目的研究人尿萃取物CDA-Ⅱ(cell d ifferentiation a-gent-Ⅱ,又名尿多酸肽)对人胶质瘤细胞SWO-38抑制增殖及诱导分化作用。方法应用MTT法、集落形成试验检测CDA-Ⅱ对SWO-38细胞增殖的影响;通过光镜观察和免疫组化鉴定细胞分化;利用裸...目的研究人尿萃取物CDA-Ⅱ(cell d ifferentiation a-gent-Ⅱ,又名尿多酸肽)对人胶质瘤细胞SWO-38抑制增殖及诱导分化作用。方法应用MTT法、集落形成试验检测CDA-Ⅱ对SWO-38细胞增殖的影响;通过光镜观察和免疫组化鉴定细胞分化;利用裸鼠抑瘤实验观察CDA-Ⅱ抗胶质瘤生长的作用。结果一定浓度的CDA-Ⅱ可使SWO-38细胞增殖与集落形成均明显受抑,并呈剂量依赖性。经1 g.L-1CDA-Ⅱ处理后,光镜观察发现SWO-38细胞胞体变大,核浆比减小,突起增多,表现出向成熟的星形细胞分化现象。免疫组化显示胶质纤维酸性蛋白(glial fibrillary ac id ic prote in,GFAP)表达增强。体内抑瘤试验见高、低剂量CDA-Ⅱ均可抑制裸鼠移植瘤生长,抑瘤率为79.94%、42.77%(P<0.05,n=10)。结论CDA-Ⅱ不仅能够抑制人胶质瘤SWO-38细胞的生长,而且能诱导SWO-38细胞分化,是一种很有应用前景的诱导分化剂。展开更多
目的:检测5种白血病细胞株中脱氧胞苷激酶(deoxycytidine kinase,DCK)和胞苷脱氨酶(cytidine deaminase,CDA)基因的单核苷酸多态性。方法:培养人红白血病细胞株K562,人慢性粒细胞白血病细胞株Ka,人急性髓系白血病细胞株HL-60、U937,人Bu...目的:检测5种白血病细胞株中脱氧胞苷激酶(deoxycytidine kinase,DCK)和胞苷脱氨酶(cytidine deaminase,CDA)基因的单核苷酸多态性。方法:培养人红白血病细胞株K562,人慢性粒细胞白血病细胞株Ka,人急性髓系白血病细胞株HL-60、U937,人Burkkit淋巴瘤细胞株Raji。采用QIAamp DNA Blood Mini Kit提取基因组DNA,设计引物,PCR扩增相应目的片段,采用基质辅助激光解吸电离飞行时间质谱技术(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOFMS)检测DCK基因A674G(rs111454937)、C1644T(rs72552079),CDA基因A79C(rs2072671)、G208A(rs60369023)的单核苷酸多态性。结果:5种细胞株DCK基因A674G(rs111454937)均为A/A纯合子型,C1644T(rs72552079)均为C/C纯合子型。HL-60、U937、Raji细胞株CDA基因A79C(rs2072671)为A/A纯合子,K562、Ka细胞株为C/A杂合子;5种细胞株CDA基因G208A(rs60369023)均为G/G纯合子型。结论:CDA基因的A79C(rs2072671)SNPs位点基因型在5株血液系统肿瘤细胞株中不尽相同,余所测3个SNPs位点基因型在各细胞株中均相同。展开更多
Since its birth in Britain in the late 1970s, critical discourse analysis （CDA, for short） has attracted great attention of western linguists. At present, some scholars in our country have devoted themselves to the ...Since its birth in Britain in the late 1970s, critical discourse analysis （CDA, for short） has attracted great attention of western linguists. At present, some scholars in our country have devoted themselves to the study of CDA and the study results are rewarding. However, some insufficiencies do exist in the research, such as the over-simplification of theories, excessive attention paid to theories rather than practice and neglect of nurturing critical thinking etc. Therefore, the author proposes to associate CDA with cognitive linguistics and interpret the research methods and the practical values of CDA in a wholly new perspective in terms of the analysis of the cohesion of the cognitive schema, ideology and notions from the information receivers and senders.展开更多
文摘目的研究人尿萃取物CDA-Ⅱ(cell d ifferentiation a-gent-Ⅱ,又名尿多酸肽)对人胶质瘤细胞SWO-38抑制增殖及诱导分化作用。方法应用MTT法、集落形成试验检测CDA-Ⅱ对SWO-38细胞增殖的影响;通过光镜观察和免疫组化鉴定细胞分化;利用裸鼠抑瘤实验观察CDA-Ⅱ抗胶质瘤生长的作用。结果一定浓度的CDA-Ⅱ可使SWO-38细胞增殖与集落形成均明显受抑,并呈剂量依赖性。经1 g.L-1CDA-Ⅱ处理后,光镜观察发现SWO-38细胞胞体变大,核浆比减小,突起增多,表现出向成熟的星形细胞分化现象。免疫组化显示胶质纤维酸性蛋白(glial fibrillary ac id ic prote in,GFAP)表达增强。体内抑瘤试验见高、低剂量CDA-Ⅱ均可抑制裸鼠移植瘤生长,抑瘤率为79.94%、42.77%(P<0.05,n=10)。结论CDA-Ⅱ不仅能够抑制人胶质瘤SWO-38细胞的生长,而且能诱导SWO-38细胞分化,是一种很有应用前景的诱导分化剂。
文摘目的:检测5种白血病细胞株中脱氧胞苷激酶(deoxycytidine kinase,DCK)和胞苷脱氨酶(cytidine deaminase,CDA)基因的单核苷酸多态性。方法:培养人红白血病细胞株K562,人慢性粒细胞白血病细胞株Ka,人急性髓系白血病细胞株HL-60、U937,人Burkkit淋巴瘤细胞株Raji。采用QIAamp DNA Blood Mini Kit提取基因组DNA,设计引物,PCR扩增相应目的片段,采用基质辅助激光解吸电离飞行时间质谱技术(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOFMS)检测DCK基因A674G(rs111454937)、C1644T(rs72552079),CDA基因A79C(rs2072671)、G208A(rs60369023)的单核苷酸多态性。结果:5种细胞株DCK基因A674G(rs111454937)均为A/A纯合子型,C1644T(rs72552079)均为C/C纯合子型。HL-60、U937、Raji细胞株CDA基因A79C(rs2072671)为A/A纯合子,K562、Ka细胞株为C/A杂合子;5种细胞株CDA基因G208A(rs60369023)均为G/G纯合子型。结论:CDA基因的A79C(rs2072671)SNPs位点基因型在5株血液系统肿瘤细胞株中不尽相同,余所测3个SNPs位点基因型在各细胞株中均相同。
文摘Since its birth in Britain in the late 1970s, critical discourse analysis （CDA, for short） has attracted great attention of western linguists. At present, some scholars in our country have devoted themselves to the study of CDA and the study results are rewarding. However, some insufficiencies do exist in the research, such as the over-simplification of theories, excessive attention paid to theories rather than practice and neglect of nurturing critical thinking etc. Therefore, the author proposes to associate CDA with cognitive linguistics and interpret the research methods and the practical values of CDA in a wholly new perspective in terms of the analysis of the cohesion of the cognitive schema, ideology and notions from the information receivers and senders.
