Objective To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CD...Objective To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CDC25A expression in the cell cycle arrest induced by Art. Methods MTT method was employed to detect the proliferation of the Eca109 cells and normal human peripheral blood mononuclear cells (hPBMC) after Art treatment. Cell cycle of the tumor cells was assayed by flow cytometry. Inhibitory effects of Art on the transplanted tumor on nude mice were observed by mass weight, volume and morphological method. The expression of CDC25A in the Eca109 cells was examined by RT-PCR and Western blotting. Results Art significantly inhibited the proliferation of Eca109 with the IC50 of (68.80±0.76) μmol/L, while it had weaker effect on that of the hPBMC induced by Con A. At lower doses of Art, the number of Eca109 cells during G0/G1 was increased, and that at S phase was reduced dramatically. However, when the concentration was up to 100 μmol/L, most of cells were arrested at G2/M phase. The volume and weight of transplanted tumor receiving Art treatment were smaller and lower than those of control group, with the maximal inhibitory rate of 76.4%. Art dramatically inhibited the mRNA as well as protein expressions of CDC25A in the Eca109 cells. Conclusion Art can inhibit the proliferation of tumor cells and transplanted tumor without apparent side effect, possibly by the mechanism of modulating cell cycle through CDC25A down-regulation.展开更多
文摘Objective To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CDC25A expression in the cell cycle arrest induced by Art. Methods MTT method was employed to detect the proliferation of the Eca109 cells and normal human peripheral blood mononuclear cells (hPBMC) after Art treatment. Cell cycle of the tumor cells was assayed by flow cytometry. Inhibitory effects of Art on the transplanted tumor on nude mice were observed by mass weight, volume and morphological method. The expression of CDC25A in the Eca109 cells was examined by RT-PCR and Western blotting. Results Art significantly inhibited the proliferation of Eca109 with the IC50 of (68.80±0.76) μmol/L, while it had weaker effect on that of the hPBMC induced by Con A. At lower doses of Art, the number of Eca109 cells during G0/G1 was increased, and that at S phase was reduced dramatically. However, when the concentration was up to 100 μmol/L, most of cells were arrested at G2/M phase. The volume and weight of transplanted tumor receiving Art treatment were smaller and lower than those of control group, with the maximal inhibitory rate of 76.4%. Art dramatically inhibited the mRNA as well as protein expressions of CDC25A in the Eca109 cells. Conclusion Art can inhibit the proliferation of tumor cells and transplanted tumor without apparent side effect, possibly by the mechanism of modulating cell cycle through CDC25A down-regulation.
