Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker i...Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog( ly75) was cloned and its expression pattern in gonads was analyzed. The full-length c DNA of ly75 was 7 346 bp, with an open reading frame(ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 k Da. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.展开更多
Background Dendritic ceils （DCs） are the most important professional antigen presenting cells that play a key role in initiating adaptive immune responses. The depletion and dysfunction of DCs contribute to the deve...Background Dendritic ceils （DCs） are the most important professional antigen presenting cells that play a key role in initiating adaptive immune responses. The depletion and dysfunction of DCs contribute to the development of immunodeficiency or immunoparalysis in some lung diseases. In the present study, we investigated the effects of Fms-like tyrosine kinase-3 ligand （FIt3L） administration in vivo on lung DCs expansion to provide an experimental basis of FIt3L used as a potential therapeutic agent for the related lung disorders. Methods Balb/c mice were randomly divided into FIt3L group （n=10） and control group （n=10）. Each mouse in the FIt3L group received subcutaneous administration of FIt3L at a dose of 10 pg once daily for nine consecutive days. Lung histology was observed, and CD11c and CD205 were immunologically labeled in lung tissue sections. Low-density lung cells were separated by density gradient centrifugation, and then subsets and MHC-II/I-Ad expression of DCs were analyzed by flow cytometry. Results In the FIt3L group the number and density of DC-like cells were markedly increased compared with the control group, mainly distributed in the alveolar septa. Immunological labeling in situ found that there were significantly higher numbers of CD11c＋ and CD205＋ DCs in lung mesenchymal tissue （P 〈0.05）, where they formed a denser reticular formation. Flow cytometry analysis demonstrated that the proportions of myeloid CD11c＋CD11b＋ DCs and plasmacytoid CD11c＋CD45R/B220＋ DCs in the low-density lung cells in the FIt3L group were significantly higher compared with the control group; showing 3.17- and 3.3-fold increase respectively （P 〈0.05）. The proportion of CD11c＋ DCs expressing MHC-II/I-Ad＋ was significantly increased, with a 2.7-fold increase as compared with the control group （P 〈0.05）. Conclusions FIt3L administration in vivo induces lung DCs expansion, favoring myeloid and plasmacytoid DC subsets, which are phenotypically more mature. FIt3L may be useful in the therapy to augment immune function of the lung.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31372514,31472264,31572602)the China Agriculture Research System(No.CARS-47)+1 种基金the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology(Nos.2015ASKJ02,2015ASKJ02-03-03)the Youth Innovation Promotion Association CAS and Chinese Academy of Science and Technology Service Network Planning(No.KFJ-EW-STS-060)
文摘Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog( ly75) was cloned and its expression pattern in gonads was analyzed. The full-length c DNA of ly75 was 7 346 bp, with an open reading frame(ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 k Da. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.
基金This research was supported by the grant from the National Natural Science Foundation of China （No. 81000848）.
文摘Background Dendritic ceils （DCs） are the most important professional antigen presenting cells that play a key role in initiating adaptive immune responses. The depletion and dysfunction of DCs contribute to the development of immunodeficiency or immunoparalysis in some lung diseases. In the present study, we investigated the effects of Fms-like tyrosine kinase-3 ligand （FIt3L） administration in vivo on lung DCs expansion to provide an experimental basis of FIt3L used as a potential therapeutic agent for the related lung disorders. Methods Balb/c mice were randomly divided into FIt3L group （n=10） and control group （n=10）. Each mouse in the FIt3L group received subcutaneous administration of FIt3L at a dose of 10 pg once daily for nine consecutive days. Lung histology was observed, and CD11c and CD205 were immunologically labeled in lung tissue sections. Low-density lung cells were separated by density gradient centrifugation, and then subsets and MHC-II/I-Ad expression of DCs were analyzed by flow cytometry. Results In the FIt3L group the number and density of DC-like cells were markedly increased compared with the control group, mainly distributed in the alveolar septa. Immunological labeling in situ found that there were significantly higher numbers of CD11c＋ and CD205＋ DCs in lung mesenchymal tissue （P 〈0.05）, where they formed a denser reticular formation. Flow cytometry analysis demonstrated that the proportions of myeloid CD11c＋CD11b＋ DCs and plasmacytoid CD11c＋CD45R/B220＋ DCs in the low-density lung cells in the FIt3L group were significantly higher compared with the control group; showing 3.17- and 3.3-fold increase respectively （P 〈0.05）. The proportion of CD11c＋ DCs expressing MHC-II/I-Ad＋ was significantly increased, with a 2.7-fold increase as compared with the control group （P 〈0.05）. Conclusions FIt3L administration in vivo induces lung DCs expansion, favoring myeloid and plasmacytoid DC subsets, which are phenotypically more mature. FIt3L may be useful in the therapy to augment immune function of the lung.
