CD134,a member of the tumor necrosis factor receptor superfamily,plays a crucial role in T cell survival.In this study,peripheral blood mononuclear cells(PBMCs)stimulated with phytohemagglutinin(PHA,50μg/ml)were trea...CD134,a member of the tumor necrosis factor receptor superfamily,plays a crucial role in T cell survival.In this study,peripheral blood mononuclear cells(PBMCs)stimulated with phytohemagglutinin(PHA,50μg/ml)were treated by CD134 mAb(1μg/ml,5μg/ml,10μg/ml)for 6 h,12 h,24 h and 48 h.The level of perforin mRNA was measured by reverse transcription-polymerase chain reaction(RT-PCR)technique.Our data showed that the expression of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in range of 1μg/ml to 5μg/ml and dropped down to its minimum on 24 h(p〈0.05).The level of perforin mRNA reached a plateau when the concentration of CD134 mAb exceeded 5μg/ml.In conclusion,CD134 mAb can inhibit perforin expression,which may enhance the ability of T cells for survival.Cellular&Molecular Immunology.2005;2(6):467-471.展开更多
基金supported by the Science and Technology Development Plan Program of Shandong Province of China under Grant(No.200328).
文摘CD134,a member of the tumor necrosis factor receptor superfamily,plays a crucial role in T cell survival.In this study,peripheral blood mononuclear cells(PBMCs)stimulated with phytohemagglutinin(PHA,50μg/ml)were treated by CD134 mAb(1μg/ml,5μg/ml,10μg/ml)for 6 h,12 h,24 h and 48 h.The level of perforin mRNA was measured by reverse transcription-polymerase chain reaction(RT-PCR)technique.Our data showed that the expression of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in range of 1μg/ml to 5μg/ml and dropped down to its minimum on 24 h(p〈0.05).The level of perforin mRNA reached a plateau when the concentration of CD134 mAb exceeded 5μg/ml.In conclusion,CD134 mAb can inhibit perforin expression,which may enhance the ability of T cells for survival.Cellular&Molecular Immunology.2005;2(6):467-471.
