Cold stress represents a critical constraint on crop productivity,particularly in temperate climates.Despite the established role of abscisic acid(ABA)in cold stress responses,the precise mechanisms through which tran...Cold stress represents a critical constraint on crop productivity,particularly in temperate climates.Despite the established role of abscisic acid(ABA)in cold stress responses,the precise mechanisms through which transcription factors mediate ABAdependent cold tolerance remain elusive.Here,we identify VaMYB4a,a MYB transcription factor from Vitis amurensis Rupr.(Amur grape),as a key regulator of cold tolerance.It integrates ABA signaling with the CBF(C-repeat binding factors)-COR(cold-regulated)pathway to orchestrate cold stress adaptation.Through a combination of overexpression and CRISPR/Cas9-mediated knockout lines in Arabidopsis thaliana,grape callus,and Vitis vinifera L.seedlings,we demonstrate that VaMYB4a enhances freezing tolerance by promoting osmotic regulation,reactive oxygen species(ROS)scavenging,and stomatal closure.VaMYB4a functions as a homo-dimer,with its C-terminal domain being essential for transcriptional activation.Mechanistically,VaMYB4a directly upregulates CBF and COR genes while fine-tuning ABA signaling components such as ABI1 and ABF4.Notably,ABA exhibits a dual role:enhancing VaMYB4a-mediated freezing tolerance under short-term stress but attenuating its effects during prolonged cold exposure,revealing an intricate regulatory crosstalk between cold and hormonal pathways.Our work not only advances the molecular understanding of cold adaptation but also provides a promising genetic target for developing stress-resilient grape varieties to mitigate the impacts of climate change.展开更多
【目的】独行菜在种子萌发和幼苗生长阶段可耐受一定程度的低温胁迫,研究独行菜的低温耐受机制。【方法】设计兼并引物,从独行菜冷诱导叶片的cDNA中克隆获得LaCBF3和LaCOR15a基因核心片段。利用半定量RT-PCR法分析两个基因在不同胁迫处...【目的】独行菜在种子萌发和幼苗生长阶段可耐受一定程度的低温胁迫,研究独行菜的低温耐受机制。【方法】设计兼并引物,从独行菜冷诱导叶片的cDNA中克隆获得LaCBF3和LaCOR15a基因核心片段。利用半定量RT-PCR法分析两个基因在不同胁迫处理下的表达情况。【结果】获得442 bp LaCBF3及405 bp LaCOR15a核心片段,经比对与拟南芥CBF3、COR15a基因核心片段相似性分别为84.38%、81.8%。半定量RT-PCR结果显示在独行菜幼苗中,LaCOR15a基因不仅响应低温胁迫,还能迅速响应高盐、干旱及ABA处理,不同条件下表达各有差异。LaCBF3仅对低温胁迫响应迅速。【结论】LaCBF3、LaCOR15a在独行菜幼苗响应低温胁迫的分子机制中具有重要的调控作用,且LaCOR15a基因也应答干旱、高盐等非生物胁迫。展开更多
Low temperature as abiotic stress adversely impacts plant growth and development, and limits the ecological distribution of plants as well. Throughout their long evolutionary history, plants have developed a range of ...Low temperature as abiotic stress adversely impacts plant growth and development, and limits the ecological distribution of plants as well. Throughout their long evolutionary history, plants have developed a range of complicated and precise molecular regulatory mechanisms to deal with low-temperature stress, involving the activation of signal transduction pathways and the regulation of related genes. In this review, we provide a systematic summary of the most recent research findings regarding three hypotheses of cellular perception of low-temperature signals and two major intracellular low-temperature signaling pathways, including CBF-dependent signaling pathways and CBF-independent signaling pathways. Focus is placed on the functions of each component of the ICE-CBF-COR signaling cascade as well as their interrelationships. This review concludes that although some progress has been made in the identification, function, and mechanism of low-temperature response genes, their roles in the low-temperature regulatory network and molecular mechanisms still need to be studied in detail, which will be of great significance for improving the low-temperature tolerance of plants and adapting to climate change.展开更多
The transcription factors CBF1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating the expression levels of cold responsive （COR） genes. In this study, we ob...The transcription factors CBF1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating the expression levels of cold responsive （COR） genes. In this study, we obtained CRISPR/Casg-mediated loss-of-function mutants of cbf1-3. Over 3,ooo COR genes identified by RNA-seq analysis showed a slight but significant change in their expression levels in the mutants compared to the wild- type plants after being treated at 4 ℃ for 12 h. The C-repeat （CRT） motif （5＇-CCGAC-3＇） was enriched in promoters of genes that were up-regulated by CBF2 and CBF3 but not in promoters of genes up-regulated by CBFI. These data suggest that CBF2 and CBF3 play a more important role in directing the cold response by regulating different sets of downstream COR genes. More than 2/3 of COR genes were co-regulated by two or three CBFs and were involved mainly in cellular signal transduction and metabolic processes; less than 1[3 of the genes were regulated by one CBF, and those genes up-regulated were enriched in cold-related abiotic stress responses. Our results indicate that CBFs play an important role in the trade-off between cold tolerance and plant growth through the precise regulation of COR genes in the complicated transcriptional network.展开更多
