复发和判定预后方面,具有重要的临...抑癌基因C D K N2/p16在结直肠癌等多种肿瘤中缺失、点突变及甲基化,导致肿瘤的发生发展。甲基化是其失活的主要机制。应用甲基化特异PC R,检测恶性肿瘤患者外周血中的甲基化改变,在早期诊断、监测术后复发和判定预后方面,具有重要的临床意义。展开更多
Objective: The molecular mechanism of prostate cancer is poorly understood. The aim of the study was to investigate the prevalence and prognostic value of promoter hypermethylation of retinoic acid receptor beta (RARB...Objective: The molecular mechanism of prostate cancer is poorly understood. The aim of the study was to investigate the prevalence and prognostic value of promoter hypermethylation of retinoic acid receptor beta (RARB) and p16 among benign prostatic hyperplasia (BPH) and prostate cancer patients. Methods: In this case-control study, 63 patients were included in three groups; 21 with BPH as the control group, 21 with prostate cancer and good prognostic factors (based on prostate-specific antigen, Gleason score and stage) as good prognosis group, and 21 with prostate cancer and poor prognostic features as poor prognosis group. The prostate biopsy specimen of each individual was examined for hypermethylation of RARB and p16 promoters by methylation specific PCR (MSPCR). Results: Seven (33.3%) patients with good prognosis and 15 (71.4%) patients with poor prognosis were positive for RARB methylation, which were significantly higher than controls (P <0.0001). p16 promoter methylation was shown in 19.0% and 47.6% patients with good and poor prognosis, respectively. The RARB and p16 promoter methylation in the poor prognosis group was significantly higher than that in the good prognosis group (P =0.02 for RARB and P<0.0001 for p16). Conclusion: Hypermethylation of RARB and p16 promoters may predict prognosis in prostate cancer.展开更多
AlM To investigate lor the first time p16/CDKN2 gene expres-sion at proten level in human gastric cancer.p16/CDKN2 gene is a newly identidied lumor suppresser gene and has been shown to be deleted,mutated,or rearrange...AlM To investigate lor the first time p16/CDKN2 gene expres-sion at proten level in human gastric cancer.p16/CDKN2 gene is a newly identidied lumor suppresser gene and has been shown to be deleted,mutated,or rearranged in numerous human tumors.Recently,homozygous deletion of the p16/CDKN2 gene has been reported in gastric cancer cell Iines with high Irequency,but none In surgical specimens ol gastric adenocarcinomas by Southern blot and SSCP analysis.METHODS The expression level ol p16 protein was analyzed informalin-loxed,paraffin-embedded tissue sections from 30 cases of gastric carcinomas and 20 cases of nomal gastric mucosa us-Ing a sensitive and specifie immunochemical technique with protein A-gold probe.RESULTS In normal controls all the samples expressed p16protein,the mean percenlage ol p16 protein-positive cells was 72.55%±17.22%(42%to 100%),whereas in cacer cases itwas 54.9%±28.9%which was much lower than that of nomalconlrols(P<0.05).Among cancer cases 23.33%(7/30)of specimens showed reduced expression of p16 protein and 10%(3/30)expressed no p16 protein.In additron,overexpression olp16 protein was also observed in one case.CONCLUSION Abnormal expression oi p16 protein 1s involvedIn gastric carcinoma and immunochemical technique may accu-rately reveal the expression of p16 protein,thus being more uselul In clinical sludies.展开更多
AIM To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis,depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gas...AIM To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis,depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma.METHODS The expression of P16 protein was examined by streptavidin-peroxidase conjugated method (S-P); the deletion and mutation of p16 gene were respectively examined by polymerase chain reaction (PCR) and polymerase chain reaction single-strand conformation polymorphism analysis (PCR-SSCP) in gastric carcinoma.RESULTS Expression of P16 protein was detected in 96.25% (77/80) of the normal gastric mucosa, in 92.00% (45/50) of the dysplastic gastric mucosa and in 47.54% (58/122) of the gastric carcinoma. The positive rate of P16 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P＜0.05). The positive rate of P16 protein expression in mucoid carcinoma 10.00% (1/ 10) was