2015年6月26日,来自全国各地众多专家及医生相聚美丽山城重庆,拉开了一年一度的“中国胎儿医学大会”序幕。今年已经是第五届了,回首胎儿医学大会走过的四个年头,无论是以“Back to the basic”为主题的第一届,还是以“双胎妊娠”、“...2015年6月26日,来自全国各地众多专家及医生相聚美丽山城重庆,拉开了一年一度的“中国胎儿医学大会”序幕。今年已经是第五届了,回首胎儿医学大会走过的四个年头,无论是以“Back to the basic”为主题的第一届,还是以“双胎妊娠”、“出生缺陷”为主题的第二届,或是着重介绍“产前筛查与分子诊断”、“NIPT”等专题的第三、第四届,我们都迅速捕捉着胎儿医学的最新热点,展开更多
Osteoporosis represents a growing public health challenge in ageing societies,resulting in serious health threats and economic burdens.Fermented dairy products have shown the potential as an intervention strategy for ...Osteoporosis represents a growing public health challenge in ageing societies,resulting in serious health threats and economic burdens.Fermented dairy products have shown the potential as an intervention strategy for osteoporosis.The present work aimed to evaluate the effect of whey from fermented milk by Lactobacillus hel-veticus in ovariectomized rats.The results demonstrated that whey from L.helveticus CCFM1263-fermented milk markedly increased bone mineral density,restored trabecular microarchitecture(p<0.05),as well as prevented fluctuations in bone turnover markers.Based on metabolomic analysis,whey from fermented milk by L.helveticus CCFM1263 significantly reshaped the systemic metabolic profile(p<0.05),and increased the levels of aden-osine,arginine,and tryptophan.Additionally,the intervention effectively reversed the ovariectomy-induced depletion of serum bile acids,specifically restoring cholic acid and lithocholic acid levels.Together,our re-sults suggested that whey from fermented milk by L.helveticus CCFM1263 ameliorated osteoporosis and had potential for intervene with the onset and progression of postmenopausal osteoporosis.展开更多
The development of allergic rhinitis(AR)is intimately associated with disturbances in intestinal microbiota and abnormalities in tryptophan(Trp)metabolism.Probiotics have been shown to effectively modulate the intesti...The development of allergic rhinitis(AR)is intimately associated with disturbances in intestinal microbiota and abnormalities in tryptophan(Trp)metabolism.Probiotics have been shown to effectively modulate the intestinal microecology and alleviate AR symptoms.However,current research has yet to elucidate whether probiotics are linked to metabolites of the Trp-indole pathway in their capacity to ameliorate AR.This study aimed to investigate the alleviating effects of probiotics that produce abundant indole derivatives on female specific-pathogenfree grade BALB/c mice with AR.The results demonstrated that the ameliorative effect of probiotics on AR was strain-dependent.Notably,Bifidobacterium longum subspecies infantis CCFM111 enhanced the abundance of beneficial bacteria Akkermansia and Ruminiclostridium 6,and significantly elevated indole-3-carboxaldehyde(IAld)levels(p<0.001)in the intestines of AR mice.Concurrently with the up-regulation of IAld,which activates the aryl hydrocarbon receptor and modulates immune responses,in the CCFM111-treated AR mice,the levels of ovalbumin-specific immunoglobulin E(IgE)were markedly down-regulated(p<0.001).Similarly,total IgE levels(p<0.05)and pro-inflammatory cytokines(interleukin(IL)-4,IL-13,IL-6,and IL-17;p<0.01)were significantly reduced.Conversely,the concentration of the anti-inflammatory cytokine IL-10 was significantly increased(p<0.01).Collectively,these alterations effectively mitigated allergic symptoms,lung inflammation,and prevented body weight loss in AR mice.In conclusion,our findings suggested that IAld may serve as an efficacious material foundation for alleviating AR through the administration of probiotics.This provided a strong scientific rationale for the development of probiotic resources with AR-mitigating properties.展开更多
