Chaperonins assist the proper folding of target proteins without being a part of the substrates. The eukaryotic cytosolic chaperonin, CCT-Chaperonin Containing TCP-1 (tailless complex polypeptide-1), is mainly involve...Chaperonins assist the proper folding of target proteins without being a part of the substrates. The eukaryotic cytosolic chaperonin, CCT-Chaperonin Containing TCP-1 (tailless complex polypeptide-1), is mainly involved in the formation of cytoskeletal proteins and is essential for cell viability. Mammalian CCT is commonly a protein complex composed of 7-9 subunit species. We have isolated a novel full-length cDNA from human testis cDNA library. This cDNA of 1935 bp contains a 1605 bp open reading frame (ORF) encoding 535 amino acids (aa). The deduced protein of the cDNA is highly homologous to the CCTβ subunit of saccharomyces cerevisiae, schizosaccharomyces pombe, caenorhabditis elegans and mouse, etc. Especially high homology (97%) is found between the deduced protein and mouse CCTβhomology, the protein encoded by the new gene was proposed to be a human CCTβ subunit. Northern hybridization showed that human CCTβ gene is expressed as a transcript of about 2.0 kb in various tissues. Overexpression展开更多
Tremendous advances has been witnessed in the past few years in the lanthanide complexes mediated coordinative chain transfer polymerization(CCTP) of conjugated dienes. CCTP features catalyst economy, well-controlling...Tremendous advances has been witnessed in the past few years in the lanthanide complexes mediated coordinative chain transfer polymerization(CCTP) of conjugated dienes. CCTP features catalyst economy, well-controlling over both microstructure and architecture of the resulting polymers, and accessibility for novel(co)polymers. This review highlights the recent progresses made in the field of CCTP of dienes. After a brief introduction, the body of this review is divided into three parts:(1) principle of CCTP;(2) coordinative chain transfer homopolymerization of dienes;(3) coordinative chain transfer copolymerization of dienes.At the end, we present some challenges remaining in this area and our personal opinion regarding where this field should continue to develop. CCTP represents a novel strategy to prepare polydiene synthetic rubbers with controlled high molecular weight and narrow molecular weight distribution, which has reached the practical industrial application level, demonstrating a great potential in industrialization.展开更多
文摘Chaperonins assist the proper folding of target proteins without being a part of the substrates. The eukaryotic cytosolic chaperonin, CCT-Chaperonin Containing TCP-1 (tailless complex polypeptide-1), is mainly involved in the formation of cytoskeletal proteins and is essential for cell viability. Mammalian CCT is commonly a protein complex composed of 7-9 subunit species. We have isolated a novel full-length cDNA from human testis cDNA library. This cDNA of 1935 bp contains a 1605 bp open reading frame (ORF) encoding 535 amino acids (aa). The deduced protein of the cDNA is highly homologous to the CCTβ subunit of saccharomyces cerevisiae, schizosaccharomyces pombe, caenorhabditis elegans and mouse, etc. Especially high homology (97%) is found between the deduced protein and mouse CCTβhomology, the protein encoded by the new gene was proposed to be a human CCTβ subunit. Northern hybridization showed that human CCTβ gene is expressed as a transcript of about 2.0 kb in various tissues. Overexpression
基金supported by the National Key R&D Program of China(Grant Nos.2017YFB0307100,2017YFB0307103)the National Basic Research Program of China(Grant Nos.2015CB654700,2015CB654702)
文摘Tremendous advances has been witnessed in the past few years in the lanthanide complexes mediated coordinative chain transfer polymerization(CCTP) of conjugated dienes. CCTP features catalyst economy, well-controlling over both microstructure and architecture of the resulting polymers, and accessibility for novel(co)polymers. This review highlights the recent progresses made in the field of CCTP of dienes. After a brief introduction, the body of this review is divided into three parts:(1) principle of CCTP;(2) coordinative chain transfer homopolymerization of dienes;(3) coordinative chain transfer copolymerization of dienes.At the end, we present some challenges remaining in this area and our personal opinion regarding where this field should continue to develop. CCTP represents a novel strategy to prepare polydiene synthetic rubbers with controlled high molecular weight and narrow molecular weight distribution, which has reached the practical industrial application level, demonstrating a great potential in industrialization.