基金supported by the Ningxia Hui Autonomous Region Key R&D Program,China(2023BCF01003)the National Natural Science Foundation of China(32472711 and 32060672)the Agricultural Breeding Project of Ningxia Hui Autonomous Region,China(NXNYYZ202101)。
文摘Cold stress represents a critical constraint on crop productivity,particularly in temperate climates.Despite the established role of abscisic acid(ABA)in cold stress responses,the precise mechanisms through which transcription factors mediate ABAdependent cold tolerance remain elusive.Here,we identify VaMYB4a,a MYB transcription factor from Vitis amurensis Rupr.(Amur grape),as a key regulator of cold tolerance.It integrates ABA signaling with the CBF(C-repeat binding factors)-COR(cold-regulated)pathway to orchestrate cold stress adaptation.Through a combination of overexpression and CRISPR/Cas9-mediated knockout lines in Arabidopsis thaliana,grape callus,and Vitis vinifera L.seedlings,we demonstrate that VaMYB4a enhances freezing tolerance by promoting osmotic regulation,reactive oxygen species(ROS)scavenging,and stomatal closure.VaMYB4a functions as a homo-dimer,with its C-terminal domain being essential for transcriptional activation.Mechanistically,VaMYB4a directly upregulates CBF and COR genes while fine-tuning ABA signaling components such as ABI1 and ABF4.Notably,ABA exhibits a dual role:enhancing VaMYB4a-mediated freezing tolerance under short-term stress but attenuating its effects during prolonged cold exposure,revealing an intricate regulatory crosstalk between cold and hormonal pathways.Our work not only advances the molecular understanding of cold adaptation but also provides a promising genetic target for developing stress-resilient grape varieties to mitigate the impacts of climate change.
文摘【目的】独行菜在种子萌发和幼苗生长阶段可耐受一定程度的低温胁迫,研究独行菜的低温耐受机制。【方法】设计兼并引物,从独行菜冷诱导叶片的cDNA中克隆获得LaCBF3和LaCOR15a基因核心片段。利用半定量RT-PCR法分析两个基因在不同胁迫处理下的表达情况。【结果】获得442 bp LaCBF3及405 bp LaCOR15a核心片段,经比对与拟南芥CBF3、COR15a基因核心片段相似性分别为84.38%、81.8%。半定量RT-PCR结果显示在独行菜幼苗中,LaCOR15a基因不仅响应低温胁迫,还能迅速响应高盐、干旱及ABA处理,不同条件下表达各有差异。LaCBF3仅对低温胁迫响应迅速。【结论】LaCBF3、LaCOR15a在独行菜幼苗响应低温胁迫的分子机制中具有重要的调控作用,且LaCOR15a基因也应答干旱、高盐等非生物胁迫。
文摘Low temperature as abiotic stress adversely impacts plant growth and development, and limits the ecological distribution of plants as well. Throughout their long evolutionary history, plants have developed a range of complicated and precise molecular regulatory mechanisms to deal with low-temperature stress, involving the activation of signal transduction pathways and the regulation of related genes. In this review, we provide a systematic summary of the most recent research findings regarding three hypotheses of cellular perception of low-temperature signals and two major intracellular low-temperature signaling pathways, including CBF-dependent signaling pathways and CBF-independent signaling pathways. Focus is placed on the functions of each component of the ICE-CBF-COR signaling cascade as well as their interrelationships. This review concludes that although some progress has been made in the identification, function, and mechanism of low-temperature response genes, their roles in the low-temperature regulatory network and molecular mechanisms still need to be studied in detail, which will be of great significance for improving the low-temperature tolerance of plants and adapting to climate change.
基金supported by grants from the National Natural Science Foundation of China (91331201)the Ministry of Agriculture of China (2014ZX08009-001) to HG
文摘The transcription factors CBF1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating the expression levels of cold responsive （COR） genes. In this study, we obtained CRISPR/Casg-mediated loss-of-function mutants of cbf1-3. Over 3,ooo COR genes identified by RNA-seq analysis showed a slight but significant change in their expression levels in the mutants compared to the wild- type plants after being treated at 4 ℃ for 12 h. The C-repeat （CRT） motif （5＇-CCGAC-3＇） was enriched in promoters of genes that were up-regulated by CBF2 and CBF3 but not in promoters of genes up-regulated by CBFI. These data suggest that CBF2 and CBF3 play a more important role in directing the cold response by regulating different sets of downstream COR genes. More than 2/3 of COR genes were co-regulated by two or three CBFs and were involved mainly in cellular signal transduction and metabolic processes; less than 1[3 of the genes were regulated by one CBF, and those genes up-regulated were enriched in cold-related abiotic stress responses. Our results indicate that CBFs play an important role in the trade-off between cold tolerance and plant growth through the precise regulation of COR genes in the complicated transcriptional network.