Reducing intestinal oxidative stress presents a promising approach to mitigating intestinal senescence.Lactiplantibacillus plantarum CCFM8610 has been reported to attenuate cadmium-induced intestinal dysfunction and d...Reducing intestinal oxidative stress presents a promising approach to mitigating intestinal senescence.Lactiplantibacillus plantarum CCFM8610 has been reported to attenuate cadmium-induced intestinal dysfunction and demonstrate significant antioxidant properties.However,its effectiveness and underlying mechanisms in modulating intestinal senescence are not fully elucidated.In this study,we initially observed a marked increase in intestinal permeability and a notable elevation in oxidative stress levels in induced senescent mice.Supplementation with CCFM8610 notably alleviated these symptoms and enhanced intestinal barrier integrity.Subsequently,analysis of different parts(<3 kDa and>3 kDa)of the CCFM8610 fermentation supernatant fractions and non-targeted metabolomics data identified indole-3-lactic acid(ILA)as a potentially critical component.ILA was found to significantly reduce elevated oxidative stress levels in senescent NCM460 cells and mitigate cellular senescence.Mechanically,ILA activated adenosine 5′-monophosphate-activated protein kinase(AMPK)signaling,promoted nuclear Factor erythroid 2-Related Factor 2(NRF2)nuclear expression,reduced oxidative stress,and improved cell viability,thereby alleviating intestinal senescence.These findings indicate that CCFM8610 shows significant potential in addressing intestinal senescence,with ILA identified as a key bioactive compound responsible for these effects.展开更多
This study investigated the effects of Lactiplantibacillus plantarum CCFM1296 postbiotics(non-viable microbial cells or their components and fermentation supernatant)on skin aging via endogenous hyaluronic acid(HA)reg...This study investigated the effects of Lactiplantibacillus plantarum CCFM1296 postbiotics(non-viable microbial cells or their components and fermentation supernatant)on skin aging via endogenous hyaluronic acid(HA)regulation.In vitro screening revealed that L.plantarum CCFM1296 postbiotic significantly upregulated HA synthase mRNA expression in human epidermal keratinocyte(HaCaT)cells.In a D-galactose-induced aging model(n=5),oral administration of CCFM1296 postbiotic increased skin HA content by 13.56%(P=0.0009),hydration by 19.77%(P=0.0121),and elasticity by 31.32%(P=0.0122)compared to the model group.Bacterial components(CCFM1296-B),rather than the fermentation supernatant,upregulated UGDH and HAS2(both mRNA and protein),reduced oxidative stress and inflammation.Skin metabolomics identified 24 bacterial metabolites that were enriched in skin tissue following CCFM1296-B intervention.These findings demonstrate that L.plantarum CCFM1296 postbiotics ameliorate skin aging,which is associated with enhanced endogenous HA synthesis.展开更多
文摘2015年6月26日,来自全国各地众多专家及医生相聚美丽山城重庆,拉开了一年一度的“中国胎儿医学大会”序幕。今年已经是第五届了,回首胎儿医学大会走过的四个年头,无论是以“Back to the basic”为主题的第一届,还是以“双胎妊娠”、“出生缺陷”为主题的第二届,或是着重介绍“产前筛查与分子诊断”、“NIPT”等专题的第三、第四届,我们都迅速捕捉着胎儿医学的最新热点,
基金supported by the National Natural Science Foundation of China(No.32472347)Jiangsu Provincial Policy Guidance Programme-International Cooperation Projects(No.BZ2024045).
文摘Osteoporosis represents a growing public health challenge in ageing societies,resulting in serious health threats and economic burdens.Fermented dairy products have shown the potential as an intervention strategy for osteoporosis.The present work aimed to evaluate the effect of whey from fermented milk by Lactobacillus hel-veticus in ovariectomized rats.The results demonstrated that whey from L.helveticus CCFM1263-fermented milk markedly increased bone mineral density,restored trabecular microarchitecture(p<0.05),as well as prevented fluctuations in bone turnover markers.Based on metabolomic analysis,whey from fermented milk by L.helveticus CCFM1263 significantly reshaped the systemic metabolic profile(p<0.05),and increased the levels of aden-osine,arginine,and tryptophan.Additionally,the intervention effectively reversed the ovariectomy-induced depletion of serum bile acids,specifically restoring cholic acid and lithocholic acid levels.Together,our re-sults suggested that whey from fermented milk by L.helveticus CCFM1263 ameliorated osteoporosis and had potential for intervene with the onset and progression of postmenopausal osteoporosis.
基金supported by the National Key Research and Development Program of China(No.2022YFF1100203)the collaborative innovation center of food safety and quality control in Jiangsu Province.