significantly lower than that in poorly differentiated carcinoma 51.22% ( 21/ 41 ),undifferentiated carcinoma 57.69% (15/26) and signet ring cell carcinoma 62.50% (10/ 16) (P＜0.05). The positive rate of p16 protein in 30 cases paired primary and lymph node metastatic gastric carcinoma: There was 46.67% (14/30) in primary gastric carcinoma, 16.67% (5/30) in lymph node metastatic gastric carcinoma. The positive rate of lymph node metastatic carcinoma was significantly lower than that of primary carcinoma (P＜0.05). There was of p16 gene mutation in exon 2, but 5 cases displayed deletion of p16 gene in exon 2 in the 25 primary gastric carcinomas.CONCLUSIONS The expression loss of P16 protein related to the gastric carcinogenesis, gastric carcinoma histopathological subtypes and lymph metastasis. The mutation of p16 gene in exon 2 may not be involved in gastric carcinogenesis. But the deletion of p16 gene in exon 2 may be involved in gastric carcinogenesis.展开更多
目的探讨原位间皮瘤(mesothelioma in situ,MIS)的形态特点并提高对这类病变的形态学认识。方法收集1例MIS患者的临床资料,观察其形态学特点,通过积液细胞学检查、组织活检、免疫组化染色及FISH检测,回顾性分析其形态学特征并复习文献...目的探讨原位间皮瘤(mesothelioma in situ,MIS)的形态特点并提高对这类病变的形态学认识。方法收集1例MIS患者的临床资料,观察其形态学特点,通过积液细胞学检查、组织活检、免疫组化染色及FISH检测,回顾性分析其形态学特征并复习文献。结果组织学活检和胸水细胞学检查中观察到两者存在明显的形态学差异,组织学形态良善温和,大小一致,细胞学形态异型明显,核大深染;免疫组化为D2-40、CK5/6、Calretinin、CK阳性表达,TTF-1阴性表达,BAP-1弱表达。FISH CDKN2A/P16纯合缺失。综合临床表现、影像学及胸腔镜的检查结果,符合MIS。结论MIS在浆膜腔积液和组织活检的形态可出现差异,诊断需结合临床表现、病理形态、免疫组化及相关基因检测结果综合评估。展开更多
基金This study was supported by a grant from Science and Research Foundation of Shanxi Province (No. 022075)
文摘Objective： To search for a biomarker for colorectal cancer. Methods： The MSP, SSCP and deletion tests with serum have been taken simultaneously in 100 cases of colorectal cancer and 2 groups of controls, as well as the specimens of 26 cancer tissues and 22 paracancerous tissues and 29 cases of benign disease tissues for a contrast. Results： The aberrant methylation rate of P16 in the serum was 69.00%, deletion rate 4.00% and suspicious point mutation rate 15.00% in colorectal cancer patients. The data of cancer tissues were the same as those of the serum, but in paracancerous tissue those were significantly lower. In 10 cases, sequencing analysis revealed that there were 3 cases of missense, one case of frameshift and one case of nonsense. Among them, four cases had P16 protein deletion. As a tumor marker, the sensitivity of combined use of three methods was 88.00%, specificity 96.87% and accuracy 90.15%. The combined use of MSP and SSCP could obtain the same results. Conclusion： The content of DNA in serum is minimal, but it reflects the tumor burden of patients. The 10^-3 fragments of DNA could be detected in the serum by MSP. It can be used in the clinical diagnosis or popular investigation, and long-term postoperative follow-up.
文摘Objective To study on the CDKN2/P16gene in prim ary osteosarcoma.Method By using molecular biological methods that inclued genome DNA extraction from p araffined tissue and PCR-SSCP analy sis technique,we studied alternations of CDKN2/P16gene in 25primary osteosarcomas.Results(1)The deletions frequency in differen tiation degree of osteosarcomas was①bone brood cell,16.7%;②cartilage brood cell,12.5%;③Fiber brood cell:20%,(P >0.05).(2)The deletion frequency in male patie nts was 17.6%,female pa-tients 12.5%,(P >0.05).(3)In early metastatic osteosarcomas t he deletion rate was 33.3%,which was significantly higher than that of th e control group with the rate of 10.5%(P <0.05).(4)The deletion rate was 16%and the muta tions were not found.Conclusion(1)The deletion rate was 16%and the mutatio ns were not found.This suggests that the deletions of CDKN2/P16gene were closely related to the genesis of primary osteosarcoma and that the main type of the alternation of CDKN2/P16gene was deletion.(2)In early metastatic osteosaarcomas the deletion rate was 33.3%,which was significantly higher than that of the c ontrol group with the rate of 10.5%.T his indicates to great extend that the deletions of CDKN2/P16gene were closely related to the metastat ic ability.(3)The deletions frequency had no signi ficant relationship with differentiation degree of oste osarcomas,so was with the sex of the p atient.
