Age related cataract is the leading cause of blindness associated with accumulation of oxidative stress in the eye lens.The present investigation reveals the rational of the beneficial effects of the natural compound ...Age related cataract is the leading cause of blindness associated with accumulation of oxidative stress in the eye lens.The present investigation reveals the rational of the beneficial effects of the natural compound C-phycocyanin(CPC)is beneficial when administered to rat pups to protect against the secondary effects of sodium selenite induced cataractogenesis.A single subcutaneous dose of sodium selenite(19 lmol/kg body weight)on the 10th day of postpartum is adequate to induce cataract in rat pups.Serum biochemical parameters,such as the level of electrolytes,mean activities of anti-oxidant enzymes i.e.superoxide dismutase,catalase and reduced glutathione were observed to be significantly altered during selenite induced cataractogenic process.Histopathological examination revealed signs of degradation of normal cell architecture in the liver,kidney and eye lens.Interestingly,the deleterious effects of sodium selenite toxicity were restored with the simultaneous treatment with C-PC.The results suggest that an administration of 200 mg/kg body weight of C-PC has the ability to prevent/alter the secondary changes reflected in the serum biochemical and histological modifications in rats exposed to sodium selenite.These results complement the beneficial role of C-PC of cyanobacterial origin as a efficacious anti-cataractogenic agent against sodium selenite toxicity.展开更多
<strong>Background: </strong>Numerous studies have demonstrated increasing evidence for cataractogenesis at lower levels of ionizing radiation than previously believed, with some suggesting possible absenc...<strong>Background: </strong>Numerous studies have demonstrated increasing evidence for cataractogenesis at lower levels of ionizing radiation than previously believed, with some suggesting possible absence of a threshold. Genetic differences between individuals also result in increased susceptibility in some operators, who might not be aware. European occupational exposure limits have been reduced and operators are seeking protective measures. <strong>Objective: </strong>To evaluate the protective effect of a face-shield equipped suspended protection system (Zero-Gravity<span style="white-space:nowrap;"><sup><span style="white-space:nowrap;">®</span></sup></span>, TIDI Products, Neenah, WI) along with adherence to safety practices against radiation dose to the eye lens for an interventional radiologist performing a wide variety of procedures. <strong>Materials and Methods:</strong> In this institutional review board-approved single-institution study, one interventional radiologist wore a highly sensitive personnel monitoring dosimeter badge on the cap near the left eye while performing 299 procedures (3690 fluoroscopy minutes) over 18 consecutive months while utilizing the suspended protection system along with adherence to other generally recommended safety practices and movement away from the field during angiographic power injections. Dosimetry reports and procedural information were retrospectively reviewed. <strong>Results: </strong>Total lens dose equivalent to the left eye over 18 months was 0.11 mSv (annualized dose = 0.073 mSv, or 7.3 mRem). The patient-dose-area product standardized dose of 0.00576 <span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">μ</span></span></span>Sv/Gy<span style="white-space:nowrap;"><span style="white-space:nowrap;">⋅</span></span>cm<sup>2</sup> is well below reports of conventional lead aprons, shields, and protective eyewear. <strong>Conclusion: </strong>Eye exposures were kept to near-background levels using the materials and methods of this study while performing a wide variety of complex procedures from all positions around the patient.展开更多
Objective To determine whether low power density microwave radiation can induce irreversible changes in rabbit lens epithelial cells (LECs) and the mechanisms of the changes.Methods One eye of each rabbit was exposed ...Objective To determine whether low power density microwave radiation can induce irreversible changes in rabbit lens epithelial cells (LECs) and the mechanisms of the changes.Methods One eye of each rabbit was exposed to 5mW/cm2 or 10mW/cm2 power density microwaves for 3 hours, while the contralateral eye served as a control. Annexin Ⅴ-propidium iodide (PI) two-color flow cytometry (FCM) was used to detect the early changes in rabbit lens epithelial cells after radiation. Results Lots of rabbit LECs were in the initial phase of apoptosis in the 5mW/cm2 microwave radiation group. A large number of cells became secondary necrotic cells, and severe damage could be found in the group exposed to 10mW/cm2 microwave radiation. Conclusion Low power densities of microwave radiation (5mW/cm2 and 10mW/cm2) can induce irreversible damage to rabbit LECs. This may be the non-thermal effect of microwave radiation.展开更多