文摘The development of allergic rhinitis(AR)is intimately associated with disturbances in intestinal microbiota and abnormalities in tryptophan(Trp)metabolism.Probiotics have been shown to effectively modulate the intestinal microecology and alleviate AR symptoms.However,current research has yet to elucidate whether probiotics are linked to metabolites of the Trp-indole pathway in their capacity to ameliorate AR.This study aimed to investigate the alleviating effects of probiotics that produce abundant indole derivatives on female specific-pathogenfree grade BALB/c mice with AR.The results demonstrated that the ameliorative effect of probiotics on AR was strain-dependent.Notably,Bifidobacterium longum subspecies infantis CCFM111 enhanced the abundance of beneficial bacteria Akkermansia and Ruminiclostridium 6,and significantly elevated indole-3-carboxaldehyde(IAld)levels(p<0.001)in the intestines of AR mice.Concurrently with the up-regulation of IAld,which activates the aryl hydrocarbon receptor and modulates immune responses,in the CCFM111-treated AR mice,the levels of ovalbumin-specific immunoglobulin E(IgE)were markedly down-regulated(p<0.001).Similarly,total IgE levels(p<0.05)and pro-inflammatory cytokines(interleukin(IL)-4,IL-13,IL-6,and IL-17;p<0.01)were significantly reduced.Conversely,the concentration of the anti-inflammatory cytokine IL-10 was significantly increased(p<0.01).Collectively,these alterations effectively mitigated allergic symptoms,lung inflammation,and prevented body weight loss in AR mice.In conclusion,our findings suggested that IAld may serve as an efficacious material foundation for alleviating AR through the administration of probiotics.This provided a strong scientific rationale for the development of probiotic resources with AR-mitigating properties.
基金supported by the National Natural Science Foun-dation of China(32272290 and 31972973)the Fundamental Research Funds for the Central Universities(JUSRP22004).
文摘Reducing intestinal oxidative stress presents a promising approach to mitigating intestinal senescence.Lactiplantibacillus plantarum CCFM8610 has been reported to attenuate cadmium-induced intestinal dysfunction and demonstrate significant antioxidant properties.However,its effectiveness and underlying mechanisms in modulating intestinal senescence are not fully elucidated.In this study,we initially observed a marked increase in intestinal permeability and a notable elevation in oxidative stress levels in induced senescent mice.Supplementation with CCFM8610 notably alleviated these symptoms and enhanced intestinal barrier integrity.Subsequently,analysis of different parts(<3 kDa and>3 kDa)of the CCFM8610 fermentation supernatant fractions and non-targeted metabolomics data identified indole-3-lactic acid(ILA)as a potentially critical component.ILA was found to significantly reduce elevated oxidative stress levels in senescent NCM460 cells and mitigate cellular senescence.Mechanically,ILA activated adenosine 5′-monophosphate-activated protein kinase(AMPK)signaling,promoted nuclear Factor erythroid 2-Related Factor 2(NRF2)nuclear expression,reduced oxidative stress,and improved cell viability,thereby alleviating intestinal senescence.These findings indicate that CCFM8610 shows significant potential in addressing intestinal senescence,with ILA identified as a key bioactive compound responsible for these effects.
基金supported by the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.
文摘This study investigated the effects of Lactiplantibacillus plantarum CCFM1296 postbiotics(non-viable microbial cells or their components and fermentation supernatant)on skin aging via endogenous hyaluronic acid(HA)regulation.In vitro screening revealed that L.plantarum CCFM1296 postbiotic significantly upregulated HA synthase mRNA expression in human epidermal keratinocyte(HaCaT)cells.In a D-galactose-induced aging model(n=5),oral administration of CCFM1296 postbiotic increased skin HA content by 13.56%(P=0.0009),hydration by 19.77%(P=0.0121),and elasticity by 31.32%(P=0.0122)compared to the model group.Bacterial components(CCFM1296-B),rather than the fermentation supernatant,upregulated UGDH and HAS2(both mRNA and protein),reduced oxidative stress and inflammation.Skin metabolomics identified 24 bacterial metabolites that were enriched in skin tissue following CCFM1296-B intervention.These findings demonstrate that L.plantarum CCFM1296 postbiotics ameliorate skin aging,which is associated with enhanced endogenous HA synthesis.
