Calcium(Ca)is essential for plant growth and stress adaptation,yet its availability is often limited in acidic soils,posing a major threat to crop production.Understanding the intricate mechanisms orchestrating plant ...Calcium(Ca)is essential for plant growth and stress adaptation,yet its availability is often limited in acidic soils,posing a major threat to crop production.Understanding the intricate mechanisms orchestrating plant adaptation to Ca deficiency remains elusive.Here,we show that the Ca deficiency-enhanced nuclear accumulation of the transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1(STOP1)in Arabidopsis thaliana confers tolerance to Ca deprivation,with the global transcriptional responses triggered by Ca deprivation largely impaired in the stop1 mutant.Notably,STOP1 activates the Ca deprivation-induced expression of CATION/Ca^(2+)EXCHANGER 1(CCX1)by directly binding to its promoter region,which facilitates Ca^(2+)efflux from endoplasmic reticulum to cytosol to maintain Ca homeostasis.Consequently,the constitutive expression of CCX1 in the stop1 mutant partially rescues the Ca deficiency phenotype by increasing Ca content in the shoots.These findings uncover the pivotal role of the STOP1-CCX1 axis in plant adaptation to low Ca,offering alternative manipulating strategies to improve plant Ca nutrition in acidic soils and extending our understanding of the multifaceted role of STOP1.展开更多
基金supported by National Natural Science Foundation of China(32201702)Central Public-interest Scientific Institution Basal Research Fund(No.Y2023QC21)+3 种基金Natural Science Foundation of Zhejiang Province(LY23C130005)National Key Research and Development Program of China(2023YFD1901800,2023YFD1902905)the Agricultural Science and Technology Innovation Program(ASTIP)the Postdoctoral Fellowship Program of CPSF(Grant No.GZC20232327)to J.Y.
文摘Calcium(Ca)is essential for plant growth and stress adaptation,yet its availability is often limited in acidic soils,posing a major threat to crop production.Understanding the intricate mechanisms orchestrating plant adaptation to Ca deficiency remains elusive.Here,we show that the Ca deficiency-enhanced nuclear accumulation of the transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1(STOP1)in Arabidopsis thaliana confers tolerance to Ca deprivation,with the global transcriptional responses triggered by Ca deprivation largely impaired in the stop1 mutant.Notably,STOP1 activates the Ca deprivation-induced expression of CATION/Ca^(2+)EXCHANGER 1(CCX1)by directly binding to its promoter region,which facilitates Ca^(2+)efflux from endoplasmic reticulum to cytosol to maintain Ca homeostasis.Consequently,the constitutive expression of CCX1 in the stop1 mutant partially rescues the Ca deficiency phenotype by increasing Ca content in the shoots.These findings uncover the pivotal role of the STOP1-CCX1 axis in plant adaptation to low Ca,offering alternative manipulating strategies to improve plant Ca nutrition in acidic soils and extending our understanding of the multifaceted role of STOP1.