文摘Age related cataract is the leading cause of blindness associated with accumulation of oxidative stress in the eye lens.The present investigation reveals the rational of the beneficial effects of the natural compound C-phycocyanin(CPC)is beneficial when administered to rat pups to protect against the secondary effects of sodium selenite induced cataractogenesis.A single subcutaneous dose of sodium selenite(19 lmol/kg body weight)on the 10th day of postpartum is adequate to induce cataract in rat pups.Serum biochemical parameters,such as the level of electrolytes,mean activities of anti-oxidant enzymes i.e.superoxide dismutase,catalase and reduced glutathione were observed to be significantly altered during selenite induced cataractogenic process.Histopathological examination revealed signs of degradation of normal cell architecture in the liver,kidney and eye lens.Interestingly,the deleterious effects of sodium selenite toxicity were restored with the simultaneous treatment with C-PC.The results suggest that an administration of 200 mg/kg body weight of C-PC has the ability to prevent/alter the secondary changes reflected in the serum biochemical and histological modifications in rats exposed to sodium selenite.These results complement the beneficial role of C-PC of cyanobacterial origin as a efficacious anti-cataractogenic agent against sodium selenite toxicity.
文摘<strong>Background: </strong>Numerous studies have demonstrated increasing evidence for cataractogenesis at lower levels of ionizing radiation than previously believed, with some suggesting possible absence of a threshold. Genetic differences between individuals also result in increased susceptibility in some operators, who might not be aware. European occupational exposure limits have been reduced and operators are seeking protective measures. <strong>Objective: </strong>To evaluate the protective effect of a face-shield equipped suspended protection system (Zero-Gravity<span style="white-space:nowrap;"><sup><span style="white-space:nowrap;">®</span></sup></span>, TIDI Products, Neenah, WI) along with adherence to safety practices against radiation dose to the eye lens for an interventional radiologist performing a wide variety of procedures. <strong>Materials and Methods:</strong> In this institutional review board-approved single-institution study, one interventional radiologist wore a highly sensitive personnel monitoring dosimeter badge on the cap near the left eye while performing 299 procedures (3690 fluoroscopy minutes) over 18 consecutive months while utilizing the suspended protection system along with adherence to other generally recommended safety practices and movement away from the field during angiographic power injections. Dosimetry reports and procedural information were retrospectively reviewed. <strong>Results: </strong>Total lens dose equivalent to the left eye over 18 months was 0.11 mSv (annualized dose = 0.073 mSv, or 7.3 mRem). The patient-dose-area product standardized dose of 0.00576 <span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">μ</span></span></span>Sv/Gy<span style="white-space:nowrap;"><span style="white-space:nowrap;">⋅</span></span>cm<sup>2</sup> is well below reports of conventional lead aprons, shields, and protective eyewear. <strong>Conclusion: </strong>Eye exposures were kept to near-background levels using the materials and methods of this study while performing a wide variety of complex procedures from all positions around the patient.
文摘Objective To determine whether low power density microwave radiation can induce irreversible changes in rabbit lens epithelial cells (LECs) and the mechanisms of the changes.Methods One eye of each rabbit was exposed to 5mW/cm2 or 10mW/cm2 power density microwaves for 3 hours, while the contralateral eye served as a control. Annexin Ⅴ-propidium iodide (PI) two-color flow cytometry (FCM) was used to detect the early changes in rabbit lens epithelial cells after radiation. Results Lots of rabbit LECs were in the initial phase of apoptosis in the 5mW/cm2 microwave radiation group. A large number of cells became secondary necrotic cells, and severe damage could be found in the group exposed to 10mW/cm2 microwave radiation. Conclusion Low power densities of microwave radiation (5mW/cm2 and 10mW/cm2) can induce irreversible damage to rabbit LECs. This may be the non-thermal effect of microwave radiation.
