[目的]探讨肾细胞癌中TXNDC5的表达及其对786-O细胞增殖、侵袭和迁移的影响。[方法]2020年8月-2023年8月102例肾细胞癌患者纳入研究,免疫组化染色测定TXNDC5表达水平,χ^(2)检验分析TXNDC5表达与肾细胞癌临床病理特征的关系。同时设肾...[目的]探讨肾细胞癌中TXNDC5的表达及其对786-O细胞增殖、侵袭和迁移的影响。[方法]2020年8月-2023年8月102例肾细胞癌患者纳入研究,免疫组化染色测定TXNDC5表达水平,χ^(2)检验分析TXNDC5表达与肾细胞癌临床病理特征的关系。同时设肾细胞癌细胞786-O组、sh-NC组、TXNDC5-inhibitor组、TXNDC5-mimics组,测定各组细胞增殖、侵袭、凋亡以及TXNDC5、CCN2水平。[结果]肾细胞癌组TXNDC5蛋白表达阳性率高于癌旁组(P<0.05)。TXNDC5蛋白表达与肾细胞癌临床病理特征相关。TXNDC5-inhibitor组OD值(0.78±0.05 vs 0.41±0.03)、存活率(78.56%±6.18%vs 42.11%±7.24%)、单克隆形成数目[(619.33±89.20)/个]、穿膜数[(323.85±84.25 vs 93.85±95.34)/个]、TXNDC5、CCN2 mRNA和蛋白表达水平低于肾细胞癌细胞786-O组、sh-NC组,凋亡率高于肾细胞癌细胞786-O组、sh-NC组(P<0.05);TXNDC5-mimics组OD值、存活率、单克隆形成数目、穿膜数、TXNDC5、CCN2 mRNA和蛋白表达水平高于肾细胞癌细胞786-O组、sh-NC组,凋亡率低于肾细胞癌细胞786-O组、sh-NC组(P<0.05);TXNDC5-mimics组OD值、存活率、单克隆形成数目、穿膜数、TXNDC5、CCN2 mRNA和蛋白表达水平高于TXNDC5-inhibitor组,凋亡率低于TXNDC5-inhibitor组(P<0.05)。[结论]TXNDC5下调可以抑制肾细胞癌细胞的增殖、迁移和侵袭,同时诱导细胞凋亡,这一过程与调节CCN2相关。展开更多
The sustainable geotechnical approach for addressing the challenges associated with clayey soils at construction sites involves the modification of these soils’mechanical and chemical characteristics using soil enhan...The sustainable geotechnical approach for addressing the challenges associated with clayey soils at construction sites involves the modification of these soils’mechanical and chemical characteristics using soil enhancement methods.The present study investigates the coupling effect of sandstone slurry waste(SSW)and calcium carbonate nanoparticle(CCN)as potential stabilizers to enhance the characteristics of clayey soil.A comprehensive investigation was conducted using compaction tests,plasticity index(PI)tests,California bearing ratio(CBR)tests,unconfined compressive strength(UCS)tests,and microstructural analyses of clayey soil,SSW and SSW-CCN-treated clay samples containing 5%,10%,15%,20%,25%,30%,and 35%SSW and 0.3%,0.6%,0.9%,1.2%,and 1.5%CCN mixed with clayey soil in different combinations of clay,SSW,and CCN.The findings reveal that incorporating 25%SSW with 0.9%CCN into clay soil results in an increase in the UCS from 132.2 kPa for untreated clayey soil without curing to 263 kPa after a 28-d curing period.Similarly,a rising trend in CBR results is observed up to 25%SSW addition in clay soil and up to 0.9%CCN addition in SSW-clay mixture.Initially,notable enhancements in UCS were attributed to a denser soil structure,followed by the formation of calcium–silicate–hydrate(CSH)gel,which intensified with prolonged curing.Gel patches were detected by scanning electron microscopy(SEM)in addition to particle aggregation.The results obtained from thermogravimetric analysis,Fourier transform infrared spectroscopy(FTIR),and X-ray diffraction(XRD)supported the presence of hydration products such as CSH.The experimental study indicates that SSW,in combination with CCN,offers a sustainable alternative to traditional soil stabilizers.展开更多