文摘Objective: The molecular mechanism of prostate cancer is poorly understood. The aim of the study was to investigate the prevalence and prognostic value of promoter hypermethylation of retinoic acid receptor beta (RARB) and p16 among benign prostatic hyperplasia (BPH) and prostate cancer patients. Methods: In this case-control study, 63 patients were included in three groups; 21 with BPH as the control group, 21 with prostate cancer and good prognostic factors (based on prostate-specific antigen, Gleason score and stage) as good prognosis group, and 21 with prostate cancer and poor prognostic features as poor prognosis group. The prostate biopsy specimen of each individual was examined for hypermethylation of RARB and p16 promoters by methylation specific PCR (MSPCR). Results: Seven (33.3%) patients with good prognosis and 15 (71.4%) patients with poor prognosis were positive for RARB methylation, which were significantly higher than controls (P <0.0001). p16 promoter methylation was shown in 19.0% and 47.6% patients with good and poor prognosis, respectively. The RARB and p16 promoter methylation in the poor prognosis group was significantly higher than that in the good prognosis group (P =0.02 for RARB and P<0.0001 for p16). Conclusion: Hypermethylation of RARB and p16 promoters may predict prognosis in prostate cancer.
文摘AlM To investigate lor the first time p16/CDKN2 gene expres-sion at proten level in human gastric cancer.p16/CDKN2 gene is a newly identidied lumor suppresser gene and has been shown to be deleted,mutated,or rearranged in numerous human tumors.Recently,homozygous deletion of the p16/CDKN2 gene has been reported in gastric cancer cell Iines with high Irequency,but none In surgical specimens ol gastric adenocarcinomas by Southern blot and SSCP analysis.METHODS The expression level ol p16 protein was analyzed informalin-loxed,paraffin-embedded tissue sections from 30 cases of gastric carcinomas and 20 cases of nomal gastric mucosa us-Ing a sensitive and specifie immunochemical technique with protein A-gold probe.RESULTS In normal controls all the samples expressed p16protein,the mean percenlage ol p16 protein-positive cells was 72.55%±17.22%(42%to 100%),whereas in cacer cases itwas 54.9%±28.9%which was much lower than that of nomalconlrols(P<0.05).Among cancer cases 23.33%(7/30)of specimens showed reduced expression of p16 protein and 10%(3/30)expressed no p16 protein.In additron,overexpression olp16 protein was also observed in one case.CONCLUSION Abnormal expression oi p16 protein 1s involvedIn gastric carcinoma and immunochemical technique may accu-rately reveal the expression of p16 protein,thus being more uselul In clinical sludies.
基金the grant from the Teaching Committee of HunanProvince,No.97B095the"8th 5-year Plan"of Health Department of Hunan Province,No.9301
文摘AIM To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis,depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma.METHODS The expression of P16 protein was examined by streptavidin-peroxidase conjugated method (S-P); the deletion and mutation of p16 gene were respectively examined by polymerase chain reaction (PCR) and polymerase chain reaction single-strand conformation polymorphism analysis (PCR-SSCP) in gastric carcinoma.RESULTS Expression of P16 protein was detected in 96.25% (77/80) of the normal gastric mucosa, in 92.00% (45/50) of the dysplastic gastric mucosa and in 47.54% (58/122) of the gastric carcinoma. The positive rate of P16 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P＜0.05). The positive rate of P16 protein expression in mucoid carcinoma 10.00% (1/ 10) was significantly lower than that in poorly differentiated carcinoma 51.22% ( 21/ 41 ),undifferentiated carcinoma 57.69% (15/26) and signet ring cell carcinoma 62.50% (10/ 16) (P＜0.05). The positive rate of p16 protein in 30 cases paired primary and lymph node metastatic gastric carcinoma: There was 46.67% (14/30) in primary gastric carcinoma, 16.67% (5/30) in lymph node metastatic gastric carcinoma. The positive rate of lymph node metastatic carcinoma was significantly lower than that of primary carcinoma (P＜0.05). There was of p16 gene mutation in exon 2, but 5 cases displayed deletion of p16 gene in exon 2 in the 25 primary gastric carcinomas.CONCLUSIONS The expression loss of P16 protein related to the gastric carcinogenesis, gastric carcinoma histopathological subtypes and lymph metastasis. The mutation of p16 gene in exon 2 may not be involved in gastric carcinogenesis. But the deletion of p16 gene in exon 2 may be involved in gastric carcinogenesis.
文摘目的探讨原位间皮瘤(mesothelioma in situ,MIS)的形态特点并提高对这类病变的形态学认识。方法收集1例MIS患者的临床资料,观察其形态学特点,通过积液细胞学检查、组织活检、免疫组化染色及FISH检测,回顾性分析其形态学特征并复习文献。结果组织学活检和胸水细胞学检查中观察到两者存在明显的形态学差异,组织学形态良善温和,大小一致,细胞学形态异型明显,核大深染;免疫组化为D2-40、CK5/6、Calretinin、CK阳性表达,TTF-1阴性表达,BAP-1弱表达。FISH CDKN2A/P16纯合缺失。综合临床表现、影像学及胸腔镜的检查结果,符合MIS。结论MIS在浆膜腔积液和组织活检的形态可出现差异,诊断需结合临床表现、病理形态、免疫组化及相关基因检测结果综合评估。
