This study investigated the effects of Lactiplantibacillus plantarum CCFM1296 postbiotics(non-viable microbial cells or their components and fermentation supernatant)on skin aging via endogenous hyaluronic acid(HA)reg...This study investigated the effects of Lactiplantibacillus plantarum CCFM1296 postbiotics(non-viable microbial cells or their components and fermentation supernatant)on skin aging via endogenous hyaluronic acid(HA)regulation.In vitro screening revealed that L.plantarum CCFM1296 postbiotic significantly upregulated HA synthase mRNA expression in human epidermal keratinocyte(HaCaT)cells.In a D-galactose-induced aging model(n=5),oral administration of CCFM1296 postbiotic increased skin HA content by 13.56%(P=0.0009),hydration by 19.77%(P=0.0121),and elasticity by 31.32%(P=0.0122)compared to the model group.Bacterial components(CCFM1296-B),rather than the fermentation supernatant,upregulated UGDH and HAS2(both mRNA and protein),reduced oxidative stress and inflammation.Skin metabolomics identified 24 bacterial metabolites that were enriched in skin tissue following CCFM1296-B intervention.These findings demonstrate that L.plantarum CCFM1296 postbiotics ameliorate skin aging,which is associated with enhanced endogenous HA synthesis.展开更多
基金supported by the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.
文摘This study investigated the effects of Lactiplantibacillus plantarum CCFM1296 postbiotics(non-viable microbial cells or their components and fermentation supernatant)on skin aging via endogenous hyaluronic acid(HA)regulation.In vitro screening revealed that L.plantarum CCFM1296 postbiotic significantly upregulated HA synthase mRNA expression in human epidermal keratinocyte(HaCaT)cells.In a D-galactose-induced aging model(n=5),oral administration of CCFM1296 postbiotic increased skin HA content by 13.56%(P=0.0009),hydration by 19.77%(P=0.0121),and elasticity by 31.32%(P=0.0122)compared to the model group.Bacterial components(CCFM1296-B),rather than the fermentation supernatant,upregulated UGDH and HAS2(both mRNA and protein),reduced oxidative stress and inflammation.Skin metabolomics identified 24 bacterial metabolites that were enriched in skin tissue following CCFM1296-B intervention.These findings demonstrate that L.plantarum CCFM1296 postbiotics ameliorate skin aging,which is associated with enhanced endogenous HA synthesis.