文摘目的探讨中晚期孕妇血清NOV/CCN3水平变化,并分析其与妊娠期糖尿病(gestational diabetes mellitus,GDM)及妊娠结局的关系。方法根据口服葡萄糖耐量实验(oral glucose tolerance test,OGTT)结果,将252例妊娠女性分为GDM组与对照组。根据妊娠前体质量指数水平及妊娠结局情况将GDM组进行分组。收集所有研究对象临床资料,比较组间一般资料、生化指标及NOV/CCN3水平的差异。结果GDM组血清NOV/CCN3水平高于对照组(P<0.001)。Spearman相关分析结果表明血清NOV/CCN3与妊娠前体质量、妊娠前体质量指数、胰岛素抵抗指数、总胆固醇呈正相关,与胰岛素敏感指数呈负相关(P<0.05)。logistic回归分析结果表明,NOV/CCN3是GDM发生的独立危险因素[OR=1.097,95%CI(1.020~1.179),P=0.013],且是GDM患者发生不良妊娠结局的独立危险因素[OR=1.032,95%CI(1.020~1.045),P<0.001],ROC分析结果显示曲线下面积(area under the curve,AUC)分别为0.840、0.784(P<0.05)。结论中晚期孕妇血清NOV/CCN3水平与肥胖、胰岛素抵抗及糖脂代谢相关,参与GDM发生发展,为评估中晚期孕妇发生GDM及GDM患者妊娠结局提供新的思路。
文摘[目的]探讨肾细胞癌中TXNDC5的表达及其对786-O细胞增殖、侵袭和迁移的影响。[方法]2020年8月-2023年8月102例肾细胞癌患者纳入研究,免疫组化染色测定TXNDC5表达水平,χ^(2)检验分析TXNDC5表达与肾细胞癌临床病理特征的关系。同时设肾细胞癌细胞786-O组、sh-NC组、TXNDC5-inhibitor组、TXNDC5-mimics组,测定各组细胞增殖、侵袭、凋亡以及TXNDC5、CCN2水平。[结果]肾细胞癌组TXNDC5蛋白表达阳性率高于癌旁组(P<0.05)。TXNDC5蛋白表达与肾细胞癌临床病理特征相关。TXNDC5-inhibitor组OD值(0.78±0.05 vs 0.41±0.03)、存活率(78.56%±6.18%vs 42.11%±7.24%)、单克隆形成数目[(619.33±89.20)/个]、穿膜数[(323.85±84.25 vs 93.85±95.34)/个]、TXNDC5、CCN2 mRNA和蛋白表达水平低于肾细胞癌细胞786-O组、sh-NC组,凋亡率高于肾细胞癌细胞786-O组、sh-NC组(P<0.05);TXNDC5-mimics组OD值、存活率、单克隆形成数目、穿膜数、TXNDC5、CCN2 mRNA和蛋白表达水平高于肾细胞癌细胞786-O组、sh-NC组,凋亡率低于肾细胞癌细胞786-O组、sh-NC组(P<0.05);TXNDC5-mimics组OD值、存活率、单克隆形成数目、穿膜数、TXNDC5、CCN2 mRNA和蛋白表达水平高于TXNDC5-inhibitor组,凋亡率低于TXNDC5-inhibitor组(P<0.05)。[结论]TXNDC5下调可以抑制肾细胞癌细胞的增殖、迁移和侵袭,同时诱导细胞凋亡,这一过程与调节CCN2相关。
文摘The sustainable geotechnical approach for addressing the challenges associated with clayey soils at construction sites involves the modification of these soils’mechanical and chemical characteristics using soil enhancement methods.The present study investigates the coupling effect of sandstone slurry waste(SSW)and calcium carbonate nanoparticle(CCN)as potential stabilizers to enhance the characteristics of clayey soil.A comprehensive investigation was conducted using compaction tests,plasticity index(PI)tests,California bearing ratio(CBR)tests,unconfined compressive strength(UCS)tests,and microstructural analyses of clayey soil,SSW and SSW-CCN-treated clay samples containing 5%,10%,15%,20%,25%,30%,and 35%SSW and 0.3%,0.6%,0.9%,1.2%,and 1.5%CCN mixed with clayey soil in different combinations of clay,SSW,and CCN.The findings reveal that incorporating 25%SSW with 0.9%CCN into clay soil results in an increase in the UCS from 132.2 kPa for untreated clayey soil without curing to 263 kPa after a 28-d curing period.Similarly,a rising trend in CBR results is observed up to 25%SSW addition in clay soil and up to 0.9%CCN addition in SSW-clay mixture.Initially,notable enhancements in UCS were attributed to a denser soil structure,followed by the formation of calcium–silicate–hydrate(CSH)gel,which intensified with prolonged curing.Gel patches were detected by scanning electron microscopy(SEM)in addition to particle aggregation.The results obtained from thermogravimetric analysis,Fourier transform infrared spectroscopy(FTIR),and X-ray diffraction(XRD)supported the presence of hydration products such as CSH.The experimental study indicates that SSW,in combination with CCN,offers a sustainable alternative to traditional soil stabilizers.
